##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2139466_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4589036 Sequences flagged as poor quality 0 Sequence length 19-100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.72638000660705 27.0 18.0 33.0 18.0 33.0 2 29.86412418643044 32.0 27.0 33.0 25.0 33.0 3 27.242036018022084 29.0 25.0 31.0 18.0 33.0 4 31.109567673907986 32.0 31.0 33.0 29.0 33.0 5 32.568406087901685 33.0 33.0 33.0 32.0 33.0 6 37.01338777904554 38.0 37.0 38.0 35.0 38.0 7 37.072073088988624 38.0 38.0 38.0 35.0 38.0 8 37.2999773808704 38.0 38.0 38.0 36.0 38.0 9 37.46338403098167 38.0 38.0 38.0 37.0 38.0 10-11 37.36091937391644 38.0 38.0 38.0 36.5 38.0 12-13 37.55699639314226 38.0 38.0 38.0 37.5 38.0 14-15 37.527966440010495 38.0 38.0 38.0 37.0 38.0 16-17 37.574913119008 38.0 38.0 38.0 38.0 38.0 18-19 37.57141194795595 38.0 38.0 38.0 38.0 38.0 20-21 37.577416241663 38.0 38.0 38.0 38.0 38.0 22-23 37.58336618303615 38.0 38.0 38.0 38.0 38.0 24-25 37.58572521859663 38.0 38.0 38.0 38.0 38.0 26-27 37.555840500938345 38.0 38.0 38.0 38.0 38.0 28-29 37.53191217275905 38.0 38.0 38.0 38.0 38.0 30-31 37.52742743067842 38.0 38.0 38.0 38.0 38.0 32-33 37.44011045646813 38.0 38.0 38.0 37.0 38.0 34-35 37.47669449828857 38.0 38.0 38.0 37.0 38.0 36-37 37.459914176865595 38.0 38.0 38.0 37.0 38.0 38-39 37.43613661327136 38.0 38.0 38.0 37.0 38.0 40-41 37.447069801267176 38.0 38.0 38.0 37.0 38.0 42-43 37.413666048450594 38.0 38.0 38.0 37.0 38.0 44-45 37.377432940670744 38.0 38.0 38.0 37.0 38.0 46-47 37.348859282604906 38.0 38.0 38.0 37.0 38.0 48-49 37.23835277396165 38.0 38.0 38.0 37.0 38.0 50-51 37.226972127601776 38.0 38.0 38.0 37.0 38.0 52-53 37.23308829702481 38.0 38.0 38.0 37.0 38.0 54-55 37.213342722655604 38.0 38.0 38.0 37.0 38.0 56-57 37.159744999159834 38.0 38.0 38.0 36.5 38.0 58-59 37.13805450975719 38.0 38.0 38.0 36.0 38.0 60-61 37.14682679087852 38.0 38.0 38.0 36.0 38.0 62-63 37.150289200638866 38.0 38.0 38.0 36.0 38.0 64-65 37.15546956500003 38.0 38.0 38.0 36.0 38.0 66-67 37.091610752674455 38.0 38.0 38.0 36.0 38.0 68-69 37.104900280300456 38.0 38.0 38.0 36.0 38.0 70-71 37.11757491758193 38.0 38.0 38.0 36.0 38.0 72-73 37.13446425862942 38.0 38.0 38.0 36.0 38.0 74-75 37.11876963895525 38.0 38.0 38.0 36.0 38.0 76-77 37.08990353012899 38.0 38.0 38.0 36.0 38.0 78-79 37.08791768813356 38.0 38.0 38.0 36.0 38.0 80-81 37.08813032838759 38.0 38.0 38.0 36.0 38.0 82-83 37.0291564564338 38.0 38.0 38.0 36.0 38.0 84-85 37.027483642012356 38.0 38.0 38.0 36.0 38.0 86-87 37.02230936666961 38.0 38.0 38.0 36.0 38.0 88-89 36.99777783471022 38.0 38.0 38.0 36.0 38.0 90-91 36.9738235668821 38.0 38.0 38.0 36.0 38.0 92-93 36.957722706633604 38.0 38.0 38.0 35.0 38.0 94-95 36.95067622039221 38.0 38.0 38.0 35.0 38.0 96-97 36.93054317538442 38.0 38.0 38.0 35.0 38.0 98-99 36.95344349892157 38.0 38.0 38.0 35.0 38.0 100 35.499979129001034 38.0 35.0 38.0 29.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 4.0 20 18.0 21 78.0 22 215.0 23 648.0 24 1517.0 25 3115.0 26 5861.0 27 10018.0 28 16286.0 29 23881.0 30 31550.0 31 40244.0 32 51802.0 33 70079.0 34 109249.0 35 220340.0 36 824445.0 37 3179686.