FastQCFastQC Report
Fri 27 May 2016
SRR2139471_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139471_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4617311
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66650.050.0488621
GTATCAA118400.037.8537861
TCAACGC137550.031.8046994
TATCAAC140200.031.632282
ATCAACG140650.031.1949183
CAACGCA142400.030.7214645
AACGCAG147900.029.607936
ACGCAGA169550.025.8272637
GTGGTAT32200.025.7659171
CGCAGAG175500.024.9760078
TGGTATC31300.023.7741342
GCAGAGT203800.021.5707479
GAGTACT111700.020.40564312-13
GTACATG156350.019.2290211
CAGAGTA201150.019.1549510-11
GATCAAC21500.019.0955661
TACATGG155600.018.7720152
GTACTTT121450.018.64444414-15
ACATGGG153750.018.2736993
AGAGTAC185250.017.45178410-11
Produced by FastQC (version 0.11.5)