##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139470_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4215916
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.874627957483025	28.0	18.0	33.0	18.0	33.0
2	30.04345674818948	32.0	27.0	33.0	27.0	33.0
3	27.353200585590415	29.0	25.0	31.0	18.0	33.0
4	31.14802761724854	32.0	31.0	33.0	29.0	33.0
5	32.601592868548614	33.0	33.0	33.0	32.0	33.0
6	37.03700429515199	38.0	37.0	38.0	35.0	38.0
7	37.06160132222748	38.0	38.0	38.0	35.0	38.0
8	37.320015863693676	38.0	38.0	38.0	36.0	38.0
9	37.48829198684224	38.0	38.0	38.0	37.0	38.0
10-11	37.385375325314826	38.0	38.0	38.0	37.0	38.0
12-13	37.58340322719903	38.0	38.0	38.0	37.5	38.0
14-15	37.5553305616146	38.0	38.0	38.0	37.5	38.0
16-17	37.61123976379036	38.0	38.0	38.0	38.0	38.0
18-19	37.60327660702917	38.0	38.0	38.0	38.0	38.0
20-21	37.61119684252661	38.0	38.0	38.0	38.0	38.0
22-23	37.61891570146962	38.0	38.0	38.0	38.0	38.0
24-25	37.622029499867324	38.0	38.0	38.0	38.0	38.0
26-27	37.59202956068712	38.0	38.0	38.0	38.0	38.0
28-29	37.571265632015965	38.0	38.0	38.0	38.0	38.0
30-31	37.572023990881526	38.0	38.0	38.0	38.0	38.0
32-33	37.487340904467004	38.0	38.0	38.0	38.0	38.0
34-35	37.52947239384653	38.0	38.0	38.0	38.0	38.0
36-37	37.51796568028195	38.0	38.0	38.0	38.0	38.0
38-39	37.51332482268364	38.0	38.0	38.0	38.0	38.0
40-41	37.501690783558644	38.0	38.0	38.0	38.0	38.0
42-43	37.46682316318835	38.0	38.0	38.0	38.0	38.0
44-45	37.43054805338862	38.0	38.0	38.0	37.5	38.0
46-47	37.404670525971056	38.0	38.0	38.0	38.0	38.0
48-49	37.28951297585717	38.0	38.0	38.0	37.0	38.0
50-51	37.28530303724351	38.0	38.0	38.0	37.0	38.0
52-53	37.29626175364372	38.0	38.0	38.0	37.0	38.0
54-55	37.28021894112922	38.0	38.0	38.0	37.0	38.0
56-57	37.23399493431067	38.0	38.0	38.0	37.0	38.0
58-59	37.220048338459605	38.0	38.0	38.0	37.0	38.0
60-61	37.23363074338151	38.0	38.0	38.0	37.0	38.0
62-63	37.23919842479789	38.0	38.0	38.0	37.0	38.0
64-65	37.24581878590271	38.0	38.0	38.0	37.0	38.0
66-67	37.187158932917264	38.0	38.0	38.0	36.5	38.0
68-69	37.20563114046013	38.0	38.0	38.0	36.5	38.0
70-71	37.22295891312187	38.0	38.0	38.0	37.0	38.0
72-73	37.23575605964259	38.0	38.0	38.0	37.0	38.0
74-75	37.22473097333851	38.0	38.0	38.0	36.5	38.0
76-77	37.198607311072095	38.0	38.0	38.0	36.0	38.0
78-79	37.19873070452218	38.0	38.0	38.0	36.0	38.0
80-81	37.19726928692724	38.0	38.0	38.0	36.0	38.0
82-83	37.14680967492946	38.0	38.0	38.0	36.0	38.0
84-85	37.144531526643874	38.0	38.0	38.0	36.0	38.0
86-87	37.14233214794419	38.0	38.0	38.0	36.0	38.0
88-89	37.125985614252656	38.0	38.0	38.0	36.0	38.0
90-91	37.101790482656156	38.0	38.0	38.0	36.0	38.0
92-93	37.092157754556155	38.0	38.0	38.0	36.0	38.0
94-95	37.08363473031101	38.0	38.0	38.0	36.0	38.0
96-97	37.06388076218843	38.0	38.0	38.0	36.0	38.0
98-99	37.085200177087415	38.0	38.0	38.0	36.0	38.0
100	35.74920453364333	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	12.0
21	46.0
22	162.0
23	483.0
24	1172.0
25	2431.0
26	4594.0
27	7892.0
28	13008.0
29	19557.0
30	25834.0
31	33066.0
32	42263.0
33	57030.0
34	89158.0
35	184646.0
36	716017.0
37	3018542.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.31858604393447	17.716980129585124	11.828580076073623	22.135853750406792
