##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139474_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3669659
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.27332457865976	30.0	18.0	33.0	18.0	34.0
2	30.129744480345447	32.0	28.0	33.0	25.0	33.0
3	31.96185122377856	33.0	31.0	33.0	29.0	34.0
4	32.11380103709909	33.0	32.0	33.0	31.0	34.0
5	32.77097790285146	33.0	33.0	33.0	32.0	34.0
6	36.47944945293282	38.0	37.0	38.0	34.0	38.0
7	37.13678382650813	38.0	38.0	38.0	36.0	38.0
8	36.76533623423866	38.0	37.0	38.0	34.0	38.0
9	37.26213525561912	38.0	38.0	38.0	36.0	38.0
10-11	37.43474298293111	38.0	38.0	38.0	37.0	38.0
12-13	37.44122955293666	38.0	38.0	38.0	37.0	38.0
14-15	37.407496445855045	38.0	38.0	38.0	37.0	38.0
16-17	37.47142132279866	38.0	38.0	38.0	37.0	38.0
18-19	37.48859825940231	38.0	38.0	38.0	37.0	38.0
20-21	37.51364328577168	38.0	38.0	38.0	37.0	38.0
22-23	37.5247831699638	38.0	38.0	38.0	37.0	38.0
24-25	37.539772910175245	38.0	38.0	38.0	37.5	38.0
26-27	37.51314920494314	38.0	38.0	38.0	37.0	38.0
28-29	37.492758754149776	38.0	38.0	38.0	37.0	38.0
30-31	37.49827297846373	38.0	38.0	38.0	37.0	38.0
32-33	37.483305635835606	38.0	38.0	38.0	37.0	38.0
34-35	37.38026100746039	38.0	38.0	38.0	37.0	38.0
36-37	37.41797571481182	38.0	38.0	38.0	37.0	38.0
38-39	37.40497665656083	38.0	38.0	38.0	37.0	38.0
40-41	37.42753020286429	38.0	38.0	38.0	37.0	38.0
42-43	37.39858883545128	38.0	38.0	38.0	37.0	38.0
44-45	37.3620380557071	38.0	38.0	38.0	37.0	38.0
46-47	37.327922399737375	38.0	38.0	38.0	37.0	38.0
48-49	37.25989656746482	38.0	38.0	38.0	37.0	38.0
50-51	37.21785051624548	38.0	38.0	38.0	37.0	38.0
52-53	37.18996013289856	38.0	38.0	38.0	37.0	38.0
54-55	37.154436488806724	38.0	38.0	38.0	36.0	38.0
56-57	37.10277516677873	38.0	38.0	38.0	36.0	38.0
58-59	37.09100327761409	38.0	38.0	38.0	36.0	38.0
60-61	37.08129767517346	38.0	38.0	38.0	36.0	38.0
62-63	37.085307576517295	38.0	38.0	38.0	36.0	38.0
64-65	37.09222436767573	38.0	38.0	38.0	36.0	38.0
66-67	37.08408459547376	38.0	38.0	38.0	36.0	38.0
68-69	37.0705701628405	38.0	38.0	38.0	36.0	38.0
70-71	37.07181134792491	38.0	38.0	38.0	36.0	38.0
72-73	37.06358442922551	38.0	38.0	38.0	36.0	38.0
74-75	37.05421953752409	38.0	38.0	38.0	36.0	38.0
76-77	37.03940124577292	38.0	38.0	38.0	36.0	38.0
78-79	37.02170891486885	38.0	38.0	38.0	36.0	38.0
80-81	37.00441347651419	38.0	38.0	38.0	36.0	38.0
82-83	36.92220714183375	38.0	38.0	38.0	35.0	38.0
84-85	36.944315560167254	38.0	38.0	38.0	35.0	38.0
86-87	36.936948478684954	38.0	38.0	38.0	35.0	38.0
88-89	36.917631008638594	38.0	38.0	38.0	35.0	38.0
90-91	36.91591199972112	38.0	38.0	38.0	35.0	38.0
92-93	36.89454777225326	38.0	38.0	38.0	35.0	38.0
94-95	36.837728918789296	38.0	38.0	38.0	35.0	38.0
96-97	36.75904767020193	38.0	38.0	38.0	35.0	38.0
98-99	36.80182574535234	38.0	38.0	38.0	35.0	38.0
100	35.36047884919871	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	14.0
21	58.0
22	178.0
23	544.0
24	1326.0
25	2632.0
26	4677.0
27	8096.0
28	13127.0
29	19542.0
30	26172.0
31	33710.0
32	43528.0
33	59473.0
34	91700.0
35	176149.0
36	599087.0
