##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139476_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5384370
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.172434472370956	30.0	18.0	33.0	18.0	34.0
2	29.96534914948267	32.0	27.0	33.0	25.0	33.0
3	31.894278624983052	33.0	31.0	33.0	29.0	34.0
4	32.03271747669644	33.0	32.0	33.0	31.0	33.0
5	32.74307189141905	33.0	33.0	33.0	32.0	34.0
6	36.48030651682555	38.0	37.0	38.0	34.0	38.0
7	37.11311184038244	38.0	38.0	38.0	36.0	38.0
8	36.77315600525224	38.0	37.0	38.0	34.0	38.0
9	37.254766481501086	38.0	38.0	38.0	36.0	38.0
10-11	37.41543133179927	38.0	38.0	38.0	37.0	38.0
12-13	37.4350324179059	38.0	38.0	38.0	37.0	38.0
14-15	37.39241712586616	38.0	38.0	38.0	36.5	38.0
16-17	37.45503995824953	38.0	38.0	38.0	37.0	38.0
18-19	37.47414553606086	38.0	38.0	38.0	37.0	38.0
20-21	37.498339153836014	38.0	38.0	38.0	37.0	38.0
22-23	37.50981626067506	38.0	38.0	38.0	37.0	38.0
24-25	37.52287302037303	38.0	38.0	38.0	37.0	38.0
26-27	37.49739637153945	38.0	38.0	38.0	37.0	38.0
28-29	37.47766521044983	38.0	38.0	38.0	37.0	38.0
30-31	37.48095347105913	38.0	38.0	38.0	37.0	38.0
32-33	37.47142783573507	38.0	38.0	38.0	37.0	38.0
34-35	37.370511332969855	38.0	38.0	38.0	37.0	38.0
36-37	37.41200205408717	38.0	38.0	38.0	37.0	38.0
38-39	37.41479205589803	38.0	38.0	38.0	37.0	38.0
40-41	37.40758642283186	38.0	38.0	38.0	37.0	38.0
42-43	37.371288472102044	38.0	38.0	38.0	37.0	38.0
44-45	37.33411997814254	38.0	38.0	38.0	37.0	38.0
46-47	37.29756415589579	38.0	38.0	38.0	37.0	38.0
48-49	37.23157052537219	38.0	38.0	38.0	37.0	38.0
50-51	37.18647486010853	38.0	38.0	38.0	37.0	38.0
52-53	37.15513525601752	38.0	38.0	38.0	36.5	38.0
54-55	37.11637468729704	38.0	38.0	38.0	36.0	38.0
56-57	37.060386141385784	38.0	38.0	38.0	36.0	38.0
58-59	37.048798315830496	38.0	38.0	38.0	36.0	38.0
60-61	37.0445523083139	38.0	38.0	38.0	36.0	38.0
62-63	37.046949718270454	38.0	38.0	38.0	36.0	38.0
64-65	37.05069351856307	38.0	38.0	38.0	36.0	38.0
66-67	37.047058421388115	38.0	38.0	38.0	36.0	38.0
68-69	37.03430421391208	38.0	38.0	38.0	36.0	38.0
70-71	37.02607689740071	38.0	38.0	38.0	36.0	38.0
72-73	37.01119703454761	38.0	38.0	38.0	36.0	38.0
74-75	37.007061810428866	38.0	38.0	38.0	36.0	38.0
76-77	36.9912691436609	38.0	38.0	38.0	36.0	38.0
78-79	36.97360729150434	38.0	38.0	38.0	36.0	38.0
80-81	36.96205884417863	38.0	38.0	38.0	36.0	38.0
82-83	36.903494912313704	38.0	38.0	38.0	35.0	38.0
84-85	36.89268617239554	38.0	38.0	38.0	35.0	38.0
86-87	36.87990958109557	38.0	38.0	38.0	35.0	38.0
88-89	36.85936276149329	38.0	38.0	38.0	35.0	38.0
90-91	36.846288648681295	38.0	38.0	38.0	35.0	38.0
92-93	36.81821464199379	38.0	38.0	38.0	35.0	38.0
94-95	36.801478824330346	38.0	38.0	38.0	35.0	38.0
96-97	36.783920887794466	38.0	38.0	38.0	35.0	38.0
98-99	36.8032953104984	38.0	38.0	38.0	35.0	38.0
100	35.308028220019374	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	23.0
21	88.0
22	270.0
23	784.0
24	1928.0
25	3936.0
26	7255.0
27	12144.0
28	19660.0
29	29369.0
30	39963.0
31	51362.0
32	66385.0
33	90667.0
34	139469.0
35	268380.0
36	906630.0
37	3746052.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.42486864758551	18.25946210977329	13.72708413426269	22.58858510837851
