##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139473_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3652537
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.647358260847188	28.0	18.0	33.0	18.0	33.0
2	30.55183972126771	32.0	27.0	33.0	27.0	33.0
3	28.79770006436622	31.0	28.0	33.0	18.0	33.0
4	31.96291810322524	33.0	32.0	33.0	31.0	33.0
5	32.71764009509007	33.0	33.0	33.0	32.0	34.0
6	37.04269279133928	38.0	37.0	38.0	36.0	38.0
7	37.023670944332665	38.0	38.0	38.0	35.0	38.0
8	37.32371992398708	38.0	38.0	38.0	36.0	38.0
9	37.435446102257146	38.0	38.0	38.0	37.0	38.0
10-11	37.403525960175074	38.0	38.0	38.0	37.0	38.0
12-13	37.51924018839508	38.0	38.0	38.0	37.5	38.0
14-15	37.49663370966536	38.0	38.0	38.0	37.0	38.0
16-17	37.544997764567476	38.0	38.0	38.0	37.5	38.0
18-19	37.533563246587235	38.0	38.0	38.0	37.5	38.0
20-21	37.54136807432761	38.0	38.0	38.0	38.0	38.0
22-23	37.54876432577608	38.0	38.0	38.0	38.0	38.0
24-25	37.55575584807606	38.0	38.0	38.0	38.0	38.0
26-27	37.51969311660153	38.0	38.0	38.0	37.5	38.0
28-29	37.491900347877504	38.0	38.0	38.0	37.0	38.0
30-31	37.50050412554576	38.0	38.0	38.0	38.0	38.0
32-33	37.48945304553447	38.0	38.0	38.0	37.5	38.0
34-35	37.38530155895526	38.0	38.0	38.0	37.0	38.0
36-37	37.42636388061981	38.0	38.0	38.0	37.0	38.0
38-39	37.43630144329199	38.0	38.0	38.0	37.0	38.0
40-41	37.432049626397465	38.0	38.0	38.0	37.0	38.0
42-43	37.39299382048668	38.0	38.0	38.0	37.0	38.0
44-45	37.36170796741804	38.0	38.0	38.0	37.0	38.0
46-47	37.32651632479775	38.0	38.0	38.0	37.0	38.0
48-49	37.264172787708304	38.0	38.0	38.0	37.0	38.0
50-51	37.23349423849237	38.0	38.0	38.0	37.0	38.0
52-53	37.21748612951177	38.0	38.0	38.0	37.0	38.0
54-55	37.19304067319911	38.0	38.0	38.0	37.0	38.0
56-57	37.14979964598005	38.0	38.0	38.0	37.0	38.0
58-59	37.1485653481828	38.0	38.0	38.0	37.0	38.0
60-61	37.1519369185165	38.0	38.0	38.0	36.5	38.0
62-63	37.16466791798625	38.0	38.0	38.0	36.5	38.0
64-65	37.17429614453662	38.0	38.0	38.0	37.0	38.0
66-67	37.1753139277874	38.0	38.0	38.0	36.0	38.0
68-69	37.169675694413016	38.0	38.0	38.0	36.0	38.0
70-71	37.16665914244716	38.0	38.0	38.0	36.0	38.0
72-73	37.15796348570419	38.0	38.0	38.0	36.0	38.0
74-75	37.16018047799842	38.0	38.0	38.0	36.0	38.0
76-77	37.1461420097352	38.0	38.0	38.0	36.0	38.0
78-79	37.13678612106186	38.0	38.0	38.0	36.0	38.0
80-81	37.12788993820591	38.0	38.0	38.0	36.0	38.0
82-83	37.082975968742495	38.0	38.0	38.0	36.0	38.0
84-85	37.07552683380787	38.0	38.0	38.0	36.0	38.0
86-87	37.06958408584792	38.0	38.0	38.0	36.0	38.0
88-89	37.05455444299466	38.0	38.0	38.0	36.0	38.0
90-91	37.04930842376915	38.0	38.0	38.0	36.0	38.0
92-93	37.03618359205538	38.0	38.0	38.0	36.0	38.0
94-95	37.019396765086086	38.0	38.0	38.0	36.0	38.0
96-97	37.007238828990964	38.0	38.0	38.0	36.0	38.0
98-99	37.027156468977864	38.0	38.0	38.0	36.0	38.0
100	35.68486207820032	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	5.0
21	38.0
22	139.0
23	493.0
24	1239.0
25	2552.0
26	4719.0
27	8091.0
28	12901.0
29	19052.0
30	25974.0
31	33627.0
32	43043.0
33	56492.0
34	84596.0
35	161777.0
36	569746.0
37	2628052.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.37080007676856	18.020488225033723	12.128665637062678	22.48004606113504
