##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139460_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5294793
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.624959842622744	31.0	18.0	33.0	18.0	33.0
2	31.47862324362822	33.0	32.0	33.0	27.0	33.0
3	32.49247458776953	33.0	33.0	33.0	31.0	34.0
4	32.63557083345846	33.0	33.0	34.0	31.0	34.0
5	33.18633854052462	33.0	33.0	34.0	33.0	34.0
6	37.06318943913388	38.0	38.0	38.0	36.0	38.0
7	37.18449918627603	38.0	38.0	38.0	36.0	38.0
8	37.1381702740787	38.0	38.0	38.0	36.0	38.0
9	37.36882631672287	38.0	38.0	38.0	37.0	38.0
10-11	37.4156264465863	38.0	38.0	38.0	37.0	38.0
12-13	37.538351263212746	38.0	38.0	38.0	37.5	38.0
14-15	37.51913823637676	38.0	38.0	38.0	37.0	38.0
16-17	37.538915685655695	38.0	38.0	38.0	37.5	38.0
18-19	37.54942931668906	38.0	38.0	38.0	38.0	38.0
20-21	37.5562742069675	38.0	38.0	38.0	38.0	38.0
22-23	37.5666093164928	38.0	38.0	38.0	38.0	38.0
24-25	37.574375768835395	38.0	38.0	38.0	38.0	38.0
26-27	37.55134324188934	38.0	38.0	38.0	38.0	38.0
28-29	37.52968886273574	38.0	38.0	38.0	38.0	38.0
30-31	37.52350317698988	38.0	38.0	38.0	38.0	38.0
32-33	37.43257317688041	38.0	38.0	38.0	37.0	38.0
34-35	37.48102566675168	38.0	38.0	38.0	37.0	38.0
36-37	37.468530308675966	38.0	38.0	38.0	37.5	38.0
38-39	37.454251261306936	38.0	38.0	38.0	37.5	38.0
40-41	37.43612600432235	38.0	38.0	38.0	37.0	38.0
42-43	37.39780541669201	38.0	38.0	38.0	37.0	38.0
44-45	37.35809671477226	38.0	38.0	38.0	37.0	38.0
46-47	37.322291263984695	38.0	38.0	38.0	37.0	38.0
48-49	37.204470663164244	38.0	38.0	38.0	37.0	38.0
50-51	37.1914973492742	38.0	38.0	38.0	37.0	38.0
52-53	37.19814179426838	38.0	38.0	38.0	37.0	38.0
54-55	37.18078287046036	38.0	38.0	38.0	37.0	38.0
56-57	37.12517589341888	38.0	38.0	38.0	36.0	38.0
58-59	37.10574438419492	38.0	38.0	38.0	36.0	38.0
60-61	37.12149719930909	38.0	38.0	38.0	36.0	38.0
62-63	37.1250889907612	38.0	38.0	38.0	36.0	38.0
64-65	37.131655084910825	38.0	38.0	38.0	36.0	38.0
66-67	37.06613644115029	38.0	38.0	38.0	36.0	38.0
68-69	37.08030508323647	38.0	38.0	38.0	36.0	38.0
70-71	37.09661022853072	38.0	38.0	38.0	36.0	38.0
72-73	37.10964976139857	38.0	38.0	38.0	36.0	38.0
74-75	37.09475271728214	38.0	38.0	38.0	36.0	38.0
76-77	37.060747538433475	38.0	38.0	38.0	36.0	38.0
78-79	37.05915748503273	38.0	38.0	38.0	36.0	38.0
80-81	37.05753120111654	38.0	38.0	38.0	36.0	38.0
82-83	36.996060564040576	38.0	38.0	38.0	36.0	38.0
84-85	36.99319063702637	38.0	38.0	38.0	36.0	38.0
86-87	36.983644167172926	38.0	38.0	38.0	36.0	38.0
88-89	36.96161486412179	38.0	38.0	38.0	36.0	38.0
90-91	36.93585006528356	38.0	38.0	38.0	35.0	38.0
92-93	36.91987827298901	38.0	38.0	38.0	35.0	38.0
94-95	36.91576336287365	38.0	38.0	38.0	35.0	38.0
96-97	36.88676721887664	38.0	38.0	38.0	35.0	38.0
98-99	36.90892880044825	38.0	38.0	38.0	35.0	38.0
100	35.418957432756464	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	10.0
21	66.0
22	216.0
23	723.0
24	1720.0
25	3462.0
26	6596.0
27	11345.0
28	18541.0
29	27958.0
30	37381.0
31	47658.0
32	60542.0
33	80215.0
34	118691.0
35	217494.0
36	682431.0
37	3979739.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.393138504187036	19.13732982573634	14.515298331776144	24.954233338300476
