##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139457_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5059846
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.806402803563586	18.0	18.0	32.0	18.0	33.0
2	30.10510734911695	32.0	27.0	33.0	25.0	33.0
3	31.267087970661557	33.0	31.0	33.0	29.0	33.0
4	32.10720978464562	33.0	32.0	33.0	31.0	33.0
5	32.58134457056598	33.0	33.0	33.0	32.0	34.0
6	37.06113763146151	38.0	37.0	38.0	36.0	38.0
7	37.00857377872765	38.0	38.0	38.0	35.0	38.0
8	37.35314948320561	38.0	38.0	38.0	37.0	38.0
9	37.481787983270635	38.0	38.0	38.0	37.0	38.0
10-11	37.42634350926886	38.0	38.0	38.0	37.0	38.0
12-13	37.545893491620106	38.0	38.0	38.0	37.5	38.0
14-15	37.5529556630775	38.0	38.0	38.0	38.0	38.0
16-17	37.584960293257936	38.0	38.0	38.0	38.0	38.0
18-19	37.578720775296325	38.0	38.0	38.0	38.0	38.0
20-21	37.58390147836012	38.0	38.0	38.0	38.0	38.0
22-23	37.59138030128466	38.0	38.0	38.0	38.0	38.0
24-25	37.59902986806517	38.0	38.0	38.0	38.0	38.0
26-27	37.56638281671128	38.0	38.0	38.0	38.0	38.0
28-29	37.5410583788949	38.0	38.0	38.0	38.0	38.0
30-31	37.53903121971401	38.0	38.0	38.0	38.0	38.0
32-33	37.45233508774028	38.0	38.0	38.0	37.5	38.0
34-35	37.49376046765057	38.0	38.0	38.0	37.0	38.0
36-37	37.48037326629988	38.0	38.0	38.0	37.5	38.0
38-39	37.45946592333918	38.0	38.0	38.0	37.5	38.0
40-41	37.46856440041155	38.0	38.0	38.0	38.0	38.0
42-43	37.43511257709545	38.0	38.0	38.0	37.0	38.0
44-45	37.39674236225458	38.0	38.0	38.0	37.0	38.0
46-47	37.36764676494238	38.0	38.0	38.0	37.0	38.0
48-49	37.251962061008555	38.0	38.0	38.0	37.0	38.0
50-51	37.243933810539595	38.0	38.0	38.0	37.0	38.0
52-53	37.25215766545005	38.0	38.0	38.0	37.0	38.0
54-55	37.23773380278777	38.0	38.0	38.0	37.0	38.0
56-57	37.18834145989989	38.0	38.0	38.0	37.0	38.0
58-59	37.174513745213105	38.0	38.0	38.0	37.0	38.0
60-61	37.18588743683456	38.0	38.0	38.0	37.0	38.0
62-63	37.19246301997956	38.0	38.0	38.0	37.0	38.0
64-65	37.20082669879062	38.0	38.0	38.0	37.0	38.0
66-67	37.13550556960078	38.0	38.0	38.0	36.5	38.0
68-69	37.15635887093541	38.0	38.0	38.0	36.0	38.0
70-71	37.174265208659136	38.0	38.0	38.0	36.0	38.0
72-73	37.19069473930791	38.0	38.0	38.0	36.0	38.0
74-75	37.180301021791166	38.0	38.0	38.0	36.0	38.0
76-77	37.150845462185025	38.0	38.0	38.0	36.0	38.0
78-79	37.14954125635525	38.0	38.0	38.0	36.0	38.0
80-81	37.148627165229605	38.0	38.0	38.0	36.0	38.0
82-83	37.093642751081866	38.0	38.0	38.0	36.0	38.0
84-85	37.09398178077283	38.0	38.0	38.0	36.0	38.0
86-87	37.0890461822479	38.0	38.0	38.0	36.0	38.0
88-89	37.072048447942294	38.0	38.0	38.0	36.0	38.0
90-91	37.050490147485675	38.0	38.0	38.0	36.0	38.0
92-93	37.03641128053685	38.0	38.0	38.0	36.0	38.0
94-95	37.029157195162696	38.0	38.0	38.0	36.0	38.0
96-97	37.003155942380644	38.0	38.0	38.0	36.0	38.0
98-99	37.02545170992974	38.0	38.0	38.0	36.0	38.0
100	35.67038943205037	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	7.0
20	7.0
21	61.0
22	205.0
23	606.0
24	1493.0
25	3060.0
26	5786.0
27	10190.0
28	16863.0
29	24873.0
30	33529.0
31	42743.0
32	54773.0
33	72638.0
34	110544.0
35	219144.0
36	814539.0
37	3648785.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.76496517878212	22.05114543011783	14.418976387818919	23.764913003281126