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.965607591659776 17.624878078969093 12.247910018574707 22.161604310796427 2 14.671187132146468 20.059983267274884 42.198590292970884 23.070239307607768 3 19.44860290858599 25.496128379088063 29.824664624406793 25.230604087919158 4 12.945965122086642 17.90452286711196 39.041925145063146 30.107586865738252 5 13.770669918475253 37.4666923510733 35.0169839591583 13.745653771293142 6 30.010180787424634 37.458237416311405 18.801399683942336 13.730182112321629 7 26.932889609059508 32.660672088865724 22.507864396792705 17.898573905282067 8 24.07749688605624 37.09391253413571 20.94982475622331 17.878765823584736 9 25.38613774221863 17.822980687011388 21.43308529285889 35.35779627791109 10-11 24.447541923837598 26.953885304015916 28.351553572471428 20.24701919967505 12-13 25.370949367143776 24.2444164743968 28.720105922028065 21.664528236431355 14-15 22.45712389268683 25.71699154245031 26.398158567507423 25.427725997355438 16-17 21.955319156354406 29.01728162516049 27.07267931652748 21.954719901957624 18-19 21.941623469504272 27.937218187000497 28.918143156863447 21.20301518663179 20-21 22.602844056729467 27.034130772740884 28.97947193882332 21.383553231706333 22-23 22.410675973089305 27.074206211444345 28.818277845444605 21.696839970021742 24-25 22.30597345506437 27.234892549077728 28.922853610548234 21.536280385309674 26-27 22.22885954082398 27.32308330804831 28.95748759290066 21.490569558227048 28-29 22.04754352494459 27.221454146187817 29.097271351287233 21.633730977580356 30-31 22.319019010127196 27.37299209200883 28.811350334288484 21.496638563575488 32-33 22.318324291629995 27.249762975719726 28.82686399796585 21.605048734684427 34-35 22.325651468385583 27.21937526091058 28.880667580470025 21.574305690233807 36-37 22.149132114840704 27.320420926886264 28.85348421876041 21.67696273951262 38-39 22.036852234538582 27.217030248539274 29.021911311825416 21.724206205096724 40-41 22.28824386808457 27.09237876649863 28.938361629462957 21.681015735953846 42-43 22.2903596998038 27.153098673513703 28.760322516482358 21.79621911020014 44-45 22.182549893401816 27.36912262328414 28.666889581768306 21.78143790154573 46-47 22.483254941737307 27.223131521826787 28.246792115937026 22.046821420498883 48-49 22.343573194848933 27.282084439434662 28.255132957187307 22.119209408529102 50-51 22.192189769708754 27.46331795781016 28.316375018739027 22.028117253742064 52-53 22.374786683767606 27.44605324875218 28.336065981482456 21.843094085997762 54-55 22.365672436532837 27.414993850183205 28.16858338882407 22.050750324459887 56-57 22.459437930666727 27.666193352077155 27.946182762665998 21.928185954590113 58-59 22.28255937524345 27.839119955326957 28.165794555145812 21.71252611428378 60-61 22.452105718063585 28.089263601064996 27.844232846348692 21.614397834522723 62-63 22.275486399373168 28.27110094364251 27.8064494540046 21.646963202979723 64-65 22.246882009777018 28.1773444128633 27.807914435949783 21.767859141409904 66-67 22.425086917869667 28.165249100647248 27.675605930043634 21.73405805143945 68-69 22.273548269502804 28.002570661849823 27.899466063700118 21.824415004947255 70-71 22.310122928228107 27.74110971768578 28.058383126812974 