37	2589644.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.01532703719883	18.5586181168332	13.491090044061316	22.934964801906663
2	14.872044754903243	19.941036810297465	43.25883085227917	21.928087582520124
3	18.82972365871318	26.097679084179898	29.98060566885644	25.091991588250472
4	12.906403565017893	18.276466559971922	37.98481003275781	30.83231984225237
5	14.016479460353128	37.23602111258839	35.228041624576015	13.519457802482465
6	30.59385081829129	37.10456475656185	18.557255592413355	13.744328832733505
7	27.505116960458725	32.32632786861123	22.222201027397915	17.946354143532137
8	24.762845812104068	36.71376550246222	20.778524653108096	17.74486403232562
9	25.851284819652182	17.58296888075977	21.13081896710294	35.43492733248512
10-11	24.908404295875993	26.692657274149994	28.198900769799046	20.200037660174964
12-13	25.800435408303606	24.031211074380483	28.44659408408247	21.72175943323344
14-15	22.912415022758246	25.329056460014403	26.10574988030223	25.65277863692512
16-17	22.445409777856742	28.756459387643375	26.719458129488327	22.078672705011556
18-19	22.443379616471177	27.619814266121185	28.55814395833509	21.378662159072544
20-21	23.2004344878254	26.64839132681202	28.490209308460194	21.660964876902383
22-23	22.997155436664183	26.80701172053841	28.347321782847374	21.84851105995003
24-25	22.874622804416457	26.969198056025533	28.417639998430168	21.738539141127834
26-27	22.737130484781506	27.013951381339258	28.51547375535141	21.733444378527828
28-29	22.661938672227773	26.740576913847942	28.66443200806244	21.933052405861847
30-31	22.63822219553174	26.603109956747772	28.75824201321452	22.000425834505972
32-33	22.342086491074504	27.115179239121307	28.52948974769902	22.01324452210517
34-35	22.78009744763723	26.909317203602264	28.506474068110133	21.804111280650375
36-37	22.55238832228993	26.87847630099856	28.75163348313278	21.817501893578736
38-39	22.37510773803765	26.82324363780724	28.74944311667257	22.052205507482544
40-41	22.799804739106264	26.613708446929934	28.779983332271325	21.80650348169248
42-43	22.72711562260096	26.66086588533617	28.60368739736467	22.0083310946982
44-45	22.504838334291975	26.9717257580813	28.492721733482973	22.030714174143746
46-47	22.890984742617583	26.725862523195342	28.203878490756225	22.17927424343085
48-49	22.749067314458557	26.967345376948415	27.996256821062914	22.287330487530113
50-51	22.463926479083334	27.15174331445879	28.038170683692144	22.346159522765724
52-53	22.541552158990626	26.924402388513492	28.21026039528645	22.32378505720943
54-55	22.768651630547204	26.720511358542243	28.21771647798757	22.293120532922984
56-57	22.775439078145617	26.55085997075061	28.10878883712854	22.564912113975236
58-59	22.27382698673492	26.511196836399314	28.523185820447022	22.691790356418746
60-61	22.72517290972772	27.196633916545952	28.332478645997867	21.745714527728456
62-63	21.988437409084465	27.72264701274869	28.296047085854102	21.99286849231274
64-65	21.824496150943236	28.062531181435684	27.91867655552326	22.194296112097817
66-67	22.106401337275738	28.666240600804688	27.300157404560814	21.92720065735876
68-69	22.020800936877333	28.64418159007016	27.276409843078298	22.058607629974208
