##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139461_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4735457
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.775090133856143	31.0	18.0	33.0	18.0	33.0
2	31.519346073673564	33.0	32.0	33.0	27.0	33.0
3	32.508279559924205	33.0	33.0	33.0	31.0	34.0
4	32.663003803012046	33.0	33.0	34.0	31.0	34.0
5	33.193020652494575	33.0	33.0	34.0	33.0	34.0
6	37.07577895860948	38.0	38.0	38.0	36.0	38.0
7	37.19157432957368	38.0	38.0	38.0	36.0	38.0
8	37.13571150577442	38.0	38.0	38.0	36.0	38.0
9	37.35347823874232	38.0	38.0	38.0	37.0	38.0
10-11	37.413791213815266	38.0	38.0	38.0	37.0	38.0
12-13	37.53099721948695	38.0	38.0	38.0	37.5	38.0
14-15	37.51232183081802	38.0	38.0	38.0	37.0	38.0
16-17	37.530052854455235	38.0	38.0	38.0	37.5	38.0
18-19	37.54030139012983	38.0	38.0	38.0	38.0	38.0
20-21	37.546155746099956	38.0	38.0	38.0	38.0	38.0
22-23	37.554147292962284	38.0	38.0	38.0	38.0	38.0
24-25	37.56385328266116	38.0	38.0	38.0	38.0	38.0
26-27	37.53605437497326	38.0	38.0	38.0	38.0	38.0
28-29	37.51329961207969	38.0	38.0	38.0	38.0	38.0
30-31	37.508666949165054	38.0	38.0	38.0	38.0	38.0
32-33	37.42277834058095	38.0	38.0	38.0	37.0	38.0
34-35	37.46653800187974	38.0	38.0	38.0	37.0	38.0
36-37	37.45117728016301	38.0	38.0	38.0	37.0	38.0
38-39	37.438046969824256	38.0	38.0	38.0	37.0	38.0
40-41	37.423554147153894	38.0	38.0	38.0	37.0	38.0
42-43	37.387076400004815	38.0	38.0	38.0	37.0	38.0
44-45	37.34736085894207	38.0	38.0	38.0	37.0	38.0
46-47	37.314131171609745	38.0	38.0	38.0	37.0	38.0
48-49	37.198916970613	38.0	38.0	38.0	37.0	38.0
50-51	37.18761767539038	38.0	38.0	38.0	37.0	38.0
52-53	37.193267421348956	38.0	38.0	38.0	37.0	38.0
54-55	37.174554443798456	38.0	38.0	38.0	37.0	38.0
56-57	37.11780567102706	38.0	38.0	38.0	36.0	38.0
58-59	37.100358426031775	38.0	38.0	38.0	36.0	38.0
60-61	37.11388829295922	38.0	38.0	38.0	36.0	38.0
62-63	37.11443757705909	38.0	38.0	38.0	36.0	38.0
64-65	37.11836059266328	38.0	38.0	38.0	36.0	38.0
66-67	37.0524637012163	38.0	38.0	38.0	36.0	38.0
68-69	37.06751138631335	38.0	38.0	38.0	36.0	38.0
70-71	37.08127604600819	38.0	38.0	38.0	36.0	38.0
72-73	37.09383575513009	38.0	38.0	38.0	36.0	38.0
74-75	37.0779202198043	38.0	38.0	38.0	36.0	38.0
76-77	37.043317651784825	38.0	38.0	38.0	36.0	38.0
78-79	37.04175975038902	38.0	38.0	38.0	36.0	38.0
80-81	37.03889651721106	38.0	38.0	38.0	36.0	38.0
82-83	36.9798083137657	38.0	38.0	38.0	36.0	38.0
84-85	36.9737497417628	38.0	38.0	38.0	36.0	38.0
86-87	36.96540870272875	38.0	38.0	38.0	36.0	38.0
88-89	36.94351148977114	38.0	38.0	38.0	36.0	38.0
90-91	36.91581646982688	38.0	38.0	38.0	35.0	38.0
92-93	36.89669635540385	38.0	38.0	38.0	35.0	38.0
94-95	36.89485709634023	38.0	38.0	38.0	35.0	38.0
96-97	36.867655231434526	38.0	38.0	38.0	35.0	38.0
98-99	36.888794477039276	38.0	38.0	38.0	35.0	38.0
100	35.38335949466532	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	6.0
21	53.0
22	191.0
23	708.0
24	1658.0
25	3415.0
26	6338.0
27	10876.0
28	17627.0
29	26237.0
30	34474.0
31	43412.0
32	55675.0
33	73882.0
34	108707.0
35	196005.0
36	599174.0
37	3557017.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.91533298686906	19.518982011662235	14.745292798561998	24.82039220290671
