FastQCFastQC Report
Fri 27 May 2016
SRR2139464_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139464_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6680791
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81600.045.547731
GTATCAA145900.035.937441
TCAACGC165300.030.9282464
ATCAACG168950.030.4140663
CAACGCA168250.030.3859655
TATCAAC177600.029.3764132
AACGCAG174600.029.355376
ACGCAGA203000.025.2057887
CGCAGAG208950.024.446538
TGGTATC43950.021.0182612
GCAGAGT246900.020.7767689
GTGGTAT48150.020.626011
GTACATG186450.020.1433451
TACATGG188200.019.656472
GAGTACT141400.019.4732612-13
CTTATAC55450.019.2402021
ACATGGG186950.019.0892413
CAGAGTA242800.018.21653710-11
AGAGTAC221850.017.8942410-11
TATACAC75200.017.702513
Produced by FastQC (version 0.11.5)