FastQCFastQC Report
Fri 27 May 2016
SRR2139474_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139474_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3669659
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51350.048.9541361
GTATCAA97100.037.8677371
ATCTCGT78350.033.28228894
TCAACGC114700.031.2011074
ATCAACG116450.030.7688063
TATCAAC117850.030.7303262
CAACGCA118150.030.290035
AACGCAG121100.029.5873456
ACGCAGA137250.026.1058487
TATCTCG87200.025.77968894
CGCAGAG142800.025.0912288
TGGTATC26000.024.7446562
GTGGTAT29500.024.408581
GCAGAGT163400.021.7975869
GTACATG130900.021.5474641
TACATGG132000.020.6900672
ACATGGG130750.020.5283013
GAGTACT84950.019.91045812-13
CAGAGTA159500.018.69784410-11
AGAGTAC146450.018.13849410-11
Produced by FastQC (version 0.11.5)