##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139464_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6680791
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.574073339519227	31.0	18.0	33.0	18.0	33.0
2	31.442113815564653	33.0	32.0	33.0	27.0	34.0
3	32.45268427047037	33.0	33.0	33.0	31.0	34.0
4	32.584723575396985	33.0	33.0	34.0	31.0	34.0
5	33.16305344681491	33.0	33.0	34.0	33.0	34.0
6	36.995018703623565	38.0	37.0	38.0	35.0	38.0
7	37.11044410759145	38.0	38.0	38.0	36.0	38.0
8	37.05409464238591	38.0	38.0	38.0	36.0	38.0
9	37.315548712719796	38.0	38.0	38.0	36.0	38.0
10-11	37.38893792666168	38.0	38.0	38.0	37.0	38.0
12-13	37.510679573721134	38.0	38.0	38.0	37.0	38.0
14-15	37.487830782313054	38.0	38.0	38.0	37.0	38.0
16-17	37.50678130778227	38.0	38.0	38.0	37.0	38.0
18-19	37.52114308021311	38.0	38.0	38.0	37.0	38.0
20-21	37.52925491829617	38.0	38.0	38.0	37.5	38.0
22-23	37.54134199083073	38.0	38.0	38.0	38.0	38.0
24-25	37.54939601033804	38.0	38.0	38.0	38.0	38.0
26-27	37.52413300849073	38.0	38.0	38.0	38.0	38.0
28-29	37.501641083492416	38.0	38.0	38.0	38.0	38.0
30-31	37.49696226043082	38.0	38.0	38.0	38.0	38.0
32-33	37.40548805054087	38.0	38.0	38.0	37.0	38.0
34-35	37.45728683303997	38.0	38.0	38.0	37.0	38.0
36-37	37.44819894191657	38.0	38.0	38.0	37.0	38.0
38-39	37.42805001446548	38.0	38.0	38.0	37.0	38.0
40-41	37.423558448340216	38.0	38.0	38.0	37.0	38.0
42-43	37.389929808581776	38.0	38.0	38.0	37.0	38.0
44-45	37.35545122566678	38.0	38.0	38.0	37.0	38.0
46-47	37.330293273400876	38.0	38.0	38.0	37.0	38.0
48-49	37.213881981201276	38.0	38.0	38.0	37.0	38.0
50-51	37.20238943622303	38.0	38.0	38.0	37.0	38.0
52-53	37.20503525525933	38.0	38.0	38.0	37.0	38.0
54-55	37.18208306747256	38.0	38.0	38.0	36.5	38.0
56-57	37.11802357555039	38.0	38.0	38.0	36.0	38.0
58-59	37.092412015289824	38.0	38.0	38.0	36.0	38.0
60-61	37.09858755342124	38.0	38.0	38.0	36.0	38.0
62-63	37.09394396842853	38.0	38.0	38.0	36.0	38.0
64-65	37.09639794115895	38.0	38.0	38.0	36.0	38.0
66-67	37.02202014482914	38.0	38.0	38.0	36.0	38.0
68-69	37.03297758070451	38.0	38.0	38.0	36.0	38.0
70-71	37.04241460952845	38.0	38.0	38.0	36.0	38.0
72-73	37.0534539430639	38.0	38.0	38.0	36.0	38.0
74-75	37.03464796517528	38.0	38.0	38.0	36.0	38.0
76-77	36.99659800293106	38.0	38.0	38.0	36.0	38.0
78-79	36.99244768348094	38.0	38.0	38.0	36.0	38.0
80-81	36.98719459226211	38.0	38.0	38.0	36.0	38.0
82-83	36.91728888831788	38.0	38.0	38.0	35.0	38.0
84-85	36.9125170797426	38.0	38.0	38.0	35.0	38.0
86-87	36.9025763438506	38.0	38.0	38.0	35.0	38.0
88-89	36.87632985445779	38.0	38.0	38.0	35.0	38.0
90-91	36.84727972307652	38.0	38.0	38.0	35.0	38.0
92-93	36.82582481688048	38.0	38.0	38.0	35.0	38.0
94-95	36.81802887323859	38.0	38.0	38.0	35.0	38.0
96-97	36.789092673892135	38.0	38.0	38.0	35.0	38.0
98-99	36.809815600323354	38.0	38.0	38.0	35.0	38.0
100	35.33671865745774	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	19.0
21	91.0
22	315.0
23	1012.0
24	2466.0
25	5104.0
26	9095.0
27	15342.0
28	24297.0
29	36020.0
30	47134.0
31	60474.0
32	78698.0
33	105597.0
34	159295.0
35	293268.0
36	900385.0
37	4942174.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.808461153776555	19.25989003397951	14.401588075424002	25.530060736819937
