FastQCFastQC Report
Fri 27 May 2016
SRR2139457_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139457_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5059846
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74600.049.623771
GTATCAA137800.036.6362461
TCAACGC167800.030.2345094
ATCAACG169850.030.0683923
TATCAAC172400.029.6997342
CAACGCA171050.029.6353685
AACGCAG176750.028.7274176
GTGGTAT36800.027.0703071
TGGTATC35200.026.9816722
ACGCAGA203300.024.9549947
CTTATAC51850.024.7487811
CGCAGAG210050.024.1731558
GCAGAGT235900.021.506369
TATACAC68800.020.9197433
GAGTACT131450.019.8113912-13
TTATACA72350.018.786522
CAGAGTA235000.018.66113510-11
GTACTTT143600.017.97375514-15
AGAGTAC217050.017.83199310-11
TATGCCG39700.017.7134642-43
Produced by FastQC (version 0.11.5)