##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139471_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4617311
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.922971617029912	28.0	18.0	33.0	18.0	33.0
2	30.14430411986544	32.0	27.0	33.0	27.0	33.0
3	27.483835071971544	29.0	25.0	31.0	18.0	33.0
4	31.17022526747711	32.0	31.0	33.0	29.0	33.0
5	32.595897482322506	33.0	33.0	33.0	32.0	33.0
6	37.028781037274726	38.0	37.0	38.0	35.0	38.0
7	37.03633218555129	38.0	38.0	38.0	35.0	38.0
8	37.3120790434086	38.0	38.0	38.0	36.0	38.0
9	37.47580550671159	38.0	38.0	38.0	37.0	38.0
10-11	37.37003604478884	38.0	38.0	38.0	37.0	38.0
12-13	37.571934184203755	38.0	38.0	38.0	37.5	38.0
14-15	37.546889953914736	38.0	38.0	38.0	37.5	38.0
16-17	37.5997447432066	38.0	38.0	38.0	38.0	38.0
18-19	37.5922010668114	38.0	38.0	38.0	38.0	38.0
20-21	37.59777949306319	38.0	38.0	38.0	38.0	38.0
22-23	37.60826353632998	38.0	38.0	38.0	38.0	38.0
24-25	37.61166112020318	38.0	38.0	38.0	38.0	38.0
26-27	37.57852094364986	38.0	38.0	38.0	38.0	38.0
28-29	37.55762200267547	38.0	38.0	38.0	38.0	38.0
30-31	37.55908105132187	38.0	38.0	38.0	38.0	38.0
32-33	37.47480492626429	38.0	38.0	38.0	38.0	38.0
34-35	37.51850357967056	38.0	38.0	38.0	38.0	38.0
36-37	37.504611084643244	38.0	38.0	38.0	38.0	38.0
38-39	37.49708536043874	38.0	38.0	38.0	38.0	38.0
40-41	37.487069008289545	38.0	38.0	38.0	38.0	38.0
42-43	37.45289821974959	38.0	38.0	38.0	37.5	38.0
44-45	37.421019213894326	38.0	38.0	38.0	37.0	38.0
46-47	37.396810558004475	38.0	38.0	38.0	37.0	38.0
48-49	37.28761717334367	38.0	38.0	38.0	37.0	38.0
50-51	37.285166163783856	38.0	38.0	38.0	37.0	38.0
52-53	37.29587748536753	38.0	38.0	38.0	37.0	38.0
54-55	37.28209588736431	38.0	38.0	38.0	37.0	38.0
56-57	37.236639837419574	38.0	38.0	38.0	37.0	38.0
58-59	37.22187100613884	38.0	38.0	38.0	37.0	38.0
60-61	37.23704157551936	38.0	38.0	38.0	37.0	38.0
62-63	37.24296600831941	38.0	38.0	38.0	37.0	38.0
64-65	37.24978640451903	38.0	38.0	38.0	37.0	38.0
66-67	37.189480020930134	38.0	38.0	38.0	37.0	38.0
68-69	37.20755943740349	38.0	38.0	38.0	37.0	38.0
70-71	37.22774346703325	38.0	38.0	38.0	37.0	38.0
72-73	37.241860205090504	38.0	38.0	38.0	37.0	38.0
74-75	37.22857935765745	38.0	38.0	38.0	37.0	38.0
76-77	37.20167196264596	38.0	38.0	38.0	36.0	38.0
78-79	37.20209555312424	38.0	38.0	38.0	36.0	38.0
80-81	37.20016296003855	38.0	38.0	38.0	36.0	38.0
82-83	37.15178429430722	38.0	38.0	38.0	36.0	38.0
84-85	37.1513836056897	38.0	38.0	38.0	36.0	38.0
86-87	37.145730782235916	38.0	38.0	38.0	36.0	38.0
88-89	37.12574809199415	38.0	38.0	38.0	36.0	38.0
90-91	37.10353509651499	38.0	38.0	38.0	36.0	38.0
92-93	37.091264278094286	38.0	38.0	38.0	36.0	38.0
94-95	37.08716809509794	38.0	38.0	38.0	36.0	38.0
96-97	37.06622729199833	38.0	38.0	38.0	36.0	38.0
98-99	37.08450822618664	38.0	38.0	38.0	36.0	38.0
100	35.77323814352943	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	16.0
21	51.0
22	210.0
23	568.0
24	1365.0
25	2832.0
26	5195.0
27	8856.0
28	14537.0
29	21461.0
30	28516.0
31	36499.0
32	47046.0
33	63468.0
34	99022.0
35	203909.0
36	770416.0
37	3313343.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.93454458666527	17.87834954154052	11.840657906734027	22.346447965060186
