##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139469_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4142344
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.831780025994945	28.0	18.0	33.0	18.0	33.0
2	30.01459004853291	32.0	27.0	33.0	25.0	33.0
3	27.34708899116056	29.0	25.0	31.0	18.0	33.0
4	31.165076343249137	32.0	31.0	33.0	29.0	33.0
5	32.58487271940717	33.0	33.0	33.0	32.0	33.0
6	37.04150041618948	38.0	37.0	38.0	35.0	38.0
7	37.09237837321091	38.0	38.0	38.0	35.0	38.0
8	37.331052901449034	38.0	38.0	38.0	36.0	38.0
9	37.48482477553772	38.0	38.0	38.0	37.0	38.0
10-11	37.395397026417896	38.0	38.0	38.0	37.0	38.0
12-13	37.575543943236	38.0	38.0	38.0	37.5	38.0
14-15	37.54594729457524	38.0	38.0	38.0	37.0	38.0
16-17	37.59437229259569	38.0	38.0	38.0	38.0	38.0
18-19	37.589097742727304	38.0	38.0	38.0	38.0	38.0
20-21	37.59532204653744	38.0	38.0	38.0	38.0	38.0
22-23	37.59965796150189	38.0	38.0	38.0	38.0	38.0
24-25	37.60403907098309	38.0	38.0	38.0	38.0	38.0
26-27	37.57427508375099	38.0	38.0	38.0	38.0	38.0
28-29	37.55438349782228	38.0	38.0	38.0	38.0	38.0
30-31	37.54995270815044	38.0	38.0	38.0	38.0	38.0
32-33	37.46226640439225	38.0	38.0	38.0	38.0	38.0
34-35	37.50837597176573	38.0	38.0	38.0	38.0	38.0
36-37	37.49524853885007	38.0	38.0	38.0	38.0	38.0
38-39	37.48598407257019	38.0	38.0	38.0	38.0	38.0
40-41	37.47363463820031	38.0	38.0	38.0	38.0	38.0
42-43	37.435825967870585	38.0	38.0	38.0	37.0	38.0
44-45	37.39889959733218	38.0	38.0	38.0	37.0	38.0
46-47	37.36954020284307	38.0	38.0	38.0	37.0	38.0
48-49	37.25422722211022	38.0	38.0	38.0	37.0	38.0
50-51	37.24784846777506	38.0	38.0	38.0	37.0	38.0
52-53	37.255932122999155	38.0	38.0	38.0	37.0	38.0
54-55	37.23782444860632	38.0	38.0	38.0	37.0	38.0
56-57	37.18721966738741	38.0	38.0	38.0	37.0	38.0
58-59	37.168899814086544	38.0	38.0	38.0	37.0	38.0
60-61	37.18260150053109	38.0	38.0	38.0	37.0	38.0
62-63	37.18603088666863	38.0	38.0	38.0	37.0	38.0
64-65	37.191957425572184	38.0	38.0	38.0	37.0	38.0
66-67	37.122391523754835	38.0	38.0	38.0	36.0	38.0
68-69	37.141407935372925	38.0	38.0	38.0	36.0	38.0
70-71	37.157730320625475	38.0	38.0	38.0	36.0	38.0
72-73	37.17437456287384	38.0	38.0	38.0	36.0	38.0
74-75	37.16016612653343	38.0	38.0	38.0	36.0	38.0
76-77	37.128990603074534	38.0	38.0	38.0	36.0	38.0
78-79	37.12590924350804	38.0	38.0	38.0	36.0	38.0
80-81	37.12341318825345	38.0	38.0	38.0	36.0	38.0
82-83	37.07136762562203	38.0	38.0	38.0	36.0	38.0
84-85	37.06810814154598	38.0	38.0	38.0	36.0	38.0
86-87	37.059233900841065	38.0	38.0	38.0	36.0	38.0
88-89	37.039002457040006	38.0	38.0	38.0	36.0	38.0
90-91	37.01525674093192	38.0	38.0	38.0	36.0	38.0
92-93	37.00074515298569	38.0	38.0	38.0	36.0	38.0
94-95	36.994816873165554	38.0	38.0	38.0	36.0	38.0
96-97	36.97375971363642	38.0	38.0	38.0	35.0	38.0
98-99	36.99468034055397	38.0	38.0	38.0	35.0	38.0
100	35.62924668656171	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	0.0
20	10.0
21	47.0
22	198.0
23	518.0
24	1280.0
25	2763.0
26	4945.0
27	8697.0
28	13987.0
29	20712.0
30	27554.0
31	34967.0
32	44289.0
33	59907.0
34	92220.0
35	186680.0
36	717132.0
37	2926436.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.8920389035773	17.73085480104984	12.338328250864727	22.038778044508135
