FastQCFastQC Report
Fri 27 May 2016
SRR2139470_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139470_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4215916
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58850.052.2605861
GTATCAA114000.039.962431
TCAACGC127250.035.093614
ATCAACG129700.034.43073
TATCAAC131300.034.2746622
CAACGCA132200.033.8441165
AACGCAG135350.033.056466
ACGCAGA157150.028.4979747
CGCAGAG163400.027.3818348
GCAGAGT186600.024.0231639
TGGTATC26000.023.1329632
GTGGTAT28600.023.1180481
GAGTACT103700.021.5110712-13
CAGAGTA184000.020.51467110-11
GTACATG149050.020.0618741
GTACTTT112100.019.91832414-15
TACATGG149600.019.4463582
ACATGGG150250.018.9911273
AGAGTAC170400.018.57263210-11
CATGGGG105050.016.9308644
Produced by FastQC (version 0.11.5)