##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139472_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2634538
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.818579576381133	28.0	18.0	33.0	18.0	33.0
2	30.754895545253095	32.0	29.0	33.0	27.0	33.0
3	28.877802483775145	31.0	28.0	33.0	18.0	33.0
4	32.009342055419204	33.0	32.0	33.0	31.0	33.0
5	32.74881819886447	33.0	33.0	33.0	32.0	34.0
6	37.102352670563114	38.0	38.0	38.0	36.0	38.0
7	37.09987443718785	38.0	38.0	38.0	36.0	38.0
8	37.396324896433455	38.0	38.0	38.0	37.0	38.0
9	37.50248051081442	38.0	38.0	38.0	37.0	38.0
10-11	37.46826198749079	38.0	38.0	38.0	37.0	38.0
12-13	37.57357248215816	38.0	38.0	38.0	38.0	38.0
14-15	37.55011182226258	38.0	38.0	38.0	37.5	38.0
16-17	37.59305103968893	38.0	38.0	38.0	38.0	38.0
18-19	37.583785278481464	38.0	38.0	38.0	38.0	38.0
20-21	37.588343537578474	38.0	38.0	38.0	38.0	38.0
22-23	37.596306510812994	38.0	38.0	38.0	38.0	38.0
24-25	37.601421620302304	38.0	38.0	38.0	38.0	38.0
26-27	37.56674861966407	38.0	38.0	38.0	38.0	38.0
28-29	37.54420375590041	38.0	38.0	38.0	38.0	38.0
30-31	37.55056995447249	38.0	38.0	38.0	38.0	38.0
32-33	37.54211251078117	38.0	38.0	38.0	38.0	38.0
34-35	37.43705661431473	38.0	38.0	38.0	37.0	38.0
36-37	37.487029391191626	38.0	38.0	38.0	37.0	38.0
38-39	37.49326577759062	38.0	38.0	38.0	38.0	38.0
40-41	37.492814440858524	38.0	38.0	38.0	38.0	38.0
42-43	37.464576097705105	38.0	38.0	38.0	38.0	38.0
44-45	37.43742215494024	38.0	38.0	38.0	37.5	38.0
46-47	37.414303933512876	38.0	38.0	38.0	38.0	38.0
48-49	37.36057932003326	38.0	38.0	38.0	37.0	38.0
50-51	37.335317681379806	38.0	38.0	38.0	37.0	38.0
52-53	37.317444397795114	38.0	38.0	38.0	37.0	38.0
54-55	37.29136334841276	38.0	38.0	38.0	37.0	38.0
56-57	37.24813499403072	38.0	38.0	38.0	37.0	38.0
58-59	37.24410971398288	38.0	38.0	38.0	37.0	38.0
60-61	37.24161571261381	38.0	38.0	38.0	37.0	38.0
62-63	37.24650430291288	38.0	38.0	38.0	37.0	38.0
64-65	37.25643516360974	38.0	38.0	38.0	37.0	38.0
66-67	37.253070386415686	38.0	38.0	38.0	37.0	38.0
68-69	37.24603796214513	38.0	38.0	38.0	37.0	38.0
70-71	37.24391499030544	38.0	38.0	38.0	37.0	38.0
72-73	37.230769046523136	38.0	38.0	38.0	37.0	38.0
74-75	37.230694574870384	38.0	38.0	38.0	37.0	38.0
76-77	37.21861326176923	38.0	38.0	38.0	36.5	38.0
78-79	37.20843901423721	38.0	38.0	38.0	36.0	38.0
80-81	37.20236899097641	38.0	38.0	38.0	36.0	38.0
82-83	37.153929998368554	38.0	38.0	38.0	36.0	38.0
84-85	37.14594471688879	38.0	38.0	38.0	36.0	38.0
86-87	37.13675499844773	38.0	38.0	38.0	36.0	38.0
88-89	37.124872589957434	38.0	38.0	38.0	36.0	38.0
90-91	37.11818076096572	38.0	38.0	38.0	36.0	38.0
92-93	37.10245941231568	38.0	38.0	38.0	36.0	38.0
94-95	37.08460708013408	38.0	38.0	38.0	36.0	38.0
96-97	37.07264915186653	38.0	38.0	38.0	36.0	38.0
98-99	37.08340026645719	38.0	38.0	38.0	36.0	38.0
100	35.737634553046405	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	6.0
21	25.0
22	98.0
23	338.0
24	832.0
25	1636.0
26	3020.0
27	4960.0
28	8029.0
29	11818.0
30	15887.0
31	20446.0
32	26180.0
33	35156.0
34	53537.0
35	105744.0
36	399096.0