2	14.812328070610015	20.253927969401943	41.86144151008628	23.07230244990176
3	19.473882293185284	25.63300528227624	29.724940691777572	25.168171732760904
4	13.052971940524497	17.84581386770389	39.30808257353807	29.79313161823354
5	13.91699965043946	37.58297234609197	34.85767961328175	13.642348390186813
6	30.16695378268922	37.55390667699254	18.845996373068	13.433143167250233
7	27.15909156747967	32.844012954983945	22.670401106233573	17.326494371302818
8	24.500065663305605	36.79240545932448	20.889380505337073	17.818148372032837
9	25.89818711338315	17.760234302124594	21.464779361636793	34.87679922285547
10-11	24.568010285963695	27.040257882976405	28.336685702587715	20.05504612847219
12-13	25.49211267823242	24.477481831542722	28.698159785860373	21.332245704364485
14-15	22.640068038772252	25.799293829773674	26.57491748633141	24.985720645122665
16-17	22.00758797434496	28.945580087023192	27.195218456023934	21.851613482607917
18-19	21.946704571131708	28.01803761348647	29.115145917383973	20.92011189799785
20-21	22.829980905519438	27.06588000158849	29.11462277024351	20.989516322648562
22-23	22.61387342378458	26.96529145333997	28.93317215986689	21.487662963008564
24-25	22.303676133903277	27.099404958290584	29.010151478441614	21.586767429364524
26-27	22.253414642362173	27.229292499648967	28.983588443248582	21.533704414740278
28-29	22.189598389480413	27.24607237772424	29.010597659490283	21.55373157330507
30-31	22.426008302816804	27.16320345189802	28.911326516202294	21.49946172908288
32-33	22.209305396164584	27.342041834042323	28.856690096230558	21.59196267356253
34-35	22.371592342749597	27.333938661410507	28.845587057523858	21.448881938316042
36-37	22.37600517810714	27.19244932512735	28.81382583860835	21.617719658157164
38-39	22.21767709970461	27.376026130872315	28.91457121468079	21.491725554742285
40-41	22.45926509650006	27.278075515437592	28.728154688270237	21.534504699792112
42-43	22.381820861450233	27.33001879930067	28.55558023611306	21.732580103136034
44-45	22.418330801803684	27.463149573180473	28.43403842095103	21.68448120406481
46-47	22.528635893382077	27.487378450624206	28.13479206465908	21.849193591334636
48-49	22.475359706277004	27.58411818174874	28.086433898322348	21.854088213651906
50-51	22.4442480338691	27.70274329040824	28.001359528559856	21.851649147162806
52-53	22.62716562277467	27.65077813778909	27.86506942160879	21.856986817827455
54-55	22.669035784932174	27.533405284010858	27.88173861842975	21.91582031262721
56-57	22.55875022809341	27.708255358996364	27.87157615206385	21.861418260846374
58-59	22.581246088921706	27.701332009092848	27.927738973756906	21.78968292822854
60-61	22.684030474072443	27.694824855303462	27.89768788775714	21.723456782866958
62-63	22.64523647418509	27.799546356524342	27.83839584082942	21.716821328461144
64-65	22.660571385124218	27.723841385097064	27.80682555673527	21.80876167304345
66-67	22.85884591426344	27.764438946380544	27.61761288212442	21.759102257231593
68-69	22.604081792898928	27.71464498100717	27.885039941762134	21.796233284331766
70-71	22.514752077853284	27.614745168541837	27.94749481627164	21.923007937333246
