##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139458_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4953178
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.24111186797648	31.0	18.0	33.0	18.0	33.0
2	31.35075642345177	33.0	32.0	33.0	27.0	33.0
3	32.41534404780123	33.0	33.0	33.0	31.0	34.0
4	32.56044684846779	33.0	33.0	33.0	31.0	34.0
5	33.15525345545829	33.0	33.0	34.0	33.0	34.0
6	36.99119171570253	38.0	37.0	38.0	35.0	38.0
7	37.094547783261575	38.0	38.0	38.0	36.0	38.0
8	37.04429701496696	38.0	38.0	38.0	36.0	38.0
9	37.29447619286042	38.0	38.0	38.0	36.0	38.0
10-11	37.360767571849834	38.0	38.0	38.0	37.0	38.0
12-13	37.50052451173772	38.0	38.0	38.0	37.0	38.0
14-15	37.48599212465209	38.0	38.0	38.0	37.0	38.0
16-17	37.50415167797321	38.0	38.0	38.0	37.0	38.0
18-19	37.516547356061096	38.0	38.0	38.0	37.0	38.0
20-21	37.52502461845341	38.0	38.0	38.0	37.0	38.0
22-23	37.533207959840986	38.0	38.0	38.0	37.5	38.0
24-25	37.543036203505	38.0	38.0	38.0	38.0	38.0
26-27	37.51999344756119	38.0	38.0	38.0	38.0	38.0
28-29	37.49829061348541	38.0	38.0	38.0	37.0	38.0
30-31	37.49218817790156	38.0	38.0	38.0	37.0	38.0
32-33	37.40324953301082	38.0	38.0	38.0	37.0	38.0
34-35	37.45171165510659	38.0	38.0	38.0	37.0	38.0
36-37	37.44258100393252	38.0	38.0	38.0	37.0	38.0
38-39	37.428455148664355	38.0	38.0	38.0	37.0	38.0
40-41	37.410773483979	38.0	38.0	38.0	37.0	38.0
42-43	37.37253961610474	38.0	38.0	38.0	37.0	38.0
44-45	37.33232557811972	38.0	38.0	38.0	37.0	38.0
46-47	37.29728782034609	38.0	38.0	38.0	37.0	38.0
48-49	37.182010700841545	38.0	38.0	38.0	37.0	38.0
50-51	37.16514909741562	38.0	38.0	38.0	37.0	38.0
52-53	37.16726673850018	38.0	38.0	38.0	37.0	38.0
54-55	37.14314131508799	38.0	38.0	38.0	36.5	38.0
56-57	37.08515634444471	38.0	38.0	38.0	36.0	38.0
58-59	37.06264131988094	38.0	38.0	38.0	36.0	38.0
60-61	37.07504859034824	38.0	38.0	38.0	36.0	38.0
62-63	37.0733589444565	38.0	38.0	38.0	36.0	38.0
64-65	37.07291749946093	38.0	38.0	38.0	36.0	38.0
66-67	37.00883098511606	38.0	38.0	38.0	36.0	38.0
68-69	37.021150185632564	38.0	38.0	38.0	36.0	38.0
70-71	37.02938432831833	38.0	38.0	38.0	36.0	38.0
72-73	37.04090046668668	38.0	38.0	38.0	36.0	38.0
74-75	37.02302138580217	38.0	38.0	38.0	36.0	38.0
76-77	36.984614786932156	38.0	38.0	38.0	36.0	38.0
78-79	36.97844051948039	38.0	38.0	38.0	36.0	38.0
80-81	36.97386786184104	38.0	38.0	38.0	36.0	38.0
82-83	36.90793003320281	38.0	38.0	38.0	35.0	38.0
84-85	36.900246132272834	38.0	38.0	38.0	35.0	38.0
86-87	36.88797914114575	38.0	38.0	38.0	35.0	38.0
88-89	36.86091697489249	38.0	38.0	38.0	35.0	38.0
90-91	36.83134652189607	38.0	38.0	38.0	35.0	38.0
92-93	36.80996370795137	38.0	38.0	38.0	35.0	38.0
94-95	36.80275810686136	38.0	38.0	38.0	35.0	38.0
96-97	36.77343619216521	38.0	38.0	38.0	35.0	38.0
98-99	36.79752018166988	38.0	38.0	38.0	35.0	38.0
100	35.238325175231374	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	13.0
21	68.0
22	231.0
23	734.0
24	1762.0
25	3568.0
26	6652.0
27	11482.0
28	18427.0
29	27615.0
30	36787.0
31	47702.0
32	61362.0
33	82354.0
34	123605.0
35	227027.0
36	674749.0
37	3629037.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.04358858090705	19.131050812226007	14.517608694862167	25.30775191200478