21.890384227273138 72-73 22.503909645772957 27.599275048038415 27.976664863044675 21.920150443143957 74-75 22.290216988051 27.71707609302411 27.988706545498644 22.00400037342624 76-77 22.370994118679945 27.545480774237497 27.96475368394885 22.11877142313371 78-79 22.303731211012988 27.566558247408494 27.95443130623361 22.175279235344913 80-81 22.340388404929055 27.659473314561424 27.902201702272585 22.09793657823694 82-83 22.421769568880368 27.55532612827633 28.00189352877629 22.02101077406701 84-85 22.464780036293742 27.524884837161355 27.969322123076594 22.041013003468308 86-87 22.306099926915515 27.618242190822563 27.988906219440906 22.086751662821015 88-89 22.42030351059771 27.547080792568266 27.908273993733957 22.124341703100065 90-91 22.392153389583328 27.655949765466897 27.86925337243669 22.082643472513087 92-93 22.468503620975483 27.552234534508337 27.946210627344907 22.033051217171277 94-95 22.31633173336975 27.661645371284006 27.93049117123147 22.091531724114773 96-97 22.478122086703987 27.718940236887214 27.93778381141819 21.865153864990614 98-99 23.469771555939065 29.710553870840528 28.135527322671212 18.6841472505492 100 22.500991372450805 30.563523494222643 22.08145168233296 24.85403345099359 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 639.0 1 651.0 2 841.5 3 1129.0 4 1512.5 5 2202.5 6 2839.5 7 3505.5 8 4601.0 9 5402.5 10 5396.5 11 5248.5 12 5675.0 13 7030.5 14 9676.0 15 12757.5 16 15475.5 17 17932.5 18 18861.0 19 17839.5 20 16058.0 21 14520.0 22 14161.5 23 15656.0 24 19321.5 25 25083.0 26 32536.0 27 41029.5 28 50143.5 29 60210.0 30 70191.5 31 80490.0 32 92352.5 33 103851.0 34 114993.5 35 125849.5 36 137765.5 37 148772.0 38 156710.0 39 163705.0 40 169957.0 41 175912.5 42 181538.0 43 191032.5 44 198810.0 45 202174.0 46 202068.5 47 198051.5 48 194820.0 49 191006.0 50 187303.5 51 181417.0 52 173483.0 53 164423.0 54 153537.0 55 142396.5 56 131136.0 57 118650.0 58 105636.5 59 91753.0 60 76307.5 61 61597.5 62 48431.5 63 37543.0 64 28150.0 65 20568.0 66 15307.0 67 11610.5 68 8882.0 69 6527.0 70 4724.5 71 3398.0 72 2329.0 73 1526.0 74 948.5 75 581.0 76 372.0 77 235.0 78 158.0 79 107.0 80 73.0 81 50.0 82 31.0 83 18.5 84 11.0 85 5.0 86 4.0 87 5.0 88 6.0 89 5.5 90 2.5 91 1.0 92 1.0 93 0.5 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.009217622175986416 3 4.358213794792632E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.160231128407383E-4 26-27 2.942644908096295E-4 28-29 0.0025729067468483256 30-31 0.012471650998905048 32-33 0.007312701386221471 34-35 6.135812259922649E-4 36-37 1.2095332773942518E-4 38-39 6.964592014199808E-4 40-41 0.0 42-43 0.001521407660063832 44-45 6.083562462076311E-4 46-47 0.0014197745057339013 48-49 0.002885771873549599 50-51 0.002019561822018671 52-53 0.0 54-55 0.001544744579994481 56-57 0.04670796133132666 58-59 0.06414956011730205 60-61 0.04433521738036381 62-63 0.012767436731130346 64-65 0.0 66-67 0.0 68-69 2.3590832652096308E-4 70-71 0.001480980569785938 72-73 0.005152940152153884 74-75 0.008925063781553366 76-77 0.017413912553480283 78-79 0.012414569958383805 80-81 0.006189315897547596 82-83 0.035594073327514006 84-85 0.02489206171352659 86-87 0.007502921941171132 88-89 