72-73	22.704863214089137	27.678710583283	27.785609585004718	21.830816617623146
74-75	22.444670622990127	27.772668379784303	27.850845012905257	21.93181598432031
76-77	22.51011662249496	27.50080668868362	27.90076351217899	22.08831317664243
78-79	22.44441042963602	27.60858309576116	27.77934806367804	22.167658410924783
80-81	22.504374437180154	27.611257129051385	27.75639326739342	22.127975166375037
82-83	22.545314706050334	27.522748720777486	27.898074130541513	22.03386244263067
84-85	22.56928992365347	27.501257453784184	27.83903336722894	22.090419255333405
86-87	22.43465164687894	27.530720716345662	27.897251838537485	22.137375798237912
88-89	22.572514626030838	27.538074758942262	27.762666358293153	22.126744256733748
90-91	22.570050222959008	27.601818666577127	27.756767900679698	22.071363209784167
92-93	22.623221356598506	27.53215957934331	27.79914976099972	22.04546930305846
94-95	22.421853818532895	27.634386051756426	27.79060441486131	22.153155714849373
96-97	22.5925073666405	27.723082438731794	27.806379405932237	21.878030788695472
98-99	23.65032127868381	29.59754726791805	28.034455539309388	18.717675914088755
100	30.03531354129517	40.688993039325915	29.27569341937891	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	704.0
1	691.0
2	821.5
3	1048.0
4	1380.0
5	1999.5
6	2536.5
7	3087.0
8	3958.0
9	4590.5
10	4622.5
11	4663.5
12	5277.0
13	6613.0
14	9052.5
15	11973.0
16	14674.5
17	16968.0
18	17627.0
19	16540.0
20	14904.0
21	13611.5
22	13519.0
23	15054.5
24	18364.5
25	23481.0
26	30425.0
27	38695.5
28	47264.0
29	56650.5
30	66286.0
31	75751.0
32	85905.0
33	96694.5
34	107179.5
35	116995.0
36	127441.5
37	136265.0
38	143269.0
39	149760.5
40	155945.5
41	160741.5
42	163667.5
43	167114.0
44	171542.0
45	175025.0
46	175703.5
47	175597.0
48	175420.5
49	173591.0
50	171024.0
51	165914.0
52	157951.0
53	149562.5
54	139966.5
55	129351.0
56	118040.5
57	106770.0
58	95413.5
59	81765.5
60	67669.0
61	55205.5
62	43981.5
63	33737.0
64	25062.5
65	18914.0
66	14230.5
67	10797.0
68	8312.5
69	6340.5
70	4697.0
71	3340.5
72	2320.0
73	1538.0
74	1024.5
75	632.5
76	385.5
77	250.0
78	168.5
79	108.5
80	76.0
81	52.5
82	28.0
83	15.5
84	11.0
85	9.5
86	9.0
87	8.0
88	6.0
89	4.0
90	3.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.009221831890350005
3	2.4140921178926713E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.1729580441639238E-4
26-27	2.897618797239487E-4
28-29	0.0026329195623749514
30-31	0.01228248160287352
32-33	0.0072916807978310036
34-35	7.529100885701569E-4
36-37	8.532655141649999E-5
38-39	7.96786181324999E-4
40-41	1.2336058409756244E-5
42-43	0.0015519953196787544
44-45	5.879970044680267E-4
46-47	0.0015648129102112877
48-49	0.003220766483863769
50-51	0.002169140320917251
52-53	0.0
54-55	0.0014850190883572028
56-57	0.04549944205734689
58-59	0.0630145003528759
60-61	0.043116179476538334
62-63	0.01271031258751804
64-65	0.0
66-67	0.0
68-69	2.3500791216344273E-4
70-71	0.0014376744401584066
72-73	0.005100904917441431
74-75	0.009255779850636452
76-77	0.01763782076902908
78-79	0.012203255805459813
80-81	0.005988410595299258
82-83	0.034389792695371374
84-85	0.024821733802586565
86-87	0.00761569025223317
88-89	0.0019349149460143494