2	14.9371105735176	20.19851054229943	41.68424863135851	23.180130252824462
3	19.444756094350144	25.571590305587428	29.68400913396458	25.299644466097853
4	13.196135786386638	17.673905267562258	39.01529347358913	30.114665472461976
5	13.958769577002958	37.5120139964838	34.82289495331501	13.706321473198232
6	30.471313944585233	37.260016565794956	18.721957458355433	13.546712031264379
7	27.46124448399826	32.716852043541664	22.507469313904735	17.314434158555343
8	24.7524381415569	36.628362614435396	20.762178373572908	17.857020870434802
9	26.151849325271186	17.60400349532581	21.354908399503216	34.88923877989979
10-11	24.69489430055058	26.8793543324867	28.269787633339945	20.155963733622777
12-13	25.621751002629082	24.333157966145436	28.59599195050376	21.449099080721723
14-15	22.79088340469782	25.64369166748104	26.42308338211672	25.142341545704422
16-17	22.137087171566037	28.85618451601028	27.104987385896685	21.901740926527
18-19	22.15971570591065	27.905940251181477	28.905734839119184	21.02860920378869
20-21	22.983168506003437	26.96924352504159	28.920866159148638	21.126721809806337
22-23	22.816561801350897	26.77380521737068	28.76638063441045	21.64325234686798
24-25	22.507470164414784	26.950332065295264	28.84305443138029	21.699143338909664
26-27	22.389327738080283	27.090085258569896	28.85574299321127	21.664844010138548
28-29	22.350820176352514	27.083773376058417	28.892615968365337	21.672790479223732
30-31	22.593359487657786	26.987053181779526	28.762851673431726	21.656735657130966
32-33	22.33173286036733	27.211836621568054	28.692568647842513	21.763861870222097
34-35	22.523011423725258	27.166131402604986	28.707670426856435	21.603186746813318
36-37	22.526718411978184	27.03367397100658	28.69278122367303	21.746826393342207
38-39	22.340848855882673	27.266658332411986	28.74037616393527	21.65211664777007
40-41	22.61407351528867	27.091362423162803	28.62072036863679	21.67384369291174
42-43	22.503885392388046	27.18647834668139	28.425377051355472	21.88425920957509
44-45	22.500437704377227	27.264811455383946	28.38946290447724	21.845287935761583
46-47	22.64180076039144	27.271648051355978	28.08538659799244	22.001164590260146
48-49	22.593643323675117	27.417661328129732	27.985243586188002	22.003451762007145
50-51	22.56279493598412	27.53268834448775	27.90083699701177	22.003679722516363
52-53	22.779879451387018	27.445412906927736	27.821441810158788	21.95326583152646
54-55	22.741930574380884	27.364183309336106	27.828350017994723	22.065536098288288
56-57	22.664911378819024	27.52543759106738	27.836564727099393	21.973086303014195
58-59	22.680658938186514	27.498256648191933	27.850739676943476	21.97034473667808
60-61	22.77372764615342	27.49319266927269	27.869034212536192	21.86404547203769
62-63	22.733528492032697	27.54396030380004	27.8430202461941	21.879490957973164
64-65	22.776757481618667	27.458970047921618	27.83714490848806	21.927127561971655
66-67	22.997654311054447	27.523526181058557	27.62641412052541	21.852405387361586
68-69	22.708106998807928	27.47942328004888	27.849580819064894	21.962888902078294
70-71	22.67320542094145	27.337773114205156	27.957686627211597	22.031334837641797