70-71	22.207105901955142	27.77804650076492	27.320565182360895	22.69428241491904
72-73	22.629643839932516	26.7911069242477	27.435213017616167	23.144036218203624
74-75	22.584665183996538	26.7313577026768	27.730490510814853	22.953486602511806
76-77	22.72943317716676	26.80368172526551	27.013809634078207	23.453075463489526
78-79	23.024489063774695	26.76742421643973	26.448536795630268	23.75954992415531
80-81	22.79085920193403	27.27363168214747	26.676505554055254	23.25900356186325
82-83	22.746626858043932	26.969753516036082	26.711552233687375	23.57206739223261
84-85	22.752044754620947	27.464048728034975	26.673649377488275	23.11025713985581
86-87	23.365241921237693	27.32597143452204	26.751781819631532	22.557004824608732
88-89	23.040647930155504	27.53096413094765	27.10431089965921	22.324077039237633
90-91	23.040915950669504	27.042268445162897	26.859583120502613	23.057232483664986
92-93	23.061530382052883	26.510486490994563	27.137549979479836	23.290433147472722
94-95	22.13022804561923	27.08161739068919	27.9364185049661	22.851736058725482
96-97	22.320935812176618	26.886772880775894	28.14840967082292	22.64388163622457
98-99	23.610385203327493	28.732109743618956	28.380411600743987	19.27709345230956
100	30.46600440803331	39.99103450852153	29.542961083445164	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	560.0
1	501.0
2	574.5
3	789.0
4	1086.5
5	1617.0
6	2101.5
7	2606.0
8	3285.0
9	3744.0
10	3869.5
11	3898.0
12	4224.5
13	5172.0
14	7249.5
15	9855.5
16	11979.0
17	13506.0
18	13827.5
19	12973.5
20	11662.0
21	10555.5
22	10280.0
23	11458.5
24	14214.5
25	18257.0
26	23797.0
27	30168.5
28	36609.5
29	43921.5
30	51341.0
31	58878.5
32	68048.0
33	77627.5
34	86201.5
35	94748.5
36	104721.0
37	113525.0
38	120577.5
39	126686.0
40	132670.5
41	138169.0
42	142335.5
43	146554.0
44	155050.5
45	167132.5
46	168862.5
47	165314.5
48	163748.0
49	160095.5
50	156790.0
51	153406.0
52	147838.0
53	140341.5
54	131324.0
55	121816.5
56	111065.0
57	99193.0
58	88276.5
59	76507.0
60	64121.0
61	51834.5
62	40941.0
63	31652.0
64	23733.0
65	17789.5
66	13194.0
67	10004.5
68	7724.0
69	5766.5
70	4194.0
71	2909.5
72	1954.0
73	1274.5
74	785.5
75	489.5
76	324.5
77	200.0
78	134.5
79	92.5
80	61.0
81	40.0
82	25.0
83	17.0
84	12.0
85	9.0
86	5.5
87	3.0
88	2.0
89	2.0
90	2.0
91	2.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006839872587616452
3	8.175146519063488E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	9.537794395946274E-5
22-23	0.0
24-25	2.0440470332496957E-4
26-27	2.316843314612331E-4
28-29	0.00257670784263977
30-31	0.0035075522239723586
32-33	0.002681448562292102
34-35	7.823420190682829E-4
36-37	0.0
38-39	1.3861437467105077E-5
40-41	1.8126156936824485E-4
42-43	0.0027537263818789067
44-45	2.551334262774956E-4
46-47	5.870392332637621E-4
48-49	0.001446115286045942
50-51	0.0012700626097071324
52-53	0.0
54-55	0.0033839792460992313
56-57	0.054614772372488574
58-59	0.07101167586235116
60-61	0.047828763275547755
62-63	0.008622711326542973
64-65	0.0
66-67	0.0
68-69	1.852509092346189E-4
70-71	0.00173753786188922
72-73	0.008162023470422509
74-75	0.013402594517584446
76-77	0.014570033918325061
