##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139475_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5165581
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.371058550819356	30.0	18.0	33.0	18.0	34.0
2	30.156361307663165	32.0	28.0	33.0	25.0	33.0
3	31.96274939837358	33.0	31.0	33.0	29.0	34.0
4	32.12351195344725	33.0	32.0	33.0	31.0	34.0
5	32.759119061340826	33.0	33.0	33.0	32.0	34.0
6	36.442742839576034	38.0	37.0	38.0	34.0	38.0
7	37.119267706769094	38.0	38.0	38.0	36.0	38.0
8	36.76731097624836	38.0	37.0	38.0	34.0	38.0
9	37.24572298837246	38.0	38.0	38.0	36.0	38.0
10-11	37.42506990017192	38.0	38.0	38.0	37.0	38.0
12-13	37.43466417427197	38.0	38.0	38.0	37.0	38.0
14-15	37.40432537985563	38.0	38.0	38.0	37.0	38.0
16-17	37.463961072336296	38.0	38.0	38.0	37.0	38.0
18-19	37.48154641268813	38.0	38.0	38.0	37.0	38.0
20-21	37.50316321035508	38.0	38.0	38.0	37.0	38.0
22-23	37.51494904320242	38.0	38.0	38.0	37.0	38.0
24-25	37.5304873398066	38.0	38.0	38.0	37.5	38.0
26-27	37.50173907048429	38.0	38.0	38.0	37.0	38.0
28-29	37.47919266839444	38.0	38.0	38.0	37.0	38.0
30-31	37.48075831738788	38.0	38.0	38.0	37.0	38.0
32-33	37.4691141198978	38.0	38.0	38.0	37.0	38.0
34-35	37.36576983531734	38.0	38.0	38.0	37.0	38.0
36-37	37.40364888872383	38.0	38.0	38.0	37.0	38.0
38-39	37.407824818745354	38.0	38.0	38.0	37.0	38.0
40-41	37.40139184370071	38.0	38.0	38.0	37.0	38.0
42-43	37.36430040018169	38.0	38.0	38.0	37.0	38.0
44-45	37.32924838019753	38.0	38.0	38.0	37.0	38.0
46-47	37.29325669659508	38.0	38.0	38.0	37.0	38.0
48-49	37.22755645833105	38.0	38.0	38.0	37.0	38.0
50-51	37.187494168597354	38.0	38.0	38.0	37.0	38.0
52-53	37.16089267799097	38.0	38.0	38.0	37.0	38.0
54-55	37.12527410180863	38.0	38.0	38.0	36.0	38.0
56-57	37.071654545242176	38.0	38.0	38.0	36.0	38.0
58-59	37.06355311416433	38.0	38.0	38.0	36.0	38.0
60-61	37.065305476826396	38.0	38.0	38.0	36.0	38.0
62-63	37.068534125578665	38.0	38.0	38.0	36.0	38.0
64-65	37.07405714439203	38.0	38.0	38.0	36.0	38.0
66-67	37.06788373263281	38.0	38.0	38.0	36.0	38.0
68-69	37.05943510562733	38.0	38.0	38.0	36.0	38.0
70-71	37.05381114393808	38.0	38.0	38.0	36.0	38.0
72-73	37.04154940750898	38.0	38.0	38.0	36.0	38.0
74-75	37.03609928874966	38.0	38.0	38.0	36.0	38.0
76-77	37.023831948828345	38.0	38.0	38.0	36.0	38.0
78-79	37.010448191903805	38.0	38.0	38.0	36.0	38.0
80-81	36.99448095552664	38.0	38.0	38.0	36.0	38.0
82-83	36.9420528848911	38.0	38.0	38.0	36.0	38.0
84-85	36.93337899077595	38.0	38.0	38.0	35.5	38.0
86-87	36.91819081545488	38.0	38.0	38.0	35.0	38.0
88-89	36.897588605661824	38.0	38.0	38.0	35.0	38.0
90-91	36.887656403240626	38.0	38.0	38.0	35.0	38.0
92-93	36.86847591206544	38.0	38.0	38.0	35.0	38.0
94-95	36.84640703592166	38.0	38.0	38.0	35.0	38.0
96-97	36.830664336751084	38.0	38.0	38.0	35.0	38.0
98-99	36.84743874882864	38.0	38.0	38.0	35.0	38.0
100	35.38770412029835	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	6.0
20	32.0
21	79.0
22	299.0
23	877.0
24	1936.0
25	4036.0
26	7203.0
27	12388.0
28	19636.0
29	29112.0
30	38778.0
31	49819.0
32	64088.0
33	86436.0
34	131597.0
35	248760.0
36	821913.0
37	3648586.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.10451002510656	18.69607697565869	13.732530764690361	22.46688223454438
