##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139462_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3824948
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.504300450620505	31.0	18.0	33.0	18.0	33.0
2	31.439581923728113	33.0	32.0	33.0	27.0	33.0
3	32.46975514438366	33.0	33.0	33.0	31.0	34.0
4	32.616206024238764	33.0	33.0	34.0	31.0	34.0
5	33.178479550571666	33.0	33.0	34.0	33.0	34.0
6	37.03463158191955	38.0	37.0	38.0	35.0	38.0
7	37.14081028029662	38.0	38.0	38.0	36.0	38.0
8	37.08664091642553	38.0	38.0	38.0	36.0	38.0
9	37.32982017010427	38.0	38.0	38.0	37.0	38.0
10-11	37.38438548707067	38.0	38.0	38.0	37.0	38.0
12-13	37.51334501802377	38.0	38.0	38.0	37.0	38.0
14-15	37.49459195262263	38.0	38.0	38.0	37.0	38.0
16-17	37.51648584503633	38.0	38.0	38.0	37.0	38.0
18-19	37.52980916341869	38.0	38.0	38.0	37.0	38.0
20-21	37.53527851912115	38.0	38.0	38.0	38.0	38.0
22-23	37.54588175806623	38.0	38.0	38.0	38.0	38.0
24-25	37.55441257396625	38.0	38.0	38.0	38.0	38.0
26-27	37.53102265305095	38.0	38.0	38.0	38.0	38.0
28-29	37.51077852031217	38.0	38.0	38.0	38.0	38.0
30-31	37.50553823556534	38.0	38.0	38.0	38.0	38.0
32-33	37.41840598961741	38.0	38.0	38.0	37.0	38.0
34-35	37.46688876229519	38.0	38.0	38.0	37.0	38.0
36-37	37.45693076786942	38.0	38.0	38.0	37.0	38.0
38-39	37.44412017605386	38.0	38.0	38.0	37.0	38.0
40-41	37.426580236580136	38.0	38.0	38.0	37.0	38.0
42-43	37.39261000466381	38.0	38.0	38.0	37.0	38.0
44-45	37.35517642974737	38.0	38.0	38.0	37.0	38.0
46-47	37.32132323129366	38.0	38.0	38.0	37.0	38.0
48-49	37.210153776104406	38.0	38.0	38.0	37.0	38.0
50-51	37.19607313204406	38.0	38.0	38.0	37.0	38.0
52-53	37.19732522024427	38.0	38.0	38.0	37.0	38.0
54-55	37.176527698678974	38.0	38.0	38.0	36.5	38.0
56-57	37.12086485254934	38.0	38.0	38.0	36.0	38.0
58-59	37.098946162406264	38.0	38.0	38.0	36.0	38.0
60-61	37.109245369588606	38.0	38.0	38.0	36.0	38.0
62-63	37.10630995716889	38.0	38.0	38.0	36.0	38.0
64-65	37.11072112026075	38.0	38.0	38.0	36.0	38.0
66-67	37.04438976794664	38.0	38.0	38.0	36.0	38.0
68-69	37.05810733452114	38.0	38.0	38.0	36.0	38.0
70-71	37.067211932376765	38.0	38.0	38.0	36.0	38.0
72-73	37.07776451636407	38.0	38.0	38.0	36.0	38.0
74-75	37.06303433348968	38.0	38.0	38.0	36.0	38.0
76-77	37.02331783931212	38.0	38.0	38.0	36.0	38.0
78-79	37.01774913679726	38.0	38.0	38.0	36.0	38.0
80-81	37.01286326914638	38.0	38.0	38.0	36.0	38.0
82-83	36.948285448779046	38.0	38.0	38.0	35.5	38.0
84-85	36.94267189949651	38.0	38.0	38.0	35.5	38.0
86-87	36.93347149224392	38.0	38.0	38.0	35.0	38.0
88-89	36.904505915357866	38.0	38.0	38.0	35.0	38.0
90-91	36.87448742072012	38.0	38.0	38.0	35.0	38.0
92-93	36.85401259320513	38.0	38.0	38.0	35.0	38.0
94-95	36.84897857973308	38.0	38.0	38.0	35.0	38.0
96-97	36.8183185604786	38.0	38.0	38.0	35.0	38.0
98-99	36.84019052646697	38.0	38.0	38.0	35.0	38.0
100	35.301379999491715	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	13.0
21	46.0
22	179.0
23	505.0
24	1257.0
25	2552.0
26	4862.0
27	8368.0
28	13677.0
29	20061.0
30	27374.0
31	35502.0
32	45732.0
33	60629.0
34	90294.0
35	166015.0
36	504789.0
37	2843088.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.170180614220115	19.21633967311451	14.609741099748282	25.003738612917093