2	14.414241971778944	20.04125035208175	43.261059405150945	22.283448270988362
3	18.621001959147023	25.72906757199825	29.82363235225237	25.82629811660236
4	12.687309036743683	17.976559695613602	38.49108592503641	30.845045342606305
5	13.941534101944248	36.99142247664025	35.2059627172696	13.861080704145904
6	30.29091625618946	37.339663545303644	18.7770558619133	13.592364336593596
7	27.233626572677178	32.235990711418	22.602781486956456	17.927601228948365
8	24.337445575345768	36.852319864135715	20.837995323406506	17.97223923711201
9	25.366199236126786	17.692560210838376	21.542593462217592	35.39864709081725
10-11	24.4922350862416	26.97920405848528	28.32181682144272	20.2067440338304
12-13	25.256542365325856	24.190297623061397	28.873785678608765	21.67937433300398
14-15	22.371414877478664	25.576024120271875	26.702371689448672	25.35018931280079
16-17	21.83289193321944	28.864528995323813	27.323649584165967	21.978929487290785
18-19	21.84430884575519	27.829395592082495	29.2371180684401	21.08917749372221
20-21	22.585149326021043	26.934184136792915	29.258778604686096	21.221887932499946
22-23	22.334832242868572	26.79268490151494	29.161947241776286	21.7105356138402
24-25	22.129287209236235	26.892438544452542	29.212067258480833	21.76620698783039
26-27	22.019123552315346	27.05367574690775	29.22495536343513	21.702245337341772
28-29	21.980588442194765	27.07572095029806	29.25433309389766	21.689357513609508
30-31	22.223322407483316	26.96199770382355	29.117196639939912	21.697483248753223
32-33	22.019871534409212	27.174796571203668	29.040761401022863	21.764570493364253
34-35	22.15199946704368	27.07665155206317	29.097623140347935	21.67372584054522
36-37	22.12680387838036	26.969261159736597	29.080610106648763	21.823324855234276
38-39	21.96358090726749	27.16232322415338	29.15776842128823	21.7163274472909
40-41	22.253936291503663	27.0412148041289	28.97448228208186	21.730366622285572
42-43	22.129795131978213	27.102376229732155	28.830073905587426	21.9377547327022
44-45	22.169518756365605	27.20442519759426	28.75418289166052	21.871873154379614
46-47	22.26655718314844	27.182160188460664	28.482665023379077	22.06861760501182
48-49	22.19487729119733	27.347502380295836	28.390420544944668	22.06719978356217
50-51	22.21832127858082	27.462737360132085	28.300300288994034	22.01864107229306
52-53	22.403443127142893	27.37266114177576	28.155382040216754	22.068513690864595
54-55	22.367015407746212	27.28237469130132	28.217830667887206	22.132779233065257
56-57	22.32677690182408	27.40232547495359	28.219409499570812	22.051488123651524
58-59	22.268691068204777	27.421844575471045	28.240296781583513	22.069167574740668
60-61	22.331189701977063	27.35504174039595	28.272895459708238	22.04087309791875
62-63	22.32194154944849	27.428038363412206	28.26749719428022	21.982522892859084
64-65	22.260197934277688	27.36255420989445	28.25767737242833	22.11957048339953
66-67	22.375221638741944	27.35149725942609	28.088993783892263	22.184287317939702
68-69	22.265464366719907	27.352099920034817	28.35888171175475	22.023554001490524
70-71	22.365700084798	27.166092067605057	28.36721593501497	22.10099191258198
72-73	22.32265479842524	27.052411325863062	28.344285805736536	22.280648069975165
