FastQCFastQC Report
Fri 27 May 2016
SRR2139455_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139455_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5615875
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72900.047.5964661
GTATCAA132400.036.1229061
TCAACGC158150.030.2933924
ATCAACG160400.030.0278913
CAACGCA161800.029.6363565
TATCAAC168400.029.185362
AACGCAG170150.028.2571186
ACGCAGA196050.024.4588727
GTGGTAT36700.024.2749351
CGCAGAG201750.023.7467168
TGGTATC36000.023.0892562
GCAGAGT234950.020.3367219
GAGTACT129150.019.22437912-13
CAGAGTA228050.018.26060910-11
TACATGG177900.017.9945852
GTACATG180450.017.9765191
AGAGTAC211700.017.93938810-11
GTACTTT138650.017.8303114-15
ACATGGG179850.017.253283
AGTACTT138100.016.4815612-13
Produced by FastQC (version 0.11.5)