2	14.421820111934883	20.0246042339677	43.16801083527583	22.385564818821585
3	18.61777917910475	25.57947223307205	29.8396630958973	25.963085491925895
4	12.757637489450838	17.95783890395373	38.46794779955178	30.81657580704365
5	14.027380241509166	36.946567639612354	35.26630950276971	13.759742616108769
6	30.495264249344046	37.27546099973124	18.67583559305904	13.553439157865677
7	27.324842063212362	32.16956152083636	22.56906499121034	17.936531424740938
8	24.45348276630166	36.941547963527874	20.725275219427818	17.87969405074265
9	25.331769216208954	17.83054305574873	21.468082703346557	35.36960502469576
10-11	24.6075240761443	26.919960741949957	28.31488166636514	20.157633515540603
12-13	25.301154420922845	24.135217001642896	28.83355538428235	21.73007319315191
14-15	22.418566213187734	25.581994840191292	26.607198320081736	25.392240626539238
16-17	21.954037022202655	28.874013450640373	27.241050074406242	21.93089945275073
18-19	21.92078166814294	27.864888620708044	29.086238558014383	21.128091153134633
20-21	22.62358150071701	26.96230363891391	29.15282863234512	21.261286228023966
22-23	22.4436232452816	26.81315345620469	29.005370011548443	21.73785328696527
24-25	22.219742605305168	26.921580001440702	29.07434941759289	21.78432797566123
26-27	22.107225151480602	27.115367920314874	29.030657498512124	21.746749429692393
28-29	22.091389405026415	27.035538667771053	29.10379244153225	21.769279485670282
30-31	22.314893220610415	26.973296517305663	29.000315057552957	21.711495204530966
32-33	22.103519375916285	27.169249445565207	28.924137867814366	21.803093310704146
34-35	22.27222532392386	27.12227851905387	28.933128857040284	21.672367299981985
36-37	22.23028466556122	27.03110837487287	28.895634889822837	21.84297206974307
38-39	22.08597632924754	27.190854650941343	28.993871109443457	21.729297910367663
40-41	22.368283049544807	27.046154712966906	28.813904991936756	21.771657245551527
42-43	22.189784607541224	27.154624926895877	28.710811709670587	21.944778755892308
44-45	22.241434870013187	27.26080431340245	28.59928593683977	21.898474879744594
46-47	22.366347645812812	27.21352238511279	28.360905620046484	22.059224349027915
48-49	22.276313016749963	27.331062914017178	28.29312207029432	22.09950199893854
50-51	22.27452754661944	27.472175895617923	28.188510197436102	22.064786360326544
52-53	22.452756186654153	27.39255361076451	28.142264835732117	22.012425366849225
54-55	22.441710993363152	27.329283150513533	28.095379956320482	22.133625899802833
56-57	22.321739491121097	27.455480089633067	28.174650257548308	22.048130161697532
58-59	22.39555275961851	27.390879669381984	28.140889165278786	22.072678405720715
60-61	22.40422262627164	27.432932134570763	28.18078763608781	21.982057603069784
62-63	22.387134253481054	27.471349321429265	28.195947070543397	21.945569354546286
64-65	22.40018179476629	27.37914096894861	28.15403974969084	22.06663748659426
66-67	22.431314735344525	27.441530958016003	27.99016391254419	22.136990394095278
68-69	22.325302031850633	27.434345254438952	28.269194467325647	21.97115824638477
70-71	22.520847473817383	27.215255254857208	28.235531250211608	22.028366021113797