74-75	22.20870237399358	27.312847650226246	28.379544172078386	22.09890580370178
76-77	22.359578420936693	27.347211147903106	28.127850971894087	22.165359459266114
78-79	22.30414538009802	27.261670897983425	28.072147940714103	22.36203578120445
80-81	22.276279870422975	27.324187899360812	28.142934829016614	22.256597401199592
82-83	22.40918770197834	27.35074279439856	28.07919869966663	22.16087080395647
84-85	22.419191063982126	27.358211202716802	28.022096301014056	22.200501432287012
86-87	22.30826527170603	27.381867520087706	28.100931450467257	22.208935757739003
88-89	22.392851532994218	27.36677903531401	27.987444394681148	22.252925037010623
90-91	22.427719139427566	27.42633670051657	27.98680799547802	22.15913616457785
92-93	22.429087846870978	27.43464016359199	27.9691470740123	22.16712491552473
94-95	22.30376866453678	27.492327053780073	27.97406733148355	22.229836950199598
96-97	22.4659725070136	27.5726774795302	27.96256474510997	21.99878526834623
98-99	23.49812868645541	29.49114578389038	28.209396180352837	18.801329349301373
100	22.426775200241092	30.368987832010795	22.144837104484964	25.05939986326315
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	989.0
1	861.5
2	999.0
3	1328.0
4	1679.5
5	2382.0
6	3037.5
7	4048.5
8	5501.0
9	6377.0
10	6398.5
11	6283.0
12	6835.0
13	8369.5
14	11411.0
15	14982.5
16	17823.5
17	19479.0
18	19165.5
19	17578.5
20	15662.5
21	13930.0
22	13503.5
23	15074.0
24	18811.5
25	24722.0
26	32430.0
27	41372.0
28	50960.5
29	62202.5
30	72973.0
31	83467.5
32	96212.5
33	108906.5
34	121273.5
35	132858.5
36	144801.5
37	155404.0
38	163706.5
39	171801.0
40	179845.5
41	186238.0
42	189304.5
43	192826.5
44	197101.5
45	200945.5
46	202272.5
47	201269.0
48	199786.5
49	197872.5
50	195773.0
51	190347.0
52	182624.0
53	172826.5
54	161333.0
55	149823.0
56	136805.0
57	122903.5
58	109086.5
59	94258.0
60	78507.0
61	63457.5
62	49875.0
63	38019.5
64	28003.5
65	20299.0
66	14769.5
67	11133.0
68	8623.5
69	6416.0
70	4588.5
71	3256.0
72	2202.0
73	1408.5
74	886.0
75	534.0
76	301.5
77	164.0
78	90.5
79	58.0
80	36.5
81	24.5
82	20.0
83	13.0
84	7.5
85	4.5
86	3.0
87	2.5
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00995320580039724
3	4.0378116837311316E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	4.6439808203592117E-4
26-27	5.048217545059885E-4
28-29	0.002958775870607177
30-31	0.012840207407233795
32-33	0.00782648896424609
34-35	6.884646731564587E-4
36-37	1.9272980569488173E-4
38-39	7.019861324165597E-4
40-41	2.0416048443199744E-5
42-43	0.0017723339691880253
44-45	7.615256652158231E-4
46-47	0.001634982330290645
48-49	0.003423346536629444
50-51	0.0020602414602991473
52-53	0.0
54-55	0.001636511738276373
56-57	0.046268894989669906
58-59	0.06504846078305794
60-61	0.04412411221695751
62-63	0.013735124600903929
64-65	0.0
66-67	0.0
68-69	1.9817849739081492E-4
70-71	0.001685728335562857
72-73	0.005853786489393754
74-75	0.010013236598717295
76-77	0.01781903006666607
78-79	0.012808296028171396
80-81	0.0054734550107348855
82-83	0.03518579739464741
84-85	0.02521173700081317
86-87	0.007642873900140832
88-89	0.002586088441126147
90-91	0.003080012714677488