2	14.919118328702488	20.192151909043098	41.75096623945064	23.13776352280377
3	19.500380091438522	25.528933513236062	29.51679657636156	25.453889818963855
4	13.12406723307137	17.6011752294788	39.053812922716276	30.220944614733554
5	13.955763430273594	37.48356998261542	34.74788681117646	13.812779775934523
6	30.450926957270152	37.20864806377565	18.759793308269685	13.580631670684518
7	27.36597123304018	32.60917447406077	22.549163355035	17.47569093786405
8	24.64830720737676	36.66772716847533	20.776399943603536	17.90756568054437
9	26.012131303262876	17.59651883964498	21.334798544001043	35.056551313091106
10-11	24.72185650912403	26.83447140554318	28.233023506538764	20.21064857879402
12-13	25.59661889788234	24.246070927429404	28.56009699151736	21.597213183170897
14-15	22.764234421289796	25.574279055493555	26.3957203662478	25.26576615696885
16-17	22.194357278511237	28.743179742495144	27.021571646354335	22.04089133263928
18-19	22.12725112083635	27.790839733342633	28.86317382563141	21.21873532018961
20-21	23.017656434751412	26.915588729570306	28.802082876488555	21.264671959189723
22-23	22.82751112628656	26.725450235986543	28.674831303977932	21.77220733374896
24-25	22.5152850137661	26.890371789670585	28.72002481384741	21.874318382715906
26-27	22.382652672185664	27.075126898275155	28.705644092146603	21.83657633739258
28-29	22.394668089919318	27.05665479503201	28.744882070797935	21.80379504425074
30-31	22.646546111515594	26.95206191476211	28.617855687603715	21.78353628611858
32-33	22.42295481202228	27.119154226855475	28.56998802327019	21.88790293785205
34-35	22.572730482073393	27.132380516989517	28.58811753906864	21.70677146186845
36-37	22.595903006925266	26.984513821010207	28.514052799954513	21.905530372110007
38-39	22.389398658515386	27.197383925780404	28.612976422148407	21.800240993555803
40-41	22.659299951063865	27.048206241188204	28.452450905927	21.840042901820926
42-43	22.567270127205806	27.127065628824838	28.293474193369168	22.01219005060019
44-45	22.57566941858781	27.199587924121772	28.239435648085674	21.985307009204742
46-47	22.668241304187227	27.228079472056727	27.938340700258152	22.16533852349789
48-49	22.62344591671519	27.324530740319553	27.88714722240983	22.164876120555427
50-51	22.595438737669518	27.49033978275421	27.793390540478846	22.12083093909742
52-53	22.800131597543203	27.40535055222314	27.648435309845027	22.14608254038863
54-55	22.77141879470467	27.31459436799349	27.66500755795443	22.24897927934741
56-57	22.753540320268396	27.432662235155703	27.695841068232994	22.117956376342907
58-59	22.71644200686306	27.42504577685582	27.72970652367821	22.12880569260291
60-61	22.815847300286176	27.416182727326248	27.71876347655194	22.04920649583564
62-63	22.82026269783165	27.515330110037684	27.654217307271857	22.01018988485881
64-65	22.79513802784577	27.441707583435687	27.636530238537716	22.12662415018083
66-67	22.999981485652548	27.498085113765764	27.420263548670725	22.081669851910966
68-69	22.73622081343514	27.45182735104749	27.649675104046388	22.162276731470982
70-71	22.701344608140094	27.325110385271138	27.743119266055043	22.230425740533722
72-73	22.89503831157035	27.357873901045597	27.5680426071005	22.179045180283552