37	1947728.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.23448209894866	18.558130495745363	12.81340409589841	22.39398330940757
2	14.761765210538544	20.54962016359614	42.211038108152025	22.4775765177133
3	19.38056174265026	25.83134814895791	30.147169347234936	24.640920761156888
4	13.125489174952119	18.058688088765468	39.559991163536075	29.255831572746345
5	14.106230390299931	37.86728451060489	34.89283510049959	13.133649998595578
6	30.330706939888515	37.871004327893544	18.81430444351154	12.983984288706408
7	27.351247163639318	33.05095618282978	22.758411531737252	16.83938512179365
8	24.63312353057728	37.269646518668544	21.00425197890484	17.092977971849333
9	26.242020422556063	18.03124494693187	21.52255917356288	34.20417545694919
10-11	24.7352097407591	27.2930775718551	28.56574852972324	19.40596415766256
12-13	25.624094243468875	24.74029602154154	28.750676589216024	20.884933145773566
14-15	22.754900479704602	26.258057389948448	26.478968988111006	24.508073142235943
16-17	22.217310966856427	29.32233279611074	27.175296010154344	21.28506022687849
18-19	22.187837108441784	28.428760564470885	28.828640923000542	20.55476140408679
20-21	23.03251987464851	27.48829764297904	28.76842086883643	20.710761613536015
22-23	22.88758925592322	27.348838586420765	28.614696447300147	21.14887571035587
24-25	22.57817151809632	27.549484696344102	28.640071732713647	21.232272052845932
26-27	22.4904011052824	27.71826309077386	28.62604346335487	21.165292340588866
28-29	22.464102089356672	27.73305987969939	28.626702557456124	21.176135473487815
30-31	22.701047659944788	27.60240741469431	28.56676857556228	21.129776349798625
32-33	22.46751663224636	27.777185054221505	28.51184261665435	21.243455696877785
34-35	22.654850211894807	27.848914673240223	28.46471754142023	21.031517573444734
36-37	22.680624481912744	27.630309440718147	28.457214605535874	21.23185147183323
38-39	22.511215423257255	27.833994155093833	28.55911104259146	21.095679379057447
40-41	22.742720170281817	27.656105445535406	28.463115172728447	21.138059211454333
42-43	22.62474578078424	27.790452804212396	28.27335171081496	21.311449704188405
44-45	22.63089415913723	27.903572077468	28.199872796501886	21.265660966892888
46-47	22.758593435649825	27.868410421975693	27.97333352493725	21.399662617437233
48-49	22.7028802074257	27.915921675442036	27.898253421587306	21.482944695544962
50-51	22.67956704783216	28.115094181958728	27.80951389556717	21.39582487464194
52-53	22.87350141153959	27.998668308594066	27.739074585173825	21.388755694692524
54-55	22.87896555330192	27.85787890148526	27.787631396315387	21.475524148897428
56-57	22.805452389650615	28.092537817279055	27.68754977330392	21.414460019766405
58-59	22.887670726124675	28.07727302507744	27.734625247821874	21.300431000976015
60-61	23.01155779044068	28.141499472016896	27.623808104189802	21.22313463335263
62-63	22.97773483125428	28.287410095022402	27.576174464743904	21.158680608979413
64-65	23.11566337382478	28.178145021567623	27.516412741857017	21.18977886275058
66-67	23.461025477189562	28.356738900466432	27.186705062701577	20.99553055964243
68-69	22.917721352267243	28.3001873365122	27.50601599676284	21.276075314457717
