FastQCFastQC Report
Fri 27 May 2016
SRR2139476_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139476_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5384370
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79350.050.305011
GTATCAA143250.036.242511
TCAACGC165850.030.6665574
ATCAACG167100.030.4626313
TATCAAC171500.029.7323932
CAACGCA171550.029.6721555
AACGCAG176450.028.8958876
ACGCAGA200650.025.4108127
CGCAGAG205800.024.7340058
GTGGTAT39900.021.844761
TGGTATC36300.021.6912612
GCAGAGT237950.021.3744419
GAGTACT127650.020.61449812-13
GTACATG183750.020.3028131
TACATGG183500.019.733872
CAGAGTA232450.019.3896810-11
ACATGGG187050.018.952743
GTACTTT141550.018.47137814-15
AGAGTAC214100.017.8559410-11
CATGGGG132000.017.096394
Produced by FastQC (version 0.11.5)