2	14.725158342396332	20.130622209225532	42.20371106606079	22.940508382317347
3	19.19630720202381	25.566821765276803	29.956427283666187	25.2804437490332
4	12.947083082252146	17.672209754480242	39.153169427168024	30.227537736099592
5	13.754056427080682	37.47354236247299	34.984724316276605	13.787676894169723
6	30.113781188253537	37.383276336420415	18.830062501762473	13.672879973563582
7	26.924463735754077	32.756711294095034	22.781398244562613	17.537426725588272
8	24.250952146412207	36.941610721534104	20.943086955724198	17.864350176329495
9	25.75519426634145	17.63437303988981	21.53544235143956	35.07499034232918
10-11	24.41500250373918	26.955264245098682	28.468240020566526	20.16149323059561
12-13	25.351105272855552	24.476918372079464	28.787442810298703	21.38453354476628
14-15	22.475254870792547	25.83407094849416	26.633953331615807	25.05672084909749
16-17	21.858806632212076	28.955257674323352	27.33067454210594	21.855261151358633
18-19	21.858697119289964	27.95244784652421	29.264795948678955	20.92405908550687
20-21	22.683462112778706	27.028748544147046	29.262630670844995	21.025158672229253
22-23	22.4928484048	26.845683798734616	29.113618367662085	21.547849428803296
24-25	22.174068227038656	27.035274509033474	29.190033067670228	21.60062419625764
26-27	22.03532765935772	27.153845542951487	29.204374578785497	21.606452218905297
28-29	22.00523199998466	27.149543456705878	29.268011726320296	21.577212816989167
30-31	22.245880150095157	27.045590807391456	29.145354537356727	21.56317450515666
32-33	21.993984692580035	27.28935673086686	29.03726091516152	21.679397661391583
34-35	22.185524163361865	27.277790835024884	29.047683076457638	21.489001925155616
36-37	22.186133621525663	27.150063927456102	29.011748762469036	21.6520536885492
38-39	22.020017172528796	27.319784136664264	29.09336480377623	21.566833887030704
40-41	22.282472469511795	27.142454627953633	28.95362357786991	21.621449324664656
42-43	22.113435138257902	27.265907660111278	28.77505279525316	21.845604406377664
44-45	22.188528720570204	27.34155854792146	28.713617274429375	21.756295457078963
46-47	22.29486808228036	27.36300024308827	28.44603454723446	21.89609712739691
48-49	22.23354435366142	27.480917799394746	28.372883231298978	21.912654615644854
50-51	22.191611464325273	27.64171887463617	28.26032689484949	21.90634276618907
52-53	22.363139971325175	27.593418007619242	28.108363814378713	21.93507820667687
54-55	22.3490030930182	27.47828176201928	28.176602366592736	21.996112778369785
56-57	22.249222333952552	27.598086748546617	28.19297846170969	21.959712455791138
58-59	22.277302437166195	27.549966102677192	28.204866605130295	21.967864855026313
60-61	22.319463242559372	27.545966783033133	28.28239445947448	21.85217551493302
62-63	22.253242605875993	27.62964282204972	28.256869042939964	21.86024552913432
64-65	22.257060331276087	27.557214431185486	28.243384932281216	21.942340305257208
66-67	22.329969749159233	27.618637744501314	28.06130895423949	21.990083552099964
68-69	22.206887514869642	27.654823841628364	28.179703404447114	21.95858523905488