72-73	22.84897258524267	27.474737796598365	27.698459961306227	21.977829656852734
74-75	22.557441270410838	27.570482353805865	27.793674494670245	22.078401881113052
76-77	22.661449548933756	27.321627972102952	27.781109603912412	22.235812875050875
78-79	22.578779421609298	27.410334109160505	27.736871188845186	22.274015280385004
80-81	22.5697884571808	27.46387020482836	27.70302234054101	22.26331899744983
82-83	22.714601722272626	27.31029190874062	27.79540490632953	22.179701462657224
84-85	22.683470968022483	27.376761960055873	27.72351264333233	22.21625442858931
86-87	22.58602456199905	27.36224082248395	27.782719955941754	22.26901465957525
88-89	22.665468581064722	27.32434329320706	27.726275723518107	22.28391240221011
90-91	22.68329248434754	27.411920748267914	27.672735723813858	22.232051043570685
92-93	22.710343000991983	27.415785549889215	27.656777068361095	22.21709438075771
94-95	22.56353577801977	27.45314113347822	27.677349499398176	22.30597358910384
96-97	22.773901382876463	27.51301061283143	27.67555472013919	22.037533284152918
98-99	23.81485005238133	29.408645954041436	27.923746107522224	18.85275788605501
100	30.413661664676507	40.47588598163877	29.110452353684725	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	544.0
1	555.0
2	759.5
3	1055.0
4	1369.0
5	1926.0
6	2451.0
7	3046.0
8	4103.5
9	4855.5
10	4931.0
11	5003.0
12	5464.5
13	6633.5
14	8894.0
15	11524.5
16	13930.0
17	16073.5
18	16708.5
19	15524.5
20	13796.0
21	12490.0
22	12314.0
23	13807.5
24	17020.0
25	22089.0
26	29233.5
27	37349.0
28	45657.0
29	55233.5
30	64580.0
31	73404.5
32	83865.5
33	95712.0
34	106695.0
35	116755.0
36	128338.5
37	137761.0
38	144790.0
39	151774.5
40	158237.5
41	163776.5
42	166741.5
43	169490.0
44	173242.5
45	176797.0
46	177910.0
47	177716.0
48	177325.0
49	176342.5
50	174688.0
51	170382.5
52	163571.5
53	154777.0
54	145227.0
55	135819.5
56	124459.5
57	112286.5
58	100544.0
59	86796.5
60	72955.5
61	59682.5
62	47254.5
63	36630.0
64	27816.0
65	21111.0
66	15798.5
67	12001.5
68	9189.5
69	6969.5
70	5196.0
71	3804.5
72	2679.0
73	1756.0
74	1190.5
75	808.5
76	513.5
77	312.0
78	198.0
79	123.5
80	77.0
81	53.5
82	36.5
83	22.5
84	15.0
85	12.5
86	9.5
87	7.0
88	5.0
89	4.0
90	3.0
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.01000968710002761
3	7.115891303337163E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.728061492878277E-4
26-27	1.8979791148750674E-4
28-29	0.0022782816630127142
30-31	0.011853089154946003
32-33	0.0066875281051698285
34-35	5.36831918642049E-4
36-37	2.3950612878220596E-5
38-39	7.707921792535022E-4
40-41	1.2118236176182672E-5
42-43	0.0012073107421509863
44-45	6.266044452547982E-4
46-47	0.0014870052470219313
48-49	0.0032744298399041264
50-51	0.0018521750141590589
52-53	0.0
54-55	0.0012533482302722989
56-57	0.04706358677397165
58-59	0.06618986556435183
60-61	0.044721469833401375
62-63	0.01285374579575398
64-65	0.0
66-67	0.0
68-69	2.0372612363785318E-4
70-71	0.001398869356985014
72-73	0.005413456551542337
74-75	0.009011829808031259
76-77	0.018590813514534055
78-79	0.012751502005832636
80-81	0.006377175527365784
82-83	0.03708662592276615
