FastQCFastQC Report
Fri 27 May 2016
SRR2139463_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139463_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5710277
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72200.050.669551
GTATCAA137400.039.762431
TCAACGC161750.032.90544
ATCAACG164500.032.648963
CAACGCA167700.031.7379135
TATCAAC171450.031.5326022
AACGCAG173400.030.7959276
ACGCAGA199250.026.7785327
CGCAGAG205850.025.898628
GTGGTAT37700.024.8128991
TGGTATC35950.023.8218212
GCAGAGT238850.022.33889
GTACATG183550.021.4144971
TACATGG184500.020.566322
GAGTACT134450.020.23435212-13
ACATGGG181350.020.1956273
AGAGTAC215650.019.1623610-11
CAGAGTA234400.019.1470610-11
GTACTTT146300.018.40070514-15
CATGGGG130600.018.0231384
Produced by FastQC (version 0.11.5)