FastQCFastQC Report
Fri 27 May 2016
SRR2139460_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139460_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5294793
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74100.051.7755241
GTATCAA143500.039.8024061
TCAACGC169800.033.1251534
ATCAACG172950.032.6966823
CAACGCA174450.032.24225
TATCAAC176000.032.207052
AACGCAG179850.031.2981456
ACGCAGA204550.027.5399277
CGCAGAG211850.026.5909468
TGGTATC35100.025.9719832
GTGGTAT37500.025.577111
GCAGAGT242400.023.2040089
GAGTACT145450.020.82039512-13
GTACATG167650.020.6938721
TACATGG168250.020.2348942
ACATGGG168600.019.6783333
CAGAGTA240100.019.57588410-11
AGAGTAC222400.019.20111810-11
GTACTTT161300.018.85520214-15
CATGGGG123650.018.097574
Produced by FastQC (version 0.11.5)