2	14.338774601612963	19.788955058226808	43.688644264767106	22.183626075393125
3	18.82201057060617	25.47327486719385	29.620493983495965	26.084220578704016
4	12.79110512512665	17.801589662062472	38.2212375750117	31.186067637799177
5	14.100231544438376	36.889673692830684	35.217356747127695	13.792738015603243
6	30.705959818231104	37.17860654524292	18.489786613591118	13.625647022934858
7	27.435239330193085	31.94579803499316	22.470887055140626	18.148075579673126
8	24.19066844030894	37.43710886929407	20.687011463163568	17.68521122723342
9	25.224572958501472	17.847826701957896	21.454914545298603	35.47268579424203
10-11	24.83255351050497	26.95860415331059	28.149144315396185	20.059698020788257
12-13	25.369555790624194	24.014244720423076	28.709661775080225	21.906537713872503
14-15	22.42918540633886	25.50448741773242	26.355000478236786	25.711326697691934
16-17	22.156523082371535	28.669127652698613	27.08909019904978	22.085259065880074
18-19	22.15882071449324	27.7268140853381	28.74185107721526	21.372514122953405
20-21	22.801942968012217	26.902519448914614	28.699372686154323	21.596164896918843
22-23	22.560880592262784	26.862837671862504	28.61811837082528	21.958163365049437
24-25	22.37507831347396	27.011048842906725	28.71389252958696	21.89998031403235
26-27	22.291367687097924	27.096515040124242	28.70888061675173	21.903236656026106
28-29	22.16455307987064	27.042821921402243	28.77826476830213	22.014360230424987
30-31	22.43699332543048	26.915239425438287	28.71302617696547	21.93474107216576
32-33	22.210229951315412	27.127654427574505	28.67596753160785	21.98614808950223
34-35	22.410343488566244	27.15481269303573	28.709600865608405	21.72524295278962
36-37	22.40813415058806	27.024832125002863	28.54501507953052	22.022018644878564
38-39	22.25943529727657	27.109342589221676	28.730108286394717	21.90111382710704
40-41	22.541802993497686	26.95383866014206	28.57809960656944	21.926258739790814
42-43	22.466716507982095	27.037401044105142	28.45544570010359	22.04043674780917
44-45	22.41559071281237	27.217409052718455	28.37592294444215	21.991077290027025
46-47	22.587311142531785	27.11273700193836	28.060625146569006	22.239326708960853
48-49	22.488910346762395	27.198289625213956	28.02787669810861	22.28492332991504
50-51	22.411254608494705	27.359995886455224	28.039863874801323	22.188885630248745
52-53	22.587802992782652	27.304880863168325	27.99395147903766	22.11336466501137
54-55	22.567783661243848	27.24289495610791	27.889438778026015	22.29988260462223
56-57	22.59969547217723	27.415212708070147	27.815545874873116	22.16954594487951
58-59	22.48399532140435	27.48126282995445	27.990291986114794	22.044449862526406
60-61	22.622183078913025	27.586933536150227	27.8015138525191	21.98936953241765
62-63	22.507404127961195	27.731172200833647	27.774644936794424	21.986778734410734
64-65	22.472131195804977	27.694644102837547	27.77173055235475	22.061494149002726
66-67	22.539361273827858	27.703704522093176	27.534410582450143	22.222523621628827
68-69	22.500945836273996	27.617445912639667	27.875392185146325	22.006216065940013
70-71	22.706907498547064	27.37249265597905	27.82795085293761	22.092648992536276