2	14.433912103168044	20.1053779790434	43.095683544961304	22.365026372827252
3	18.48892269989076	25.859656054477682	30.029904857452376	25.621516388179177
4	12.754606270726732	17.944761957644047	38.570780765178164	30.729851006451053
5	13.905019516343698	37.00813232925253	35.23899801182029	13.847850142583479
6	30.253080715336743	37.221530662294064	18.825778458194684	13.6996101641745
7	27.077659126617416	32.35299283654715	22.800494750219695	17.768853286615737
8	24.469700704069073	36.55298705728439	20.92217769419881	18.055134544447725
9	25.562547959854143	17.503498248184584	21.49092514098285	35.44302865097843
10-11	24.54449116329949	26.81001882415422	28.370948590435923	20.274541422110364
12-13	25.38692258601989	24.1238514895672	28.870665954268652	21.61855997014425
14-15	22.49476986163576	25.453138583510253	26.76934679032778	25.282744764526203
16-17	21.88746566674089	28.61889029467252	27.407936438686082	22.085707599900505
18-19	21.86023136315244	27.64033457020888	29.407721888277788	21.09171217836089
20-21	22.654419542264545	26.662964249704824	29.438747647547153	21.243868560483474
22-23	22.464235237633126	26.50387843899937	29.29877399813052	21.733112325236988
24-25	22.18308242961212	26.643759530604562	29.399104177476598	21.774053862306715
26-27	22.10323807444033	26.78378777125398	29.363407837639688	21.749566316666
28-29	22.049492499690576	26.796422602305505	29.41331584820058	21.74076904980334
30-31	22.29953332510707	26.658695702534587	29.28793193193761	21.75383904042073
32-33	22.08695983169077	26.869321639532178	29.195163315875135	21.848555212901914
34-35	22.26357565759204	26.790110339423457	29.275607597302372	21.670706405682132
36-37	22.216997898515263	26.710500877965465	29.217668836145844	21.85483238737343
38-39	22.06753310495978	26.88314053842159	29.29320913267423	21.7561172239444
40-41	22.32456632805708	26.7338929773705	29.117363407517015	21.8241772870554
42-43	22.206356000487798	26.828247865422426	28.933245648024336	22.03215048606544
44-45	22.253125521681444	26.956046704566855	28.81890251924889	21.971925254502807
46-47	22.369464054785894	26.93127050730841	28.562455793508	22.136809644397694
48-49	22.31050033692556	27.10551837699553	28.403485872311624	22.180495413767282
50-51	22.306454688195974	27.224331759799465	28.337301198149117	22.131912353855444
52-53	22.45818300508367	27.153144365819372	28.21413789375885	22.174534735338113
54-55	22.41750999136192	27.079474532771847	28.23326470783029	22.269750768035937
56-57	22.406116012312427	27.181659427353033	28.246121991858043	22.166102568476497
58-59	22.377971035700714	27.140252924373563	28.29078056292141	22.190995477004314
60-61	22.446686028484354	27.129779356810463	28.323524773202784	22.1000098415024
62-63	22.39828518405242	27.14627255620584	28.32824106953091	22.127201190210826
64-65	22.384761773454994	27.070308583174572	28.319201427186734	22.225728216183697
66-67	22.43191951487715	26.988670196763948	28.240711539905345	22.33869874845356
68-69	22.349349533595298	27.036975159954718	28.45369796738741	22.15997733906258
70-71	22.508405412254998	26.89336498858471	28.40525151404581	22.192978085114476
72-73	22.436591779923756	26.807303755048533	28.355074355137933	22.401030109889778