78-79	0.0043652077190657335
80-81	0.00797145422242948
82-83	0.047317583657689545
84-85	0.04011579038046795
86-87	0.009931425056401216
88-89	0.0025729617188496915
90-91	0.0034497628288055193
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	24.0
20-21	117.0
22-23	276.0
24-25	317.0
26-27	956.0
28-29	2567.0
30-31	8158.0
32-33	11065.0
34-35	14829.0
36-37	23797.0
38-39	14790.0
40-41	26863.0
42-43	29857.0
44-45	34901.0
46-47	35191.0
48-49	33086.0
50-51	16954.0
52-53	2269.0
54-55	1994.0
56-57	1890.0
58-59	17426.0
60-61	32810.0
62-63	32598.0
64-65	36841.0
66-67	40569.0
68-69	41966.0
70-71	50008.0
72-73	34292.0
74-75	33151.0
76-77	34339.0
78-79	35945.0
80-81	35698.0
82-83	33278.0
84-85	32634.0
86-87	33450.0
88-89	35010.0
90-91	35228.0
92-93	34873.0
94-95	39557.0
96-97	127119.0
98-99	872961.0
100-101	1740005.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.06562046022147
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.72244999305637	83.51104989216479
2	5.535734196496465	9.971586702206391
3	1.0605531799311316	2.865581405446748
4	0.34025261596758677	1.2258025188133663
5	0.13690875573213684	0.6165386015725903
6	0.0709325138751374	0.38331485237805096
7	0.039796875676690935	0.25090312101396256
8	0.022638583093272855	0.16311664261087197
9	0.017230166089550823	0.13966610395392562
>10	0.051844455847828354	0.7054295400086298
>50	0.001055517124602616	0.06480909261389317
>100	6.031471092540296E-4	0.10220152721673526
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.7250488396878294E-5	0.0
5	0.0	0.0	0.0	2.7250488396878294E-5	0.0
6	2.7250488396878294E-5	0.0	0.0	2.7250488396878294E-5	0.0
7	2.7250488396878294E-5	0.0	0.0	2.7250488396878294E-5	0.0
8	2.7250488396878294E-5	2.7250488396878294E-5	0.0	5.450097679375659E-5	0.0
9	2.7250488396878294E-5	2.7250488396878296E-4	0.0	5.450097679375659E-5	0.0
10-11	2.7250488396878294E-5	2.7250488396878296E-4	0.0	6.812622099219574E-5	0.0
12-13	2.7250488396878294E-5	2.7250488396878296E-4	0.0	8.175146519063488E-5	0.0
14-15	5.450097679375659E-5	3.542563491594178E-4	0.0	8.175146519063488E-5	2.7250488396878294E-5
16-17	8.175146519063488E-5	3.542563491594178E-4	0.0	8.175146519063488E-5	2.7250488396878294E-5
18-19	8.175146519063488E-5	3.6788159335785696E-4	0.0	8.175146519063488E-5	8.175146519063488E-5
20-21	8.175146519063488E-5	4.4963305854849186E-4	0.0	8.175146519063488E-5	8.175146519063488E-5
22-23	9.537670938907403E-5	5.041340353422485E-4	0.0	9.537670938907403E-5	8.175146519063488E-5
24-25	1.0900195358751317E-4	5.177592795406876E-4	0.0	1.771281745797089E-4	1.0900195358751317E-4
26-27	1.0900195358751317E-4	5.177592795406876E-4	0.0	2.4525439557190464E-4	1.0900195358751317E-4
28-29	1.0900195358751317E-4	5.450097679375659E-4	0.0	6.267612331282008E-4	1.0900195358751317E-4
30-31	1.0900195358751317E-4	6.540117215250791E-4	0.0	0.0023435420021315333	1.0900195358751317E-4
32-33	1.0900195358751317E-4	6.540117215250791E-4	0.0	0.005627225853955368	1.3625244198439148E-4
34-35	1.0900195358751317E-4	6.948874541203965E-4	0.0	0.01005543021844809	1.6350293038126977E-4
36-37	1.3625244198439148E-4	7.357631867157139E-4	0.0	0.020778497402619698	1.6350293038126977E-4
