FastQCFastQC Report
Fri 27 May 2016
SRR2139468_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139468_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5052530
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71800.050.8353541
GTATCAA134850.039.473961
TCAACGC153800.033.8286064
TATCAAC155550.033.7257582
ATCAACG155500.033.4587753
CAACGCA159700.032.5520445
AACGCAG164600.031.5829946
ACGCAGA189800.027.4347677
CGCAGAG197050.026.4036528
GTGGTAT35450.025.2273331
TGGTATC33250.024.1939892
GCAGAGT228450.022.9430839
GTACATG172300.020.8610081
GAGTACT129900.020.8168412-13
TACATGG172550.020.012432
ACATGGG170100.019.9972233
CAGAGTA223450.019.70343210-11
GTACTTT143050.018.76910214-15
AGAGTAC208300.017.79861510-11
CATGGGG122650.017.1285574
Produced by FastQC (version 0.11.5)