2	14.99506227550627	20.118575966570944	42.97824373767078	21.908118020252008
3	18.809553746556897	26.047760560903082	29.948310992833445	25.19437469970657
4	12.930426400860268	18.0181711137979	38.59500740105156	30.45639508429027
5	14.125886593974782	37.10292940492574	35.18173899639141	13.589445004708073
6	30.522865256288107	37.12322890143137	18.84389445747599	13.510011384804535
7	27.457269838439778	32.49529657137232	22.654498112128252	17.39293547805964
8	24.82357267424044	36.53407919589478	20.8757570523571	17.766591077507677
9	26.04276823472384	17.60677665167884	21.31064544227087	35.03980967132645
10-11	24.860271489515025	26.6767514119572	28.366716997531743	20.09626010099603
12-13	25.782292078739015	24.194622583514878	28.636154276173443	21.386931061572664
14-15	22.895343373505163	25.47435447415389	26.535722842226665	25.094579310114273
16-17	22.24278792133527	28.687005164949657	27.13690738192212	21.933299531792947
18-19	22.20972927194825	27.71299149204085	29.014322938431054	21.062956297579845
20-21	23.12596310123193	26.777339074223033	28.965506930428642	21.131190894116394
22-23	22.911003102053886	26.58523237358153	28.84522856415572	21.658535960208866
24-25	22.601763834328448	26.79095627415748	28.902088963325447	21.705190928188628
26-27	22.486933416411066	26.91583855982744	28.8890275994743	21.708200424287195
28-29	22.434033004892555	26.899599781041545	28.969205041619666	21.69716217244623
30-31	22.69968942100291	26.815949479844363	28.82925261975449	21.655108479398237
32-33	22.448867520419242	27.016172792221425	28.781256328277177	21.75370335908216
34-35	22.661024772639117	27.029387374022452	28.743482533245206	21.56610532009323
36-37	22.617443309497762	26.887048475430873	28.761936807837053	21.73357140723431
38-39	22.480033080648642	27.03746356982221	28.82505165068976	21.657451698839388
40-41	22.714030518071173	26.91093200389657	28.702762916730002	21.672274561302252
42-43	22.588290379700805	26.9984365232768	28.525299394518573	21.887973702503817
44-45	22.673103859993077	27.062848098962505	28.435876618977158	21.828171422067257
46-47	22.715259274551673	27.123551274374165	28.168682561178187	21.99250688989597
48-49	22.7354728053	27.164011180247332	28.097922715099138	22.00259329935353
50-51	22.664837724385798	27.367701199510986	27.992472453018348	21.974988623084872
52-53	22.884645874749744	27.25430025667206	27.87519223276155	21.985861635816654
54-55	22.846885384032763	27.194510195529155	27.88824196372161	22.07036245671647
56-57	22.796071699860857	27.283620939074748	27.885103559536383	22.035203801528013
58-59	22.797345415888284	27.20611214895396	27.97496103788872	22.021581397269042
60-61	22.810299228136124	27.20929299727709	28.02227918895167	21.958128585635112
62-63	22.827092103918584	27.293157166785043	28.006137149350057	21.873613579946316
64-65	22.831850931666473	27.22471146280964	28.00537862739517	21.938058978128712
66-67	23.014141500762413	27.25702050343421	27.805283031003036	21.92355496480034
68-69	22.821568491488403	27.25097944041882	27.979102381860415	21.948349686232362
70-71	23.018359472536172	27.09156991124245	27.947854456936135	21.94221615928525
72-73	23.14025994454605	27.05697489063741	27.855695661276727	21.947069503539815