70-71	22.85034741079577	28.209622243249143	27.58789969038168	21.352130655573408
72-73	23.111654959477203	28.311604572192927	27.352705552479357	21.224034915850517
74-75	22.777852087062794	28.26409896530937	27.53196418984658	21.42608475778125
76-77	22.69713507845835	27.819735075956352	27.674017121975425	21.80911272360988
78-79	22.613076047346024	28.027489726753203	27.553541855392442	21.805892370508325
80-81	22.70627325871559	28.04883923865314	27.52946726936123	21.71542023327004
82-83	22.65058492215096	27.78573064383611	27.71837225805351	21.845312175959425
84-85	22.6790963572485	27.7730993970075	27.71956813251249	21.828236113231508
86-87	22.601370768240614	27.81237065585031	27.737533959948223	21.848724615960847
88-89	22.620263317195054	27.825101963013676	27.693799448544382	21.860835271246888
90-91	22.61095966788732	27.78360704541068	27.737766211748323	21.867667074953673
92-93	22.66575496536387	27.787173129521232	27.7111014544618	21.835970450653104
94-95	22.52593771839256	27.871980322199125	27.72156305480452	21.8805189046038
96-97	22.63525305410122	27.94451210831844	27.784465077532257	21.635769760048085
98-99	23.691763518945045	29.86302901134111	27.978576486090883	18.466630983622967
100	30.109897315096006	40.676159543011394	29.213943141892596	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	454.0
1	426.0
2	400.5
3	456.0
4	650.5
5	966.0
6	1198.0
7	1396.5
8	1671.0
9	1902.0
10	2032.0
11	2153.0
12	2488.5
13	3165.0
14	4445.5
15	6021.5
16	7387.0
17	8527.5
18	9006.0
19	8650.0
20	8048.5
21	7612.5
22	7805.0
23	9211.5
24	11783.5
25	15405.5
26	20066.0
27	25618.0
28	31501.5
29	38108.0
30	44533.0
31	50601.0
32	57714.0
33	65031.5
34	72075.5
35	79437.5
36	87295.0
37	93473.0
38	97902.5
39	102048.5
40	106478.0
41	109390.5
42	110854.0
43	113116.5
44	115208.5
45	116633.5
46	116637.5
47	115987.5
48	114800.5
49	112442.0
50	109906.0
51	106448.0
52	100953.0
53	94102.0
54	86610.5
55	79525.0
56	72423.0
57	64728.0
58	57341.5
59	49055.5
60	40345.0
61	32670.5
62	25848.5
63	19896.0
64	14906.0
65	11024.0
66	8136.5
67	6196.5
68	4886.5
69	3786.0
70	2792.5
71	1955.5
72	1350.0
73	900.0
74	610.0
75	411.5
76	259.0
77	158.5
78	91.0
79	53.5
80	41.0
81	30.0
82	21.5
83	16.0
84	12.0
85	8.5
86	4.5
87	3.0
88	2.5
89	2.5
90	2.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.009337500540891799
3	8.350610239821934E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.8978843334392984E-5
22-23	0.0
24-25	1.898092189578411E-5
26-27	9.491630185586048E-5
28-29	9.494827692614448E-4
30-31	0.002033954494165022
32-33	0.001295507560810744
34-35	2.866146378299221E-4
36-37	0.0
38-39	0.0
40-41	5.8368961098643935E-5
42-43	0.001274019930767797
44-45	3.954101579683351E-5
46-47	3.792146464671665E-4
48-49	0.0010274477633469494
50-51	9.555646187358165E-4
52-53	0.0
54-55	0.002913977523520035
56-57	0.047901289560271586
58-59	0.0655582949874852
60-61	0.04170241057787383
62-63	0.006544553172241481
64-65	0.0
66-67	0.0
68-69	4.427187268471941E-5
70-71	0.0014556888319552813
72-73	0.006909649200508279
74-75	0.011679444589737752
76-77	0.012647367309196163
78-79	0.0037040458026362487