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	30.0
20-21	141.0
22-23	301.0
24-25	330.0
26-27	710.0
28-29	1411.0
30-31	4251.0
32-33	5700.0
34-35	8092.0
36-37	13498.0
38-39	16543.0
40-41	16851.0
42-43	20498.0
44-45	26054.0
46-47	27179.0
48-49	24790.0
50-51	22903.0
52-53	22171.0
54-55	24826.0
56-57	26061.0
58-59	26635.0
60-61	27020.0
62-63	27190.0
64-65	29128.0
66-67	31350.0
68-69	32773.0
70-71	32856.0
72-73	31833.0
74-75	32631.0
76-77	34313.0
78-79	36778.0
80-81	36517.0
82-83	35434.0
84-85	34715.0
86-87	36497.0
88-89	39213.0
90-91	40189.0
92-93	40246.0
94-95	48028.0
96-97	160332.0
98-99	356529.0
100-101	3520631.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.17388055345118
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.56652807542885	80.73776109217098
2	6.198695712840331	10.931261107423467
3	1.3149078563098364	3.4782158478317426
4	0.4299430846015183	1.5163900074574652
5	0.2027547021930159	0.8938834446408775
6	0.09942172210708158	0.5259839429692938
7	0.05383414013621249	0.3322735529447707
8	0.03673157459194396	0.25910123764882
9	0.022733085873965463	0.18040179586161412
>10	0.07252736305480721	0.979595572669922
>50	0.001389076228289503	0.08152108076837065
>100	5.336066341244988E-4	0.08361131761274881
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.0189058418655658E-5	0.0
3	0.0	0.0	0.0	2.0189058418655658E-5	0.0
4	0.0	2.0189058418655658E-5	0.0	4.0378116837311316E-5	2.0189058418655658E-5
5	0.0	2.0189058418655658E-5	0.0	4.0378116837311316E-5	2.0189058418655658E-5
6	4.0378116837311316E-5	2.0189058418655658E-5	0.0	4.0378116837311316E-5	2.0189058418655658E-5
7	4.0378116837311316E-5	2.0189058418655658E-5	0.0	4.0378116837311316E-5	2.0189058418655658E-5
8	4.0378116837311316E-5	4.0378116837311316E-5	0.0	4.0378116837311316E-5	2.0189058418655658E-5
9	4.0378116837311316E-5	8.075623367462263E-5	0.0	4.0378116837311316E-5	2.0189058418655658E-5
10-11	4.0378116837311316E-5	8.075623367462263E-5	0.0	8.075623367462263E-5	2.0189058418655658E-5
12-13	4.0378116837311316E-5	8.075623367462263E-5	0.0	1.2113435051193395E-4	2.0189058418655658E-5
14-15	4.0378116837311316E-5	1.0094529209327829E-4	0.0	1.4132340893058961E-4	2.0189058418655658E-5
16-17	6.056717525596698E-5	1.0094529209327829E-4	0.0	1.8170152576790094E-4	2.0189058418655658E-5
18-19	8.075623367462263E-5	1.1103982130260612E-4	0.0	2.0189058418655658E-4	7.066170446529481E-5
20-21	8.075623367462263E-5	1.2113435051193395E-4	0.0	2.3217417181454008E-4	1.0094529209327829E-4
22-23	9.085076288395047E-5	1.6151246734924526E-4	0.0	2.725522886518514E-4	1.3122887972126176E-4
24-25	1.0094529209327829E-4	1.8170152576790094E-4	0.0	3.028358762798349E-4	1.8170152576790094E-4
26-27	1.0094529209327829E-4	1.8170152576790094E-4	0.0	3.432139931171462E-4	1.8170152576790094E-4
28-29	1.2113435051193395E-4	1.8170152576790094E-4	0.0	4.3406475600109664E-4	2.119851133958844E-4
30-31	1.2113435051193395E-4	1.8170152576790094E-4	0.0	7.167115738622759E-4	2.422687010238679E-4
32-33	1.2113435051193395E-4	1.8170152576790094E-4	0.0	0.0016555027903297642	2.422687010238679E-4
34-35	1.2113435051193395E-4	2.2207964260521226E-4	0.0	0.003018264233589021	2.422687010238679E-4
