##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139459_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5182161
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.303104052537154	32.0	25.0	33.0	18.0	34.0
2	31.621897891632468	33.0	32.0	33.0	28.0	33.0
3	32.537998722926595	33.0	33.0	33.0	31.0	34.0
4	32.71346721956342	33.0	33.0	34.0	31.0	34.0
5	33.19651666553779	33.0	33.0	34.0	33.0	34.0
6	37.09365127791283	38.0	38.0	38.0	36.0	38.0
7	37.24014460376665	38.0	38.0	38.0	36.0	38.0
8	37.17648448205295	38.0	38.0	38.0	36.0	38.0
9	37.380260088407134	38.0	38.0	38.0	37.0	38.0
10-11	37.427230744085335	38.0	38.0	38.0	37.0	38.0
12-13	37.54285683134893	38.0	38.0	38.0	37.5	38.0
14-15	37.53101843034209	38.0	38.0	38.0	37.5	38.0
16-17	37.548122202301315	38.0	38.0	38.0	38.0	38.0
18-19	37.550172891193455	38.0	38.0	38.0	38.0	38.0
20-21	37.55647971444132	38.0	38.0	38.0	38.0	38.0
22-23	37.565210908132784	38.0	38.0	38.0	38.0	38.0
24-25	37.57364524905935	38.0	38.0	38.0	38.0	38.0
26-27	37.54678671166963	38.0	38.0	38.0	38.0	38.0
28-29	37.52588307821177	38.0	38.0	38.0	38.0	38.0
30-31	37.51855231393702	38.0	38.0	38.0	38.0	38.0
32-33	37.434786060549754	38.0	38.0	38.0	37.0	38.0
34-35	37.482519520927255	38.0	38.0	38.0	37.5	38.0
36-37	37.469767252499686	38.0	38.0	38.0	37.5	38.0
38-39	37.46084502723788	38.0	38.0	38.0	37.5	38.0
40-41	37.4493279279812	38.0	38.0	38.0	38.0	38.0
42-43	37.41497226438608	38.0	38.0	38.0	37.0	38.0
44-45	37.38074249210037	38.0	38.0	38.0	37.0	38.0
46-47	37.352965312085345	38.0	38.0	38.0	37.0	38.0
48-49	37.24373461579519	38.0	38.0	38.0	37.0	38.0
50-51	37.238746069915436	38.0	38.0	38.0	37.0	38.0
52-53	37.24575787078194	38.0	38.0	38.0	37.0	38.0
54-55	37.228855452008716	38.0	38.0	38.0	37.0	38.0
56-57	37.17807874303821	38.0	38.0	38.0	37.0	38.0
58-59	37.16071308151451	38.0	38.0	38.0	37.0	38.0
60-61	37.17498932291045	38.0	38.0	38.0	37.0	38.0
62-63	37.1779053680431	38.0	38.0	38.0	37.0	38.0
64-65	37.18387778178346	38.0	38.0	38.0	37.0	38.0
66-67	37.12117884763854	38.0	38.0	38.0	36.0	38.0
68-69	37.13722284679508	38.0	38.0	38.0	36.0	38.0
70-71	37.15288601136612	38.0	38.0	38.0	36.0	38.0
72-73	37.164644538144	38.0	38.0	38.0	36.0	38.0
74-75	37.14788591637817	38.0	38.0	38.0	36.0	38.0
76-77	37.11565733331696	38.0	38.0	38.0	36.0	38.0
78-79	37.11340722447815	38.0	38.0	38.0	36.0	38.0
80-81	37.11113537481597	38.0	38.0	38.0	36.0	38.0
82-83	37.05246767500446	38.0	38.0	38.0	36.0	38.0
84-85	37.04813319512959	38.0	38.0	38.0	36.0	38.0
86-87	37.04049435135163	38.0	38.0	38.0	36.0	38.0
88-89	37.019054054306736	38.0	38.0	38.0	36.0	38.0
90-91	36.992514731610584	38.0	38.0	38.0	36.0	38.0
92-93	36.977899212738016	38.0	38.0	38.0	36.0	38.0
94-95	36.97070731331278	38.0	38.0	38.0	35.5	38.0
96-97	36.94648870569099	38.0	38.0	38.0	35.0	38.0
98-99	36.965728388928	38.0	38.0	38.0	35.0	38.0
100	35.536893388872755	38.0	36.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	14.0
21	50.0
22	242.0
23	672.0
24	1681.0
25	3460.0
26	6430.0
27	10861.0
28	17300.0
29	26054.0
30	34803.0
31	45176.0
32	57593.0
33	76885.0
34	111839.0
35	203112.0
36	621681.0
37	3964307.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.30549012274995	19.38341938816644	14.551979376943326	24.759111112140282