2	14.829161386998319	20.27617223157424	43.07839593592185	21.816270445505594
3	18.822423664583948	26.20395797135819	30.129057549813425	24.84456081424444
4	12.793101105180618	18.190499771468108	38.97464776953454	30.041751353816736
5	13.80967600740362	37.39058587988457	35.394837482947224	13.40490062976459
6	30.10815240337921	37.594377089430985	18.838771476045	13.458699031144802
7	26.953850883375946	32.8310794081053	22.850072431348963	17.36499727716979
8	24.373424789970382	36.85018200276019	20.88798917295073	17.888404034318693
9	25.624745793358	17.983378829990276	21.6176070029683	34.77426837368343
10-11	24.569801538297433	26.981243736183792	28.448968276753376	19.999986448765394
12-13	25.57999768080299	24.51565467659882	28.762795511289053	21.14155213130914
14-15	22.61461973009425	25.832805641804864	26.829247281186763	24.72332734691412
16-17	21.82982901632943	29.088402640477423	27.466300499401715	21.615467843791432
18-19	21.774975554540717	28.18723585981906	29.258973966336022	20.7788146193042
20-21	22.676433498732905	27.18970349176411	29.234244729866994	20.89961827963599
22-23	22.525631204970637	26.934965246742703	29.15786281796481	21.38154073032185
24-25	22.27243012749322	27.112172013348623	29.21210191594722	21.40329594321094
26-27	22.135256077915244	27.229937773142627	29.26849229387069	21.366313855071443
28-29	22.034512574830107	27.34183093185425	29.27055006214907	21.353106431166573
30-31	22.255769148684358	27.257224289600206	29.09902524960517	21.387981312110266
32-33	22.10124852183015	27.355225397872328	29.082303350058137	21.461222730239385
34-35	22.312509537421064	27.402969386158173	28.983716788445008	21.300804287975755
36-37	22.25520476233281	27.2104291281427	29.103976176585615	21.430389932938876
38-39	22.100899359660808	27.427440031441087	29.13937912133439	21.332281487563716
40-41	22.35693087769693	27.304966156971343	28.99802020266142	21.340082762670306
42-43	22.218919512532036	27.431094805313755	28.76152082190328	21.588464860250934
44-45	22.305861833181105	27.534724984567234	28.63850266410343	21.520910518148227
46-47	22.45226010303419	27.53512975133362	28.335571579120604	21.67703856651158
48-49	22.3911376558932	27.634510688075302	28.286860927907842	21.687490728123656
50-51	22.329559180275236	27.811720567909397	28.222642806914987	21.63607744490038
52-53	22.516333239282805	27.784379858444392	28.022284238782646	21.677002663490157
54-55	22.476097766462296	27.647186553033485	28.077126165788297	21.799589514715922
56-57	22.447468117238024	27.775211619695728	28.04151133223537	21.73580893083088
58-59	22.44645662290248	27.70064616780766	28.009805927639924	21.843091281649944
60-61	22.673964135227408	27.631424822497642	28.221902937077065	21.47270810519789
62-63	22.389008619755476	27.737344529277085	28.229710024941202	21.643936826026234
64-65	22.40928642901916	27.715319208701466	28.141495519038962	21.73389884324041
66-67	22.541147637268004	27.799493329932584	28.131187479839326	21.528171552960085
68-69	22.321873442949677	28.040922067932183	28.116696660079704	21.520507829038436
70-71	22.537974130672257	27.823418378695763	27.81871298158276	21.81989450904922