84-85	0.025922819321767987
86-87	0.008114866152922947
88-89	0.0024588837897036643
90-91	0.003365436872363078
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	19.0
20-21	112.0
22-23	280.0
24-25	336.0
26-27	960.0
28-29	2557.0
30-31	7556.0
32-33	9808.0
34-35	13925.0
36-37	22223.0
38-39	26042.0
40-41	25287.0
42-43	28925.0
44-45	34102.0
46-47	34631.0
48-49	33173.0
50-51	30364.0
52-53	28490.0
54-55	31289.0
56-57	32617.0
58-59	32702.0
60-61	31821.0
62-63	31129.0
64-65	32975.0
66-67	34361.0
68-69	35359.0
70-71	35304.0
72-73	32716.0
74-75	33501.0
76-77	34986.0
78-79	35994.0
80-81	35854.0
82-83	33808.0
84-85	33749.0
86-87	35090.0
88-89	36475.0
90-91	36347.0
92-93	35684.0
94-95	41002.0
96-97	128294.0
98-99	978300.0
100-101	2087769.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.72720060806425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.84052200772582	83.3032014274462
2	5.310376227131199	9.52970386072193
3	1.0767042329807561	2.8982897012464877
4	0.3644591634242565	1.3080760188006213
5	0.1648476560395147	0.7395659351613355
6	0.07661892720832703	0.41248811111977396
7	0.04757768731647504	0.2988308886019177
8	0.029599972046976418	0.21247381038817176
9	0.018057739294134047	0.1458243356555601
>10	0.06887537056517386	0.9392048131482139
>50	0.0016199129836187735	0.0957941091342515
>100	7.044839012189181E-4	0.0989024859429222
>500	3.6619382505183604E-5	0.017644502632599077
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.3719637677790544E-5	0.0	0.0	0.0
3	0.0	2.3719637677790544E-5	0.0	0.0	0.0
4	0.0	4.743927535558109E-5	0.0	2.3719637677790544E-5	0.0
5	0.0	7.115891303337163E-5	0.0	4.743927535558109E-5	0.0
6	4.743927535558109E-5	7.115891303337163E-5	0.0	4.743927535558109E-5	0.0
7	4.743927535558109E-5	7.115891303337163E-5	0.0	4.743927535558109E-5	0.0
8	4.743927535558109E-5	7.115891303337163E-5	0.0	4.743927535558109E-5	0.0
9	4.743927535558109E-5	1.660374637445338E-4	0.0	1.1859818838895272E-4	0.0
10-11	8.30187318722669E-5	1.660374637445338E-4	0.0	1.1859818838895272E-4	0.0
12-13	1.1859818838895272E-4	1.660374637445338E-4	0.0	1.1859818838895272E-4	0.0
14-15	1.4231782606674326E-4	3.2021510865017235E-4	0.0	1.30458007227848E-4	0.0
16-17	2.134767391001149E-4	3.320749274890676E-4	0.0	1.4231782606674326E-4	0.0
18-19	2.134767391001149E-4	3.439347463279629E-4	0.0	1.660374637445338E-4	0.0
20-21	2.134767391001149E-4	3.795142028446487E-4	0.0	1.8975710142232435E-4	0.0
22-23	2.134767391001149E-4	4.269534782002298E-4	0.0	2.2533655793901018E-4	1.1859818838895272E-5
24-25	2.2533655793901018E-4	4.5067311587802035E-4	0.0	2.4905619561680075E-4	2.3719637677790544E-5
26-27	2.3719637677790544E-4	4.7439275355581087E-4	0.0	2.964954709723818E-4	2.3719637677790544E-5
28-29	2.3719637677790544E-4	4.7439275355581087E-4	0.0	4.625329347169156E-4	2.3719637677790544E-5
30-31	2.3719637677790544E-4	4.981123912336014E-4	0.0	0.0013282997099562704	2.3719637677790544E-5
32-33	2.3719637677790544E-4	5.21832028911392E-4	0.0	0.003095412716951666	2.3719637677790544E-5
34-35	2.3719637677790544E-4	6.285703984614494E-4	0.0	0.005111581919563863	4.743927535558109E-5
36-37	2.3719637677790544E-4	7.353087680115069E-4	0.0	0.0087762659407825	4.743927535558109E-5