74-75	22.291886753913097	27.04384099779501	28.41219068233617	22.252081565955724
76-77	22.408325312059464	27.094487745386065	28.148751608105865	22.34843533444861
78-79	22.435264808289997	27.06695929677249	28.04924876265516	22.44852713228235
80-81	22.373138388112604	27.142869160686793	28.1194684433826	22.364524007817998
82-83	22.489356682951637	27.14160852036422	28.07421168524833	22.294823111435818
84-85	22.520084551122917	27.184965416102315	27.990131432991493	22.30481859978327
86-87	22.387473432906525	27.203861097718306	28.01958759352373	22.389077875851438
88-89	22.482268337698848	27.202492394886917	27.940112292075515	22.375126975338716
90-91	22.491379526683673	27.254327261408147	27.942280324466996	22.312012887441192
92-93	22.552007336538207	27.218761569772433	27.927502979783913	22.301728113905448
94-95	22.42760849319835	27.280008138983984	27.92391494761733	22.368468420200333
96-97	22.553870532668522	27.36877543386068	27.934974429939725	22.142379603531076
98-99	23.625145437445976	29.265000173084765	28.138733648444497	18.971120741024762
100	22.493583956793525	30.19654837252744	22.057706146616844	25.252161524062185
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1128.0
1	1015.0
2	1223.5
3	1629.0
4	2032.0
5	2906.0
6	3826.0
7	5122.0
8	6657.0
9	7452.5
10	7422.0
11	7273.5
12	7958.5
13	9798.0
14	13499.5
15	17883.0
16	21424.5
17	23642.5
18	23582.0
19	21602.0
20	18875.5
21	16581.5
22	15942.0
23	17543.5
24	21364.0
25	27562.5
26	36164.0
27	45921.5
28	55996.5
29	67421.5
30	79250.0
31	91201.0
32	104763.0
33	118217.5
34	131475.0
35	143473.0
36	155072.5
37	165892.5
38	174844.0
39	183665.5
40	192447.5
41	200417.5
42	204595.5
43	207817.5
44	211359.5
45	214938.5
46	216425.5
47	215867.0
48	215654.5
49	215190.5
50	214715.5
51	210403.0
52	202128.5
53	191676.0
54	179646.5
55	167842.5
56	155072.5
57	139870.5
58	124194.0
59	108498.5
60	91452.5
61	74346.5
62	59177.0
63	45853.5
64	34434.0
65	25624.0
66	19082.5
67	14482.5
68	11023.0
69	8240.0
70	6048.5
71	4247.5
72	2883.5
73	1905.0
74	1208.5
75	749.0
76	465.0
77	276.0
78	161.5
79	102.0
80	68.0
81	50.0
82	37.5
83	25.0
84	15.0
85	11.0
86	11.0
87	9.5
88	6.0
89	4.0
90	3.0
91	1.5
92	1.0
93	1.0
94	1.5
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.009783196434685927
3	1.8886479603640787E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.5888683127105627E-4
26-27	3.872579295547601E-4
28-29	0.002853220151821545
30-31	0.013292628518965774
32-33	0.008057271578717743
34-35	7.779006396999506E-4
36-37	1.3319787743473828E-4
38-39	8.122912471222193E-4
40-41	9.60476396292561E-6
42-43	0.0017572800394712131
44-45	6.413178966172229E-4
46-47	0.001449051165213373
48-49	0.003235959241909461
50-51	0.00224544212507053
52-53	0.0
54-55	0.00145945331697001
56-57	0.04685296956290396
58-59	0.06459291248778608
60-61	0.044750565247281084
62-63	0.013569464827148964
64-65	0.0
66-67	0.0
68-69	3.08007668328846E-4
70-71	0.0016395308241439238
72-73	0.005351804374359473
74-75	0.009595946323329524
76-77	0.017887871429108915
78-79	0.013360505988905116
80-81	0.0064603678304637965
82-83	0.03691251032819741
84-85	0.026535257880748654
86-87	0.008136980116221468
88-89	0.002180086830410229
90-91	0.003310585721549816