2	14.146837414919435	20.182119079419223	43.65011983296881	22.02092367269253
3	18.307254887385714	26.162316786387624	30.05011555381361	25.48031277241305
4	12.460465800872017	18.209583573454474	39.097071306950944	30.232879318722567
5	13.641323318953166	37.28827017117883	35.49739761125484	13.573008898613164
6	29.84351879871362	37.87131843874836	18.937285250399274	13.347877512138744
7	26.861990299985834	32.57275908111931	22.935209843527247	17.630040775367615
8	24.12385964015722	37.17824911090947	20.930566997018452	17.76732425191486
9	25.291349916174934	17.953663183933465	21.71792500702678	35.03706189286483
10-11	24.332413957090097	27.183057516940817	28.54473179674105	19.939796729228036
12-13	25.160158776650277	24.44383931688114	29.049688340533976	21.346313565934608
14-15	22.2024928111479	25.803496895864537	26.98827167050614	25.00573862248142
16-17	21.616298067958382	28.988986701811463	27.59689297147034	21.79782225875982
18-19	21.57066361282554	28.007212397874166	29.583776180419335	20.838347808880958
20-21	22.401508719473185	27.110101318651314	29.551433440824987	20.936956521050515
22-23	22.086818719043602	27.032894255424043	29.440802620634848	21.439484404897502
24-25	21.854183230498098	27.137635816377724	29.517864333099357	21.490316620024817
26-27	21.809456862585233	27.24653397212139	29.45592476483731	21.488084400456067
28-29	21.764668102044958	27.316987683085202	29.474534077769448	21.44381013710039
30-31	22.002154773271002	27.195915233181477	29.386365308488603	21.415564685058914
32-33	21.799373767339272	27.315089645888023	29.374672238588612	21.51086434818409
34-35	21.961049064068565	27.29733852266453	29.37258340098777	21.369029012279135
36-37	21.943917566963027	27.215398494706612	29.29194042157991	21.548743516750456
38-39	21.758096922924445	27.411825717840806	29.390641464682115	21.439435894552634
40-41	22.061964861541114	27.260922810587967	29.200127501832252	21.47698482603867
42-43	21.90374951265167	27.35002057094607	29.084280566164132	21.661949350238128
44-45	21.983135385002363	27.472438548886117	28.945278558847896	21.59914750726362
46-47	22.042901324858565	27.468503767811285	28.64314290632054	21.84545200100961
48-49	22.01562257150185	27.620921702061757	28.563482417604746	21.799973308831646
50-51	22.003966461650627	27.7469217464682	28.461855617213565	21.787256174667608
52-53	22.145017249868015	27.671371270510015	28.344446503606264	21.83916497601571
54-55	22.143239580819856	27.595589825180443	28.395665391852138	21.86550520214756
56-57	22.13720841217436	27.737389831073937	28.34158599972679	21.78381575702491
58-59	22.110717102896533	27.71907384572109	28.361383290078702	21.80882576130367
60-61	22.18006504739682	27.717677610196816	28.386885137224176	21.71537220518219
62-63	22.11264317480181	27.751199310277936	28.41578053343922	21.720376981481035
64-65	22.099301123517918	27.658544814084397	28.414284227997648	21.827869834400033
66-67	22.1952705543144	27.690282141469176	28.19431257545134	21.920134728765085
68-69	22.0954052608685	27.731530562862837	28.458835558786035	21.714228617482625
70-71	22.241584672629926	27.51402356756901	28.48376940583559	21.760622353965477