72-73	22.567680905211123	27.108116146693906	27.96250851246822	22.36169443562675
74-75	22.445430617333855	27.28225468464896	28.076978255007486	22.1953364430097
76-77	22.575385731294958	27.376207842746602	27.706958493807644	22.341447932150793
78-79	22.57792451976142	27.279710028864802	27.665171778649793	22.47719367272398
80-81	22.484362756795548	27.283938409938575	27.846215574004272	22.385483259261605
82-83	22.599634452703935	27.24666044608346	27.848715369080885	22.304989732131723
84-85	22.63531718717958	27.29191465847614	27.709638355935134	22.363129798409144
86-87	22.505644492125924	27.301835101977034	27.786934076817175	22.40558632907986
88-89	22.577101664613764	27.326439624117	27.719526454493426	22.37693225677581
90-91	22.55990726442803	27.32777382742328	27.708703505963722	22.403615402184965
92-93	22.615062304630154	27.295809754871097	27.741841433647657	22.34728650685109
94-95	22.464989414866825	27.40293469679638	27.71713866779253	22.414937220544275
96-97	22.616058145063576	27.469430129296462	27.76650140339576	22.148010322244204
98-99	23.706984612708755	29.428835864021657	27.93701004829538	18.92716947497421
100	30.25883391915332	40.51695828656742	29.224207794279256	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1116.0
1	1044.5
2	1127.5
3	1415.0
4	1880.0
5	2800.5
6	3605.5
7	4438.0
8	5490.0
9	6114.0
10	6260.0
11	6357.0
12	7115.0
13	8968.5
14	12500.0
15	16729.0
16	20304.5
17	22571.0
18	22763.0
19	21278.5
20	19208.0
21	17583.5
22	17455.0
23	19859.0
24	24685.0
25	31757.0
26	42051.5
27	54495.0
28	67530.0
29	82324.0
30	96724.5
31	111006.0
32	127772.5
33	145241.5
34	161672.5
35	178343.5
36	196722.5
37	212228.0
38	223916.0
39	234504.5
40	246089.5
41	256471.0
42	263450.5
43	272871.5
44	282992.0
45	289359.5
46	290561.5
47	288307.0
48	285298.5
49	281839.0
50	276976.0
51	268914.0
52	257467.5
53	242017.5
54	224295.0
55	206918.0
56	188581.0
57	169287.0
58	150087.5
59	127710.5
60	104638.5
61	83626.5
62	65258.0
63	49719.0
64	36391.0
65	26658.0
66	19767.5
67	14990.5
68	11367.0
69	8351.0
70	6057.5
71	4242.5
72	2856.0
73	1826.5
74	1140.0
75	743.0
76	463.5
77	264.5
78	149.5
79	96.0
80	68.5
81	45.5
82	33.0
83	22.0
84	12.0
85	6.0
86	4.5
87	3.5
88	2.5
89	3.0
90	2.0
91	0.5
92	1.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.011854883650753332
3	1.4968287437819861E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	7.48456663648141E-6
24-25	3.817416415983418E-4
26-27	3.518311689478302E-4
28-29	0.0031596480301840724
30-31	0.013326983726022383
32-33	0.008490296948135762
34-35	9.01479485594771E-4
36-37	1.3555292716756285E-4
38-39	9.297932752058831E-4
40-41	0.0
42-43	0.001792180844622039
44-45	7.053960806660288E-4
46-47	0.0014275684928071381
48-49	0.003681463899168898
50-51	0.002438059801387209
52-53	0.0
54-55	0.0018016736600052156
56-57	0.04971958488082526
58-59	0.06994735419070215
60-61	0.04760628490015859
62-63	0.014921734366662083
64-65	0.0
66-67	0.0
68-69	1.9964841913390518E-4
70-71	0.001948122996088891
72-73	0.005841382472953085
74-75	0.0105491472288228
76-77	0.019427793282070865
78-79	0.014412913970917943
80-81	0.006617896373761913
82-83	0.038494675391298194
84-85	0.02792585336634161
86-87	0.00838193787329349
88-89	0.0028312994860917435
