FastQCFastQC Report
Fri 27 May 2016
SRR2139458_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139458_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4953178
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63250.051.5633131
GTATCAA122700.042.0044671
ATCAACG147950.034.3268973
TCAACGC147100.034.3158264
CAACGCA150650.033.4487575
TATCAAC154050.033.284912
AACGCAG154700.032.658436
ACGCAGA178900.028.2406857
CGCAGAG183600.027.4218678
TGGTATC31150.025.2917062
GTGGTAT33550.025.0567071
GCAGAGT211500.023.8045169
GTACATG157150.021.2294121
GAGTACT122550.021.13376212-13
TACATGG159050.021.031152
ACATGGG160400.020.0016983
CAGAGTA208400.019.86116810-11
AGAGTAC194150.019.53950510-11
GTACTTT135500.019.08158514-15
AGTACTT134650.017.6821312-13
Produced by FastQC (version 0.11.5)