80-81	0.006217363292165054
82-83	0.04580578060784535
84-85	0.037010872642500976
86-87	0.008164230881498402
88-89	0.001967133433399834
90-91	0.0028003247367568607
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	10.0
20-21	69.0
22-23	194.0
24-25	255.0
26-27	653.0
28-29	1723.0
30-31	5587.0
32-33	7113.0
34-35	10043.0
36-37	16265.0
38-39	18284.0
40-41	18515.0
42-43	21002.0
44-45	23689.0
46-47	23379.0
48-49	23039.0
50-51	21043.0
52-53	19134.0
54-55	21438.0
56-57	22465.0
58-59	22509.0
60-61	22318.0
62-63	22701.0
64-65	23780.0
66-67	24202.0
68-69	25721.0
70-71	26371.0
72-73	23826.0
74-75	24301.0
76-77	25636.0
78-79	25743.0
80-81	25781.0
82-83	24315.0
84-85	24051.0
86-87	24895.0
88-89	26004.0
90-91	26102.0
92-93	25366.0
94-95	28366.0
96-97	80170.0
98-99	577858.0
100-101	1250622.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.64829493808857
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.83951497352747	86.00231545141024
2	4.719770692859177	8.65117872998609
3	0.8767212897496166	2.4105003402442278
4	0.28257680596187806	1.035907298218289
5	0.11752355268790468	0.538541660945654
6	0.05450235405319734	0.2997028691452534
7	0.03496927116839157	0.22434118544675236
8	0.022205647575287736	0.16280877906169774
9	0.014092606912288972	0.11624070552696049
>10	0.0373151078756434	0.5026098910019778
>50	7.557610025904927E-4	0.048856031201265276
>100	5.1936626604886E-5	0.006997057811565735
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	3.7957319271917886E-5	0.0	0.0	0.0
5	0.0	3.7957319271917886E-5	0.0	0.0	0.0
6	3.7957319271917886E-5	7.591463854383577E-5	0.0	0.0	0.0
7	3.7957319271917886E-5	7.591463854383577E-5	0.0	0.0	3.7957319271917886E-5
8	3.7957319271917886E-5	1.1387195781575365E-4	0.0	0.0	7.591463854383577E-5
9	3.7957319271917886E-5	1.5182927708767155E-4	0.0	0.0	7.591463854383577E-5
10-11	1.1387195781575365E-4	1.5182927708767155E-4	0.0	0.0	7.591463854383577E-5
12-13	1.1387195781575365E-4	1.5182927708767155E-4	0.0	0.0	7.591463854383577E-5
14-15	1.1387195781575365E-4	1.5182927708767155E-4	0.0	3.7957319271917886E-5	7.591463854383577E-5
16-17	1.5182927708767155E-4	1.5182927708767155E-4	0.0	3.7957319271917886E-5	7.591463854383577E-5
18-19	1.8978659635958943E-4	1.8978659635958943E-4	0.0	3.7957319271917886E-5	7.591463854383577E-5
20-21	1.8978659635958943E-4	1.8978659635958943E-4	0.0	3.7957319271917886E-5	7.591463854383577E-5
22-23	1.8978659635958943E-4	2.277439156315073E-4	0.0	3.7957319271917886E-5	7.591463854383577E-5
24-25	1.8978659635958943E-4	2.846798945393841E-4	0.0	9.489329817979471E-5	7.591463854383577E-5
26-27	1.8978659635958943E-4	3.036585541753431E-4	0.0	2.0876525599554835E-4	7.591463854383577E-5
28-29	1.8978659635958943E-4	3.036585541753431E-4	0.0	6.073171083506862E-4	9.489329817979471E-5
30-31	1.8978659635958943E-4	3.036585541753431E-4	0.0	0.0016891007076003457	1.1387195781575365E-4
32-33	2.277439156315073E-4	3.036585541753431E-4	0.0	0.004763643568625695	1.1387195781575365E-4
34-35	2.4672257526746626E-4	3.41615873447261E-4	0.0	0.00916669260416817	1.1387195781575365E-4
36-37	2.657012349034252E-4	3.7957319271917885E-4	0.0	0.014860290494955852	1.1387195781575365E-4