72-73	22.39582320175712	27.117643386905478	28.232496628453386	22.25403678288401
74-75	22.300277730007593	27.34380026837095	28.29435871158546	22.061563290035995
76-77	22.399390209311274	27.40073771942349	28.05471848826605	22.145153582999182
78-79	22.384743147983823	27.271352644332676	28.00871249738949	22.335191710294016
80-81	22.376404469136503	27.346543818837898	28.0670789887972	22.209972723228397
82-83	22.49393637011442	27.33802568331805	28.016946734947602	22.151091211619928
84-85	22.46462997849873	27.348054475567494	27.982111163555707	22.205204382378067
86-87	22.372307293118045	27.37026912291126	28.058518131270443	22.19890545270025
88-89	22.451841385440368	27.373307418897745	27.947616040744457	22.227235154917434
90-91	22.44751482876395	27.44554422630677	27.9266495021278	22.180291442801487
92-93	22.462570747312906	27.438885322707495	27.903770869855567	22.194773060124028
94-95	22.371187452028387	27.47893711479272	27.917439099956905	22.232436333221983
96-97	22.516339795311588	27.54857315989152	27.9397969930185	21.995290051778387
98-99	23.559193570191926	29.45457399904931	28.17232792718931	18.81390450356945
100	22.476234815383126	30.349012762794565	22.12293844458989	25.051813977232424
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	730.0
1	611.0
2	650.0
3	869.0
4	1089.5
5	1530.0
6	2029.0
7	2829.0
8	3852.0
9	4515.0
10	4590.0
11	4492.5
12	4875.5
13	5932.0
14	8091.0
15	10731.5
16	12660.0
17	13740.5
18	13822.5
19	12863.0
20	11567.5
21	10553.0
22	10371.0
23	11631.5
24	14556.0
25	19156.5
26	25091.5
27	31894.5
28	39425.5
29	48099.0
30	56346.0
31	64727.0
32	74654.0
33	84179.0
34	93112.5
35	102095.0
36	111839.5
37	120367.0
38	127149.5
39	133245.5
40	138942.5
41	144223.5
42	146925.0
43	150060.0
44	153410.5
45	155564.5
46	156264.0
47	155110.0
48	154223.0
49	152807.0
50	151206.5
51	147678.0
52	142257.5
53	134796.5
54	125006.0
55	116427.5
56	106962.5
57	95826.0
58	84950.5
59	73680.0
60	61524.5
61	49678.0
62	39307.5
63	30231.0
64	22563.0
65	16532.0
66	12206.5
67	9244.0
68	7063.0
69	5317.5
70	3890.5
71	2787.5
72	1961.0
73	1254.5
74	770.5
75	463.0
76	271.0
77	158.0
78	94.0
79	62.5
80	44.0
81	27.5
82	15.0
83	10.0
84	5.5
85	3.5
86	3.0
87	1.5
88	1.0
89	1.0
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.010536090948164524
3	5.22882925467222E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	4.4449877207214215E-4
26-27	4.5760373320970183E-4
28-29	0.0025760806854623793
30-31	0.012323407016492696
32-33	0.0074115579186407364
34-35	7.080396061621474E-4
36-37	9.196694970590283E-5
38-39	7.512292482804758E-4
40-41	1.3226019177198766E-5
42-43	0.0014471253584954359
44-45	6.402198941929921E-4
46-47	0.0013144816985249637
48-49	0.002711586365712058
50-51	0.0018851261108681994
52-53	0.0
54-55	0.0015614258776754314
56-57	0.045771223770733414
58-59	0.06461349795921646
60-61	0.04327559305332654
62-63	0.01281441046351293
64-65	0.0
66-67	0.0
68-69	2.0021010620574107E-4
70-71	0.0012966694756369492
72-73	0.005109682529037368
74-75	0.009124247688231476
76-77	0.01719271665253829
78-79	0.012371475150637188
80-81	0.005077625510589694
82-83	0.035239131416584385
84-85	0.024743866718722685
86-87	0.007246302274893697
88-89	0.0023541127666477975