2	14.499240620817188	20.270502644979757	41.61854498046802	23.611711753735033
3	18.357925010306648	25.427098316508694	30.63941522284688	25.575561450337776
4	13.12496467283787	17.813941372919253	37.94969254004964	31.11140141419324
5	13.789411772611263	36.85214925513544	35.123677677146695	14.23476129510661
6	30.433870912276777	37.36890411289197	18.279232213786745	13.917992761044506
7	26.916649241893925	32.427311028833685	22.488767444700887	18.167272284571506
8	24.35750020850437	36.92582738684142	20.669561879946542	18.04711052470767
9	25.399290808455433	17.37473828254852	21.297150150419597	35.92882075857645
10-11	24.614484314344747	26.7508932090028	28.078028461735794	20.55659401491666
12-13	25.39925128156074	24.109498589482765	28.613934099970635	21.877316028985863
14-15	22.515695141709845	25.48428351376702	26.19927563012787	25.800745714395262
16-17	22.079002009152056	28.776063540273753	26.87700376651779	22.26793068405639
18-19	22.10482295310964	27.650139944970658	28.80924636836773	21.435790733551972
20-21	22.84910523742346	26.680706149558343	28.782102693043054	21.688085919975144
22-23	22.646933696211274	26.696926259782856	28.67876160984593	21.977378434159938
24-25	22.418894332627247	26.877807241587504	28.74778457264996	21.95551385313529
26-27	22.317693457995286	26.991775383122402	28.77903250022956	21.91149865865275
28-29	22.21696309646075	26.84820505224777	28.809200883915448	22.12563096737603
30-31	22.590084360830364	26.829287847309175	28.7413577906826	21.839270001177866
32-33	22.368670330178265	27.013322864268023	28.68911061811988	21.92889618743383
34-35	22.433775822475997	26.980548699268113	28.78454136609074	21.801134112165148
36-37	22.437118301998147	26.84759885633049	28.67747207369152	22.037810767979852
38-39	22.14376382547415	26.845847156713663	28.915901920918035	22.09448709689415
40-41	22.392514580018847	26.702901370368455	28.822083037367896	22.082501012244798
42-43	22.339718761774012	26.852522111578697	28.624668824414183	22.183090302233104
44-45	22.283772460664743	26.977819923369285	28.549997091871976	22.188410524093996
46-47	22.536618799585444	26.914554422378167	28.14011452450005	22.40871225353634
48-49	22.453936794952682	27.053600487125745	28.040526632959068	22.451936084962508
50-51	22.302898665008648	27.172434321753062	28.104002352571584	22.420664660666706
52-53	22.500113207084794	27.148721079585382	28.0756127433353	22.27555296999453
54-55	22.443537961977995	27.095356557525808	27.97612719505388	22.484978285442324
56-57	22.503039815297097	27.29470305142856	27.831816531221914	22.370440602052426
58-59	22.310595861201314	27.410634157295167	28.053676189963934	22.225093791539592
60-61	22.387862597432655	27.537915868139308	27.86133530801413	22.212886226413907
62-63	22.267427891440185	27.771540380147737	27.83726315362874	22.12376857478334
64-65	22.253806716382183	27.688392647822063	27.79891402686454	22.258886608931217
66-67	22.269576913490315	27.774544632824817	27.6581627699542	22.297715683730672
68-69	22.19820817879125	27.533849983574072	27.89104069875125	22.376901138883426