90-91	0.0033511751869757266
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	49.0
20-21	219.0
22-23	510.0
24-25	488.0
26-27	1106.0
28-29	2242.0
30-31	7540.0
32-33	9798.0
34-35	14078.0
36-37	23402.0
38-39	28226.0
40-41	29142.0
42-43	33027.0
44-45	40595.0
46-47	42461.0
48-49	40172.0
50-51	36065.0
52-53	34664.0
54-55	38971.0
56-57	40574.0
58-59	43062.0
60-61	44550.0
62-63	44412.0
64-65	48250.0
66-67	52807.0
68-69	54771.0
70-71	59562.0
72-73	49253.0
74-75	49442.0
76-77	52689.0
78-79	55446.0
80-81	55496.0
82-83	52174.0
84-85	52392.0
86-87	54428.0
88-89	57747.0
90-91	59139.0
92-93	57954.0
94-95	67634.0
96-97	214099.0
98-99	1621551.0
100-101	3410604.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.26324653687809
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.07099395990812	76.79745363825303
2	6.945749436244175	11.844342931317382
3	1.6655321322079801	4.260260304106237
4	0.5849134013649157	1.9948646217320292
5	0.27736005989504153	1.1824309583157102
6	0.14405507574691065	0.7369562062978494
7	0.08695973489175417	0.5190128520400037
8	0.0569354183694752	0.3883598890493489
9	0.03628504007591341	0.27844022858337963
>10	0.12765090947136304	1.7019173222446689
>50	0.002884823824384774	0.15979741144727738
>100	6.22430901066183E-4	0.09428807240108615
>500	2.8788550407713692E-5	0.016151302682207706
>1k	2.8788548495440015E-5	0.025724261529802398
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	5.987314975127945E-5	0.0	0.0	0.0
5	0.0	5.987314975127945E-5	0.0	1.4968287437819863E-5	0.0
6	5.987314975127945E-5	5.987314975127945E-5	0.0	1.4968287437819863E-5	0.0
7	5.987314975127945E-5	7.484143718909932E-5	0.0	1.4968287437819863E-5	1.4968287437819863E-5
8	5.987314975127945E-5	8.980972462691917E-5	0.0	1.4968287437819863E-5	4.4904862313459586E-5
9	5.987314975127945E-5	1.7961944925383834E-4	0.0	2.9936574875639726E-5	5.987314975127945E-5
10-11	7.484143718909932E-5	1.8710359297274828E-4	0.0	2.9936574875639726E-5	5.987314975127945E-5
12-13	7.484143718909932E-5	1.9458773669165822E-4	0.0	2.9936574875639726E-5	5.987314975127945E-5
14-15	8.980972462691917E-5	2.0955602412947808E-4	0.0	4.4904862313459586E-5	8.232558090800925E-5
16-17	9.729386834582911E-5	2.0955602412947808E-4	0.0	5.987314975127945E-5	8.980972462691917E-5
18-19	1.0477801206473904E-4	2.394925990051178E-4	0.0	5.987314975127945E-5	1.1226215578364896E-4
20-21	1.0477801206473904E-4	2.694291738807575E-4	0.0	7.484143718909932E-5	1.3471458694037876E-4
22-23	1.0477801206473904E-4	3.068498924753072E-4	0.0	8.980972462691917E-5	1.7961944925383834E-4
24-25	1.0477801206473904E-4	3.3678646735094695E-4	0.0	1.197462995025589E-4	2.2452431156729796E-4
26-27	1.0477801206473904E-4	3.4427061106985686E-4	0.0	1.2723044322146884E-4	2.2452431156729796E-4
28-29	1.0477801206473904E-4	3.4427061106985686E-4	0.0	2.394925990051178E-4	2.394925990051178E-4
30-31	1.0477801206473904E-4	4.041437608211363E-4	0.0	8.082875216422727E-4	2.619450301618476E-4
32-33	1.197462995025589E-4	4.265961919778661E-4	0.0	0.0027092600262453954	2.843974613185774E-4
34-35	1.197462995025589E-4	5.38858347761515E-4	0.0	0.004565327668535058	2.843974613185774E-4