72-73	22.651852527428815	27.78732577774629	27.809648879108522	21.751172815716373
74-75	22.370302557316023	27.7524920659492	28.11752271120606	21.759682665528715
76-77	22.34764961862743	27.53838810277659	28.05174904751106	22.06221323108492
78-79	22.34679976893444	27.458029088607688	27.80119216604736	22.393978976410516
80-81	22.558483933034935	27.78713137104286	27.7847957720204	21.869588923901798
82-83	22.561879718991797	27.457892534385614	27.910052434241372	22.070175312381217
84-85	22.365923264386637	27.507318167217594	27.808780976727387	22.31797759166838
86-87	22.254034803192095	27.782960176165634	27.87481104649028	22.08819397415199
88-89	22.563984617226186	27.76283186235074	27.79702185597199	21.876161664451086
90-91	22.619087878248163	27.796031001087428	27.758756349278517	21.826124771385892
92-93	22.426767095198183	27.505454292879904	27.780143040438894	22.28763557148302
94-95	22.51724218918146	27.799777432371435	27.8465702805864	21.83641009786071
96-97	22.808149484207135	27.69319671774939	27.8987908782175	21.599862919825977
98-99	23.496748325237533	29.761272517416977	28.2308532379261	18.51112591941939
100	29.80339834868972	40.69055881297116	29.50604283833912	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	780.0
1	756.0
2	966.5
3	1300.0
4	1698.0
5	2488.5
6	3209.5
7	3978.5
8	5018.0
9	5651.5
10	5744.5
11	5827.0
12	6459.5
13	8148.0
14	11626.0
15	15755.5
16	19228.0
17	21836.5
18	22489.0
19	21346.5
20	19631.0
21	18093.0
22	17779.0
23	20026.0
24	24816.5
25	31641.0
26	40872.5
27	51427.5
28	62631.0
29	75427.0
30	87465.0
31	98875.5
32	112117.0
33	125707.5
34	137994.0
35	148929.5
36	161469.5
37	173261.5
38	182232.0
39	189420.0
40	196339.0
41	203023.0
42	207125.0
43	211546.5
44	217249.5
45	222376.0
46	225727.0
47	227884.5
48	226580.5
49	219811.0
50	211556.0
51	202606.0
52	192419.5
53	181805.0
54	169333.5
55	156305.5
56	142712.5
57	127871.5
58	113691.5
59	98536.5
60	82287.0
61	66521.0
62	52598.0
63	40830.0
64	30623.5
65	22652.5
66	16636.0
67	12569.0
68	9689.0
69	7302.5
70	5339.0
71	3748.0
72	2594.5
73	1725.5
74	1094.5
75	704.0
76	440.5
77	272.0
78	191.0
79	122.0
80	70.0
81	43.0
82	31.5
83	20.5
84	17.5
85	17.5
86	15.5
87	16.5
88	13.5
89	8.5
90	6.0
91	5.5
92	4.0
93	2.5
94	1.5
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006311003544422206
3	0.0010840987683670045
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	7.744175049332815E-5
22-23	0.0
24-25	1.9366560476587815E-4
26-27	2.7118667706344733E-4
28-29	0.0020736922390711334
30-31	0.002900496760998776
32-33	0.0023527661773334314
34-35	6.142914616022008E-4
36-37	0.0
38-39	9.852432240389888E-6
40-41	3.174013714913262E-4
42-43	0.0025169210504789492
44-45	3.2224923521193227E-4
46-47	6.200839288680377E-4
48-49	0.0011900689973278846
50-51	0.001066055599457036
52-53	0.0
54-55	0.0033631419817650443
56-57	0.05353023531158211
58-59	0.0696842035201136
60-61	0.046585083514261834
62-63	0.007899156913989017
64-65	0.0
66-67	0.0
68-69	2.1169154580843495E-4
70-71	0.0015309191638659852
72-73	0.00817575020183528
74-75	0.013078161823594083
76-77	0.014613617688044494
78-79	0.004490264684103431
80-81	0.007468616694824142
82-83	0.04694454754720432