70-71	22.383639562287392	27.31226941037027	27.832889423060724	22.471201604281614
72-73	22.518045196183685	27.29571842777328	27.809993138690785	22.37624323735225
74-75	22.37484880758962	27.413859093367186	27.71581019584935	22.49548190319384
76-77	22.579462371284592	27.170949766548276	27.701473298955197	22.54811456321194
78-79	22.539968155823104	27.239514742222738	27.615666344680996	22.60485075727317
80-81	22.551575425608625	27.279570246100278	27.588490221420052	22.580364106871045
82-83	22.651967573631982	27.213514130688388	27.6488276964471	22.48569059923253
84-85	22.6623270201056	27.252975387728632	27.600242412295767	22.48445517987
86-87	22.58178843883979	27.290187161320123	27.634314112461517	22.49371028737857
88-89	22.632826829113604	27.26976522795893	27.56290515906547	22.534502783861992
90-91	22.699640297562823	27.279681933126408	27.528048518992044	22.492629250318725
92-93	22.772840200216187	27.29303764618881	27.502967946181244	22.43115420741376
94-95	22.632965291415	27.31473191916926	27.562182979460488	22.49011980995525
96-97	22.750078990987607	27.396341024127935	27.60027403329467	22.253305951589788
98-99	23.825384847768312	29.261908870604124	27.796211818087794	19.116494463539766
100	30.439328728969073	40.366074224511095	29.19459704651983	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1220.0
1	1141.0
2	1193.0
3	1390.0
4	1775.5
5	2487.5
6	3143.0
7	4140.5
8	5289.5
9	6037.0
10	6262.0
11	6257.5
12	6902.5
13	8631.0
14	12328.0
15	16735.5
16	19829.0
17	21372.0
18	21044.5
19	19207.0
20	17137.0
21	15585.0
22	15234.0
23	16983.0
24	20933.0
25	27063.5
26	35249.5
27	45044.0
28	55674.0
29	67590.5
30	78641.5
31	89158.5
32	101754.5
33	114571.5
34	125836.0
35	136128.0
36	148689.5
37	159857.5
38	169116.5
39	177518.0
40	184842.0
41	191976.5
42	196899.0
43	201686.0
44	210613.0
45	220230.0
46	220557.5
47	218411.5
48	217513.5
49	214511.5
50	211120.0
51	206816.0
52	200555.5
53	192052.0
54	181569.0
55	169333.0
56	155905.0
57	141338.0
58	126143.5
59	109151.0
60	91368.5
61	74184.0
62	58841.5
63	45552.5
64	34286.0
65	25605.5
66	19025.5
67	14412.5
68	10968.5
69	8114.0
70	5903.5
71	4249.0
72	2923.5
73	1901.5
74	1237.0
75	801.0
76	497.5
77	316.0
78	216.0
79	152.5
80	107.0
81	73.0
82	48.0
83	29.0
84	15.5
85	7.0
86	5.5
87	4.5
88	3.5
89	3.5
90	3.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.008893551305711676
3	7.905378938410378E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.767269018772856E-4
26-27	3.261804271975171E-4
28-29	0.002274105443545975
30-31	0.011064762709334819
32-33	0.006288185352693804
34-35	5.172027605896271E-4
36-37	6.991372646154644E-5
38-39	5.025135728916037E-4
40-41	0.0
42-43	0.0012535011407879488
44-45	5.651806970034428E-4
46-47	0.001224268953213732
48-49	0.0026186617779393666
50-51	0.001712392478664858
52-53	0.0
54-55	0.001256402417318251
56-57	0.04613926130620346
58-59	0.06566040547540046
60-61	0.043614736421425764
62-63	0.012100967264712723
64-65	0.0
66-67	0.0
68-69	1.2630896274620489E-4
70-71	0.0012548542817893804
72-73	0.005198938804843952
74-75	0.00851916479391582
76-77	0.01789782323576799
78-79	0.01280254041234131
80-81	0.0056322508327203095
82-83	0.036895452381170965
