##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139456_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3904214
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.726659194398668	18.0	18.0	32.0	18.0	33.0
2	29.85925054313109	32.0	27.0	33.0	25.0	33.0
3	31.058275237986443	33.0	31.0	33.0	28.0	33.0
4	32.16973736583087	33.0	32.0	33.0	31.0	33.0
5	32.53247004390641	33.0	33.0	33.0	32.0	34.0
6	37.01890623823387	38.0	37.0	38.0	35.0	38.0
7	37.00808920822475	38.0	38.0	38.0	35.0	38.0
8	37.348945267856735	38.0	38.0	38.0	37.0	38.0
9	37.45826868096882	38.0	38.0	38.0	37.0	38.0
10-11	37.464106347654095	38.0	38.0	38.0	37.0	38.0
12-13	37.53304698461713	38.0	38.0	38.0	37.5	38.0
14-15	37.54123313937197	38.0	38.0	38.0	37.5	38.0
16-17	37.570602046916484	38.0	38.0	38.0	38.0	38.0
18-19	37.57203114378464	38.0	38.0	38.0	38.0	38.0
20-21	37.574606939245356	38.0	38.0	38.0	38.0	38.0
22-23	37.58092071605682	38.0	38.0	38.0	38.0	38.0
24-25	37.5879315626985	38.0	38.0	38.0	38.0	38.0
26-27	37.55974177969435	38.0	38.0	38.0	38.0	38.0
28-29	37.53891528748397	38.0	38.0	38.0	38.0	38.0
30-31	37.533819432564094	38.0	38.0	38.0	38.0	38.0
32-33	37.434343780669415	38.0	38.0	38.0	37.0	38.0
34-35	37.49245320433463	38.0	38.0	38.0	37.0	38.0
36-37	37.486652978976764	38.0	38.0	38.0	37.5	38.0
38-39	37.474214009437524	38.0	38.0	38.0	37.5	38.0
40-41	37.46320873399346	38.0	38.0	38.0	37.5	38.0
42-43	37.42966224766878	38.0	38.0	38.0	37.0	38.0
44-45	37.396945485079144	38.0	38.0	38.0	37.0	38.0
46-47	37.371064871290386	38.0	38.0	38.0	37.0	38.0
48-49	37.24689556931921	38.0	38.0	38.0	37.0	38.0
50-51	37.24256221824068	38.0	38.0	38.0	37.0	38.0
52-53	37.2530137149526	38.0	38.0	38.0	37.0	38.0
54-55	37.22986702762905	38.0	38.0	38.0	37.0	38.0
56-57	37.16916204914895	38.0	38.0	38.0	36.5	38.0
58-59	37.14729578183257	38.0	38.0	38.0	36.0	38.0
60-61	37.155445350707495	38.0	38.0	38.0	36.0	38.0
62-63	37.15228310750027	38.0	38.0	38.0	36.0	38.0
64-65	37.15638371784855	38.0	38.0	38.0	36.0	38.0
66-67	37.069145133039825	38.0	38.0	38.0	36.0	38.0
68-69	37.09063971513211	38.0	38.0	38.0	36.0	38.0
70-71	37.10665869463304	38.0	38.0	38.0	36.0	38.0
72-73	37.11687211394639	38.0	38.0	38.0	36.0	38.0
74-75	37.10059998039483	38.0	38.0	38.0	36.0	38.0
76-77	37.06471738006983	38.0	38.0	38.0	36.0	38.0
78-79	37.06170312330673	38.0	38.0	38.0	36.0	38.0
80-81	37.0571364339489	38.0	38.0	38.0	36.0	38.0
82-83	36.98875493165646	38.0	38.0	38.0	36.0	38.0
84-85	36.985095610238844	38.0	38.0	38.0	36.0	38.0
86-87	36.97637242354217	38.0	38.0	38.0	36.0	38.0
88-89	36.950350922869745	38.0	38.0	38.0	35.5	38.0
90-91	36.92397575899364	38.0	38.0	38.0	35.0	38.0
92-93	36.90202269443643	38.0	38.0	38.0	35.0	38.0
94-95	36.8922970011231	38.0	38.0	38.0	35.0	38.0
96-97	36.86513353777546	38.0	38.0	38.0	35.0	38.0
98-99	36.87948797389507	38.0	38.0	38.0	35.0	38.0
100	35.44549865647542	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	7.0
21	44.0
22	182.0
23	526.0
24	1192.0
25	2624.0
26	4657.0
27	8148.0
28	13238.0
29	19435.0
30	25464.0
31	32319.0