84-85	0.0396058263440758
86-87	0.009168331920545204
88-89	0.0023890337163214328
90-91	0.0026051497297282403
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	210.0
20-21	751.0
22-23	933.0
24-25	870.0
26-27	2164.0
28-29	3930.0
30-31	10665.0
32-33	14012.0
34-35	18856.0
36-37	29964.0
38-39	33954.0
40-41	34002.0
42-43	39028.0
44-45	45908.0
46-47	45656.0
48-49	43495.0
50-51	38894.0
52-53	35393.0
54-55	38377.0
56-57	40190.0
58-59	22882.0
60-61	41311.0
62-63	39412.0
64-65	41427.0
66-67	44278.0
68-69	45674.0
70-71	45629.0
72-73	42506.0
74-75	43041.0
76-77	45510.0
78-79	47244.0
80-81	46350.0
82-83	44051.0
84-85	43282.0
86-87	44987.0
88-89	46637.0
90-91	47626.0
92-93	47249.0
94-95	54818.0
96-97	177369.0
98-99	1169946.0
100-101	2507100.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.69393684303584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.72544975809018	81.35491247743107
2	6.200030727263326	10.998102674975502
3	1.247589555640564	3.3196088776204604
4	0.4114285823064405	1.4596488277802884
5	0.1693016582165548	0.7508015290640175
6	0.08568153616222345	0.45596596541919227
7	0.05170525667470098	0.32101599389712204
8	0.030926451626777332	0.2194390997891674
9	0.017740723427597735	0.14161451429134078
>10	0.05820807066615752	0.7944258461026562
>50	0.0012817450952988254	0.07610976122452744
>100	6.253488230012692E-4	0.09397662742682901
>500	3.0586007213089983E-5	0.014377804977803742
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	3.871781315596445E-5	0.0	3.871781315596445E-5	0.0
5	0.0	3.871781315596445E-5	0.0	3.871781315596445E-5	0.0
6	5.8076719733946674E-5	3.871781315596445E-5	0.0	3.871781315596445E-5	0.0
7	5.8076719733946674E-5	3.871781315596445E-5	0.0	3.871781315596445E-5	0.0
8	5.8076719733946674E-5	7.74356263119289E-5	0.0	3.871781315596445E-5	0.0
9	5.8076719733946674E-5	1.355123460458756E-4	0.0	5.8076719733946674E-5	0.0
10-11	5.8076719733946674E-5	1.355123460458756E-4	0.0	5.8076719733946674E-5	0.0
12-13	5.8076719733946674E-5	1.355123460458756E-4	0.0	5.8076719733946674E-5	0.0
14-15	5.8076719733946674E-5	2.4198633222477782E-4	0.0	6.775617302293778E-5	3.871781315596445E-5
16-17	5.8076719733946674E-5	2.516657855137689E-4	0.0	1.5487125262385782E-4	3.871781315596445E-5
18-19	5.8076719733946674E-5	2.903835986697334E-4	0.0	2.4198633222477782E-4	3.871781315596445E-5
20-21	5.8076719733946674E-5	3.2910141182569783E-4	0.0	4.0653703813762674E-4	4.839726644495556E-5
22-23	5.8076719733946674E-5	3.3878086511468893E-4	0.0	5.904466506284579E-4	6.775617302293778E-5
24-25	5.8076719733946674E-5	3.4846031840368003E-4	0.0	8.61471342720209E-4	7.74356263119289E-5
26-27	5.8076719733946674E-5	3.4846031840368003E-4	0.0	0.0011131371282339778	7.74356263119289E-5
28-29	5.8076719733946674E-5	3.4846031840368003E-4	0.0	0.0017713399518853737	7.74356263119289E-5
30-31	5.8076719733946674E-5	3.678192249816623E-4	0.0	0.0030296688794542183	8.711507960092001E-5
32-33	5.8076719733946674E-5	3.678192249816623E-4	0.0	0.00565280072077081	1.1615343946789335E-4
34-35	7.74356263119289E-5	5.420493841835022E-4	0.0	0.009176121717963575	1.355123460458756E-4
36-37	9.679453288991112E-5	7.066000900963512E-4	0.0	0.014490141573619695	1.355123460458756E-4
