FastQCFastQC Report
Fri 27 May 2016
SRR2139459_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139459_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5182161
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68250.050.7094041
GTATCAA129000.039.458071
TCAACGC153150.032.4439284
ATCAACG154450.032.338263
TATCAAC157350.031.965372
CAACGCA158000.031.4480255
AACGCAG162700.030.5925436
ACGCAGA186300.026.717167
CGCAGAG191600.025.9781138
GTGGTAT34850.024.2397941
TGGTATC33850.022.9186732
GCAGAGT220050.022.6781859
GTACATG161850.021.2237051
GAGTACT127750.020.77978112-13
TACATGG162150.020.4667742
ACATGGG166000.019.4184553
CAGAGTA218000.019.28376810-11
GTACTTT138650.019.084114-15
AGAGTAC202050.018.3852510-11
CATGGGG115900.017.1039164
Produced by FastQC (version 0.11.5)