FastQCFastQC Report
Fri 27 May 2016
SRR2139469_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139469_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4142344
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60900.049.690681
GTATCAA110900.036.8705831
TCAACGC125650.031.7232744
ATCAACG126800.031.469223
TATCAAC127850.031.3480762
CAACGCA129150.030.8635655
AACGCAG133300.029.8706786
ACGCAGA152150.026.1699737
CGCAGAG157900.025.2980678
GTGGTAT31000.024.3701171
TGGTATC28750.023.605072
GCAGAGT183600.021.6871559
GAGTACT101950.020.61638812-13
GTACATG137200.019.3811681
CAGAGTA180300.019.1135110-11
GTACTTT112350.018.65122214-15
TACATGG138000.018.3718722
ACATGGG135650.018.2474333
AGAGTAC168300.017.45876110-11
ACTTTTT132600.015.90012816-17
Produced by FastQC (version 0.11.5)