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	29.0
20-21	142.0
22-23	406.0
24-25	395.0
26-27	1086.0
28-29	2386.0
30-31	7339.0
32-33	9471.0
34-35	13336.0
36-37	21519.0
38-39	26521.0
40-41	26491.0
42-43	30944.0
44-45	37859.0
46-47	39486.0
48-49	36616.0
50-51	32922.0
52-53	31686.0
54-55	34774.0
56-57	36277.0
58-59	36637.0
60-61	36271.0
62-63	35857.0
64-65	38032.0
66-67	40331.0
68-69	41358.0
70-71	41975.0
72-73	39665.0
74-75	39673.0
76-77	42198.0
78-79	44016.0
80-81	44134.0
82-83	41439.0
84-85	40910.0
86-87	42902.0
88-89	44247.0
90-91	45623.0
92-93	44806.0
94-95	52771.0
96-97	165850.0
98-99	363987.0
100-101	3582426.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.50089346817224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.44997000380539	80.01954082970522
2	6.180617174214888	10.816190498570652
3	1.3680928886995254	3.5912805032598354
4	0.45919862082214125	1.607211584051592
5	0.20792783510281068	0.909693567419936
6	0.1052209641770021	0.5524157026242136
7	0.059403128842880445	0.3638478793989894
8	0.03942284659600765	0.2759627440167489
9	0.027783682455025386	0.21879873347856327
>10	0.09906031091019578	1.3530085433565435
>50	0.0025687937963347466	0.15331358157365096
>100	7.009121393911483E-4	0.11890942291981207
>500	3.283843838940545E-5	0.019826409624290275
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.8886479603640783E-5	0.0
2	0.0	7.554591841456313E-5	0.0	1.8886479603640783E-5	0.0
3	0.0	7.554591841456313E-5	0.0	1.8886479603640783E-5	0.0
4	0.0	9.443239801820392E-5	0.0	1.8886479603640783E-5	0.0
5	0.0	9.443239801820392E-5	0.0	1.8886479603640783E-5	0.0
6	0.0	9.443239801820392E-5	0.0	1.8886479603640783E-5	1.8886479603640783E-5
7	0.0	9.443239801820392E-5	0.0	1.8886479603640783E-5	1.8886479603640783E-5
8	0.0	1.3220535722548548E-4	0.0	1.8886479603640783E-5	1.8886479603640783E-5
9	0.0	1.3220535722548548E-4	0.0	1.8886479603640783E-5	1.8886479603640783E-5
10-11	0.0	1.3220535722548548E-4	0.0	1.8886479603640783E-5	5.6659438810922346E-5
12-13	1.8886479603640783E-5	1.3220535722548548E-4	0.0	3.7772959207281566E-5	7.554591841456313E-5
14-15	1.8886479603640783E-5	1.6997831643276705E-4	0.0	3.7772959207281566E-5	9.443239801820392E-5
16-17	1.8886479603640783E-5	1.9830803583822823E-4	0.0	3.7772959207281566E-5	9.443239801820392E-5
18-19	1.8886479603640783E-5	2.360809950455098E-4	0.0	4.721619900910196E-5	1.133188776218447E-4
20-21	1.8886479603640783E-5	2.6441071445097096E-4	0.0	5.665943881092235E-5	1.133188776218447E-4
22-23	1.8886479603640783E-5	2.6441071445097096E-4	0.0	5.665943881092235E-5	1.4164859702730587E-4
24-25	1.8886479603640783E-5	3.1162691346007295E-4	0.0	8.498915821638353E-5	1.6997831643276705E-4
26-27	1.8886479603640783E-5	3.399566328655341E-4	0.0	1.6997831643276705E-4	1.6997831643276705E-4
28-29	1.8886479603640783E-5	3.399566328655341E-4	0.0	3.9661607167645646E-4	1.6997831643276705E-4
30-31	1.8886479603640783E-5	3.588431124691749E-4	0.0	0.0011520752558220877	1.7942155623458744E-4
32-33	1.8886479603640783E-5	3.588431124691749E-4	0.0	0.0025685612260951465	2.0775127564004862E-4
34-35	1.8886479603640783E-5	4.2494579108191767E-4	0.0	0.004929371176550245	2.0775127564004862E-4
