FastQCFastQC Report
Fri 27 May 2016
SRR2139456_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139456_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3904214
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51350.047.4584471
GTATCAA94500.036.329791
TCAACGC112800.030.319824
ATCAACG114300.030.034133
TATCAAC116600.029.8106272
CAACGCA114850.029.7414075
AACGCAG119650.028.5840026
ACGCAGA138300.024.6366637
CGCAGAG141250.024.1221288
GTGGTAT26600.022.5022281
TGGTATC25900.020.964432
GCAGAGT167450.020.2457459
GAGTACT80500.019.19809512-13
CAGAGTA164250.017.9853310-11
AGAGTAC151800.017.2355610-11
TACATGG137550.017.2198072
GTACTTT90000.017.00570314-15
GTACATG137850.016.9652271
ACATGGG139000.016.5160123
TCCGGCG3854.7769072E-415.47051194
Produced by FastQC (version 0.11.5)