38-39	9.679453288991112E-5	7.162795433853424E-4	0.0	0.029019000960395357	1.451917993348667E-4
40-41	9.679453288991112E-5	7.356384499633246E-4	0.0	0.045706378430616035	1.548712526238578E-4
42-43	9.679453288991112E-5	7.646768098302979E-4	0.0	0.054698590536088776	1.6455070591284892E-4
44-45	9.679453288991112E-5	8.324329828532357E-4	0.0	0.06541374532700193	1.7423015920184002E-4
46-47	9.679453288991112E-5	8.711507960092001E-4	0.0	0.07673870567512155	1.7423015920184002E-4
48-49	9.679453288991112E-5	8.905097025871823E-4	0.0	0.08859603595413565	1.7423015920184002E-4
50-51	9.679453288991112E-5	0.0010066631420550757	0.0	0.0998822784890993	1.7423015920184002E-4
52-53	9.679453288991112E-5	0.0010066631420550757	0.0	0.1335280581216324	1.9358906577982224E-4
54-55	9.679453288991112E-5	0.001016342595344067	0.0	0.1746850935064226	2.1294797235780447E-4
56-57	1.0647398617890223E-4	0.001026022048633058	0.0	0.22658632204199297	2.1294797235780447E-4
58-59	1.1615343946789335E-4	0.001026022048633058	0.0	0.4468132432731188	2.1294797235780447E-4
60-61	1.355123460458756E-4	0.001026022048633058	0.0	0.6447871013928539	2.323068789357867E-4
62-63	1.9358906577982224E-4	0.001026022048633058	0.0	0.6519014995602623	2.323068789357867E-4
64-65	1.9358906577982224E-4	0.0010647398617890224	0.0	0.6579608373191709	2.323068789357867E-4
66-67	2.1294797235780447E-4	0.0010840987683670047	0.0	0.6645428655556849	2.4198633222477782E-4
68-69	2.323068789357867E-4	0.0010937782216559958	0.0	0.6704860498751253	2.516657855137689E-4
70-71	2.323068789357867E-4	0.0011034576749449868	0.0	0.6757516724643365	2.710246920917512E-4
72-73	2.516657855137689E-4	0.0011034576749449868	0.0	0.679507300340465	2.903835986697334E-4
74-75	2.516657855137689E-4	0.0011131371282339778	0.0	0.6824982514067633	2.903835986697334E-4
76-77	2.516657855137689E-4	0.001200252207834898	0.0	0.6857215093519974	2.903835986697334E-4
78-79	2.516657855137689E-4	0.0012389700209908623	0.0	0.6879187452485984	2.903835986697334E-4
80-81	2.613452388027601E-4	0.0012583289275688446	0.0	0.6886834220584286	3.2910141182569783E-4
82-83	2.710246920917512E-4	0.0012583289275688446	0.0	0.6891770741761672	3.678192249816623E-4
84-85	2.710246920917512E-4	0.0012583289275688446	0.0	0.689409381055103	3.678192249816623E-4
86-87	2.903835986697334E-4	0.0012583289275688446	0.0	0.6895448934011489	3.678192249816623E-4
88	3.2910141182569783E-4	0.0013164056473027913	0.0	0.6895642523077269	3.678192249816623E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12795	0.0	28.907019	1
CGAGACC	4020	0.0	27.964172	86-87
AGACCGA	4260	0.0	26.85945	88-89
GTATCAA	18925	0.0	26.656677	1
GAGACCG	4260	0.0	26.133518	88-89
GACCGAG	4350	0.0	26.099863	90-91
ACGAGAC	4270	0.0	25.806887	86-87
ACCGAGG	4560	0.0	25.650475	90-91
CCCACGA	4405	0.0	25.068428	82-83
CAACGCA	19305	0.0	25.030926	5
AACGCAG	19460	0.0	24.897015	6
CACGAGA	4465	0.0	24.887245	84-85
ATCTCCG	4230	0.0	24.571333	74-75
TCAACGC	19900	0.0	24.51723	4
ATCAACG	20065	0.0	24.357943	3
TCCGAGC	4455	0.0	24.235794	76-77
TCTCCGA	4445	0.0	23.967415	74-75
GCCCACG	4540	0.0	23.96443	82-83
CTCCGAG	4465	0.0	23.593157	76-77
TATCAAC	21110	0.0	23.434902	2
>>END_MODULE