72-73	22.157954588842678	27.353231709747394	28.50127573080877	21.98753797060116
74-75	22.03284745740214	27.579254250132283	28.553105762634555	21.83479252983102
76-77	22.124024855736874	27.619731124172457	28.32809066476453	21.928153355326135
78-79	22.113842275937134	27.53719737487051	28.257680349987808	22.091279999204552
80-81	22.04365279214407	27.58374871167979	28.34661267956108	22.02598581661506
82-83	22.15958633630976	27.607753479896136	28.34555412724944	21.88710605654466
84-85	22.231071816221863	27.58735167571521	28.237342577962444	21.94423393010049
86-87	22.07278005704446	27.612429114466707	28.28826647767252	22.02652435081631
88-89	22.174538689443754	27.635695068647326	28.19596158935973	21.99380465254919
90-91	22.215829294824207	27.742549633533436	28.131562293476115	21.910058778166242
92-93	22.228951467373083	27.670319598070055	28.179745304188536	21.920983630368333
94-95	22.08226235042062	27.749438284558597	28.14370218331483	22.024597181705953
96-97	22.245247119653918	27.847653388044396	28.1867106549943	21.720388837307382
98-99	23.247914639247718	29.73882805402749	28.458222340685317	18.55503496603948
100	22.180431446135362	30.64470155721923	22.390395653024502	24.784471343620908
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	913.0
1	836.5
2	943.5
3	1247.5
4	1703.0
5	2491.5
6	3177.0
7	4053.0
8	5209.0
9	5851.5
10	5828.5
11	5844.5
12	6544.5
13	8151.5
14	11455.0
15	15465.5
16	18488.5
17	20135.0
18	20216.0
19	18862.0
20	16805.5
21	15198.0
22	15049.5
23	16806.0
24	20580.5
25	26504.0
26	34597.5
27	44191.5
28	53976.0
29	64919.5
30	76304.0
31	87044.0
32	99136.5
33	111073.0
34	123114.5
35	135129.0
36	147073.0
37	157831.5
38	165708.5
39	172581.0
40	180065.0
41	186607.5
42	189441.0
43	192214.0
44	195140.5
45	197159.0
46	197419.0
47	195981.5
48	193928.0
49	190348.5
50	185974.0
51	179975.5
52	171945.0
53	161614.0
54	149288.0
55	136928.5
56	124593.0
57	111026.0
58	97218.5
59	83988.5
60	70384.5
61	56673.0
62	44448.0
63	33986.0
64	25148.5
65	18434.5
66	13502.5
67	10153.0
68	7665.0
69	5562.0
70	4014.0
71	2820.0
72	1884.5
73	1199.5
74	740.0
75	464.0
76	296.5
77	177.0
78	106.0
79	63.5
80	40.0
81	28.0
82	17.0
83	10.0
84	9.0
85	8.5
86	7.0
87	7.0
88	5.5
89	4.0
90	3.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.011255513459419017
3	2.1117286040185774E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.167995975377913E-4
26-27	5.069273737824765E-4
28-29	0.002820394430576625
30-31	0.013465519497416924
32-33	0.007863009651077094
34-35	7.101942985601712E-4
36-37	1.4876518520627992E-4
38-39	7.041240009307239E-4
40-41	3.215411682555266E-5
42-43	0.0016693421015078467
44-45	7.257878264366157E-4
46-47	0.0016361128053968164
48-49	0.00353672931839867
50-51	0.0019569882724437253
52-53	0.0
54-55	0.0017234992406530943
56-57	0.04840827791691418
58-59	0.0687990710934322
60-61	0.04685194032838172
62-63	0.013644380729762687
64-65	0.0
66-67	0.0
68-69	2.000737919220795E-4
70-71	0.001519117620530115
72-73	0.005720921709904507
74-75	0.010388568665181138
76-77	0.019051769350958776
78-79	0.013623892920620204
80-81	0.006689711483893219
82-83	0.03761074256834609
84-85	0.026793965756706253
86-87	0.008540156362499218
88-89	0.0027108728226776973
