FastQCFastQC Report
Fri 27 May 2016
SRR2139472_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139472_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2634538
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34650.044.89741
GTATCAA61400.036.391971
TCAACGC68600.031.5836934
ATCAACG69200.031.3104383
TATCAAC71250.030.7100522
CAACGCA71000.030.5160715
AACGCAG73750.029.435346
ACGCAGA84150.025.8475537
CGCAGAG86200.025.232858
TGGTATC17550.023.7824272
GTGGTAT20050.022.0786741
GCAGAGT102100.021.3859189
GAGTACT53450.019.12440312-13
GTACATG83850.018.8047081
TACATGG83750.018.7264822
CAGAGTA98850.018.65628810-11
GTACTTT58250.017.51230414-15
ACATGGG85300.017.444473
AGAGTAC91400.017.31759610-11
CATGGGG59500.016.3651084
Produced by FastQC (version 0.11.5)