36-37	1.2113435051193395E-4	2.624577594425236E-4	0.0	0.005016981017035932	2.422687010238679E-4
38-39	1.2113435051193395E-4	3.028358762798349E-4	0.0	0.007813165608019739	2.8264681786117923E-4
40-41	1.2113435051193395E-4	3.432139931171462E-4	0.0	0.010992942308958007	3.028358762798349E-4
42-43	1.2113435051193395E-4	3.5330852232647405E-4	0.0	0.014425082240129467	3.028358762798349E-4
44-45	1.312288797212618E-4	3.734975807451297E-4	0.0	0.017927883875766226	3.028358762798349E-4
46-47	1.5141793813991744E-4	3.835921099544575E-4	0.0	0.021662859683217524	3.230249346984905E-4
48-49	1.716069965585731E-4	3.835921099544575E-4	0.0	0.025821805717460587	3.230249346984905E-4
50-51	2.5236323023319576E-4	5.047264604663915E-4	0.0	0.030212925923518194	3.432139931171462E-4
52-53	2.624577594425236E-4	5.047264604663915E-4	0.0	0.03474536953850639	3.5330852232647405E-4
54-55	2.624577594425236E-4	5.35010048094375E-4	0.0	0.038954788218796096	3.634030515358019E-4
56-57	2.624577594425236E-4	5.451045773037028E-4	0.0	0.04261910232178209	3.634030515358019E-4
58-59	2.624577594425236E-4	5.652936357223585E-4	0.0	0.046717481180769196	3.634030515358019E-4
60-61	2.624577594425236E-4	6.157662817689976E-4	0.0	0.05069472568924436	3.734975807451297E-4
62-63	2.8264681786117923E-4	6.46049869396981E-4	0.0	0.054621497551672886	4.239702267917688E-4
64-65	2.8264681786117923E-4	6.46049869396981E-4	0.0	0.05859874206014805	4.239702267917688E-4
66-67	3.028358762798349E-4	6.864279862342925E-4	0.0	0.06257598656862322	4.239702267917688E-4
68-69	3.230249346984905E-4	7.066170446529481E-4	0.0	0.06662389278156368	4.4415928521042447E-4
70-71	3.230249346984905E-4	7.369006322809316E-4	0.0	0.07015697800482842	4.6434834362908017E-4
72-73	3.634030515358019E-4	7.469951614902594E-4	0.0	0.0728421227745096	4.845374020477358E-4
74-75	3.634030515358019E-4	7.570896906995872E-4	0.0	0.07507301372977107	4.845374020477358E-4
76-77	3.835921099544575E-4	7.67184219908915E-4	0.0	0.07764711867814966	5.047264604663915E-4
78-79	3.835921099544575E-4	7.67184219908915E-4	0.0	0.07913101447192086	5.047264604663915E-4
80-81	3.835921099544575E-4	7.67184219908915E-4	0.0	0.07949441752345665	5.148209896757193E-4
82-83	3.835921099544575E-4	7.67184219908915E-4	0.0	0.07989819869182976	5.249155188850472E-4
84-85	3.835921099544575E-4	7.67184219908915E-4	0.0	0.08013037286364431	5.249155188850472E-4
86-87	3.835921099544575E-4	7.67184219908915E-4	0.0	0.08023131815573759	5.249155188850472E-4
88	3.835921099544575E-4	7.67184219908915E-4	0.0	0.08025150721415625	5.249155188850472E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6325	0.0	51.563313	1
GTATCAA	12270	0.0	42.004467	1
ATCAACG	14795	0.0	34.326897	3
TCAACGC	14710	0.0	34.315826	4
CAACGCA	15065	0.0	33.448757	5
TATCAAC	15405	0.0	33.28491	2
AACGCAG	15470	0.0	32.65843	6
ACGCAGA	17890	0.0	28.240685	7
CGCAGAG	18360	0.0	27.421867	8
TGGTATC	3115	0.0	25.291706	2
GTGGTAT	3355	0.0	25.056707	1
GCAGAGT	21150	0.0	23.804516	9
GTACATG	15715	0.0	21.229412	1
GAGTACT	12255	0.0	21.133762	12-13
TACATGG	15905	0.0	21.03115	2
ACATGGG	16040	0.0	20.001698	3
CAGAGTA	20840	0.0	19.861168	10-11
AGAGTAC	19415	0.0	19.539505	10-11
GTACTTT	13550	0.0	19.081585	14-15
AGTACTT	13465	0.0	17.68213	12-13
>>END_MODULE