90-91	0.0031266114370522243
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	26.0
20-21	131.0
22-23	308.0
24-25	381.0
26-27	1055.0
28-29	2692.0
30-31	7559.0
32-33	9827.0
34-35	13548.0
36-37	21439.0
38-39	25985.0
40-41	25611.0
42-43	28863.0
44-45	33940.0
46-47	34926.0
48-49	32892.0
50-51	29632.0
52-53	27788.0
54-55	30215.0
56-57	31511.0
58-59	31489.0
60-61	31470.0
62-63	30562.0
64-65	31699.0
66-67	33300.0
68-69	34485.0
70-71	34611.0
72-73	31801.0
74-75	32291.0
76-77	33728.0
78-79	34855.0
80-81	35034.0
82-83	32889.0
84-85	32118.0
86-87	33233.0
88-89	35125.0
90-91	35594.0
92-93	34768.0
94-95	40294.0
96-97	126451.0
98-99	960084.0
100-101	2058134.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.37912818266098
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.35575148810139	82.54676550660996
2	5.694537377187748	10.179455723532357
3	1.1525806042318583	3.0904994869946427
4	0.38459683417684737	1.374997189621522
5	0.16423191334548082	0.7339452617294702
6	0.09449258982690742	0.5067399179070459
7	0.04869519560216828	0.30466338907241636
8	0.028350944821835398	0.20271861850642997
9	0.01547895675232971	0.12451460937303084
>10	0.05992578612182794	0.8039272716303212
>50	7.977242557098307E-4	0.04690586985758512
>100	5.605855759621085E-4	0.08486715516527511
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.8281842357853427E-5	0.0	0.0	0.0
3	0.0	4.8281842357853427E-5	0.0	0.0	0.0
4	0.0	9.656368471570685E-5	0.0	0.0	0.0
5	0.0	9.656368471570685E-5	0.0	2.4140921178926713E-5	0.0
6	2.4140921178926713E-5	9.656368471570685E-5	0.0	2.4140921178926713E-5	0.0
7	2.4140921178926713E-5	1.2070460589463357E-4	0.0	2.4140921178926713E-5	0.0
8	2.4140921178926713E-5	1.2070460589463357E-4	0.0	2.4140921178926713E-5	2.4140921178926713E-5
9	2.4140921178926713E-5	1.4484552707356028E-4	0.0	2.4140921178926713E-5	2.4140921178926713E-5
10-11	2.4140921178926713E-5	1.4484552707356028E-4	0.0	2.4140921178926713E-5	2.4140921178926713E-5
12-13	2.4140921178926713E-5	1.4484552707356028E-4	0.0	2.4140921178926713E-5	2.4140921178926713E-5
14-15	2.4140921178926713E-5	2.6555013296819383E-4	0.0	2.4140921178926713E-5	2.4140921178926713E-5
16-17	9.656368471570685E-5	2.6555013296819383E-4	0.0	4.8281842357853427E-5	4.8281842357853427E-5
18-19	1.0863414530517022E-4	2.8969105414712056E-4	0.0	4.8281842357853427E-5	4.8281842357853427E-5
20-21	1.2070460589463357E-4	3.138319753260473E-4	0.0	4.8281842357853427E-5	4.8281842357853427E-5
22-23	1.2070460589463357E-4	3.37972896504974E-4	0.0	4.8281842357853427E-5	4.8281842357853427E-5
24-25	1.2070460589463357E-4	3.621138176839007E-4	0.0	4.8281842357853427E-5	4.8281842357853427E-5
26-27	1.2070460589463357E-4	3.862547388628274E-4	0.0	8.449322412624349E-5	7.242276353678014E-5
28-29	1.2070460589463357E-4	3.862547388628274E-4	0.0	3.017615147365839E-4	7.242276353678014E-5
30-31	1.2070460589463357E-4	5.06959344757461E-4	0.0	9.052845442097518E-4	7.242276353678014E-5
32-33	1.2070460589463357E-4	5.06959344757461E-4	0.0	0.002631360408503012	7.242276353678014E-5
34-35	1.2070460589463357E-4	5.673116477047779E-4	0.0	0.004562634102817148	7.242276353678014E-5
36-37	1.2070460589463357E-4	6.397344112415579E-4	0.0	0.007737165237846013	8.449322412624349E-5