32	42330.0
33	57833.0
34	90795.0
35	177849.0
36	659682.0
37	2767886.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.9436900743658	23.37095251438574	14.514112187497918	23.171245223750542
2	14.281030248196902	21.058415363760158	41.80137177893744	22.859182609105506
3	18.2473138632549	26.262066132150064	30.789559893376715	24.701060111218318
4	12.94644709536926	18.50275112993294	39.053059079241045	29.497742695456758
5	13.594772212793663	37.75146546782528	35.17394282178179	13.47981949759926
6	29.658671374058905	38.89937897871377	18.492531403247874	12.949418243979455
7	26.595314703548524	33.462356315509346	22.73174574959262	17.21058323134951
8	24.139148110221416	37.91362358723164	20.72937087977247	17.21785742277447
9	25.595471969518062	18.086662257755336	21.517903475577928	34.799962297148674
10-11	24.33871452743113	27.734878262308367	28.303417794209025	19.622989416051475
12-13	25.137159489720595	25.058821058476816	28.747937484984178	21.056081966818418
14-15	22.23510289138864	26.579421619818994	26.49840659349103	24.687068895301334
16-17	21.70817224670574	29.701074275129386	27.157527737977478	21.433225740187396
18-19	21.676296432521372	28.785294556087344	28.9770617081953	20.56134730319598
20-21	22.632245241130768	27.829896719074476	28.83769417601621	20.70016386377855
22-23	22.349491740893583	27.72172434501501	28.710181332381822	21.21860258170959
24-25	22.031635063198397	27.94357999579873	28.808363092340876	21.216421848661998
26-27	21.943378414681185	28.1095746930904	28.70869359744595	21.238353294782463
28-29	21.857107504562013	28.172169595209656	28.75391819097896	21.216804709249374
30-31	22.174723698078893	27.996845709148417	28.697401545792378	21.131029046980306
32-33	21.93632749502478	28.186645595357867	28.556618774680086	21.32040813493726
34-35	22.082309475475146	28.22259650052223	28.64754394574216	21.047550078260468
36-37	22.074295483118995	28.045473582726256	28.616860924225396	21.263370009929357
38-39	21.90809917905616	28.226353765404156	28.681877402373534	21.18366965316615
40-41	22.156921071996873	28.123395617351722	28.539954370915797	21.17972893973561
42-43	22.110162354936268	28.166993498188848	28.35837665876273	21.364467488112155
44-45	22.080361218398004	28.32204481596038	28.267824360766053	21.32976960487557
46-47	22.196856534927225	28.312136555425642	27.997337562246933	21.4936693474002
48-49	22.16369725833677	28.427767620069112	27.972614961454262	21.435920160139855
50-51	22.10298308390582	28.537472389818596	27.87722389069894	21.48232063557664
52-53	22.25671945071781	28.457223688102996	27.81893810069854	21.46711876048066
54-55	22.28986487018501	28.40733358593997	27.796615835452243	21.506185708422784
56-57	22.27683120809533	28.555833962499598	27.713551983757412	21.453782845647655
58-59	22.205521140685587	28.643771688741026	27.76727621011062	21.383430960462768
60-61	22.302467096903534	28.720653875465953	27.687983462549276	21.28889556508124
62-63	22.253392802672867	28.828186549432438	27.661303830362293	21.257116817532403