74-75	22.803526340086698	27.130495270018855	27.9331502565478	22.132828133346646
76-77	22.767555135388744	27.185187414942813	27.831254239421	22.216003210247443
78-79	22.828400814693044	27.15658348978498	27.690499047556266	22.324516647965716
80-81	22.748984498407125	27.220052156472196	27.720248869551263	22.31071447556942
82-83	22.7744181398176	27.17361932947527	27.80313674322421	22.24882578748292
84-85	22.796105211407145	27.214522909973702	27.690695942191557	22.298675936427593
86-87	22.671789294955012	27.30762486376488	27.7693110473273	22.251274793952806
88-89	22.757789870606143	27.313517497441875	27.68314665910518	22.245545972846802
90-91	22.763127450965946	27.319817927893276	27.682203512639774	22.234851108501005
92-93	22.82845320042166	27.30870026570064	27.655563719649756	22.207282814227945
94-95	22.678759841944395	27.379425158897075	27.673902484004483	22.267912515154052
96-97	22.80122009960028	27.455650394655922	27.7083681001222	22.034761405621595
98-99	23.828433546914063	29.35121193802556	27.964183592388014	18.856170922672362
100	30.46393667856248	40.314038446280705	29.222024875156816	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	818.0
1	777.0
2	959.0
3	1320.5
4	1715.0
5	2462.0
6	3190.5
7	4160.5
8	5425.5
9	6229.0
10	6270.0
11	6162.5
12	6733.0
13	8247.5
14	11398.5
15	15249.5
16	18602.5
17	21132.5
18	21772.5
19	20436.0
20	18509.0
21	16832.5
22	16547.0
23	18716.5
24	23018.5
25	29536.5
26	38182.0
27	48038.0
28	58823.0
29	70788.0
30	82398.0
31	94252.5
32	108100.0
33	122533.5
34	135688.5
35	148495.0
36	163302.0
37	175907.0
38	186365.0
39	195503.5
40	203629.5
41	211592.0
42	216840.0
43	221892.0
44	227573.0
45	232469.5
46	234614.5
47	235189.5
48	234720.0
49	232188.5
50	228699.0
51	222936.5
52	214068.5
53	202395.0
54	188934.0
55	176090.0
56	161278.5
57	145577.0
58	130139.5
59	112271.0
60	94335.5
61	77202.0
62	61760.0
63	48229.0
64	36550.0
65	27438.0
66	20518.5
67	15846.5
68	12255.0
69	9258.5
70	6951.5
71	5007.5
72	3523.0
73	2429.0
74	1624.0
75	1071.5
76	723.0
77	455.5
78	292.5
79	194.0
80	123.5
81	80.5
82	50.0
83	33.5
84	27.0
85	20.0
86	12.0
87	5.5
88	4.0
89	4.5
90	2.5
91	0.5
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005775977505260597
3	9.843305716360504E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	8.357626942834853E-5
22-23	9.28675148318708E-6
24-25	1.1145038465707134E-4
26-27	1.5790689029502393E-4
28-29	0.0015982113413351069
30-31	0.0025580160362490404
32-33	0.0020231430786364015
34-35	6.07838100358185E-4
36-37	9.393697862041334E-6
38-39	1.8911869647290794E-5
40-41	1.2381545515942335E-4
42-43	0.0019477115253095038
44-45	1.451821731390331E-4
46-47	4.789718175915009E-4
48-49	0.0012535965636055648
50-51	0.0012355770049952187
52-53	0.0
54-55	0.0030793756687385937
56-57	0.05221510579945311
58-59	0.06780483347178282
60-61	0.04456143281408521
62-63	0.007621444031321297
64-65	0.0
66-67	0.0
68-69	2.0585541647313548E-4
70-71	0.001774878565985767
72-73	0.00784225286214538
74-75	0.012278487742498734
76-77	0.013430809099322248
78-79	0.004142878494867065
80-81	0.00826856196621308
82-83	0.046577519052841757
84-85	0.03917769502190858
86-87	0.008952690960778345
