##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139465_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3711810
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.261916423523832	32.0	25.0	33.0	18.0	34.0
2	31.618729406947015	33.0	32.0	33.0	28.0	33.0
3	32.53346696086276	33.0	33.0	33.0	31.0	34.0
4	32.696622133137204	33.0	33.0	34.0	31.0	34.0
5	33.19153512706739	33.0	33.0	34.0	33.0	34.0
6	37.08245492091459	38.0	38.0	38.0	36.0	38.0
7	37.22036796064454	38.0	38.0	38.0	36.0	38.0
8	37.16771979169192	38.0	38.0	38.0	36.0	38.0
9	37.3676114887346	38.0	38.0	38.0	37.0	38.0
10-11	37.426485865386425	38.0	38.0	38.0	37.0	38.0
12-13	37.536861127051225	38.0	38.0	38.0	37.5	38.0
14-15	37.52066970022712	38.0	38.0	38.0	37.5	38.0
16-17	37.53894259135031	38.0	38.0	38.0	38.0	38.0
18-19	37.543373852648706	38.0	38.0	38.0	38.0	38.0
20-21	37.54900958650464	38.0	38.0	38.0	38.0	38.0
22-23	37.55797737318139	38.0	38.0	38.0	38.0	38.0
24-25	37.56534737879864	38.0	38.0	38.0	38.0	38.0
26-27	37.537126919474915	38.0	38.0	38.0	38.0	38.0
28-29	37.514241280896485	38.0	38.0	38.0	38.0	38.0
30-31	37.510445372982886	38.0	38.0	38.0	38.0	38.0
32-33	37.42228059753022	38.0	38.0	38.0	37.0	38.0
34-35	37.46921712470443	38.0	38.0	38.0	37.0	38.0
36-37	37.45528171335931	38.0	38.0	38.0	37.0	38.0
38-39	37.44547327137506	38.0	38.0	38.0	37.0	38.0
40-41	37.431845290060075	38.0	38.0	38.0	37.0	38.0
42-43	37.39516459000779	38.0	38.0	38.0	37.0	38.0
44-45	37.356692830249806	38.0	38.0	38.0	37.0	38.0
46-47	37.32512507292113	38.0	38.0	38.0	37.0	38.0
48-49	37.205518155121894	38.0	38.0	38.0	37.0	38.0
50-51	37.19875062140295	38.0	38.0	38.0	37.0	38.0
52-53	37.20501539168518	38.0	38.0	38.0	37.0	38.0
54-55	37.188628160573046	38.0	38.0	38.0	37.0	38.0
56-57	37.1361636512337	38.0	38.0	38.0	36.5	38.0
58-59	37.11966556920618	38.0	38.0	38.0	36.0	38.0
60-61	37.13208126603199	38.0	38.0	38.0	36.0	38.0
62-63	37.13503255298191	38.0	38.0	38.0	36.0	38.0
64-65	37.14153702241653	38.0	38.0	38.0	36.0	38.0
66-67	37.075290108691	38.0	38.0	38.0	36.0	38.0
68-69	37.09444746901505	38.0	38.0	38.0	36.0	38.0
70-71	37.1110215595131	38.0	38.0	38.0	36.0	38.0
72-73	37.1254999298034	38.0	38.0	38.0	36.0	38.0
74-75	37.112399368525956	38.0	38.0	38.0	36.0	38.0
76-77	37.07609785278109	38.0	38.0	38.0	36.0	38.0
78-79	37.07546266678313	38.0	38.0	38.0	36.0	38.0
80-81	37.07125522905329	38.0	38.0	38.0	36.0	38.0
82-83	37.00878690096917	38.0	38.0	38.0	36.0	38.0
84-85	37.00703051276243	38.0	38.0	38.0	36.0	38.0
86-87	37.00007866242552	38.0	38.0	38.0	36.0	38.0
88-89	36.97947796416561	38.0	38.0	38.0	36.0	38.0
90-91	36.95557365373584	38.0	38.0	38.0	35.5	38.0
92-93	36.940193415487926	38.0	38.0	38.0	35.0	38.0
94-95	36.934919939262095	38.0	38.0	38.0	35.0	38.0
96-97	36.905296361370134	38.0	38.0	38.0	35.0	38.0
98-99	36.92745662262888	38.0	38.0	38.0	35.0	38.0
100	35.53992690587393	38.0	36.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	6.0
21	33.0
22	181.0
23	508.0
24	1254.0
25	2598.0