70-71	22.25853066279703	27.572124750273154	28.19555077538721	21.973793811542606
72-73	22.417319647539315	27.644667161358498	27.98859449158978	21.949418699512403
74-75	22.09476964545779	27.734535326406878	28.065778321845258	22.104916706290073
76-77	22.368042467626058	27.45907643029674	28.00989168285391	22.16298941922329
78-79	22.303938079718648	27.58456081340058	27.91292493619382	22.198576170686955
80-81	22.330027231065554	27.6259294219621	27.855219824269394	22.188823522702954
82-83	22.39422378606806	27.532837625628943	27.931977793027368	22.140960795275635
84-85	22.43100562888026	27.548460637912363	27.891197587603173	22.129336145604206
86-87	22.341431831882517	27.581372634805984	27.95676704276432	22.120428490547177
88-89	22.37979030317796	27.59580256808785	27.890243597650972	22.134163531083217
90-91	22.434934353343007	27.595313530851517	27.873332500330932	22.096419615474545
92-93	22.49729640837908	27.593025895492108	27.834892330022292	22.074785366106525
94-95	22.388895257816817	27.664230039408196	27.875320822187206	22.071553880587786
96-97	22.496676774905357	27.71555949147077	27.9194268343881	21.868336899235775
98-99	23.476026031566903	29.619815480774914	28.154518086272716	18.749640401385463
100	29.942699964292093	40.53215943581503	29.525140599892875	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	587.0
1	555.0
2	709.0
3	998.0
4	1287.5
5	1811.5
6	2332.0
7	2963.0
8	3999.0
9	4760.0
10	4780.0
11	4716.0
12	5173.0
13	6277.0
14	8412.5
15	10887.0
16	13028.5
17	14791.0
18	15235.5
19	14304.0
20	12938.5
21	11785.0
22	11564.5
23	13067.0
24	16205.5
25	20937.0
26	27159.0
27	34245.5
28	41763.0
29	50473.0
30	59473.0
31	67805.5
32	77167.5
33	87375.0
34	96198.5
35	104770.0
36	114832.5
37	123289.0
38	129609.5
39	134823.5
40	140122.0
41	144564.0
42	146901.5
43	149231.5
44	151972.0
45	154341.5
46	154943.0
47	154476.5
48	153314.0
49	151232.0
50	148819.5
51	144768.5
52	138626.5
53	131349.0
54	122690.5
55	113680.5
56	103908.5
57	93567.5
58	83871.5
59	72411.5
60	60248.0
61	49132.0
62	39066.0
63	30399.0
64	23017.0
65	17076.5
66	12572.5
67	9589.0
68	7359.5
69	5523.0
70	4091.5
71	2924.0
72	2018.0
73	1323.0
74	837.0
75	541.0
76	354.0
77	215.0
78	142.5
79	99.5
80	67.0
81	44.0
82	34.0
83	26.5
84	17.0
85	10.5
86	9.0
87	10.0
88	5.5
89	1.5
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.008897924921773551
3	6.023210716277481E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	5.4757015742642024E-5
22-23	0.0
24-25	1.505998529050164E-4
26-27	3.01233358910784E-4
28-29	0.002259962531190907
30-31	0.0033589168548802594
32-33	0.0022394434969171245
34-35	6.061709859563959E-4
36-37	0.0
38-39	1.3918403637045892E-5
40-41	1.9615307393583805E-4
42-43	0.0024825953842063206
44-45	2.2749815726492618E-4
46-47	6.313941210893385E-4
48-49	0.0011296257086591023
50-51	0.0011100962818639628
52-53	0.0
54-55	0.0033368778391268945
56-57	0.055110076621845
58-59	0.07116991179244266
60-61	0.04595707122585161
62-63	0.007967700569207002
64-65	0.0
66-67	0.0
68-69	1.578725100545055E-4
70-71	0.001403410510811125
72-73	0.007972203262515474
74-75	0.01260093142833265
76-77	0.014219981471331303
78-79	0.004496345433480054
80-81	0.007345608885570507
