FastQCFastQC Report
Fri 27 May 2016
SRR2139467_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139467_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5473629
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73800.049.8637961
GTATCAA136000.037.6178321
TCAACGC154850.032.3185964
ATCAACG156100.032.0597953
TATCAAC158750.031.7961622
CAACGCA158800.031.487795
AACGCAG164050.030.48016
TGGTATC33650.026.4190252
ACGCAGA192300.026.0690697
GTGGTAT39400.025.3838881
CGCAGAG198800.025.216718
GCAGAGT230850.021.623199
GTACATG189900.020.7512591
GAGTACT120150.020.2748612-13
TACATGG191100.019.8828952
ACATGGG190200.019.5261253
CAGAGTA225950.019.08471110-11
GTACTTT129900.018.72060414-15
AGAGTAC206650.017.2996510-11
CATGGGG135450.017.1958754
Produced by FastQC (version 0.11.5)