FastQCFastQC Report
Fri 27 May 2016
SRR2139475_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139475_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5165581
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127950.028.9070191
CGAGACC40200.027.96417286-87
AGACCGA42600.026.8594588-89
GTATCAA189250.026.6566771
GAGACCG42600.026.13351888-89
GACCGAG43500.026.09986390-91
ACGAGAC42700.025.80688786-87
ACCGAGG45600.025.65047590-91
CCCACGA44050.025.06842882-83
CAACGCA193050.025.0309265
AACGCAG194600.024.8970156
CACGAGA44650.024.88724584-85
ATCTCCG42300.024.57133374-75
TCAACGC199000.024.517234
ATCAACG200650.024.3579433
TCCGAGC44550.024.23579476-77
TCTCCGA44450.023.96741574-75
GCCCACG45400.023.9644382-83
CTCCGAG44650.023.59315776-77
TATCAAC211100.023.4349022
Produced by FastQC (version 0.11.5)