2	14.396764680906909	20.122916650583996	43.04516400466269	22.43515466384641
3	18.44996561276812	25.89688538901569	29.996839153433434	25.656309844782747
4	12.718902403842721	17.942534012355075	38.56009105081837	30.778472532983827
5	13.84067380384361	37.205212265693795	35.24182286115773	13.712291069304872
6	30.279337133678403	37.42980968750296	18.71327424987375	13.577578928944895
7	27.107629423323594	32.43585060363813	22.687195554132725	17.769324418905548
8	24.44773522088565	36.77139710634231	20.799701128544637	17.9811665442274
9	25.45598255245254	17.65089892035388	21.440572764914094	35.45254576227948
10-11	24.536887217514085	26.95405449579818	28.332591364876546	20.17646692181119
12-13	25.313059551797018	24.212900757039392	28.777444390477253	21.696595300686337
14-15	22.456288023471288	25.589826329208993	26.630637295908024	25.3232483514117
16-17	21.921655849750714	28.780232802493018	27.26781356272026	22.03029778503601
18-19	21.903950880723315	27.85631322531276	29.09376030578749	21.145975588176437
20-21	22.761986113189575	26.936124396397105	29.044917308321132	21.256972182092184
22-23	22.518688397856266	26.798689243107464	28.895751676776715	21.786870682259558
24-25	22.21172660218953	26.956891950663575	29.010533864875534	21.820847582271362
26-27	22.14650679474949	27.039363368910287	28.993234443587152	21.82089539275307
28-29	22.094164016462557	27.103669999753844	29.029733287062353	21.772432696721246
30-31	22.359919013985515	26.984289227153944	28.89380975976732	21.761981999093223
32-33	22.13596135027887	27.154314240097094	28.798934024994143	21.910790384629898
34-35	22.273785141483987	27.110854449059683	28.86988568575123	21.7454747237051
36-37	22.26246710176385	27.018259262507748	28.83698013591228	21.88229349981612
38-39	22.110648942691224	27.215772120985694	28.904560538667745	21.769018397655334
40-41	22.384196079582935	27.031084438943797	28.744519988886523	21.84019949258674
42-43	22.247741254588437	27.163461301093445	28.5717840538864	22.017013390431718
44-45	22.332063993947404	27.23259265178624	28.48245114196315	21.952892212303198
46-47	22.3845352371638	27.22260741249431	28.228915607137438	22.16394174320445
48-49	22.33017568773731	27.31110403594632	28.171231191059125	22.18748908525724
50-51	22.309189872395898	27.45835209525366	28.078596018348573	22.153862014001874
52-53	22.49841560453489	27.422139449727585	27.91427437962934	22.165170566108184
54-55	22.474484480253	27.31415288721768	27.98676216561473	22.224600466914584
56-57	22.45472160285557	27.44346289862762	27.944853850821698	22.15696164769511
58-59	22.416014841818836	27.440393492465493	28.012383537893438	22.131208127822234
60-61	22.49714312480536	27.387094351151735	27.99849518213529	22.117267341907613
62-63	22.467422022857082	27.483616425405	27.976962245510457	22.071999306227458
64-65	22.47067738386342	27.399894667110157	27.939409814549432	22.19001813447699
66-67	22.527079929532135	27.436891257785724	27.716913756353645	22.3191150563285
68-69	22.44083634284922	27.440650007486106	27.99867538440114	22.119838265263535
70-71	22.530490091476324	27.228529809149215	28.006279800846386	22.23470029852808
