##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139455_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5615875
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.43627680459412	18.0	18.0	32.0	18.0	33.0
2	29.705568767111092	32.0	27.0	33.0	25.0	33.0
3	31.03779963051172	33.0	30.0	33.0	28.0	33.0
4	32.13369154406036	33.0	32.0	33.0	31.0	33.0
5	32.56805822779175	33.0	33.0	33.0	32.0	34.0
6	37.0556515235827	38.0	37.0	38.0	36.0	38.0
7	37.04679965277005	38.0	38.0	38.0	35.0	38.0
8	37.35299414605916	38.0	38.0	38.0	37.0	38.0
9	37.47046898301689	38.0	38.0	38.0	37.0	38.0
10-11	37.438835889331585	38.0	38.0	38.0	37.0	38.0
12-13	37.54345262314421	38.0	38.0	38.0	37.5	38.0
14-15	37.55430008680749	38.0	38.0	38.0	38.0	38.0
16-17	37.581962383421995	38.0	38.0	38.0	38.0	38.0
18-19	37.580815456184474	38.0	38.0	38.0	38.0	38.0
20-21	37.58271025099894	38.0	38.0	38.0	38.0	38.0
22-23	37.58778417939207	38.0	38.0	38.0	38.0	38.0
24-25	37.59627630049802	38.0	38.0	38.0	38.0	38.0
26-27	37.56838305593577	38.0	38.0	38.0	38.0	38.0
28-29	37.5469204547484	38.0	38.0	38.0	38.0	38.0
30-31	37.54302554902088	38.0	38.0	38.0	38.0	38.0
32-33	37.456424856315564	38.0	38.0	38.0	38.0	38.0
34-35	37.5047422441457	38.0	38.0	38.0	38.0	38.0
36-37	37.49379926761608	38.0	38.0	38.0	38.0	38.0
38-39	37.48416270326302	38.0	38.0	38.0	38.0	38.0
40-41	37.47119431863286	38.0	38.0	38.0	38.0	38.0
42-43	37.43540738798332	38.0	38.0	38.0	37.0	38.0
44-45	37.40225185472681	38.0	38.0	38.0	37.0	38.0
46-47	37.37670153396759	38.0	38.0	38.0	37.0	38.0
48-49	37.26707884407463	38.0	38.0	38.0	37.0	38.0
50-51	37.25683731193361	38.0	38.0	38.0	37.0	38.0
52-53	37.26269900109297	38.0	38.0	38.0	37.0	38.0
54-55	37.244002134065816	38.0	38.0	38.0	37.0	38.0
56-57	37.19097480135132	38.0	38.0	38.0	37.0	38.0
58-59	37.17261960822502	38.0	38.0	38.0	37.0	38.0
60-61	37.18288355840769	38.0	38.0	38.0	37.0	38.0
62-63	37.185534972392915	38.0	38.0	38.0	36.5	38.0
64-65	37.18812786714231	38.0	38.0	38.0	36.0	38.0
66-67	37.12132507173527	38.0	38.0	38.0	36.0	38.0
68-69	37.13763305113953	38.0	38.0	38.0	36.0	38.0
70-71	37.15191243031843	38.0	38.0	38.0	36.0	38.0
72-73	37.162810055957905	38.0	38.0	38.0	36.0	38.0
74-75	37.14768969382935	38.0	38.0	38.0	36.0	38.0
76-77	37.114249031339384	38.0	38.0	38.0	36.0	38.0
78-79	37.111942091261355	38.0	38.0	38.0	36.0	38.0
80-81	37.10748770275021	38.0	38.0	38.0	36.0	38.0
82-83	37.04992670748756	38.0	38.0	38.0	36.0	38.0
84-85	37.042735993847224	38.0	38.0	38.0	36.0	38.0
86-87	37.03358014162181	38.0	38.0	38.0	36.0	38.0
88-89	37.00902837184093	38.0	38.0	38.0	36.0	38.0
90-91	36.98380538171379	38.0	38.0	38.0	36.0	38.0
92-93	36.9654792289582	38.0	38.0	38.0	36.0	38.0
94-95	36.95852445785702	38.0	38.0	38.0	35.0	38.0
96-97	36.93295385386985	38.0	38.0	38.0	35.0	38.0
98-99	36.950910403052184	38.0	38.0	38.0	35.0	38.0
100	35.560124468046254	38.0	36.0	38.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	1.0
19	4.0
20	23.0
21	65.0
22	236.0
23	707.0
24	1767.0
25	3412.0
26	6483.0
27	11440.0
28	18658.0
29	27703.0
30	36410.0
31	46829.0
32	59829.0
