FastQCFastQC Report
Fri 27 May 2016
SRR2139473_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139473_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3652537
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49950.051.5222131
GTATCAA97400.038.5434571
TCAACGC110850.033.1347924
ATCAACG112100.032.7653163
TATCAAC114750.032.197632
CAACGCA114950.031.9529535
AACGCAG118250.031.0971536
ACGCAGA136400.026.9280917
GTGGTAT25400.026.5840261
CGCAGAG140950.026.058838
TGGTATC24900.025.4123172
GCAGAGT162150.022.5470689
GTACATG122600.021.0259481
GAGTACT92000.020.81577312-13
TACATGG124200.020.0712262
ACATGGG121700.020.0623193
CAGAGTA159250.019.55800210-11
GTACTTT102850.018.4963714-15
GTATAGG11400.018.2536031
AGAGTAC146700.018.20641110-11
Produced by FastQC (version 0.11.5)