90-91	0.0035942704474412556
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	24.0
20-21	163.0
22-23	343.0
24-25	374.0
26-27	814.0
28-29	1857.0
30-31	5577.0
32-33	7129.0
34-35	9965.0
36-37	17207.0
38-39	21679.0
40-41	21857.0
42-43	25260.0
44-45	30942.0
46-47	32238.0
48-49	30723.0
50-51	27827.0
52-53	26765.0
54-55	29019.0
56-57	30198.0
58-59	30954.0
60-61	30992.0
62-63	30074.0
64-65	32013.0
66-67	34162.0
68-69	35440.0
70-71	35671.0
72-73	34001.0
74-75	33506.0
76-77	35605.0
78-79	37432.0
80-81	37786.0
82-83	35558.0
84-85	34868.0
86-87	36368.0
88-89	37888.0
90-91	39302.0
92-93	38963.0
94-95	46005.0
96-97	147113.0
98-99	325524.0
100-101	3266271.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.85087475419485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.49937988207468	80.38300562106639
2	6.205115656357375	10.902496767238906
3	1.341511104121915	3.5355877196872796
4	0.4317809074190769	1.517293216757037
5	0.20147038294059794	0.8849674689197072
6	0.102628697698396	0.5409612520612748
7	0.06456488166182789	0.39704569326748573
8	0.039290525294678046	0.27613656133514286
9	0.02354524057291181	0.18616229825654365
>10	0.08882516377477974	1.2056922462562247
>50	0.0012069430577387398	0.07367390901324562
>100	6.806150258955439E-4	0.09697724614074933
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1117286040185774E-5
2	0.0	0.0	0.0	0.0	2.1117286040185774E-5
3	0.0	0.0	0.0	0.0	2.1117286040185774E-5
4	0.0	2.1117286040185774E-5	0.0	0.0	2.1117286040185774E-5
5	0.0	4.223457208037155E-5	0.0	0.0	2.1117286040185774E-5
6	2.1117286040185774E-5	4.223457208037155E-5	0.0	0.0	2.1117286040185774E-5
7	2.1117286040185774E-5	4.223457208037155E-5	0.0	0.0	2.1117286040185774E-5
8	2.1117286040185774E-5	4.223457208037155E-5	0.0	0.0	2.1117286040185774E-5
9	2.1117286040185774E-5	6.335185812055732E-5	0.0	2.1117286040185774E-5	2.1117286040185774E-5
10-11	2.1117286040185774E-5	6.335185812055732E-5	0.0	4.223457208037155E-5	2.1117286040185774E-5
12-13	2.1117286040185774E-5	6.335185812055732E-5	0.0	4.223457208037155E-5	2.1117286040185774E-5
14-15	2.1117286040185774E-5	9.502778718083597E-5	0.0	4.223457208037155E-5	2.1117286040185774E-5
16-17	6.335185812055732E-5	1.0558643020092886E-4	0.0	4.223457208037155E-5	2.1117286040185774E-5
18-19	6.335185812055732E-5	1.0558643020092886E-4	0.0	4.223457208037155E-5	2.1117286040185774E-5
20-21	6.335185812055732E-5	1.0558643020092886E-4	0.0	6.335185812055732E-5	2.1117286040185774E-5
22-23	6.335185812055732E-5	1.2670371624111463E-4	0.0	6.335185812055732E-5	3.167592906027866E-5
24-25	6.335185812055732E-5	1.5837964530139332E-4	0.0	9.502778718083597E-5	4.223457208037155E-5
26-27	6.335185812055732E-5	1.689382883214862E-4	0.0	1.0558643020092886E-4	4.223457208037155E-5
28-29	6.335185812055732E-5	1.689382883214862E-4	0.0	2.2173150342195063E-4	4.223457208037155E-5
30-31	6.335185812055732E-5	2.322901464420435E-4	0.0	6.651945102658518E-4	4.223457208037155E-5
32-33	6.335185812055732E-5	2.322901464420435E-4	0.0	0.0017527347413354192	4.223457208037155E-5
34-35	6.335185812055732E-5	2.5340743248222926E-4	0.0	0.0031359169769675874	4.223457208037155E-5
36-37	1.0558643020092886E-4	2.7452471852241507E-4	0.0	0.005310997439106722	4.223457208037155E-5