38-39	1.3625244198439148E-4	7.357631867157139E-4	0.0	0.21564673992869637	1.6350293038126977E-4
40-41	1.3625244198439148E-4	7.630136751125922E-4	0.0	0.41610133257613313	1.6350293038126977E-4
42-43	1.3625244198439148E-4	7.766389193110314E-4	0.0	0.4389372418527171	1.6350293038126977E-4
44-45	1.3625244198439148E-4	8.720156287001054E-4	0.0	0.4689400295776801	1.6350293038126977E-4
46-47	1.3625244198439148E-4	9.810175822876186E-4	0.0	0.5015724894329419	1.771281745797089E-4
48-49	1.3625244198439148E-4	9.810175822876186E-4	0.0	0.5342321997766004	1.9075341877814806E-4
50-51	1.6350293038126977E-4	0.00111727002427201	0.0	0.567382418911403	1.9075341877814806E-4
52-53	1.9075341877814806E-4	0.00111727002427201	0.0	1.1486625869052138	1.9075341877814806E-4
54-55	1.9075341877814806E-4	0.00111727002427201	0.0	1.9137336738917705	1.9075341877814806E-4
56-57	1.9075341877814806E-4	0.00111727002427201	0.0	2.747721246034032	1.9075341877814806E-4
58-59	1.9075341877814806E-4	0.0011308952684704492	0.0	3.614082398391785	1.9075341877814806E-4
60-61	1.9075341877814806E-4	0.0011717710010657667	0.0	3.8417874794361	1.9075341877814806E-4
62-63	2.1800390717502635E-4	0.0011717710010657667	0.0	3.8498263735131792	2.1800390717502635E-4
64-65	2.1800390717502635E-4	0.001389774908240793	0.0	3.8572793820897253	2.1800390717502635E-4
66-67	2.1800390717502635E-4	0.001389774908240793	0.0	3.8643236333403186	2.1800390717502635E-4
68-69	2.1800390717502635E-4	0.0014579011292329886	0.0	3.8703459912760287	2.1800390717502635E-4
70-71	2.4525439557190464E-4	0.001498776861828306	0.0	3.8754554578504434	2.1800390717502635E-4
72-73	2.7250488396878296E-4	0.0015260273502251845	0.0	3.880619425401652	2.1800390717502635E-4
74-75	2.7250488396878296E-4	0.0015669030828205019	0.0	3.884597996707596	2.1800390717502635E-4
76-77	2.7250488396878296E-4	0.0016350293038126977	0.0	3.888072433978198	2.1800390717502635E-4
78-79	2.7250488396878296E-4	0.001662279792209576	0.0	3.8907021061084963	2.1800390717502635E-4
80-81	2.7250488396878296E-4	0.001662279792209576	0.0	3.891451494539411	2.3162915137346548E-4
82-83	2.7250488396878296E-4	0.0016759050364080152	0.0	3.8921872577261265	2.8613012816722206E-4
84-85	2.7250488396878296E-4	0.0017304060132017716	0.0	3.892473387854294	2.997553723656612E-4
86-87	2.7250488396878296E-4	0.0018257827225908456	0.0	3.892568764563683	3.133806165641004E-4
88	2.7250488396878296E-4	0.0018257827225908456	0.0	3.892568764563683	3.2700586076253954E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5135	0.0	48.954136	1
GTATCAA	9710	0.0	37.867737	1
ATCTCGT	7835	0.0	33.282288	94
TCAACGC	11470	0.0	31.201107	4
ATCAACG	11645	0.0	30.768806	3
TATCAAC	11785	0.0	30.730326	2
CAACGCA	11815	0.0	30.29003	5
AACGCAG	12110	0.0	29.587345	6
ACGCAGA	13725	0.0	26.105848	7
TATCTCG	8720	0.0	25.779688	94
CGCAGAG	14280	0.0	25.091228	8
TGGTATC	2600	0.0	24.744656	2
GTGGTAT	2950	0.0	24.40858	1
GCAGAGT	16340	0.0	21.797586	9
GTACATG	13090	0.0	21.547464	1
TACATGG	13200	0.0	20.690067	2
ACATGGG	13075	0.0	20.528301	3
GAGTACT	8495	0.0	19.910458	12-13
CAGAGTA	15950	0.0	18.697844	10-11
AGAGTAC	14645	0.0	18.138494	10-11
>>END_MODULE