38-39	2.657012349034252E-4	3.7957319271917885E-4	0.0	0.02884756264665759	1.1387195781575365E-4
40-41	3.036585541753431E-4	3.7957319271917885E-4	0.0	0.04365091716270557	1.1387195781575365E-4
42-43	3.036585541753431E-4	4.365091716270557E-4	0.0	0.05296943904396141	1.1387195781575365E-4
44-45	3.036585541753431E-4	4.934451505349325E-4	0.0	0.06477416533752788	1.1387195781575365E-4
46-47	3.41615873447261E-4	5.314024698068504E-4	0.0	0.0761803397787392	1.1387195781575365E-4
48-49	3.7957319271917885E-4	5.314024698068504E-4	0.0	0.0872259196868673	1.1387195781575365E-4
50-51	3.7957319271917885E-4	7.591463854383577E-4	0.0	0.09876494474553034	1.1387195781575365E-4
52-53	3.7957319271917885E-4	7.591463854383577E-4	0.0	0.12598034266349545	1.328506174517126E-4
54-55	3.7957319271917885E-4	7.591463854383577E-4	0.0	0.15636517674066575	1.5182927708767155E-4
56-57	3.7957319271917885E-4	7.591463854383577E-4	0.0	0.19396190147950038	1.5182927708767155E-4
58-59	3.7957319271917885E-4	7.591463854383577E-4	0.0	0.22772493697187135	1.5182927708767155E-4
60-61	4.744664908989736E-4	7.591463854383577E-4	0.0	0.24106693469595047	1.5182927708767155E-4
62-63	4.934451505349325E-4	7.591463854383577E-4	0.0	0.2549403348898365	1.5182927708767155E-4
64-65	4.934451505349325E-4	7.971037047102756E-4	0.0	0.2632719664700225	1.5182927708767155E-4
66-67	4.934451505349325E-4	8.730183432541114E-4	0.0	0.270426921152779	1.5182927708767155E-4
68-69	5.314024698068504E-4	8.730183432541114E-4	0.0	0.27760085449517147	1.5182927708767155E-4
70-71	5.314024698068504E-4	8.730183432541114E-4	0.0	0.28346526032268277	1.5182927708767155E-4
72-73	5.693597890787682E-4	8.730183432541114E-4	0.0	0.28838073316839613	1.8978659635958943E-4
74-75	5.693597890787682E-4	8.730183432541114E-4	0.0	0.29206259313777216	1.8978659635958943E-4
76-77	5.693597890787682E-4	8.730183432541114E-4	0.0	0.29610504764023143	1.8978659635958943E-4
78-79	5.693597890787682E-4	8.730183432541114E-4	0.0	0.2984204441158184	1.8978659635958943E-4
80-81	5.693597890787682E-4	8.730183432541114E-4	0.0	0.29904673988380504	2.0876525599554835E-4
82-83	5.693597890787682E-4	8.919970028900703E-4	0.0	0.29959712101324787	2.657012349034252E-4
84-85	5.693597890787682E-4	9.489329817979471E-4	0.0	0.2998248649288794	2.657012349034252E-4
86-87	5.693597890787682E-4	9.86890301069865E-4	0.0	0.2999007795674232	2.657012349034252E-4
88	5.693597890787682E-4	0.0010248476203417829	0.0	0.2999007795674232	2.657012349034252E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3465	0.0	44.8974	1
GTATCAA	6140	0.0	36.39197	1
TCAACGC	6860	0.0	31.583693	4
ATCAACG	6920	0.0	31.310438	3
TATCAAC	7125	0.0	30.710052	2
CAACGCA	7100	0.0	30.516071	5
AACGCAG	7375	0.0	29.43534	6
ACGCAGA	8415	0.0	25.847553	7
CGCAGAG	8620	0.0	25.23285	8
TGGTATC	1755	0.0	23.782427	2
GTGGTAT	2005	0.0	22.078674	1
GCAGAGT	10210	0.0	21.385918	9
GAGTACT	5345	0.0	19.124403	12-13
GTACATG	8385	0.0	18.804708	1
TACATGG	8375	0.0	18.726482	2
CAGAGTA	9885	0.0	18.656288	10-11
GTACTTT	5825	0.0	17.512304	14-15
ACATGGG	8530	0.0	17.44447	3
AGAGTAC	9140	0.0	17.317596	10-11
CATGGGG	5950	0.0	16.365108	4
>>END_MODULE