0.00231107982264582 90-91 0.0032330243768656382 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 37.0 20-21 239.0 22-23 393.0 24-25 481.0 26-27 1118.0 28-29 2624.0 30-31 7642.0 32-33 10013.0 34-35 14129.0 36-37 22738.0 38-39 27078.0 40-41 26023.0 42-43 29793.0 44-45 35403.0 46-47 36239.0 48-49 34524.0 50-51 31093.0 52-53 29238.0 54-55 31855.0 56-57 33439.0 58-59 33732.0 60-61 33506.0 62-63 33410.0 64-65 36411.0 66-67 40272.0 68-69 41980.0 70-71 46724.0 72-73 35952.0 74-75 33798.0 76-77 35641.0 78-79 37276.0 80-81 36962.0 82-83 34751.0 84-85 33918.0 86-87 35139.0 88-89 37071.0 90-91 37645.0 92-93 36944.0 94-95 42501.0 96-97 138547.0 98-99 306301.0 100-101 3066456.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.6426019738725 #Duplication Level Percentage of deduplicated Percentage of total 1 91.90841208835374 81.47000790798593 2 5.949064169120615 10.546810545207705 3 1.2625022607133674 3.3573445616258772 4 0.4323314322494389 1.5329193227872495 5 0.18074155528121283 0.8010700872465611 6 0.09712865512228022 0.5165842029757092 7 0.050685138466058266 0.31450037885261917 8 0.030849576835324008 0.21876694083808185 9 0.022074367960144028 0.1761056471624266 >10 0.06398906996904073 0.8768756887545908 >50 0.0016226517972301843 0.0927650416441519 >100 5.642202810705383E-4 0.07906070900044257 >500 3.4813850472333854E-5 0.01718896591862531 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 4.358213794792632E-5 0.0 0.0 0.0 3 0.0 4.358213794792632E-5 0.0 0.0 0.0 4 0.0 6.537320692188948E-5 0.0 0.0 0.0 5 0.0 6.537320692188948E-5 0.0 0.0 0.0 6 6.537320692188948E-5 6.537320692188948E-5 0.0 2.179106897396316E-5 2.179106897396316E-5 7 6.537320692188948E-5 6.537320692188948E-5 0.0 2.179106897396316E-5 4.358213794792632E-5 8 6.537320692188948E-5 1.089553448698158E-4 0.0 2.179106897396316E-5 4.358213794792632E-5 9 6.537320692188948E-5 1.089553448698158E-4 0.0 4.358213794792632E-5 4.358213794792632E-5 10-11 6.537320692188948E-5 1.089553448698158E-4 0.0 5.44776724349079E-5 6.537320692188948E-5 12-13 6.537320692188948E-5 1.089553448698158E-4 0.0 7.626874140887106E-5 6.537320692188948E-5 14-15 6.537320692188948E-5 1.5253748281774212E-4 0.0 8.716427589585264E-5 9.805981038283422E-5 16-17 1.089553448698158E-4 1.5253748281774212E-4 0.0 1.3074641384377896E-4 1.089553448698158E-4 18-19 1.089553448698158E-4 1.5253748281774212E-4 0.0 1.8522408627868686E-4 1.3074641384377896E-4 20-21 1.089553448698158E-4 1.5253748281774212E-4 0.0 2.0701515525265002E-4 1.5253748281774212E-4 22-23 1.3074641384377896E-4 2.2880622422661318E-4 0.0 2.614928276875579E-4 1.7432855179170528E-4 24-25 1.5253748281774212E-4 2.723883621745395E-4 0.0 3.0507496563548424E-4 1.9611962076566844E-4 26-27 1.5253748281774212E-4 3.0507496563548424E-4 0.0 4.1403031050530004E-4 1.9611962076566844E-4 28-29 1.5253748281774212E-4 3.0507496563548424E-4 0.0 6.755231381928579E-4 1.9611962076566844E-4 30-31 1.5253748281774212E-4 3.268660346094474E-4 0.0 0.0016234346385602554 1.9611962076566844E-4 32-33 1.5253748281774212E-4 3.268660346094474E-4 0.0 0.0037371683290346822 1.9611962076566844E-4 34-35 1.5253748281774212E-4 3.8134370704435527E-4 0.0 0.006134185916170629 2.179106897396316E-4 36-37 