26	4861.0
27	8181.0
28	13278.0
29	19705.0
30	26463.0
31	33567.0
32	43045.0
33	56082.0
34	82423.0
35	149284.0
36	459874.0
37	2810466.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.05107750666117	19.45191160107872	14.58595671653452	24.91105417572559
2	14.249759321914249	20.12591512234368	43.22136537021584	22.402960185526226
3	18.339472300291394	25.855028061796297	30.109666232736178	25.695833405176128
4	12.571090653885841	17.971394009930467	38.76795956689594	30.689555769287757
5	13.692107085222574	37.05960164986893	35.49397733181386	13.754313933094636
6	30.100543939479664	37.49577699289565	18.82181469417885	13.581864373445839
7	26.79808503129201	32.38285364822014	22.9644836346688	17.85457768581905
8	24.18499330515301	36.8679431328651	20.982297046454427	17.964766515527465
9	25.287716774296097	17.559546420748905	21.648979877741585	35.50375692721341
10-11	24.330784711501934	26.940360632683248	28.546841029039744	20.182013626775078
12-13	25.127067387608744	24.2511470145293	28.983056783617695	21.638728814244264
14-15	22.228562345594195	25.569061994013705	26.903060770890754	25.29931488950135
16-17	21.666639725632507	28.802942499750795	27.540297051842632	21.990120722774066
18-19	21.65781653694559	27.78880115092098	29.440151839668516	21.113230472464917
20-21	22.438588465532003	26.910251788773092	29.44565141838297	21.205508327311932
22-23	22.236593220998856	26.743816522655067	29.292337503497514	21.727252752848564
24-25	21.96088126285781	26.90772553293823	29.392740750121586	21.73865245408237
26-27	21.871876949958843	27.036496425111945	29.36421926662751	21.7274073583017
28-29	21.834434840181764	27.06341131764589	29.37741139959843	21.724742442573913
30-31	22.082027526392324	26.98152942154417	29.266196774085596	21.670246277977913
32-33	21.87583850501588	27.10359269645902	29.24207802099833	21.77849077752677
34-35	21.998353127139207	27.10947771126719	29.2642607023339	21.627908459259697
36-37	21.97695828492508	27.03037797463006	29.206750334107184	21.785913406337677
38-39	21.82253861308878	27.14889175229112	29.31578715754502	21.712782477075073
40-41	22.111980598529513	27.030390860723518	29.136054495956436	21.72157404479053
42-43	21.963940920938317	27.117150304083403	28.970425716768027	21.948483058210254
44-45	22.0089994057102	27.246442774068	28.91080454171372	21.833753278508077
46-47	22.07538224487377	27.230564427684286	28.628246475420944	22.065806852021
48-49	22.030981165403084	27.312428081782603	28.601699300520618	22.054891452293695
50-51	22.060450042257152	27.455298845163178	28.448032342917863	22.036218769661815
52-53	22.171453535604215	27.40664920238029	28.337223660012956	22.08467360200254
54-55	22.164091449233922	27.284685732203005	28.36783020586788	22.1833926126952
56-57	22.112372303029222	27.442177173410066	28.38175344847932	22.063697075081393
58-59	22.059620797524182	27.38851279323637	28.45841557459434	22.093450834645108
60-61	22.11889386354494	27.36788093385039	28.479245896408134	22.03397930619653