88-89	0.0023406752108184312
90-91	0.00268945061155528
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	30.0
20-21	221.0
22-23	471.0
24-25	505.0
26-27	1402.0
28-29	3784.0
30-31	11701.0
32-33	14752.0
34-35	21254.0
36-37	33150.0
38-39	38051.0
40-41	37631.0
42-43	43181.0
44-45	50263.0
46-47	50236.0
48-49	48657.0
50-51	42892.0
52-53	39635.0
54-55	43060.0
56-57	44184.0
58-59	46500.0
60-61	45618.0
62-63	44140.0
64-65	46577.0
66-67	48941.0
68-69	51047.0
70-71	50556.0
72-73	47175.0
74-75	47390.0
76-77	49696.0
78-79	51184.0
80-81	51176.0
82-83	48307.0
84-85	46932.0
86-87	48629.0
88-89	50877.0
90-91	51817.0
92-93	50244.0
94-95	57159.0
96-97	165885.0
98-99	1213466.0
100-101	2545994.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.96255019898234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.0741677561663	81.91152771037467
2	5.888171762727955	10.47653552043832
3	1.2042659655881753	3.2140371424969194
4	0.4174732061115796	1.4855792422172602
5	0.16070419130800936	0.7148327343212821
6	0.08918522921254327	0.4760487258497176
7	0.04932439068228605	0.30716165074749624
8	0.032710560310588194	0.23280118909340333
9	0.0170642975268309	0.13662750828069575
>10	0.06497437214806344	0.8775965470101534
>50	0.0013560842873649314	0.08008013790758428
>100	6.021839303053196E-4	0.08717189126255528
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.857227493652925E-5	0.0
2	0.0	1.857227493652925E-5	0.0	1.857227493652925E-5	0.0
3	0.0	1.857227493652925E-5	0.0	3.71445498730585E-5	0.0
4	0.0	1.857227493652925E-5	0.0	5.571682480958775E-5	0.0
5	0.0	1.857227493652925E-5	0.0	5.571682480958775E-5	0.0
6	1.6715047442876325E-4	1.857227493652925E-5	0.0	5.571682480958775E-5	1.857227493652925E-5
7	1.6715047442876325E-4	1.857227493652925E-5	0.0	5.571682480958775E-5	1.857227493652925E-5
8	1.6715047442876325E-4	1.857227493652925E-5	0.0	5.571682480958775E-5	3.71445498730585E-5
9	1.6715047442876325E-4	5.571682480958775E-5	0.0	7.4289099746117E-5	3.71445498730585E-5
10-11	1.6715047442876325E-4	5.571682480958775E-5	0.0	1.114336496191755E-4	3.71445498730585E-5
12-13	1.6715047442876325E-4	5.571682480958775E-5	0.0	1.48578199492234E-4	3.71445498730585E-5
14-15	1.6715047442876325E-4	1.114336496191755E-4	0.0	1.5786433696049864E-4	3.71445498730585E-5
16-17	3.5287322379405574E-4	1.3000592455570474E-4	0.0	1.9500888683355715E-4	3.71445498730585E-5
18-19	3.71445498730585E-4	1.48578199492234E-4	0.0	2.1358116177008638E-4	4.643068734132313E-5
20-21	3.71445498730585E-4	1.9500888683355715E-4	0.0	2.22867299238351E-4	5.571682480958775E-5
22-23	3.807316361988496E-4	2.0429502430182177E-4	0.0	2.785841240479387E-4	6.500296227785237E-5
24-25	4.178761860719082E-4	2.2286729923835102E-4	0.0	3.157286739209973E-4	9.286137468264626E-5
26-27	4.2716232354017276E-4	2.4143957417488025E-4	0.0	4.828791483497605E-4	9.286137468264626E-5
28-29	4.2716232354017276E-4	2.4143957417488025E-4	0.0	9.471860217629918E-4	9.286137468264626E-5
30-31	4.2716232354017276E-4	2.7858412404793877E-4	0.0	0.0025629739412410366	1.114336496191755E-4
32-33	4.2716232354017276E-4	2.7858412404793877E-4	0.0	0.005878125017411508	1.6715047442876325E-4
34-35	4.643068734132312E-4	3.2501481138926187E-4	0.0	0.01064191353863126	1.6715047442876325E-4