82-83	0.047167812671074415
84-85	0.03976721712506816
86-87	0.008905053626578768
88-89	0.0023943405479299787
90-91	0.0027569616374090006
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	17.0
20-21	117.0
22-23	301.0
24-25	306.0
26-27	831.0
28-29	2283.0
30-31	7224.0
32-33	9227.0
34-35	13065.0
36-37	20947.0
38-39	23787.0
40-41	23555.0
42-43	26742.0
44-45	31456.0
46-47	32444.0
48-49	30051.0
50-51	27058.0
52-53	25128.0
54-55	27222.0
56-57	28013.0
58-59	29666.0
60-61	29103.0
62-63	28365.0
64-65	29439.0
66-67	31207.0
68-69	31781.0
70-71	31290.0
72-73	29430.0
74-75	29703.0
76-77	31164.0
78-79	32131.0
80-81	31835.0
82-83	29640.0
84-85	29003.0
86-87	30274.0
88-89	31458.0
90-91	32189.0
92-93	31525.0
94-95	36280.0
96-97	110041.0
98-99	834919.0
100-101	1792320.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.97357142685195
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.51486991610271	85.07381697785338
2	4.9333031376360434	8.97600410724091
3	0.9365247839356577	2.5559701297316297
4	0.30500781958718987	1.1099060264385439
5	0.12009842339226481	0.5462891249364255
6	0.06506499129655181	0.35515167798626146
7	0.035064604365276725	0.2232966602845165
8	0.02102231471480408	0.15299800394119917
9	0.015100111845418096	0.12363399931703367
>10	0.052419432989434626	0.7408145641769512
>50	0.0010092128990754287	0.05909491867542761
>100	5.152512355814737E-4	0.08302380941769859
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.7378230528534004E-5	0.0
3	0.0	0.0	0.0	5.475646105706801E-5	0.0
4	0.0	8.2134691585602E-5	0.0	5.475646105706801E-5	0.0
5	0.0	8.2134691585602E-5	0.0	5.475646105706801E-5	0.0
6	5.475646105706801E-5	8.2134691585602E-5	0.0	5.475646105706801E-5	0.0
7	5.475646105706801E-5	8.2134691585602E-5	0.0	5.475646105706801E-5	0.0
8	8.2134691585602E-5	1.3689115264267E-4	0.0	5.475646105706801E-5	0.0
9	8.2134691585602E-5	2.1902584422827203E-4	0.0	5.475646105706801E-5	0.0
10-11	8.2134691585602E-5	2.1902584422827203E-4	0.0	1.09512922114136E-4	0.0
12-13	8.2134691585602E-5	2.1902584422827203E-4	0.0	1.3689115264267E-4	0.0
14-15	8.2134691585602E-5	3.14849651078141E-4	0.0	1.5058026790693702E-4	2.7378230528534004E-5
16-17	8.2134691585602E-5	3.28538766342408E-4	0.0	1.64269383171204E-4	5.475646105706801E-5
18-19	9.582380684986902E-5	3.8329522739947606E-4	0.0	1.7795849843547102E-4	5.475646105706801E-5
20-21	1.0951292211413602E-4	4.3805168845654406E-4	0.0	1.9164761369973803E-4	5.475646105706801E-5
22-23	1.0951292211413602E-4	4.791190342493451E-4	0.0	2.1902584422827203E-4	6.8445576321335E-5
24-25	1.0951292211413602E-4	5.20186380042146E-4	0.0	2.7378230528534E-4	1.2320203737840302E-4
26-27	1.0951292211413602E-4	5.4756461057068E-4	0.0	2.8747142054960705E-4	1.64269383171204E-4
28-29	1.0951292211413602E-4	5.4756461057068E-4	0.0	5.33875495306413E-4	1.7795849843547102E-4
30-31	1.0951292211413602E-4	6.57077532684816E-4	0.0	0.0017932740996189772	1.9164761369973803E-4
32-33	1.0951292211413602E-4	6.57077532684816E-4	0.0	0.004681677420379315	1.9164761369973803E-4
34-35	1.0951292211413602E-4	7.939686853274861E-4	0.0	0.0081724018127674	2.1902584422827203E-4
36-37	1.0951292211413602E-4	9.445489532344231E-4	0.0	0.013962897569552341	2.1902584422827203E-4