38-39	2.3719637677790544E-4	7.827480433670879E-4	0.0	0.01369809075892404	4.743927535558109E-5
40-41	2.3719637677790544E-4	8.30187318722669E-4	0.0	0.019355224345077082	4.743927535558109E-5
42-43	2.3719637677790544E-4	8.30187318722669E-4	0.0	0.02390939477921287	5.929909419447636E-5
44-45	2.3719637677790544E-4	8.420471375615643E-4	0.0	0.029151434706004578	7.115891303337163E-5
46-47	2.3719637677790544E-4	8.539069564004596E-4	0.0	0.03499832539357994	7.115891303337163E-5
48-49	2.3719637677790544E-4	8.539069564004596E-4	0.0	0.041212870465161075	7.115891303337163E-5
50-51	2.60916014455696E-4	8.776265940782501E-4	0.0	0.04687000405131411	7.115891303337163E-5
52-53	2.60916014455696E-4	8.776265940782501E-4	0.0	0.05265759564469501	7.115891303337163E-5
54-55	2.60916014455696E-4	8.894864129171455E-4	0.0	0.05826728995549247	7.115891303337163E-5
56-57	2.60916014455696E-4	9.013462317560407E-4	0.0	0.06433951720100686	8.30187318722669E-5
58-59	2.60916014455696E-4	9.013462317560407E-4	0.0	0.07038802480884343	9.487855071116217E-5
60-61	2.846356521334865E-4	9.60645325950517E-4	0.0	0.07612817712686876	9.487855071116217E-5
62-63	3.320749274890676E-4	9.725051447894124E-4	0.0	0.08184460980721628	9.487855071116217E-5
64-65	3.320749274890676E-4	0.0011148229708561556	0.0	0.08725268719775252	9.487855071116217E-5
66-67	3.557945651668582E-4	0.0011148229708561556	0.0	0.09306399842881119	9.487855071116217E-5
68-69	3.557945651668582E-4	0.0011148229708561556	0.0	0.09819929998605284	9.487855071116217E-5
70-71	3.6765438400575344E-4	0.0011504024273728413	0.0	0.1028364891520609	9.487855071116217E-5
72-73	3.795142028446487E-4	0.0011622622462117366	0.0	0.10683324810076861	9.487855071116217E-5
74-75	3.795142028446487E-4	0.0011622622462117366	0.0	0.1101065581003037	9.487855071116217E-5
76-77	3.795142028446487E-4	0.0011859818838895271	0.0	0.11332056900564433	9.487855071116217E-5
78-79	3.795142028446487E-4	0.0011859818838895271	0.0	0.1156569533169067	9.487855071116217E-5
80-81	3.795142028446487E-4	0.0011859818838895271	0.0	0.11643970136027379	9.487855071116217E-5
82-83	3.795142028446487E-4	0.0011859818838895271	0.0	0.11667689773705169	9.487855071116217E-5
84-85	3.795142028446487E-4	0.0011859818838895271	0.0	0.11687851465731292	9.487855071116217E-5
86-87	3.9137402168354395E-4	0.0012334211592451084	0.0	0.11696153338918518	9.487855071116217E-5
88	4.0323384052243927E-4	0.0012808604346006894	0.0	0.11696153338918518	9.487855071116217E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5885	0.0	52.260586	1
GTATCAA	11400	0.0	39.96243	1
TCAACGC	12725	0.0	35.09361	4
ATCAACG	12970	0.0	34.4307	3
TATCAAC	13130	0.0	34.274662	2
CAACGCA	13220	0.0	33.844116	5
AACGCAG	13535	0.0	33.05646	6
ACGCAGA	15715	0.0	28.497974	7
CGCAGAG	16340	0.0	27.381834	8
GCAGAGT	18660	0.0	24.023163	9
TGGTATC	2600	0.0	23.132963	2
GTGGTAT	2860	0.0	23.118048	1
GAGTACT	10370	0.0	21.51107	12-13
CAGAGTA	18400	0.0	20.514671	10-11
GTACATG	14905	0.0	20.061874	1
GTACTTT	11210	0.0	19.918324	14-15
TACATGG	14960	0.0	19.446358	2
ACATGGG	15025	0.0	18.991127	3
AGAGTAC	17040	0.0	18.572632	10-11
CATGGGG	10505	0.0	16.930864	4
>>END_MODULE