FastQCFastQC Report
Fri 27 May 2016
SRR2139461_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139461_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4735457
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67450.049.1529241
GTATCAA122650.038.899281
TCAACGC144400.032.463274
ATCAACG145750.032.2818153
CAACGCA147300.031.7651565
TATCAAC151450.031.5021272
AACGCAG152700.030.6418296
ACGCAGA174900.026.9263527
CGCAGAG177700.026.4531758
TGGTATC31300.023.3223192
GCAGAGT204700.022.9639959
GTGGTAT36000.022.5707451
GAGTACT124400.020.37785512-13
CAGAGTA204200.019.52038410-11
GTACATG144350.019.2048381
TACATGG144100.018.8461592
AGAGTAC189300.018.84215410-11
ACATGGG140800.018.7294063
GTACTTT135400.018.51397714-15
CATGGGG99550.017.1073534
Produced by FastQC (version 0.11.5)