33	81642.0
34	126714.0
35	247634.0
36	923498.0
37	4022818.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.72644957375297	23.34487501947604	14.51317915729962	23.415496249471364
2	14.287490011858441	20.610662553698226	41.796474664623716	23.305372769819616
3	18.22637212982926	25.786948696087585	30.58113189270118	25.405547281381967
4	12.891455027043872	18.103198522046878	38.83827542457765	30.167071026331605
5	13.681554521779777	37.268974113561995	35.20760344558951	13.841867919068712
6	30.031900638814076	38.15248736839762	18.611739043336968	13.20387294945133
7	26.877610345671865	32.70181405390968	22.83480312506956	17.5857724753489
8	24.185296147083047	37.518320831571216	20.703309813697775	17.593073207647965
9	25.399372315088918	17.74352171300109	21.49638302134574	35.36072295056425
10-11	24.36373672847063	27.257533331849444	28.329173993367018	20.049555946312907
12-13	25.221590580274665	24.546228325950985	28.793553987579852	21.438627106194495
14-15	22.29519887818016	25.972453090569147	26.570819329133926	25.16152870211677
16-17	21.757321877712734	29.19413270416453	27.208965655396533	21.8395797627262
18-19	21.704168985242728	28.259078059963944	29.074979411044584	20.96177354374875
20-21	22.602396821901873	27.335292504030807	28.987671683780697	21.074638990286623
22-23	22.372480233634874	27.21905050217252	28.824239618206427	21.58422964598618
24-25	22.063440629560482	27.354896939185387	28.930953025254595	21.650709405999528
26-27	21.995479573925795	27.56491878639562	28.822258022286878	21.617343617391708
28-29	21.929028495201802	27.577049506585787	28.874065235458264	21.619856762754146
30-31	22.16839803561189	27.41576129848658	28.83832857574728	21.57751209015425
32-33	21.951416304426395	27.602963160993372	28.7444165080595	21.70120402652073
34-35	22.106661528778044	27.60106731216319	28.778097044648128	21.51417411441064
36-37	22.13744837132811	27.529399176458902	28.682545541355314	21.65060691085768
38-39	21.98370802004922	27.632382512577795	28.812321861303808	21.57158760606918
40-41	22.23040460933047	27.49970408651964	28.667560780628865	21.602330523521022
42-43	22.102872549618073	27.616565284826965	28.469809732839924	21.810752432715034
44-45	22.165238465199845	27.706289446962696	28.363073380067977	21.765398707769485
46-47	22.208313931562948	27.73912540282455	28.104240552195897	21.948320113416607
48-49	22.18246163136405	27.83650789293894	28.07783821872219	21.903192256974823
50-51	22.22389580741001	27.93888378650787	27.95132982562938	21.88589058045274
52-53	22.35597467587096	27.893843996036527	27.842194266220876	21.90798706187164
54-55	22.319187580416987	27.825607511306146	27.89655879075379	21.958646117523077
56-57	22.286721203125378	27.940508647259747	27.851917891788585	21.920852257826283
58-59	22.261335668191574	27.94555000896139	27.902476575515134	21.890637747331905
60-61	22.309774582217038	27.966713051608753	27.902512815605483	21.82099955056872
62-63	22.35117242763337	28.028653590628068	27.85051682061055	21.769657161128016
64-65	22.27420199930574	28.00350722997375	27.84257687670874	21.87971389401177