38-39	1.0558643020092886E-4	2.7452471852241507E-4	0.0	0.008499707631174774	7.39105011406502E-5
40-41	1.0558643020092886E-4	3.167592906027866E-4	0.0	0.01203685304290589	8.44691441607431E-5
42-43	1.0558643020092886E-4	3.167592906027866E-4	0.0	0.015531763882556636	8.44691441607431E-5
44-45	1.0558643020092886E-4	3.378765766429724E-4	0.0	0.01909002658032794	8.44691441607431E-5
46-47	1.0558643020092886E-4	3.5899386268315814E-4	0.0	0.023651360365008065	8.44691441607431E-5
48-49	1.0558643020092886E-4	3.5899386268315814E-4	0.0	0.02812822500552745	8.44691441607431E-5
50-51	1.0558643020092886E-4	4.012284347635297E-4	0.0	0.03284793843550897	8.44691441607431E-5
52-53	1.0558643020092886E-4	4.117870777836226E-4	0.0	0.03744094814924938	8.44691441607431E-5
54-55	1.0558643020092886E-4	4.540216498639941E-4	0.0	0.04183334364560802	8.44691441607431E-5
56-57	1.0558643020092886E-4	4.64580292884087E-4	0.0	0.04661640893371009	8.44691441607431E-5
58-59	1.0558643020092886E-4	4.64580292884087E-4	0.0	0.05149450200899301	8.44691441607431E-5
60-61	1.1614507322102175E-4	4.64580292884087E-4	0.0	0.05663656115977824	8.44691441607431E-5
62-63	1.2670371624111463E-4	4.64580292884087E-4	0.0	0.06186308945472423	1.0558643020092886E-4
64-65	1.2670371624111463E-4	4.856975789242728E-4	0.0	0.06759643261463466	1.0558643020092886E-4
66-67	1.2670371624111463E-4	4.856975789242728E-4	0.0	0.07232670468763627	1.0558643020092886E-4
68-69	1.2670371624111463E-4	4.856975789242728E-4	0.0	0.07726814962103976	1.0558643020092886E-4
70-71	1.2670371624111463E-4	5.068148649644585E-4	0.0	0.08158663461625773	1.0558643020092886E-4
72-73	1.9005557436167195E-4	5.4904943704483E-4	0.0	0.08539830474651126	1.2670371624111463E-4
74-75	1.9005557436167195E-4	5.807253661051087E-4	0.0	0.08841807665025783	1.2670371624111463E-4
76-77	1.9005557436167195E-4	5.912840091252017E-4	0.0	0.09084656454487919	1.2670371624111463E-4
78-79	1.9005557436167195E-4	5.912840091252017E-4	0.0	0.09245147828393332	1.2670371624111463E-4
80-81	1.9005557436167195E-4	5.912840091252017E-4	0.0	0.09301108636399824	1.2670371624111463E-4
82-83	1.9005557436167195E-4	5.912840091252017E-4	0.0	0.09363404630218372	1.2670371624111463E-4
84-85	1.9005557436167195E-4	5.912840091252017E-4	0.0	0.09385577780560567	1.2670371624111463E-4
86-87	1.9005557436167195E-4	6.124012951653874E-4	0.0	0.09396136423580659	1.2670371624111463E-4
88	2.1117286040185773E-4	6.335185812055732E-4	0.0	0.09397192287882669	1.2670371624111463E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6745	0.0	49.152924	1
GTATCAA	12265	0.0	38.89928	1
TCAACGC	14440	0.0	32.46327	4
ATCAACG	14575	0.0	32.281815	3
CAACGCA	14730	0.0	31.765156	5
TATCAAC	15145	0.0	31.502127	2
AACGCAG	15270	0.0	30.641829	6
ACGCAGA	17490	0.0	26.926352	7
CGCAGAG	17770	0.0	26.453175	8
TGGTATC	3130	0.0	23.322319	2
GCAGAGT	20470	0.0	22.963995	9
GTGGTAT	3600	0.0	22.570745	1
GAGTACT	12440	0.0	20.377855	12-13
CAGAGTA	20420	0.0	19.520384	10-11
GTACATG	14435	0.0	19.204838	1
TACATGG	14410	0.0	18.846159	2
AGAGTAC	18930	0.0	18.842154	10-11
ACATGGG	14080	0.0	18.729406	3
GTACTTT	13540	0.0	18.513977	14-15
CATGGGG	9955	0.0	17.107353	4
>>END_MODULE