72-73	22.496105817439833	27.105253081634384	28.021022759117827	22.377618341807956
74-75	22.355032951382437	27.323649082892377	28.11041792161087	22.21090004411432
76-77	22.48433294386979	27.341267240541573	27.860556985597384	22.313842829991255
78-79	22.470471736830415	27.288597990386066	27.798252258610777	22.442678014172746
80-81	22.42380024576421	27.354008211885027	27.855620268828662	22.366571273522105
82-83	22.551019303418276	27.320174439376427	27.85719216678213	22.27161409042317
84-85	22.580974177732095	27.330878792340453	27.770574015009963	22.31757301491749
86-87	22.385672786009028	27.38540943031656	27.85366059667384	22.375257187000575
88-89	22.527672826942492	27.37464779820551	27.741769335267346	22.355910039584654
90-91	22.544966619206512	27.391523020355535	27.779308099260767	22.28420226117718
92-93	22.57162674300191	27.346363324207783	27.785876314767272	22.296133618023035
94-95	22.444814698201988	27.422303767744005	27.771367740684582	22.36151379336943
96-97	22.597039962481055	27.533261402737182	27.765579033962617	22.10411960081915
98-99	23.63236790333805	29.459532510848636	27.978861653167336	18.929237932645982
100	22.578577225087322	30.28140075963054	21.90075790880072	25.239264106481425
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	866.0
1	786.0
2	918.0
3	1208.5
4	1675.0
5	2490.5
6	3126.5
7	3884.5
8	4874.0
9	5488.5
10	5609.0
11	5755.0
12	6485.0
13	8240.0
14	11651.0
15	15500.0
16	18359.5
17	20082.0
18	20176.5
19	18729.5
20	16671.5
21	15172.5
22	15108.5
23	16790.0
24	20642.5
25	27096.5
26	35635.0
27	45481.5
28	55635.5
29	66975.0
30	78529.5
31	90239.5
32	102850.0
33	115359.5
34	128718.0
35	141245.5
36	154314.5
37	166227.5
38	175087.0
39	182945.0
40	191438.0
41	198949.0
42	203115.5
43	207745.0
44	212089.0
45	215897.0
46	218089.5
47	217440.5
48	217238.5
49	216747.5
50	214691.5
51	208824.5
52	199742.5
53	188284.0
54	175320.5
55	163441.5
56	150669.5
57	135461.5
58	119693.0
59	104026.0
60	87420.5
61	70944.0
62	56145.0
63	43287.5
64	32341.5
65	23608.0
66	17245.5
67	13107.0
68	9959.5
69	7383.5
70	5326.5
71	3765.0
72	2618.0
73	1671.5
74	1027.5
75	655.0
76	418.5
77	239.5
78	139.5
79	87.5
80	54.0
81	38.5
82	30.5
83	23.0
84	15.0
85	7.5
86	6.5
87	6.5
88	5.0
89	3.5
90	2.0
91	1.5
92	1.0
93	1.5
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.011597478349283243
3	9.648484483596707E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.9563473997244455E-4
26-27	3.860226971695368E-4
28-29	0.0030792742256831934
30-31	0.013755334901351354
32-33	0.007855646497455526
34-35	6.689024164439093E-4
36-37	1.1668613704514528E-4
38-39	6.936711883281127E-4
40-41	9.821027487680995E-6
42-43	0.0015502744627632467
44-45	6.758632438434077E-4
46-47	0.0015725665859248879
48-49	0.0035531587884455314
50-51	0.002155308040793475
52-53	0.0
54-55	0.001741573027918446
56-57	0.04974040202827884
58-59	0.06951220303254833
60-61	0.04805215826105387
62-63	0.014653372299898318
64-65	0.0
66-67	0.0
68-69	2.615226327134738E-4
70-71	0.0017045260224489377
72-73	0.005759784364327336
74-75	0.010322133408088567
76-77	0.019657640721593577
78-79	0.014396809612231859
80-81	0.006833359145684913