90-91	0.00320476443730746
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	25.0
20-21	110.0
22-23	237.0
24-25	222.0
26-27	487.0
28-29	1065.0
30-31	3423.0
32-33	4568.0
34-35	6634.0
36-37	11113.0
38-39	13376.0
40-41	13768.0
42-43	16403.0
44-45	20205.0
46-47	21854.0
48-49	20151.0
50-51	18173.0
52-53	17722.0
54-55	20360.0
56-57	20644.0
58-59	21478.0
60-61	21408.0
62-63	21285.0
64-65	22975.0
66-67	24593.0
68-69	25641.0
70-71	26225.0
72-73	25205.0
74-75	25168.0
76-77	26782.0
78-79	28417.0
80-81	28218.0
82-83	26849.0
84-85	26483.0
86-87	27940.0
88-89	29744.0
90-91	30634.0
92-93	30071.0
94-95	35664.0
96-97	121869.0
98-99	272758.0
100-101	2715001.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.93480417015415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.6486661651279	83.32339648186763
2	5.576033214539409	10.029589103917539
3	1.0843654265105789	2.9256657684634377
4	0.33343655092978464	1.1995020364416722
5	0.1421335632976769	0.6391377090591391
6	0.07452771237939362	0.4021581130854203
7	0.04064599488587914	0.2558842713253839
8	0.027605572753274132	0.1986161423656522
9	0.018474852177343883	0.14953789913777712
>10	0.052762151014847956	0.764934469587533
>50	0.0010326916538024187	0.06435839907157664
>100	3.1610473016461594E-4	0.047219605677254944
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	2.6144146273361102E-5
3	0.0	0.0	0.0	2.6144146273361102E-5	2.6144146273361102E-5
4	0.0	0.0	0.0	2.6144146273361102E-5	5.2288292546722203E-5
5	0.0	2.6144146273361102E-5	0.0	2.6144146273361102E-5	5.2288292546722203E-5
6	0.0	2.6144146273361102E-5	0.0	2.6144146273361102E-5	5.2288292546722203E-5
7	0.0	2.6144146273361102E-5	0.0	2.6144146273361102E-5	5.2288292546722203E-5
8	0.0	5.2288292546722203E-5	0.0	2.6144146273361102E-5	7.843243882008331E-5
9	0.0	5.2288292546722203E-5	0.0	5.2288292546722203E-5	7.843243882008331E-5
10-11	0.0	6.536036568340276E-5	0.0	1.0457658509344441E-4	7.843243882008331E-5
12-13	0.0	7.843243882008331E-5	0.0	1.3072073136680552E-4	7.843243882008331E-5
14-15	2.6144146273361102E-5	7.843243882008331E-5	0.0	1.5686487764016662E-4	7.843243882008331E-5
16-17	2.6144146273361102E-5	7.843243882008331E-5	0.0	1.8300902391352772E-4	7.843243882008331E-5
18-19	2.6144146273361102E-5	9.150451195676386E-5	0.0	2.0915317018688881E-4	7.843243882008331E-5
20-21	2.6144146273361102E-5	1.0457658509344441E-4	0.0	2.0915317018688881E-4	7.843243882008331E-5
22-23	2.6144146273361102E-5	1.0457658509344441E-4	0.0	2.483693895969305E-4	7.843243882008331E-5
24-25	2.6144146273361102E-5	1.0457658509344441E-4	0.0	2.745135358702916E-4	7.843243882008331E-5
26-27	2.6144146273361102E-5	1.0457658509344441E-4	0.0	3.1372975528033323E-4	7.843243882008331E-5
28-29	2.6144146273361102E-5	1.0457658509344441E-4	0.0	4.5752255978381926E-4	7.843243882008331E-5
30-31	2.6144146273361102E-5	1.0457658509344441E-4	0.0	9.281171927043191E-4	7.843243882008331E-5
32-33	2.6144146273361102E-5	1.0457658509344441E-4	0.0	0.0015686487764016662	7.843243882008331E-5
34-35	2.6144146273361102E-5	1.4379280450348608E-4	0.0	0.0027320632855662354	1.0457658509344441E-4
36-37	5.2288292546722203E-5	1.5686487764016662E-4	0.0	0.004392216573924666	1.0457658509344441E-4