62-63	22.03330040280748	27.405647398936456	28.535800696524415	22.025251501731645
64-65	21.944942987453768	27.377943577585235	28.495402272950116	22.18171116201088
66-67	22.022339520150425	27.410154941823844	28.300694058259108	22.26681147976662
68-69	22.017926086489283	27.46638761862863	28.439995651763304	22.075690643118783
70-71	22.10575541584479	27.219071276025964	28.509690209810902	22.165483098318344
72-73	22.105606037665236	27.090785928846405	28.46662053788603	22.33698749560233
74-75	22.019314659529652	27.335159687445632	28.4976145714545	22.147911081570225
76-77	22.163626100950676	27.382956769292498	28.20487440112935	22.248542728627477
78-79	22.16989877085746	27.355054949643776	28.099215316129637	22.375830963369125
80-81	22.171380055506848	27.407379328893246	28.136634863373505	22.2846057522264
82-83	22.274326472126944	27.39336043513173	28.15315345183242	22.179159640908907
84-85	22.304513846831288	27.400952812429523	28.0894799148505	22.205053425888686
86-87	22.190475241717085	27.459732955026094	28.098531656187742	22.25126014706908
88-89	22.316942044904653	27.441684959475047	28.040036270742885	22.201336724877414
90-91	22.31992686566804	27.537415702034796	27.981617866223857	22.161039566073306
92-93	22.376355187536888	27.490472119742254	28.021543716822173	22.111628975898682
94-95	22.24960817094885	27.611758162071443	28.017321245976788	22.121312421002916
96-97	22.35458993635688	27.661751033662398	28.076252380340588	21.907406649640134
98-99	23.40821371775503	29.5387939260765	28.342808602016913	18.71018375415156
100	29.744735495520157	40.59657322395289	29.658691280526956	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	635.0
1	601.0
2	718.5
3	962.0
4	1280.5
5	1843.5
6	2379.5
7	3139.5
8	4064.0
9	4614.5
10	4662.5
11	4563.0
12	5046.5
13	6436.5
14	8856.5
15	11754.5
16	14078.5
17	15325.0
18	15195.0
19	13990.0
20	12739.0
21	11706.0
22	11612.0
23	13251.5
24	16531.0
25	21535.5
26	28294.0
27	36000.0
28	44082.0
29	52814.0
30	61126.0
31	69375.0
32	78937.0
33	88711.0
34	97956.5
35	106784.5
36	116729.5
37	125146.0
38	131280.5
39	137273.5
40	143104.0
41	147918.0
42	150653.0
43	153128.0
44	156347.5
45	158701.0
46	158928.5
47	159100.5
48	158212.0
49	155561.5
50	152330.0
51	147623.0
52	140490.5
53	131888.0
54	122782.0
55	113218.5
56	103224.0
57	93021.0
58	82665.5
59	70873.0
60	58917.5
61	47657.0
62	37405.0
63	28943.0
64	21992.5
65	16442.5
66	12130.5
67	9310.0
68	7155.5
69	5331.0
70	4001.0
71	2907.5
72	2077.0
73	1418.5
74	932.0
75	610.0
76	403.0
77	271.0
78	180.0
79	112.0
80	66.0
81	34.0
82	20.5
83	13.5
84	10.5
85	10.0
86	8.5
87	9.0
88	8.0
89	3.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.011342175380744165
3	5.388206831707441E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.2333883997341076E-4
26-27	4.042221815305226E-4
28-29	0.002655299647869526
30-31	0.012480750971508402
32-33	0.007343141769291467
34-35	6.374776069036384E-4
36-37	6.810864473059761E-5