64-65	22.244132844809375	28.870698901885227	27.59166690753492	21.293501345770476
66-67	22.418880082844023	29.21116160748149	27.15173639589348	21.21822191378101
68-69	22.26237904770994	28.708035011430326	27.633358929223316	21.39622701163642
70-71	22.341420688654186	28.398460657781026	27.6736478617956	21.586470791769187
72-73	22.341551232028213	28.615066374761668	27.60207883076439	21.44130356244573
74-75	22.147461118956134	28.633939222008703	27.624009321271576	21.594590337763584
76-77	22.214947756091526	28.19093097188658	27.84898715008712	21.745134121934782
78-79	22.158660054168127	28.245114154017507	27.776761247008995	21.819464544805367
80-81	22.172058951233733	28.294607301914176	27.735690183478273	21.797643563373818
82-83	22.21357361656742	28.159872200527573	27.91946464382275	21.707089539082254
84-85	22.294990196339036	28.1060010556815	27.866210090745575	21.732798657233886
86-87	22.155630654556756	28.201668241509232	27.848578687947146	21.794122415986873
88-89	22.2106020170094	28.1350506705504	27.857682827789848	21.796664484650353
90-91	22.24873801608852	28.133520165799673	27.91170012214379	21.706041695968015
92-93	22.263645274819105	28.106819676263417	27.87070332747043	21.758831721447045
94-95	22.10199563347262	28.206192047461588	27.9128269369396	21.778985382126198
96-97	22.20096747685087	28.324316539448823	27.959149094790703	21.5155668889096
98-99	23.219395065833307	30.20782856195474	28.179682677443573	18.393093694768375
100	29.35552693636565	41.27234994755607	29.372123116078274	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1543.0
1	1421.5
2	1033.5
3	806.0
4	1005.5
5	1384.5
6	1692.0
7	2032.0
8	2487.5
9	2879.5
10	3134.5
11	3314.5
12	3782.5
13	4998.0
14	7544.5
15	10393.0
16	12552.0
17	13960.5
18	14080.5
19	13184.0
20	12323.5
21	11867.0
22	12301.0
23	14294.5
24	17878.5
25	23369.0
26	30751.5
27	39301.5
28	48086.0
29	57869.0
30	67811.0
31	77251.5
32	87954.5
33	99714.0
34	110834.0
35	120913.5
36	131768.5
37	141323.0
38	148189.5
39	154154.5
40	159552.5
41	163688.5
42	166002.0
43	168789.5
44	171672.0
45	172616.5
46	171425.5
47	168873.5
48	166261.0
49	162207.5
50	156595.5
51	149892.5
52	140992.5
53	130575.0
54	119900.5
55	109491.0
56	98460.0
57	86766.5
58	75375.5
59	63919.0
60	52730.0
61	42019.5
62	32426.0
63	24677.0
64	18578.5
65	13791.0
66	9875.0
67	7213.0
68	5346.0
69	3950.0
70	2921.5
71	1981.5
72	1290.0
73	849.5
74	570.0
75	362.5
76	221.5
77	130.0
78	75.5
79	48.0
80	32.0
81	23.5
82	18.0
83	9.0
84	3.5
85	2.5
86	2.5
87	3.0
88	1.5
89	0.0
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.009681846333218416
3	2.56133500878794E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.665135382551405E-4
26-27	2.1778175193889812E-4
28-29	0.002447708112217293
30-31	0.01305778750798154
32-33	0.007839433998005955
34-35	5.53984929548577E-4
36-37	3.879158522588857E-5
38-39	7.542155774384993E-4
40-41	1.3086983947505492E-5
42-43	0.001580674255044689
44-45	4.778279216887819E-4
46-47	0.0015261125896256472
48-49	0.003213279105423097
50-51	0.0020691042711484917