82-83	0.038750835964918504
84-85	0.02760625677601446
86-87	0.0083270406053545
88-89	0.002552469118419108
90-91	0.0038761258025700168
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	29.0
20-21	147.0
22-23	371.0
24-25	403.0
26-27	958.0
28-29	2362.0
30-31	7407.0
32-33	9769.0
34-35	13577.0
36-37	22682.0
38-39	26824.0
40-41	26739.0
42-43	31142.0
44-45	37958.0
46-47	39098.0
48-49	36158.0
50-51	33352.0
52-53	32571.0
54-55	35502.0
56-57	36791.0
58-59	37194.0
60-61	36853.0
62-63	35869.0
64-65	38621.0
66-67	40823.0
68-69	41781.0
70-71	41875.0
72-73	39405.0
74-75	39829.0
76-77	42110.0
78-79	44045.0
80-81	42975.0
82-83	40576.0
84-85	40684.0
86-87	42379.0
88-89	43933.0
90-91	45550.0
92-93	43620.0
94-95	51330.0
96-97	161751.0
98-99	352266.0
100-101	3484852.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.65085212672462
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.46125567268858	80.1665699629138
2	6.2367923701083	10.933203315548939
3	1.3053987734106514	3.4325794456387406
4	0.4522761400945712	1.5856955630350018
5	0.21590039119916807	0.9461926631550139
6	0.1077757365170117	0.5667981086580856
7	0.0597162463921903	0.3663925917459447
8	0.03768047710020772	0.2642180741099792
9	0.02340031481116995	0.1845951779909395
>10	0.09721106383621453	1.3237683885514697
>50	0.0020259693535051586	0.11688312630040376
>100	5.338356430331598E-4	0.08767188472638139
>500	3.3008845402207806E-5	0.025431697625288678
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.9296968967193415E-5	0.0	0.0	0.0
3	0.0	1.9296968967193415E-5	0.0	3.859393793438683E-5	0.0
4	0.0	3.859393793438683E-5	0.0	3.859393793438683E-5	0.0
5	0.0	3.859393793438683E-5	0.0	3.859393793438683E-5	0.0
6	0.0	3.859393793438683E-5	0.0	3.859393793438683E-5	0.0
7	0.0	3.859393793438683E-5	0.0	3.859393793438683E-5	0.0
8	0.0	3.859393793438683E-5	0.0	3.859393793438683E-5	0.0
9	0.0	1.5437575173754732E-4	0.0	3.859393793438683E-5	0.0
10-11	9.648484483596707E-6	1.5437575173754732E-4	0.0	5.7890906901580244E-5	0.0
12-13	1.9296968967193415E-5	1.7367272070474075E-4	0.0	7.718787586877366E-5	0.0
14-15	1.9296968967193415E-5	3.0875150347509464E-4	0.0	9.648484483596709E-5	0.0
16-17	1.9296968967193415E-5	3.280484724422881E-4	0.0	1.3507878277035392E-4	0.0
18-19	1.9296968967193415E-5	3.376969569258848E-4	0.0	2.1226665863912758E-4	0.0
20-21	1.9296968967193415E-5	3.762908948602716E-4	0.0	2.31563627606321E-4	0.0
22-23	1.9296968967193415E-5	4.1488483279465845E-4	0.0	2.7015756554070783E-4	1.9296968967193415E-5
24-25	2.8945453450790122E-5	4.6312725521264195E-4	0.0	2.8945453450790123E-4	3.859393793438683E-5
26-27	3.859393793438683E-5	4.824242241798354E-4	0.0	3.1839998795869134E-4	3.859393793438683E-5
28-29	5.789090690158025E-5	4.824242241798354E-4	0.0	5.692605845322058E-4	3.859393793438683E-5
30-31	5.789090690158025E-5	5.403151310814157E-4	0.0	0.001418327219088716	3.859393793438683E-5
32-33	5.789090690158025E-5	5.403151310814157E-4	0.0	0.0028945453450790123	5.789090690158025E-5
34-35	6.753939138517696E-5	6.078545224665927E-4	0.0	0.004679514974544403	5.789090690158025E-5
36-37	8.683636035237036E-5	6.753939138517696E-4	0.0	0.007670545164459383	5.789090690158025E-5