36-37	1.8886479603640783E-5	5.099349492983012E-4	0.0	0.008376153704214687	2.0775127564004862E-4
38-39	1.8886479603640783E-5	5.665943881092235E-4	0.0	0.014032654345505102	2.0775127564004862E-4
40-41	1.8886479603640783E-5	6.043673473165051E-4	0.0	0.019868576543030106	2.0775127564004862E-4
42-43	1.8886479603640783E-5	6.326970667219663E-4	0.0	0.02574227169976239	2.0775127564004862E-4
44-45	1.8886479603640783E-5	6.799132657310682E-4	0.0	0.03250363139786579	2.0775127564004862E-4
46-47	1.8886479603640783E-5	7.176862249383498E-4	0.0	0.039756039565663856	2.1719451544186902E-4
48-49	1.8886479603640783E-5	7.176862249383498E-4	0.0	0.047924441994238495	2.455242348473302E-4
50-51	1.8886479603640783E-5	8.121186229565538E-4	0.0	0.05537515819787478	2.455242348473302E-4
52-53	1.8886479603640783E-5	8.121186229565538E-4	0.0	0.06355300386625123	2.64410714450971E-4
54-55	1.8886479603640783E-5	8.215618627583741E-4	0.0	0.07084318499325659	2.927404338564321E-4
56-57	1.8886479603640783E-5	8.498915821638353E-4	0.0	0.07541371305733766	3.0218367365825253E-4
58-59	1.8886479603640783E-5	8.498915821638353E-4	0.0	0.08014477619804966	3.1162691346007295E-4
60-61	1.8886479603640783E-5	8.498915821638353E-4	0.0	0.08550853640548364	3.210701532618933E-4
62-63	1.8886479603640783E-5	8.498915821638353E-4	0.0	0.09074953449549397	3.210701532618933E-4
64-65	1.8886479603640783E-5	0.001038756378200243	0.0	0.09566946243224239	3.399566328655341E-4
66-67	1.8886479603640783E-5	0.0010765293374075248	0.0	0.1009104605222527	3.399566328655341E-4
68-69	1.8886479603640783E-5	0.0010765293374075248	0.0	0.10603813973464118	3.399566328655341E-4
70-71	1.8886479603640783E-5	0.0010765293374075248	0.0	0.11031592736486583	3.493998726673545E-4
72-73	1.8886479603640783E-5	0.0010765293374075248	0.0	0.11412155300499943	3.588431124691749E-4
74-75	1.8886479603640783E-5	0.0011237455364166266	0.0	0.11730392481821292	3.588431124691749E-4
76-77	1.8886479603640783E-5	0.001152075255822088	0.0	0.12034464803439908	3.6828635227099526E-4
78-79	1.8886479603640783E-5	0.0011615184956239083	0.0	0.12216719331615042	3.9661607167645646E-4
80-81	1.8886479603640783E-5	0.0011709617354257287	0.0	0.12274323094406145	4.2494579108191767E-4
82-83	1.8886479603640783E-5	0.0011709617354257287	0.0	0.12316817673514338	5.004917094964807E-4
84-85	1.8886479603640783E-5	0.0011898482150293694	0.0	0.12337592801078343	5.288214289019419E-4
86-87	1.8886479603640783E-5	0.0012087346946330101	0.0	0.12347036040880163	5.288214289019419E-4
88	1.8886479603640783E-5	0.0012653941334439325	0.0	0.12347980364860345	5.288214289019419E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7410	0.0	51.775524	1
GTATCAA	14350	0.0	39.802406	1
TCAACGC	16980	0.0	33.125153	4
ATCAACG	17295	0.0	32.696682	3
CAACGCA	17445	0.0	32.2422	5
TATCAAC	17600	0.0	32.20705	2
AACGCAG	17985	0.0	31.298145	6
ACGCAGA	20455	0.0	27.539927	7
CGCAGAG	21185	0.0	26.590946	8
TGGTATC	3510	0.0	25.971983	2
GTGGTAT	3750	0.0	25.57711	1
GCAGAGT	24240	0.0	23.204008	9
GAGTACT	14545	0.0	20.820395	12-13
GTACATG	16765	0.0	20.693872	1
TACATGG	16825	0.0	20.234894	2
ACATGGG	16860	0.0	19.678333	3
CAGAGTA	24010	0.0	19.575884	10-11
AGAGTAC	22240	0.0	19.201118	10-11
GTACTTT	16130	0.0	18.855202	14-15
CATGGGG	12365	0.0	18.09757	4
>>END_MODULE