36-37	1.197462995025589E-4	6.436363598262541E-4	0.0	0.007820930186260878	2.9188160503748736E-4
38-39	1.197462995025589E-4	6.885412221397137E-4	0.0	0.01627052844491019	3.143340361942171E-4
40-41	1.3471458694037876E-4	7.783509467666329E-4	0.0	0.025251500907602108	3.143340361942171E-4
42-43	1.3471458694037876E-4	8.008033779233626E-4	0.0	0.030842156265627828	3.143340361942171E-4
44-45	1.3471458694037876E-4	9.205496774259217E-4	0.0	0.03815416467900283	3.143340361942171E-4
46-47	1.3471458694037876E-4	0.0010178435457717508	0.0	0.04610232530848518	3.517547547887668E-4
48-49	1.421987306592887E-4	0.0010328118332095706	0.0	0.05367627875202203	3.8169132966440653E-4
50-51	1.7213530553492843E-4	0.0011226215578364898	0.0	0.06142985164481271	3.8917547338331645E-4
52-53	1.9458773669165822E-4	0.0011525581327121294	0.0	0.08222080289594451	4.0414376082113627E-4
54-55	1.9458773669165822E-4	0.0011525581327121294	0.0	0.10777915369602192	4.1911204825895615E-4
56-57	1.9458773669165822E-4	0.0011525581327121294	0.0	0.140013360693367	4.4156447941568595E-4
58-59	1.9458773669165822E-4	0.0011600422764310393	0.0	0.16649226117087035	4.715010542913257E-4
60-61	2.0955602412947808E-4	0.0012199154261823188	0.0	0.17511399473505457	4.789851980102356E-4
62-63	2.0955602412947808E-4	0.0012573361447768684	0.0	0.1862204640139169	5.014376291669655E-4
64-65	2.0955602412947808E-4	0.0013770824442794274	0.0	0.19188596080913173	5.164059166047853E-4
66-67	2.0955602412947808E-4	0.0013770824442794274	0.0	0.19701259925658504	5.238900603236952E-4
68-69	2.0955602412947808E-4	0.001414503162873977	0.0	0.20244608759651364	5.238900603236952E-4
70-71	2.0955602412947808E-4	0.0014294714503117968	0.0	0.2069365738278596	5.463424914804249E-4
72-73	2.394925990051178E-4	0.0014444397377496167	0.0	0.21080587613053603	6.436363598262541E-4
74-75	2.394925990051178E-4	0.0015043128875008964	0.0	0.21406147864826186	6.436363598262541E-4
76-77	2.394925990051178E-4	0.0015941226121278153	0.0	0.2172796604473931	6.436363598262541E-4
78-79	2.394925990051178E-4	0.0016315433307223651	0.0	0.21929289510777988	6.436363598262541E-4
80-81	2.394925990051178E-4	0.001639027474441275	0.0	0.22008621434198436	6.58604647264074E-4
82-83	2.394925990051178E-4	0.0016614799055980048	0.0	0.22060262025858915	7.035095095775335E-4
84-85	2.394925990051178E-4	0.0016614799055980048	0.0	0.22080469213899973	7.035095095775335E-4
86-87	2.394925990051178E-4	0.0016914164804736444	0.0	0.22089450186362664	7.259619407342633E-4
88	2.394925990051178E-4	0.0017812262051005636	0.0	0.22090198600734554	7.334460844531733E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8160	0.0	45.54773	1
GTATCAA	14590	0.0	35.93744	1
TCAACGC	16530	0.0	30.928246	4
ATCAACG	16895	0.0	30.414066	3
CAACGCA	16825	0.0	30.385965	5
TATCAAC	17760	0.0	29.376413	2
AACGCAG	17460	0.0	29.35537	6
ACGCAGA	20300	0.0	25.205788	7
CGCAGAG	20895	0.0	24.44653	8
TGGTATC	4395	0.0	21.018261	2
GCAGAGT	24690	0.0	20.776768	9
GTGGTAT	4815	0.0	20.62601	1
GTACATG	18645	0.0	20.143345	1
TACATGG	18820	0.0	19.65647	2
GAGTACT	14140	0.0	19.47326	12-13
CTTATAC	5545	0.0	19.240202	1
ACATGGG	18695	0.0	19.089241	3
CAGAGTA	24280	0.0	18.216537	10-11
AGAGTAC	22185	0.0	17.89424	10-11
TATACAC	7520	0.0	17.70251	3
>>END_MODULE