38-39	1.2070460589463357E-4	6.75945793009948E-4	0.0	0.012601560855399745	9.656368471570685E-5
40-41	1.2070460589463357E-4	6.75945793009948E-4	0.0	0.017647013381795428	1.2070460589463357E-4
42-43	1.2070460589463357E-4	7.000867141888746E-4	0.0	0.02289766373821199	1.2070460589463357E-4
44-45	1.2070460589463357E-4	8.570027018518984E-4	0.0	0.028848400808817422	1.4484552707356028E-4
46-47	1.2070460589463357E-4	9.656368471570685E-4	0.0	0.035366449527127634	1.5691598766302364E-4
48-49	1.2070460589463357E-4	9.77707307746532E-4	0.0	0.04190863916661677	1.68986448252487E-4
50-51	1.2070460589463357E-4	0.0011587642165884822	0.0	0.048088714988422016	1.68986448252487E-4
52-53	1.2070460589463357E-4	0.0011587642165884822	0.0	0.05424464988904833	1.68986448252487E-4
54-55	1.2070460589463357E-4	0.0011708346771779456	0.0	0.05966428669371737	1.8105690884195034E-4
56-57	1.2070460589463357E-4	0.0012311869801252624	0.0	0.0657477988308069	2.0519783002087707E-4
58-59	1.2070460589463357E-4	0.0012432574407147258	0.0	0.0722054952461698	2.1726829061034043E-4
60-61	1.4484552707356028E-4	0.0012553279013041891	0.0	0.07845799383151182	2.1726829061034043E-4
62-63	2.6555013296819383E-4	0.0012553279013041891	0.0	0.08484326748333794	2.1726829061034043E-4
64-65	2.776205935576572E-4	0.0014243143495566762	0.0	0.09080607501453283	2.293387511998038E-4
66-67	2.8969105414712056E-4	0.0014725961919145295	0.0	0.09703443267869592	2.4140921178926713E-4
68-69	2.8969105414712056E-4	0.0014725961919145295	0.0	0.10259891501043852	2.5347967237873047E-4
70-71	2.8969105414712056E-4	0.0014725961919145295	0.0	0.10748745154917119	2.6555013296819383E-4
72-73	3.37972896504974E-4	0.0014725961919145295	0.0	0.11144656262251518	3.37972896504974E-4
74-75	3.37972896504974E-4	0.0014725961919145295	0.0	0.11435554362457584	3.37972896504974E-4
76-77	3.37972896504974E-4	0.0014967371130934562	0.0	0.11697483357248939	3.621138176839007E-4
78-79	3.37972896504974E-4	0.0014967371130934562	0.0	0.11891817772739299	3.621138176839007E-4
80-81	3.37972896504974E-4	0.0014967371130934562	0.0	0.11965447582335026	3.7418427827336405E-4
82-83	3.37972896504974E-4	0.0014967371130934562	0.0	0.12012522378633933	3.862547388628274E-4
84-85	3.37972896504974E-4	0.0014967371130934562	0.0	0.12029421023459182	3.862547388628274E-4
86-87	3.37972896504974E-4	0.001520878034272383	0.0	0.12034249207694966	4.3453658122068087E-4
88	3.621138176839007E-4	0.0015691598766302364	0.0	0.12034249207694966	4.5867750239960754E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6090	0.0	49.69068	1
GTATCAA	11090	0.0	36.870583	1
TCAACGC	12565	0.0	31.723274	4
ATCAACG	12680	0.0	31.46922	3
TATCAAC	12785	0.0	31.348076	2
CAACGCA	12915	0.0	30.863565	5
AACGCAG	13330	0.0	29.870678	6
ACGCAGA	15215	0.0	26.169973	7
CGCAGAG	15790	0.0	25.298067	8
GTGGTAT	3100	0.0	24.370117	1
TGGTATC	2875	0.0	23.60507	2
GCAGAGT	18360	0.0	21.687155	9
GAGTACT	10195	0.0	20.616388	12-13
GTACATG	13720	0.0	19.381168	1
CAGAGTA	18030	0.0	19.11351	10-11
GTACTTT	11235	0.0	18.651222	14-15
TACATGG	13800	0.0	18.371872	2
ACATGGG	13565	0.0	18.247433	3
AGAGTAC	16830	0.0	17.458761	10-11
ACTTTTT	13260	0.0	15.900128	16-17
>>END_MODULE