38-39	9.185103133628171E-4
40-41	2.7609097694654144E-5
42-43	0.0015707833190595928
44-45	7.146560542578087E-4
46-47	0.0014426528518983897
48-49	0.0029547959049669122
50-51	0.001828470202486518
52-53	0.0
54-55	0.0014026386848041486
56-57	0.048268880340149825
58-59	0.06859357195087097
60-61	0.046525543676509254
62-63	0.014117561083365029
64-65	0.0
66-67	0.0
68-69	2.791299643070312E-4
70-71	0.0018004088650253828
72-73	0.006195075989244589
74-75	0.009806429063002877
76-77	0.018795295576137763
78-79	0.013516839593942441
80-81	0.006579785062542416
82-83	0.03817813413648816
84-85	0.027329505864470405
86-87	0.008762389326295509
88-89	0.0028133246557897284
90-91	0.0031700646658734616
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	29.0
20-21	137.0
22-23	308.0
24-25	355.0
26-27	951.0
28-29	2396.0
30-31	8026.0
32-33	10136.0
34-35	13892.0
36-37	22039.0
38-39	25389.0
40-41	24835.0
42-43	27272.0
44-45	32451.0
46-47	32907.0
48-49	30844.0
50-51	26554.0
52-53	24539.0
54-55	26845.0
56-57	27120.0
58-59	28514.0
60-61	28119.0
62-63	27110.0
64-65	28740.0
66-67	30387.0
68-69	30434.0
70-71	30582.0
72-73	28313.0
74-75	28201.0
76-77	29151.0
78-79	30352.0
80-81	30330.0
82-83	28169.0
84-85	27760.0
86-87	28809.0
88-89	29904.0
90-91	30674.0
92-93	29693.0
94-95	35357.0
96-97	112603.0
98-99	860669.0
100-101	1840914.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.95010282503266
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.91067818461617	83.57325056249742
2	5.309570985513585	9.551929122075139
3	1.0493633326579472	2.831710190202039
4	0.3476413085695175	1.2508148580822802
5	0.148960870964717	0.6699522830091358
6	0.07245673818213412	0.39104946299096605
7	0.04355877085677107	0.27426811422490155
8	0.0339496808481321	0.24430218265332196
9	0.018260249335401973	0.14782601747971275
>10	0.0638845311962765	0.8820245714726384
>50	9.002684907272305E-4	0.05094298863258346
>100	7.346135891659217E-4	0.10827064085435932
>500	4.046517942464911E-5	0.02365900582552379
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.694103415853721E-5	0.0	0.0	0.0
6	0.0	2.694103415853721E-5	0.0	0.0	0.0
7	0.0	2.694103415853721E-5	0.0	0.0	0.0
8	0.0	5.388206831707442E-5	0.0	0.0	0.0
9	0.0	1.3470517079268606E-4	0.0	2.694103415853721E-5	0.0
10-11	0.0	1.3470517079268606E-4	0.0	5.388206831707442E-5	0.0
12-13	0.0	1.3470517079268606E-4	0.0	5.388206831707442E-5	0.0
14-15	0.0	1.4817568787195467E-4	0.0	6.735258539634301E-5	0.0
16-17	0.0	1.7511672203049185E-4	0.0	9.429361955488023E-5	0.0
18-19	0.0	2.0205775618902907E-4	0.0	1.0776413663414884E-4	0.0
20-21	0.0	2.1552827326829768E-4	0.0	1.3470517079268606E-4	0.0
22-23	0.0	2.828808586646407E-4	0.0	1.6164620495122325E-4	0.0
24-25	0.0	3.502334440609837E-4	0.0	2.289987903475663E-4	0.0
26-27	0.0	3.771744782195209E-4	0.0	3.502334440609837E-4	0.0
28-29	0.0	3.771744782195209E-4	0.0	6.46584819804893E-4	0.0
30-31	0.0	4.0411551237805814E-4	0.0	0.0019532249764939477	0.0