74-75	22.57993704492703	27.491297574004008	27.634491749069984	22.29427363199898
76-77	22.737539790046412	27.251427653836767	27.653592884431312	22.357439671685505
78-79	22.64383571231017	27.362164975630463	27.574159041818447	22.41984027024092
80-81	22.66446754013055	27.37983218069417	27.57718812414079	22.378512155034493
82-83	22.736533545238125	27.302821775861762	27.626813977518612	22.3338307013815
84-85	22.764389513334525	27.310707211647596	27.5424628443023	22.38244043071558
86-87	22.604249922350817	27.333873173627886	27.681078840119184	22.380798063902112
88-89	22.732888213222363	27.309566146640435	27.5697606341302	22.387785006007
90-91	22.775697831945724	27.38738565357458	27.508356250158943	22.328560264320753
92-93	22.79774994765818	27.383227026524626	27.522198559380772	22.296824466436423
94-95	22.613063491827205	27.42436196270599	27.53898356116752	22.423590984299285
96-97	22.80467959387175	27.49535140153711	27.557071330216303	22.142897674374836
98-99	23.853570141024935	29.45045008711115	27.76595223925996	18.930027532603955
100	30.5795289781869	40.41508455112237	29.005386470690723	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	532.0
1	536.0
2	739.0
3	1065.5
4	1445.0
5	2109.5
6	2668.5
7	3241.5
8	4135.5
9	4798.0
10	4943.5
11	5041.5
12	5644.5
13	7062.0
14	9559.5
15	12461.5
16	15186.0
17	17467.0
18	18054.5
19	16895.0
20	15209.5
21	13808.5
22	13651.5
23	15261.5
24	18662.0
25	24012.5
26	31546.0
27	40405.5
28	49293.5
29	59119.0
30	69266.0
31	79126.5
32	90338.5
33	102487.0
34	113976.0
35	125104.0
36	137344.5
37	147379.0
38	155771.5
39	163382.5
40	169954.5
41	175948.0
42	179709.0
43	183518.5
44	188267.0
45	192337.0
46	194092.0
47	195275.0
48	195452.5
49	194006.0
50	192070.0
51	188411.0
52	181688.0
53	172677.5
54	162764.0
55	152252.0
56	139797.5
57	126026.0
58	112862.0
59	97944.5
60	81963.0
61	66383.0
62	52606.0
63	41014.0
64	30538.0
65	22818.5
66	17324.0
67	13265.0
68	10188.5
69	7641.5
70	5650.0
71	4016.0
72	2819.0
73	1931.5
74	1309.5
75	851.5
76	535.0
77	342.0
78	213.0
79	141.0
80	93.0
81	53.5
82	33.5
83	24.0
84	18.5
85	12.0
86	5.5
87	2.5
88	2.5
89	3.5
90	4.0
91	3.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.009594328820389183
3	2.1657627134061363E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.3826088613988772E-4
26-27	3.141036548559721E-4
28-29	0.0027086139231639905
30-31	0.011776985536321862
32-33	0.007062533628525508
34-35	5.990875569732268E-4
36-37	7.653429113447545E-5
38-39	6.597097343139992E-4
40-41	0.0
42-43	0.001336048927002406
44-45	7.51548245471361E-4
46-47	0.0016290648703813043
48-49	0.0031486821396253433
50-51	0.0021963050559632327
52-53	0.0
54-55	0.0014238068440294294
56-57	0.04768136004697314
58-59	0.06527402755576939
60-61	0.044409514328796175
62-63	0.012877696011374815
64-65	0.0
66-67	0.0
68-69	1.3610521081274237E-4
70-71	0.0010991566720433747
72-73	0.004903822056559599
74-75	0.008940092857425357
76-77	0.0173512830131581
78-79	0.012323868144718756
80-81	0.006046752390070499
82-83	0.035950945463605284
84-85	0.025588389541739694
86-87	0.007272768411619298
88-89	0.0019723680754407664
90-91	0.003134139952820197
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	26.0
20-21	107.0