84-85	0.024997376779793797
86-87	0.0076528747251015175
88-89	0.002370278501317747
90-91	0.003590931924616357
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	42.0
20-21	162.0
22-23	422.0
24-25	480.0
26-27	1172.0
28-29	3171.0
30-31	10165.0
32-33	13172.0
34-35	18226.0
36-37	29459.0
38-39	34304.0
40-41	33854.0
42-43	38379.0
44-45	45781.0
46-47	46333.0
48-49	44240.0
50-51	39002.0
52-53	35951.0
54-55	38495.0
56-57	39890.0
58-59	42165.0
60-61	43075.0
62-63	42380.0
64-65	44586.0
66-67	47897.0
68-69	49799.0
70-71	55262.0
72-73	43360.0
74-75	42376.0
76-77	44353.0
78-79	45783.0
80-81	45101.0
82-83	42194.0
84-85	41863.0
86-87	43303.0
88-89	45159.0
90-91	45493.0
92-93	44267.0
94-95	50573.0
96-97	153791.0
98-99	1153764.0
100-101	2420602.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.57557236374714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.89446837028557	81.39605132960301
2	5.963669303896258	10.564708439614412
3	1.2685875117440324	3.370975948386884
4	0.41692716347179626	1.477182485540317
5	0.17936032820367812	0.7943471864995163
6	0.09585055807330871	0.5094010825636741
7	0.051597011081436814	0.31991643521578095
8	0.033874068770440566	0.24003320237045642
9	0.02147055748453623	0.17115902263453783
>10	0.0720864779300832	0.9838162531977769
>50	0.0015190903755463083	0.08883631937180067
>100	5.89558683331993E-4	0.08357229500178914
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.9763447346025945E-5	0.0	0.0	1.9763447346025945E-5
5	0.0	9.881723673012973E-5	0.0	0.0	1.9763447346025945E-5
6	5.9290342038077836E-5	9.881723673012973E-5	0.0	0.0	3.952689469205189E-5
7	7.905378938410378E-5	9.881723673012973E-5	0.0	0.0	3.952689469205189E-5
8	7.905378938410378E-5	9.881723673012973E-5	0.0	0.0	3.952689469205189E-5
9	7.905378938410378E-5	2.3716136815231135E-4	0.0	0.0	3.952689469205189E-5
10-11	7.905378938410378E-5	2.3716136815231135E-4	0.0	1.9763447346025945E-5	3.952689469205189E-5
12-13	7.905378938410378E-5	2.3716136815231135E-4	0.0	1.9763447346025945E-5	3.952689469205189E-5
14-15	9.881723673012973E-5	3.2609688120942813E-4	0.0	1.9763447346025945E-5	4.940861836506487E-5
16-17	1.383441314221816E-4	3.5574205222846703E-4	0.0	2.9645171019038918E-5	5.9290342038077836E-5
18-19	1.383441314221816E-4	3.75505499574493E-4	0.0	3.952689469205189E-5	5.9290342038077836E-5
20-21	1.383441314221816E-4	4.3479584161257084E-4	0.0	3.952689469205189E-5	5.9290342038077836E-5
22-23	1.482258550951946E-4	4.446775652855838E-4	0.0	3.952689469205189E-5	6.91720657110908E-5
24-25	1.5810757876820756E-4	4.6444101263160974E-4	0.0	3.952689469205189E-5	7.905378938410378E-5
26-27	1.5810757876820756E-4	4.743227363046227E-4	0.0	1.4822585509519461E-4	9.881723673012973E-5
28-29	1.7787102611423352E-4	4.743227363046227E-4	0.0	5.731399730347525E-4	9.881723673012973E-5
30-31	1.9763447346025947E-4	5.336130783427005E-4	0.0	0.001531667169317011	9.881723673012973E-5
32-33	1.9763447346025947E-4	5.336130783427005E-4	0.0	0.004239259455722565	9.881723673012973E-5
34-35	1.9763447346025947E-4	5.731399730347525E-4	0.0	0.007836206872699288	9.881723673012973E-5
36-37	2.3716136815231135E-4	6.126668677268044E-4	0.0	0.013666423839776943	9.881723673012973E-5
