FastQCFastQC Report
Fri 27 May 2016
SRR2139465_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139465_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3711810
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50800.051.589081
GTATCAA93000.036.381781
TCAACGC108300.030.844344
ATCAACG110000.030.4838583
CAACGCA111450.030.010795
TATCAAC113700.029.6457482
AACGCAG115550.029.019686
ACGCAGA130800.025.5711217
CGCAGAG135700.024.6477728
GCAGAGT155750.021.5021749
GTACATG112050.021.2211381
TGGTATC22500.021.022622
GAGTACT94150.020.81734712-13
TACATGG111250.020.3395292
ACATGGG109800.020.1396963
GTGGTAT24850.019.8919771
CAGAGTA153350.019.39364810-11
GTACTTT104300.018.66919314-15
TATACCG2554.157664E-818.3797825
CATGGGG79250.018.1183414
Produced by FastQC (version 0.11.5)