22-23	321.0
24-25	380.0
26-27	1042.0
28-29	2765.0
30-31	8503.0
32-33	10552.0
34-35	15018.0
36-37	23965.0
38-39	28743.0
40-41	27704.0
42-43	31698.0
44-45	37253.0
46-47	37475.0
48-49	35252.0
50-51	32572.0
52-53	31488.0
54-55	33639.0
56-57	34807.0
58-59	34863.0
60-61	34213.0
62-63	33181.0
64-65	34769.0
66-67	36607.0
68-69	37631.0
70-71	37716.0
72-73	34648.0
74-75	35339.0
76-77	37035.0
78-79	38198.0
80-81	37888.0
82-83	35487.0
84-85	35008.0
86-87	36338.0
88-89	37798.0
90-91	38960.0
92-93	37752.0
94-95	43796.0
96-97	140726.0
98-99	1084541.0
100-101	2301507.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.76429041629774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.89400361906435	81.56906024758943
2	5.992850699818669	10.63902279880435
3	1.2611925441665337	3.358465837838029
4	0.41347823800548617	1.4680840959655221
5	0.17730092783195794	0.786899552457748
6	0.0953029349261486	0.5075698435986108
7	0.04915749921806104	0.30544013757116295
8	0.03262709938306818	0.2316897060064058
9	0.02186574448303898	0.1746807564114929
>10	0.060528660719869794	0.8105628546245911
>50	0.001139617865042441	0.0680870084848428
>100	5.524145177684914E-4	0.0804371606478032
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.3315254268122726E-5
2	0.0	0.0	0.0	2.1657627134061363E-5	4.3315254268122726E-5
3	0.0	0.0	0.0	2.1657627134061363E-5	4.3315254268122726E-5
4	0.0	2.1657627134061363E-5	0.0	2.1657627134061363E-5	4.3315254268122726E-5
5	0.0	2.1657627134061363E-5	0.0	2.1657627134061363E-5	4.3315254268122726E-5
6	2.1657627134061363E-5	2.1657627134061363E-5	0.0	2.1657627134061363E-5	4.3315254268122726E-5
7	2.1657627134061363E-5	2.1657627134061363E-5	0.0	2.1657627134061363E-5	4.3315254268122726E-5
8	2.1657627134061363E-5	2.1657627134061363E-5	0.0	2.1657627134061363E-5	4.3315254268122726E-5
9	2.1657627134061363E-5	4.3315254268122726E-5	0.0	2.1657627134061363E-5	4.3315254268122726E-5
10-11	2.1657627134061363E-5	4.3315254268122726E-5	0.0	4.3315254268122726E-5	4.3315254268122726E-5
12-13	2.1657627134061363E-5	4.3315254268122726E-5	0.0	6.49728814021841E-5	4.3315254268122726E-5
14-15	2.1657627134061363E-5	5.4144067835153404E-5	0.0	8.663050853624545E-5	1.191169492373375E-4
16-17	2.1657627134061363E-5	6.497288140218408E-5	0.0	8.663050853624545E-5	1.732610170724909E-4
18-19	2.1657627134061363E-5	8.663050853624545E-5	0.0	8.663050853624545E-5	1.732610170724909E-4
20-21	3.248644070109205E-5	1.0828813567030681E-4	0.0	9.745932210327613E-5	1.732610170724909E-4
22-23	4.3315254268122726E-5	1.0828813567030681E-4	0.0	1.0828813567030681E-4	1.9491864420655226E-4
24-25	9.745932210327612E-5	1.2994576280436816E-4	0.0	1.4077457637139883E-4	2.1657627134061361E-4
26-27	1.5160338993842952E-4	1.2994576280436816E-4	0.0	1.732610170724909E-4	2.1657627134061361E-4
28-29	1.5160338993842952E-4	1.2994576280436816E-4	0.0	3.356932205779511E-4	2.1657627134061361E-4
30-31	1.5160338993842952E-4	1.5160338993842952E-4	0.0	0.0011370254245382215	2.3823389847467497E-4
32-33	1.840898306395216E-4	1.5160338993842952E-4	0.0	0.002988752544500468	2.598915256087363E-4
34-35	1.9491864420655226E-4	1.9491864420655223E-4	0.0	0.005165344071473635	2.815491527427977E-4
