FastQCFastQC Report
Fri 27 May 2016
SRR2139462_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139462_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3824948
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48750.053.0375751
GTATCAA89050.038.466661
TCAACGC107400.031.0301174
ATCAACG108550.030.9443973
TATCAAC112350.030.215182
CAACGCA111250.029.9562665
AACGCAG114750.029.0808856
ACGCAGA130700.025.5319927
CGCAGAG136600.024.429228
TGGTATC23000.023.5157182
GTGGTAT26250.021.6093311
GCAGAGT157400.021.3126939
GTACATG113150.021.1021271
GAGTACT91850.020.91803212-13
TACATGG115650.019.8094772
GTACTTT98700.019.3999614-15
ACATGGG115200.019.3115233
CAGAGTA156400.018.8908310-11
AGAGTAC143450.018.69596110-11
AGTACTT102000.017.26318212-13
Produced by FastQC (version 0.11.5)