66-67	22.36236311224606	28.14816652029874	27.62703259491348	21.862437772541714
68-69	22.29404258753554	27.906114538669087	27.888338232033927	21.911504641761447
70-71	22.341431066480276	27.756569879237876	27.831495095827446	22.070503958454406
72-73	22.36823599137965	27.913228885657688	27.81202791402009	21.90650720894258
74-75	22.234759304134876	27.986863762060217	27.748674379545758	22.02970255425915
76-77	22.309556029912255	27.712453871202786	27.886868153852333	22.09112194503262
78-79	22.21255258334629	27.734119840128635	27.83496035983478	22.21836721669029
80-81	22.252403946778777	27.820667122475246	27.80841199384453	22.118516936901447
82-83	22.36440538390492	27.724834926464037	27.912080562071335	21.998679127559708
84-85	22.405295369167046	27.665874201769817	27.8730052383011	22.055825190762036
86-87	22.2327186814744	27.724926620095065	27.909473683272413	22.132881015158127
88-89	22.343949279696652	27.69273239237539	27.82534684971453	22.137971478213426
90-91	22.35383321975898	27.73932808729791	27.860225047866187	22.04661364507692
92-93	22.402114824889352	27.7417578550343	27.808277405847925	22.047849914228422
94-95	22.275063439306997	27.76404322175628	27.855140927010435	22.10575241192629
96-97	22.386487423179044	27.902646909655783	27.88302136551041	21.82784430165477
98-99	23.428350078275624	29.77113588500914	28.148353790026555	18.652160246688677
100	29.716728141650968	40.90084303435081	29.38242882399823	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1177.0
1	1147.5
2	1147.5
3	1259.5
4	1662.5
5	2350.0
6	2851.0
7	3446.5
8	4293.5
9	4905.5
10	5203.0
11	5546.5
12	6410.5
13	8287.5
14	11989.5
15	16219.5
16	19421.0
17	21329.5
18	21283.0
19	19715.0
20	18102.5
21	17038.0
22	17435.5
23	20266.5
24	25402.5
25	32832.5
26	42264.5
27	53032.5
28	64742.0
29	77871.0
30	90937.0
31	103984.0
32	118963.5
33	134723.5
34	148556.5
35	162304.0
36	177679.5
37	190771.0
38	201646.0
39	210838.5
40	219460.5
41	227260.5
42	233006.0
43	238461.5
44	242879.0
45	245568.5
46	246775.5
47	246686.5
48	244521.0
49	239917.5
50	233859.0
51	225850.0
52	214238.5
53	200364.0
54	185049.0
55	169140.5
56	152475.0
57	135187.5
58	118407.0
59	99934.5
60	82123.5
61	66315.0
62	51940.0
63	39685.5
64	29461.5
65	21838.5
66	16232.5
67	12343.0
68	9322.0
69	6835.0
70	5022.5
71	3584.5
72	2564.5
73	1795.0
74	1166.0
75	738.5
76	478.5
77	295.0
78	172.5
79	109.0
80	75.0
81	50.5
82	40.5
83	33.5
84	22.5
85	14.0
86	10.5
87	11.5
88	8.0
89	3.0
90	1.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.008297905491130056
3	1.246466490083914E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.849423209958948E-4
26-27	2.849748269267727E-4
28-29	0.003037728228263273
30-31	0.012518865234415751
32-33	0.007469265446883203
34-35	5.64304666477132E-4
36-37	5.394960082240772E-5
38-39	7.597632288345341E-4
40-41	9.105029335949268E-6
42-43	0.0016039722785929369
44-45	6.096348351995395E-4
46-47	0.0014909283069418835
48-49	0.0027635708719630095
50-51	0.0017156850199464971
52-53	0.0
54-55	0.0015400935934127887
56-57	0.04851494200043051