32-33	0.0	4.175860294573267E-4	0.0	0.0048089745972988915	0.0
34-35	0.0	4.7146809777440116E-4	0.0	0.00839213214038434	0.0
36-37	0.0	5.253501660914756E-4	0.0	0.014224866035707648	0.0
38-39	0.0	5.657617173292814E-4	0.0	0.03141324582885439	0.0
40-41	0.0	5.927027514878187E-4	0.0	0.04989479526161091	0.0
42-43	0.0	6.061732685670872E-4	0.0	0.06042873961759896	0.0
44-45	0.0	7.139374052012361E-4	0.0	0.07265996912557485	0.0
46-47	0.0	7.812899905975791E-4	0.0	0.08607660413652637	0.0
48-49	0.0	7.812899905975791E-4	0.0	0.09838865674697789	0.0
50-51	0.0	9.968182638658769E-4	0.0	0.11025618229381354	0.0
52-53	0.0	0.0010102887809451454	0.0	0.14739439788135705	0.0
54-55	1.3470517079268605E-5	0.0010372298151036827	0.0	0.1973296046942058	0.0
56-57	2.694103415853721E-5	0.0010507003321829512	0.0	0.2627963176994512	0.0
58-59	2.694103415853721E-5	0.0010507003321829512	0.0	0.3022649327417082	0.0
60-61	2.694103415853721E-5	0.0010641708492622197	0.0	0.31670532705068416	2.694103415853721E-5
62-63	2.694103415853721E-5	0.0011180529175792943	0.0	0.3293002605198003	2.694103415853721E-5
64-65	2.694103415853721E-5	0.0014278748104024721	0.0	0.33608940112775165	4.041155123780581E-5
66-67	2.694103415853721E-5	0.0014278748104024721	0.0	0.3418682529547579	5.388206831707442E-5
68-69	2.694103415853721E-5	0.0014548158445610092	0.0	0.34749892909389224	5.388206831707442E-5
70-71	2.694103415853721E-5	0.0014817568787195464	0.0	0.35236178575950816	5.388206831707442E-5
72-73	2.694103415853721E-5	0.0014817568787195464	0.0	0.3566454101907156	8.082310247561162E-5
74-75	2.694103415853721E-5	0.0015086979128780837	0.0	0.3599726279092949	8.082310247561162E-5
76-77	2.694103415853721E-5	0.0015760504982744268	0.0	0.36289573011549625	8.082310247561162E-5
78-79	2.694103415853721E-5	0.0016029915324329638	0.0	0.36482201405783166	8.082310247561162E-5
80-81	2.694103415853721E-5	0.0016164620495122326	0.0	0.36557636301427066	8.082310247561162E-5
82-83	2.694103415853721E-5	0.0016299325665915013	0.0	0.36619600679991704	8.082310247561162E-5
84-85	2.694103415853721E-5	0.0016568736007500384	0.0	0.3663307119707097	8.082310247561162E-5
86-87	2.694103415853721E-5	0.0017511672203049187	0.0	0.36643847610734387	8.082310247561162E-5
88	2.694103415853721E-5	0.0018858723910976046	0.0	0.36645194662442315	8.082310247561162E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5080	0.0	51.58908	1
GTATCAA	9300	0.0	36.38178	1
TCAACGC	10830	0.0	30.84434	4
ATCAACG	11000	0.0	30.483858	3
CAACGCA	11145	0.0	30.01079	5
TATCAAC	11370	0.0	29.645748	2
AACGCAG	11555	0.0	29.01968	6
ACGCAGA	13080	0.0	25.571121	7
CGCAGAG	13570	0.0	24.647772	8
GCAGAGT	15575	0.0	21.502174	9
GTACATG	11205	0.0	21.221138	1
TGGTATC	2250	0.0	21.02262	2
GAGTACT	9415	0.0	20.817347	12-13
TACATGG	11125	0.0	20.339529	2
ACATGGG	10980	0.0	20.139696	3
GTGGTAT	2485	0.0	19.891977	1
CAGAGTA	15335	0.0	19.393648	10-11
GTACTTT	10430	0.0	18.669193	14-15
TATACCG	255	4.157664E-8	18.379782	5
CATGGGG	7925	0.0	18.118341	4
>>END_MODULE