52-53	0.0
54-55	0.0014780446822576898
56-57	0.052881874226051014
58-59	0.07561024965134806
60-61	0.05065927097912909
62-63	0.014849911050316664
64-65	0.0
66-67	0.0
68-69	1.756481930631259E-4
70-71	0.0016553699981037145
72-73	0.005804535476007721
74-75	0.010539225945044863
76-77	0.020516979244111933
78-79	0.01550139650699819
80-81	0.0071947086639177925
82-83	0.04266936676247433
84-85	0.029705949067109823
86-87	0.009040882198423085
88-89	0.002662892782737483
90-91	0.0040299626910296135
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	34.0
20-21	144.0
22-23	352.0
24-25	484.0
26-27	1107.0
28-29	2410.0
30-31	7015.0
32-33	9005.0
34-35	12256.0
36-37	20244.0
38-39	24646.0
40-41	24264.0
42-43	27396.0
44-45	31634.0
46-47	32066.0
48-49	30692.0
50-51	28472.0
52-53	25761.0
54-55	27303.0
56-57	28577.0
58-59	29447.0
60-61	28783.0
62-63	27948.0
64-65	29212.0
66-67	31012.0
68-69	32626.0
70-71	33019.0
72-73	29870.0
74-75	30615.0
76-77	31732.0
78-79	33134.0
80-81	32998.0
82-83	31381.0
84-85	30627.0
86-87	31665.0
88-89	33097.0
90-91	34132.0
92-93	33095.0
94-95	38045.0
96-97	117453.0
98-99	892179.0
100-101	1958282.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.4139689355535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.2916629350684	82.52163882686781
2	5.767130116186618	10.31323986112011
3	1.1948354671379937	3.2050494402532235
4	0.35405631005606164	1.2663031963515758
5	0.16278058851568764	0.7277429242426405
6	0.07727633647890023	0.41457503696266595
7	0.04345448279680494	0.2719806442432842
8	0.028810739310182486	0.20608660397527898
9	0.020950333226771305	0.16859271998952216
>10	0.0572197519484243	0.7568813601940578
>50	0.001430144596467873	0.08525182568731489
>100	3.927946776767122E-4	0.0626575601124771
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.5613350087879405E-5	0.0
4	0.0	7.684005026363821E-5	0.0	5.122670017575881E-5	0.0
5	0.0	7.684005026363821E-5	0.0	5.122670017575881E-5	0.0
6	0.0	7.684005026363821E-5	0.0	5.122670017575881E-5	5.122670017575881E-5
7	0.0	7.684005026363821E-5	0.0	5.122670017575881E-5	5.122670017575881E-5
8	0.0	7.684005026363821E-5	0.0	5.122670017575881E-5	7.684005026363821E-5
9	0.0	1.5368010052727642E-4	0.0	5.122670017575881E-5	7.684005026363821E-5
10-11	0.0	1.5368010052727642E-4	0.0	5.122670017575881E-5	7.684005026363821E-5
12-13	0.0	1.5368010052727642E-4	0.0	7.684005026363821E-5	7.684005026363821E-5
14-15	0.0	1.5368010052727642E-4	0.0	7.684005026363821E-5	8.964672530757792E-5
16-17	0.0	1.5368010052727642E-4	0.0	7.684005026363821E-5	1.0245340035151762E-4
18-19	0.0	1.5368010052727642E-4	0.0	7.684005026363821E-5	1.5368010052727642E-4
20-21	0.0	1.5368010052727642E-4	0.0	7.684005026363821E-5	2.0490680070303524E-4
22-23	0.0	1.5368010052727642E-4	0.0	7.684005026363821E-5	2.0490680070303524E-4
24-25	0.0	1.7929345061515583E-4	0.0	7.684005026363821E-5	2.0490680070303524E-4
26-27	0.0	1.7929345061515583E-4	0.0	1.2806675043939701E-4	2.0490680070303524E-4
28-29	0.0	1.7929345061515583E-4	0.0	3.2016687609849256E-4	2.0490680070303524E-4