38-39	2.3716136815231135E-4	6.324303150728303E-4	0.0	0.02928942896681045	9.881723673012973E-5
40-41	2.668065391713503E-4	6.521937624188563E-4	0.0	0.04528793959341845	9.881723673012973E-5
42-43	2.766882628443632E-4	6.521937624188563E-4	0.0	0.05570327634477413	9.881723673012973E-5
44-45	3.063334338634021E-4	7.213658281299471E-4	0.0	0.06691903271364386	1.0869896040314271E-4
46-47	3.359786048824411E-4	8.004196175140509E-4	0.0	0.07839171389801192	1.1858068407615567E-4
48-49	3.359786048824411E-4	8.103013411870638E-4	0.0	0.09077351366029718	1.1858068407615567E-4
50-51	3.359786048824411E-4	9.091185779171935E-4	0.0	0.10326401238298558	1.1858068407615567E-4
52-53	3.359786048824411E-4	9.190003015902065E-4	0.0	0.14002402444659384	1.1858068407615567E-4
54-55	3.4586032855545403E-4	9.684089199552713E-4	0.0	0.18661635156485	1.1858068407615567E-4
56-57	3.5574205222846703E-4	0.0010079358146473233	0.0	0.2478533931665114	1.1858068407615567E-4
58-59	3.5574205222846703E-4	0.0010178175383203363	0.0	0.2861944810178017	1.1858068407615567E-4
60-61	3.5574205222846703E-4	0.0010276992619933493	0.0	0.3013728085795496	1.1858068407615567E-4
62-63	3.5574205222846703E-4	0.001047462709339375	0.0	0.31415975901242843	1.1858068407615567E-4
64-65	3.5574205222846703E-4	0.0011858068407615567	0.0	0.32176868624064847	1.1858068407615567E-4
66-67	3.5574205222846703E-4	0.0012055702881075827	0.0	0.3282016883517799	1.1858068407615567E-4
68-69	3.5574205222846703E-4	0.0012450971827996347	0.0	0.3346742173576034	1.1858068407615567E-4
70-71	3.5574205222846703E-4	0.0012450971827996347	0.0	0.3403166815748938	1.1858068407615567E-4
72-73	3.5574205222846703E-4	0.0012450971827996347	0.0	0.3440618548469657	1.383441314221816E-4
74-75	3.5574205222846703E-4	0.0012450971827996347	0.0	0.347332705382733	1.383441314221816E-4
76-77	3.5574205222846703E-4	0.0012945058011646993	0.0	0.350396039721367	1.383441314221816E-4
78-79	3.5574205222846703E-4	0.0013241509721837383	0.0	0.3521154596404713	1.383441314221816E-4
80-81	3.5574205222846703E-4	0.0013340326958567513	0.0	0.3527676534028901	1.383441314221816E-4
82-83	3.6562377590148003E-4	0.0013439144195297643	0.0	0.35332102992857883	1.383441314221816E-4
84-85	3.75505499574493E-4	0.0013537961432027773	0.0	0.35357795474407716	1.383441314221816E-4
86-87	3.75505499574493E-4	0.0013834413142218163	0.0	0.3536866537044803	1.482258550951946E-4
88	3.75505499574493E-4	0.0014032047615678421	0.0	0.3536866537044803	1.5810757876820756E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7460	0.0	49.62377	1
GTATCAA	13780	0.0	36.636246	1
TCAACGC	16780	0.0	30.234509	4
ATCAACG	16985	0.0	30.068392	3
TATCAAC	17240	0.0	29.699734	2
CAACGCA	17105	0.0	29.635368	5
AACGCAG	17675	0.0	28.727417	6
GTGGTAT	3680	0.0	27.070307	1
TGGTATC	3520	0.0	26.981672	2
ACGCAGA	20330	0.0	24.954994	7
CTTATAC	5185	0.0	24.748781	1
CGCAGAG	21005	0.0	24.173155	8
GCAGAGT	23590	0.0	21.50636	9
TATACAC	6880	0.0	20.919743	3
GAGTACT	13145	0.0	19.81139	12-13
TTATACA	7235	0.0	18.78652	2
CAGAGTA	23500	0.0	18.661135	10-11
GTACTTT	14360	0.0	17.973755	14-15
AGAGTAC	21705	0.0	17.831993	10-11
TATGCCG	3970	0.0	17.71346	42-43
>>END_MODULE