1.5253748281774212E-4 4.1403031050530004E-4 0.0 0.010078369400457962 2.179106897396316E-4 38-39 1.5253748281774212E-4 4.358213794792632E-4 0.0 0.015635091988818565 2.179106897396316E-4 40-41 1.5253748281774212E-4 4.794035174271895E-4 0.0 0.022139726077546572 2.3970175871359476E-4 42-43 1.5253748281774212E-4 4.794035174271895E-4 0.0 0.0284482405455089 2.614928276875579E-4 44-45 1.5253748281774212E-4 5.338811898620973E-4 0.0 0.035290636203333337 2.614928276875579E-4 46-47 1.5253748281774212E-4 5.883588622970053E-4 0.0 0.042318255947436455 2.832838966615211E-4 48-49 1.5253748281774212E-4 5.883588622970053E-4 0.0 0.04947662210538335 3.268660346094474E-4 50-51 1.7432855179170528E-4 7.191052761407842E-4 0.0 0.056678570401278175 3.268660346094474E-4 52-53 1.7432855179170528E-4 7.191052761407842E-4 0.0 0.06409842938691263 3.5955263807039217E-4 54-55 1.7432855179170528E-4 7.191052761407842E-4 0.0 0.0699711224753957 3.922392415313369E-4 56-57 1.7432855179170528E-4 7.191052761407842E-4 0.0 0.07534262097747763 3.922392415313369E-4 58-59 1.7432855179170528E-4 7.191052761407842E-4 0.0 0.08058337306571577 3.922392415313369E-4 60-61 1.8522408627868686E-4 7.191052761407842E-4 0.0 0.08616188672305033 3.922392415313369E-4 62-63 1.9611962076566844E-4 7.626874140887105E-4 0.0 0.0909123397593743 4.1403031050530004E-4 64-65 1.9611962076566844E-4 8.716427589585264E-4 0.0 0.09576085260608111 4.1403031050530004E-4 66-67 1.9611962076566844E-4 8.716427589585264E-4 0.0 0.10113235110816302 4.249258449922816E-4 68-69 1.9611962076566844E-4 9.152248969064527E-4 0.0 0.1059372818169219 4.467169139662448E-4 70-71 1.9611962076566844E-4 9.261204313934343E-4 0.0 0.11042624202555831 4.794035174271895E-4 72-73 2.0701515525265002E-4 9.370159658804158E-4 0.0 0.11409803714767111 5.011945864011526E-4 74-75 2.179106897396316E-4 9.697025693413605E-4 0.0 0.11735580195927858 5.011945864011526E-4 76-77 2.2880622422661318E-4 0.0010023891728023052 0.0 0.12010147664999796 5.011945864011526E-4 78-79 2.3970175871359476E-4 0.0010241802417762685 0.0 0.12216073266803747 5.011945864011526E-4 80-81 2.3970175871359476E-4 0.0010241802417762685 0.0 0.12279267366828239 5.120901208881342E-4 82-83 2.3970175871359476E-4 0.0010241802417762685 0.0 0.1232720771857096 5.229856553751158E-4 84-85 2.3970175871359476E-4 0.0010459713107502317 0.0 0.12352267447891016 5.229856553751158E-4 86-87 2.3970175871359476E-4 0.0010677623797241949 0.0 0.12359894322031904 5.338811898620973E-4 88 2.3970175871359476E-4 0.001220299862541937 0.0 0.12359894322031904 5.447767243490789E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7495 0.0 44.210033 1 GTATCAA 12700 0.0 34.191547 1 CTTATAC 3855 0.0 33.35876 1 TCAACGC 13775 0.0 30.774715 4 ATCAACG 13875 0.0 30.645592 3 CAACGCA 13995 0.0 30.321566 5 TATCAAC 14440 0.0 29.715282 2 AACGCAG 14415 0.0 29.467844 6 TATACAC 5720 0.0 26.67739 3 ACGCAGA 16540 0.0 25.73375 7 CGCAGAG 16925 0.0 25.148375 8 TTATACA 5780 0.0 24.696157 2 GCAGAGT 19600 0.0 21.628656 9 GAGTACT 11150 0.0 21.431833 12-13 ACACATC 6885 0.0 20.29565 6 ACATCTC 7040 0.0 19.909687 8 GTACATG 14250 0.0 19.733402 1 CAGAGTA 19125 0.0 19.566004 10-11 TACACAT 7885 0.0 19.515608 5 GTGGTAT 4045 0.0 19.287052 1 >>END_MODULE