FastQCFastQC Report
Fri 27 May 2016
SRR2139466_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139466_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4589036
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74950.044.2100331
GTATCAA127000.034.1915471
CTTATAC38550.033.358761
TCAACGC137750.030.7747154
ATCAACG138750.030.6455923
CAACGCA139950.030.3215665
TATCAAC144400.029.7152822
AACGCAG144150.029.4678446
TATACAC57200.026.677393
ACGCAGA165400.025.733757
CGCAGAG169250.025.1483758
TTATACA57800.024.6961572
GCAGAGT196000.021.6286569
GAGTACT111500.021.43183312-13
ACACATC68850.020.295656
ACATCTC70400.019.9096878
GTACATG142500.019.7334021
CAGAGTA191250.019.56600410-11
TACACAT78850.019.5156085
GTGGTAT40450.019.2870521
Produced by FastQC (version 0.11.5)