30-31	0.0	1.7929345061515583E-4	0.0	0.0016008343804924628	2.0490680070303524E-4
32-33	0.0	2.0490680070303524E-4	0.0	0.0037779691379622123	2.1771347574697493E-4
34-35	0.0	2.0490680070303524E-4	0.0	0.006723504398068344	2.5613350087879403E-4
36-37	0.0	2.0490680070303524E-4	0.0	0.011782141040424526	2.5613350087879403E-4
38-39	0.0	2.0490680070303524E-4	0.0	0.023794802231639968	2.5613350087879403E-4
40-41	0.0	2.8174685096667347E-4	0.0	0.036793577401238765	3.0736020105455285E-4
42-43	0.0	3.0736020105455285E-4	0.0	0.04542527638085413	3.0736020105455285E-4
44-45	0.0	3.3297355114243223E-4	0.0	0.05465888908753465	3.0736020105455285E-4
46-47	0.0	3.3297355114243223E-4	0.0	0.0653524627492243	3.0736020105455285E-4
48-49	0.0	3.5858690123031166E-4	0.0	0.07517518250792606	3.0736020105455285E-4
50-51	0.0	5.122670017575881E-4	0.0	0.08568946271900055	3.0736020105455285E-4
52-53	0.0	5.378803518454675E-4	0.0	0.11191753320898906	3.2016687609849256E-4
54-55	0.0	5.378803518454675E-4	0.0	0.14407509424432163	3.4578022618637195E-4
56-57	0.0	5.378803518454675E-4	0.0	0.180740604895121	3.5858690123031166E-4
58-59	0.0	5.378803518454675E-4	0.0	0.20895371001692017	3.5858690123031166E-4
60-61	0.0	6.01913727065166E-4	0.0	0.21981377045418105	3.5858690123031166E-4
62-63	0.0	6.147204021091057E-4	0.0	0.23209537182131923	3.5858690123031166E-4
64-65	0.0	6.915604523727439E-4	0.0	0.23878045619425575	3.5858690123031166E-4
66-67	0.0	6.915604523727439E-4	0.0	0.24504292029074226	3.5858690123031166E-4
68-69	0.0	6.915604523727439E-4	0.0	0.25104925088635	3.5858690123031166E-4
70-71	0.0	6.915604523727439E-4	0.0	0.2560566608285304	3.5858690123031166E-4
72-73	0.0	6.915604523727439E-4	0.0	0.2602060235427669	3.8420025131819104E-4
74-75	0.0	7.171738024606233E-4	0.0	0.2637534725299382	3.8420025131819104E-4
76-77	0.0	7.299804775045631E-4	0.0	0.2668142678654397	3.8420025131819104E-4
78-79	0.0	7.427871525485028E-4	0.0	0.2686456223967231	3.8420025131819104E-4
80-81	0.0	7.427871525485028E-4	0.0	0.26945244292449133	4.098136014060704E-4
82-83	0.0	7.427871525485028E-4	0.0	0.26993909657616105	4.3542695149394986E-4
84-85	0.0	7.427871525485028E-4	0.0	0.2700927766766883	4.3542695149394986E-4
86-87	0.0	7.427871525485028E-4	0.0	0.2701952300770398	4.482336265378896E-4
88	0.0	8.19627202812141E-4	0.0	0.2701952300770398	4.610403015818293E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5135	0.0	47.458447	1
GTATCAA	9450	0.0	36.32979	1
TCAACGC	11280	0.0	30.31982	4
ATCAACG	11430	0.0	30.03413	3
TATCAAC	11660	0.0	29.810627	2
CAACGCA	11485	0.0	29.741407	5
AACGCAG	11965	0.0	28.584002	6
ACGCAGA	13830	0.0	24.636663	7
CGCAGAG	14125	0.0	24.122128	8
GTGGTAT	2660	0.0	22.502228	1
TGGTATC	2590	0.0	20.96443	2
GCAGAGT	16745	0.0	20.245745	9
GAGTACT	8050	0.0	19.198095	12-13
CAGAGTA	16425	0.0	17.98533	10-11
AGAGTAC	15180	0.0	17.23556	10-11
TACATGG	13755	0.0	17.219807	2
GTACTTT	9000	0.0	17.005703	14-15
GTACATG	13785	0.0	16.965227	1
ACATGGG	13900	0.0	16.516012	3
TCCGGCG	385	4.7769072E-4	15.470511	94
>>END_MODULE