38-39	5.2288292546722203E-5	1.8300902391352772E-4	0.0	0.007019703274397456	1.1764865823012496E-4
40-41	6.536036568340276E-5	2.0915317018688881E-4	0.0	0.009790982779373732	1.3072073136680552E-4
42-43	7.843243882008331E-5	2.0915317018688881E-4	0.0	0.01256226228435001	1.3072073136680552E-4
44-45	7.843243882008331E-5	2.352973164602499E-4	0.0	0.01617015447007384	1.3072073136680552E-4
46-47	7.843243882008331E-5	2.6144146273361104E-4	0.0	0.019738830436387633	1.3072073136680552E-4
48-49	7.843243882008331E-5	2.745135358702916E-4	0.0	0.023176785671334618	1.5686487764016662E-4
50-51	7.843243882008331E-5	3.137297552803332E-4	0.0	0.02705919139292874	1.5686487764016662E-4
52-53	7.843243882008331E-5	3.398739015536943E-4	0.0	0.031242254796666515	1.5686487764016662E-4
54-55	7.843243882008331E-5	3.398739015536943E-4	0.0	0.035229237103354086	1.5686487764016662E-4
56-57	7.843243882008331E-5	3.398739015536943E-4	0.0	0.039882895140012364	1.5686487764016662E-4
58-59	7.843243882008331E-5	3.398739015536943E-4	0.0	0.044484264884123915	1.5686487764016662E-4
60-61	9.150451195676386E-5	3.5294597469037487E-4	0.0	0.048994130116278706	1.5686487764016662E-4
62-63	1.0457658509344441E-4	3.6601804782705543E-4	0.0	0.05341249083647673	1.5686487764016662E-4
64-65	1.1764865823012496E-4	3.6601804782705543E-4	0.0	0.05760862631335119	1.5686487764016662E-4
66-67	1.3072073136680552E-4	3.6601804782705543E-4	0.0	0.06223614020373611	1.5686487764016662E-4
68-69	1.3072073136680552E-4	3.79090120963736E-4	0.0	0.06686365409412101	1.5686487764016662E-4
70-71	1.3072073136680552E-4	3.921621941004165E-4	0.0	0.07119051030236229	1.8300902391352772E-4
72-73	1.5686487764016662E-4	3.921621941004165E-4	0.0	0.07468075382985599	2.352973164602499E-4
74-75	1.6993695077684715E-4	4.0523426723709707E-4	0.0	0.07725595223778206	2.352973164602499E-4
76-77	1.8300902391352772E-4	4.1830634037377763E-4	0.0	0.07973964613375137	2.352973164602499E-4
78-79	1.8300902391352772E-4	4.1830634037377763E-4	0.0	0.08136058320269976	2.352973164602499E-4
80-81	1.8300902391352772E-4	4.1830634037377763E-4	0.0	0.08192268234757702	2.352973164602499E-4
82-83	1.8300902391352772E-4	4.1830634037377763E-4	0.0	0.08227562832226738	2.352973164602499E-4
84-85	1.8300902391352772E-4	4.1830634037377763E-4	0.0	0.08235406076108748	2.352973164602499E-4
86-87	1.8300902391352772E-4	4.1830634037377763E-4	0.0	0.08239327698049752	2.352973164602499E-4
88	1.8300902391352772E-4	4.705946329204998E-4	0.0	0.0824063490536342	2.352973164602499E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4875	0.0	53.037575	1
GTATCAA	8905	0.0	38.46666	1
TCAACGC	10740	0.0	31.030117	4
ATCAACG	10855	0.0	30.944397	3
TATCAAC	11235	0.0	30.21518	2
CAACGCA	11125	0.0	29.956266	5
AACGCAG	11475	0.0	29.080885	6
ACGCAGA	13070	0.0	25.531992	7
CGCAGAG	13660	0.0	24.42922	8
TGGTATC	2300	0.0	23.515718	2
GTGGTAT	2625	0.0	21.609331	1
GCAGAGT	15740	0.0	21.312693	9
GTACATG	11315	0.0	21.102127	1
GAGTACT	9185	0.0	20.918032	12-13
TACATGG	11565	0.0	19.809477	2
GTACTTT	9870	0.0	19.39996	14-15
ACATGGG	11520	0.0	19.311523	3
CAGAGTA	15640	0.0	18.89083	10-11
AGAGTAC	14345	0.0	18.695961	10-11
AGTACTT	10200	0.0	17.263182	12-13
>>END_MODULE