36-37	1.9491864420655226E-4	2.598915256087363E-4	0.0	0.008565591531521269	2.815491527427977E-4
38-39	1.9491864420655226E-4	2.815491527427977E-4	0.0	0.013785079670830057	2.923779663098284E-4
40-41	2.1657627134061361E-4	2.815491527427977E-4	0.0	0.018972081369437753	3.0320677987685904E-4
42-43	2.1657627134061361E-4	2.815491527427977E-4	0.0	0.024570577983592613	3.0320677987685904E-4
44-45	2.274050849076443E-4	2.815491527427977E-4	0.0	0.030190732224881535	3.0320677987685904E-4
46-47	2.70720339175767E-4	3.0320677987685904E-4	0.0	0.036330669517387934	3.248644070109204E-4
48-49	2.815491527427977E-4	3.0320677987685904E-4	0.0	0.04278464240333822	3.465220341449818E-4
50-51	3.0320677987685904E-4	3.248644070109204E-4	0.0	0.048708003424504	3.465220341449818E-4
52-53	3.0320677987685904E-4	3.248644070109204E-4	0.0	0.05480462546274228	3.465220341449818E-4
54-55	3.0320677987685904E-4	3.248644070109204E-4	0.0	0.060857932246712425	3.465220341449818E-4
56-57	3.0320677987685904E-4	3.248644070109204E-4	0.0	0.06704118479348695	3.465220341449818E-4
58-59	3.0320677987685904E-4	3.465220341449818E-4	0.0	0.07335438310306583	3.465220341449818E-4
60-61	3.248644070109204E-4	3.790084748460738E-4	0.0	0.07987332887041831	3.465220341449818E-4
62-63	3.6817966127904313E-4	3.898372884131045E-4	0.0	0.08622984243426532	3.465220341449818E-4
64-65	3.6817966127904313E-4	4.548101698152886E-4	0.0	0.09194745599765751	3.465220341449818E-4
66-67	3.790084748460738E-4	4.548101698152886E-4	0.0	0.09718860176410037	3.465220341449818E-4
68-69	3.898372884131045E-4	4.548101698152886E-4	0.0	0.1027437831239871	3.465220341449818E-4
70-71	4.1149491554716585E-4	4.8729661051638063E-4	0.0	0.10748680346634654	3.898372884131045E-4
72-73	4.1149491554716585E-4	5.197830512174727E-4	0.0	0.11037809668874374	4.1149491554716585E-4
74-75	4.1149491554716585E-4	5.197830512174727E-4	0.0	0.11289038143629485	4.1149491554716585E-4
76-77	4.1149491554716585E-4	5.197830512174727E-4	0.0	0.1160199085571667	4.2232372911419654E-4
78-79	4.1149491554716585E-4	5.197830512174727E-4	0.0	0.11772003228719052	4.3315254268122723E-4
80-81	4.1149491554716585E-4	5.197830512174727E-4	0.0	0.11846722042331564	4.3315254268122723E-4
82-83	4.2232372911419654E-4	5.197830512174727E-4	0.0	0.11898700347453312	4.3315254268122723E-4
84-85	4.3315254268122723E-4	5.197830512174727E-4	0.0	0.11921440855944077	4.3315254268122723E-4
86-87	4.3315254268122723E-4	5.41440678351534E-4	0.0	0.11925772381370889	4.3315254268122723E-4
88	4.3315254268122723E-4	5.847559326196568E-4	0.0	0.11926855262727593	4.548101698152886E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6665	0.0	50.048862	1
GTATCAA	11840	0.0	37.853786	1
TCAACGC	13755	0.0	31.804699	4
TATCAAC	14020	0.0	31.63228	2
ATCAACG	14065	0.0	31.194918	3
CAACGCA	14240	0.0	30.721464	5
AACGCAG	14790	0.0	29.60793	6
ACGCAGA	16955	0.0	25.827263	7
GTGGTAT	3220	0.0	25.765917	1
CGCAGAG	17550	0.0	24.976007	8
TGGTATC	3130	0.0	23.774134	2
GCAGAGT	20380	0.0	21.570747	9
GAGTACT	11170	0.0	20.405643	12-13
GTACATG	15635	0.0	19.229021	1
CAGAGTA	20115	0.0	19.15495	10-11
GATCAAC	2150	0.0	19.095566	1
TACATGG	15560	0.0	18.772015	2
GTACTTT	12145	0.0	18.644444	14-15
ACATGGG	15375	0.0	18.273699	3
AGAGTAC	18525	0.0	17.451784	10-11
>>END_MODULE