38-39	1.0951292211413602E-4	0.001012994529555758	0.0	0.030006540659273266	2.1902584422827203E-4
40-41	1.0951292211413602E-4	0.001122507451669894	0.0	0.04765181023491343	2.1902584422827203E-4
42-43	1.0951292211413602E-4	0.001163574797462695	0.0	0.05727525826569313	2.3271495949253905E-4
44-45	1.0951292211413602E-4	0.00123202037378403	0.0	0.0683634416297494	2.4640407475680603E-4
46-47	1.0951292211413602E-4	0.0013278441806338992	0.0	0.08091636032708224	2.7378230528534006E-4
48-49	1.0951292211413602E-4	0.0013415332958981662	0.0	0.09312705114280842	3.28538766342408E-4
50-51	1.3689115264267E-4	0.0013689115264267002	0.0	0.1051597834600991	3.28538766342408E-4
52-53	1.3689115264267E-4	0.0013689115264267002	0.0	0.14077886137772183	3.42227881606675E-4
54-55	1.3689115264267E-4	0.0013689115264267002	0.0	0.18633623697720242	3.5591699687094203E-4
56-57	1.3689115264267E-4	0.0013962897569552342	0.0	0.2502644052613293	3.5591699687094203E-4
58-59	1.3689115264267E-4	0.0014510462180123021	0.0	0.28960692253083264	3.696061121352091E-4
60-61	1.64269383171204E-4	0.0014510462180123021	0.0	0.30495242074207596	3.9698434266374305E-4
62-63	1.64269383171204E-4	0.0014510462180123021	0.0	0.31732738094097335	4.3805168845654406E-4
64-65	1.64269383171204E-4	0.001478424448540836	0.0	0.32347379369462925	4.5174080372081105E-4
66-67	1.64269383171204E-4	0.001478424448540836	0.0	0.3294696261803782	4.6542991898507804E-4
68-69	1.64269383171204E-4	0.0015605591401264382	0.0	0.33608146885301915	4.6542991898507804E-4
70-71	1.64269383171204E-4	0.0015879373706549722	0.0	0.34140653469081905	4.6542991898507804E-4
72-73	1.9164761369973803E-4	0.0015879373706549722	0.0	0.34554064750062763	4.928081495136121E-4
74-75	1.9164761369973803E-4	0.0016016264859192392	0.0	0.3487712787029946	4.928081495136121E-4
76-77	2.0533672896400502E-4	0.0016563829469763071	0.0	0.3520019099053616	4.928081495136121E-4
78-79	2.1902584422827203E-4	0.001697450292769108	0.0	0.35405527719500174	4.928081495136121E-4
80-81	2.1902584422827203E-4	0.001697450292769108	0.0	0.3547397329582151	5.06497264777879E-4
82-83	2.1902584422827203E-4	0.001724828523297642	0.0	0.35524623022299295	5.20186380042146E-4
84-85	2.1902584422827203E-4	0.001724828523297642	0.0	0.355506323413014	5.20186380042146E-4
86-87	2.1902584422827203E-4	0.001724828523297642	0.0	0.3555747689893354	5.33875495306413E-4
88	2.1902584422827203E-4	0.0017522067538261763	0.0	0.3555884581045996	5.4756461057068E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4995	0.0	51.522213	1
GTATCAA	9740	0.0	38.543457	1
TCAACGC	11085	0.0	33.134792	4
ATCAACG	11210	0.0	32.765316	3
TATCAAC	11475	0.0	32.19763	2
CAACGCA	11495	0.0	31.952953	5
AACGCAG	11825	0.0	31.097153	6
ACGCAGA	13640	0.0	26.928091	7
GTGGTAT	2540	0.0	26.584026	1
CGCAGAG	14095	0.0	26.05883	8
TGGTATC	2490	0.0	25.412317	2
GCAGAGT	16215	0.0	22.547068	9
GTACATG	12260	0.0	21.025948	1
GAGTACT	9200	0.0	20.815773	12-13
TACATGG	12420	0.0	20.071226	2
ACATGGG	12170	0.0	20.062319	3
CAGAGTA	15925	0.0	19.558002	10-11
GTACTTT	10285	0.0	18.49637	14-15
GTATAGG	1140	0.0	18.253603	1
AGAGTAC	14670	0.0	18.206411	10-11
>>END_MODULE