38-39	9.648484483596707E-5	6.753939138517696E-4	0.0	0.012610569220060899	5.789090690158025E-5
40-41	9.648484483596707E-5	8.104726966221235E-4	0.0	0.0178786417481047	5.789090690158025E-5
42-43	1.157818138031605E-4	8.394181500729136E-4	0.0	0.023513356686525176	5.789090690158025E-5
44-45	1.2543029828675722E-4	8.876605724908971E-4	0.0	0.029572604942223912	5.789090690158025E-5
46-47	1.3507878277035392E-4	9.26254510425284E-4	0.0	0.036287950142807224	5.789090690158025E-5
48-49	1.3507878277035392E-4	9.26254510425284E-4	0.0	0.04294540443648895	5.789090690158025E-5
50-51	1.3507878277035392E-4	9.841454173268641E-4	0.0	0.048995004207704085	5.789090690158025E-5
52-53	1.5437575173754732E-4	9.841454173268641E-4	0.0	0.05532441002894352	6.753939138517696E-5
54-55	1.5437575173754732E-4	9.841454173268641E-4	0.0	0.062010809776076044	7.718787586877366E-5
56-57	1.5437575173754732E-4	0.0010227393552612511	0.0	0.06829197317489749	7.718787586877366E-5
58-59	1.5437575173754732E-4	0.0010420363242284445	0.0	0.07464067596510413	7.718787586877366E-5
60-61	1.5437575173754732E-4	0.0011095757156136215	0.0	0.0809025423949584	7.718787586877366E-5
62-63	1.5437575173754732E-4	0.0011192242000972181	0.0	0.08654690581786248	7.718787586877366E-5
64-65	1.5437575173754732E-4	0.0012735999518347656	0.0	0.09203689348902899	7.718787586877366E-5
66-67	1.5437575173754732E-4	0.001292896920801959	0.0	0.09815403265162931	7.718787586877366E-5
68-69	1.5437575173754732E-4	0.0013121938897691524	0.0	0.10347034760209109	8.683636035237036E-5
70-71	1.5437575173754732E-4	0.0013314908587363458	0.0	0.10820775348353708	1.0613332931956379E-4
72-73	1.5437575173754732E-4	0.0013507878277035392	0.0	0.1122408199976805	1.5437575173754732E-4
74-75	1.5437575173754732E-4	0.0013797332811543292	0.0	0.11507747443585795	1.5437575173754732E-4
76-77	1.5437575173754732E-4	0.0014569211570231028	0.0	0.11793342584300257	1.5437575173754732E-4
78-79	1.5437575173754732E-4	0.001485866610473893	0.0	0.11981488031730392	1.5437575173754732E-4
80-81	1.7367272070474075E-4	0.001485866610473893	0.0	0.12060605604495885	1.5437575173754732E-4
82-83	1.7367272070474075E-4	0.001485866610473893	0.0	0.12126215298984341	1.5437575173754732E-4
84-85	1.7367272070474075E-4	0.001485866610473893	0.0	0.12145512267951536	1.5437575173754732E-4
86-87	1.7367272070474075E-4	0.0015437575173754732	0.0	0.12159020146228572	1.5437575173754732E-4
88	1.7367272070474075E-4	0.0016402423622114404	0.0	0.12159020146228572	1.5437575173754732E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6825	0.0	50.709404	1
GTATCAA	12900	0.0	39.45807	1
TCAACGC	15315	0.0	32.443928	4
ATCAACG	15445	0.0	32.33826	3
TATCAAC	15735	0.0	31.96537	2
CAACGCA	15800	0.0	31.448025	5
AACGCAG	16270	0.0	30.592543	6
ACGCAGA	18630	0.0	26.71716	7
CGCAGAG	19160	0.0	25.978113	8
GTGGTAT	3485	0.0	24.239794	1
TGGTATC	3385	0.0	22.918673	2
GCAGAGT	22005	0.0	22.678185	9
GTACATG	16185	0.0	21.223705	1
GAGTACT	12775	0.0	20.779781	12-13
TACATGG	16215	0.0	20.466774	2
ACATGGG	16600	0.0	19.418455	3
CAGAGTA	21800	0.0	19.283768	10-11
GTACTTT	13865	0.0	19.0841	14-15
AGAGTAC	20205	0.0	18.38525	10-11
CATGGGG	11590	0.0	17.103916	4
>>END_MODULE