36-37	4.921652858180252E-4	3.71445498730585E-4	0.0	0.01770866415198064	1.6715047442876325E-4
38-39	5.014514232862898E-4	3.71445498730585E-4	0.0	0.0347022957189049	1.7643661189702787E-4
40-41	5.20023698222819E-4	4.828791483497605E-4	0.0	0.053070275631132334	1.857227493652925E-4
42-43	5.20023698222819E-4	4.828791483497605E-4	0.0	0.06513296820240808	1.857227493652925E-4
44-45	5.20023698222819E-4	5.107375607545544E-4	0.0	0.07903431599240023	1.857227493652925E-4
46-47	5.20023698222819E-4	5.385959731593482E-4	0.0	0.09457002397680694	1.857227493652925E-4
48-49	5.20023698222819E-4	5.571682480958775E-4	0.0	0.10933498255134769	1.857227493652925E-4
50-51	5.478821106276129E-4	5.94312797968936E-4	0.0	0.12331061944108596	2.0429502430182177E-4
52-53	5.571682480958775E-4	5.94312797968936E-4	0.0	0.16051088613895403	2.1358116177008638E-4
54-55	5.571682480958775E-4	6.314573478419946E-4	0.0	0.20621725475775254	2.22867299238351E-4
56-57	5.571682480958775E-4	6.500296227785237E-4	0.0	0.27435893149987833	2.22867299238351E-4
58-59	5.571682480958775E-4	6.500296227785237E-4	0.0	0.32047389016728045	2.321534367066156E-4
60-61	5.94312797968936E-4	6.68601897715053E-4	0.0	0.3361396040762429	2.4143957417488025E-4
62-63	6.500296227785237E-4	6.778880351833176E-4	0.0	0.34757083929967664	2.4143957417488025E-4
64-65	6.68601897715053E-4	7.243187225246408E-4	0.0	0.35436829192644637	2.4143957417488025E-4
66-67	6.871741726515823E-4	7.243187225246408E-4	0.0	0.360952163391446	2.692979865796741E-4
68-69	6.964603101198469E-4	7.243187225246408E-4	0.0	0.36722959231999286	2.878702615162034E-4
70-71	7.336048599929053E-4	7.4289099746117E-4	0.0	0.3722998233776653	3.343009488575265E-4
72-73	8.264662346755517E-4	7.614632723976992E-4	0.0	0.3767107386750911	3.71445498730585E-4
74-75	8.357523721438162E-4	7.614632723976992E-4	0.0	0.3802951877378412	3.807316361988496E-4
76-77	8.357523721438162E-4	7.893216848024931E-4	0.0	0.38369391405122605	3.9001777366711425E-4
78-79	8.357523721438162E-4	8.264662346755517E-4	0.0	0.3857647227066491	3.993039111353789E-4
80-81	8.357523721438162E-4	8.357523721438162E-4	0.0	0.3865168998415785	4.2716232354017276E-4
82-83	8.357523721438162E-4	8.357523721438162E-4	0.0	0.3868790592028408	4.643068734132313E-4
84-85	8.357523721438162E-4	8.357523721438162E-4	0.0	0.3871390710519522	4.735930108814959E-4
86-87	8.357523721438162E-4	8.543246470803455E-4	0.0	0.38729693538891274	4.828791483497605E-4
88	8.357523721438162E-4	9.100414718899333E-4	0.0	0.387306221526381	4.828791483497605E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7935	0.0	50.30501	1
GTATCAA	14325	0.0	36.24251	1
TCAACGC	16585	0.0	30.666557	4
ATCAACG	16710	0.0	30.462631	3
TATCAAC	17150	0.0	29.732393	2
CAACGCA	17155	0.0	29.672155	5
AACGCAG	17645	0.0	28.895887	6
ACGCAGA	20065	0.0	25.410812	7
CGCAGAG	20580	0.0	24.734005	8
GTGGTAT	3990	0.0	21.84476	1
TGGTATC	3630	0.0	21.691261	2
GCAGAGT	23795	0.0	21.374441	9
GAGTACT	12765	0.0	20.614498	12-13
GTACATG	18375	0.0	20.302813	1
TACATGG	18350	0.0	19.73387	2
CAGAGTA	23245	0.0	19.38968	10-11
ACATGGG	18705	0.0	18.95274	3
GTACTTT	14155	0.0	18.471378	14-15
AGAGTAC	21410	0.0	17.85594	10-11
CATGGGG	13200	0.0	17.09639	4
>>END_MODULE