58-59	0.06827537918967225
60-61	0.046097046028245936
62-63	0.012949199623010079
64-65	0.0
66-67	0.0
68-69	2.451898351650036E-4
70-71	0.0017016449234259785
72-73	0.005923252963083873
74-75	0.009254010184873275
76-77	0.018787866643044
78-79	0.013041067800061801
80-81	0.006240748387950878
82-83	0.03740671400262
84-85	0.025548312454537574
86-87	0.008020235946430171
88-89	0.0027345919808709103
90-91	0.0035379339898874247
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	38.0
20-21	150.0
22-23	415.0
24-25	529.0
26-27	1373.0
28-29	3348.0
30-31	10738.0
32-33	13146.0
34-35	18462.0
36-37	30351.0
38-39	37072.0
40-41	35744.0
42-43	39900.0
44-45	46866.0
46-47	47071.0
48-49	45167.0
50-51	41568.0
52-53	39162.0
54-55	42052.0
56-57	43102.0
58-59	43227.0
60-61	42636.0
62-63	40973.0
64-65	42839.0
66-67	44448.0
68-69	45735.0
70-71	45958.0
72-73	42984.0
74-75	43860.0
76-77	45290.0
78-79	47065.0
80-81	46699.0
82-83	44335.0
84-85	43781.0
86-87	44981.0
88-89	47412.0
90-91	48117.0
92-93	46989.0
94-95	53839.0
96-97	168983.0
98-99	1296464.0
100-101	2793006.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.66551304441326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.05000200879921	79.8194513879624
2	6.595573533157531	11.564086752128167
3	1.3744368453140654	3.6147213357480723
4	0.4825339061830373	1.6920632978744294
5	0.20613493690027412	0.9035462499870142
6	0.10492242422575537	0.5518846889768652
7	0.051926520543235904	0.3186515544823828
8	0.033777017125626765	0.23688636283424025
9	0.02594324000567024	0.20468947006182855
>10	0.07300062148760604	0.9635375240927115
>50	0.0013226197245792544	0.07844958317356605
>100	4.2632653342330096E-4	0.05203179267827544
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.903332072027957E-5	0.0	1.780666414405591E-5	0.0
3	0.0	8.903332072027957E-5	0.0	1.780666414405591E-5	0.0
4	0.0	1.424533131524473E-4	0.0	3.561332828811182E-5	0.0
5	0.0	1.6025997729650322E-4	0.0	3.561332828811182E-5	0.0
6	3.561332828811182E-5	1.6025997729650322E-4	0.0	3.561332828811182E-5	0.0
7	3.561332828811182E-5	1.6025997729650322E-4	0.0	3.561332828811182E-5	0.0
8	3.561332828811182E-5	1.7806664144055914E-4	0.0	3.561332828811182E-5	0.0
9	3.561332828811182E-5	2.492932980167828E-4	0.0	3.561332828811182E-5	0.0
10-11	6.23233245041957E-5	2.492932980167828E-4	0.0	8.903332072027957E-5	0.0
12-13	7.122665657622365E-5	2.492932980167828E-4	0.0	1.1574331693636343E-4	1.780666414405591E-5
14-15	7.122665657622365E-5	2.760032942328666E-4	0.0	1.246466490083914E-4	1.780666414405591E-5
16-17	1.9587330558461504E-4	2.9380995837692255E-4	0.0	1.246466490083914E-4	1.780666414405591E-5
18-19	1.9587330558461504E-4	3.1161662252097847E-4	0.0	1.246466490083914E-4	1.780666414405591E-5
20-21	1.9587330558461504E-4	3.472299508090903E-4	0.0	1.7806664144055914E-4	4.4516660360139785E-5
22-23	1.9587330558461504E-4	4.1845660738531397E-4	0.0	2.492932980167828E-4	5.341999243216774E-5
24-25	1.9587330558461504E-4	5.074899281055935E-4	0.0	2.5819663008881075E-4	5.341999243216774E-5
26-27	1.9587330558461504E-4	5.520065884657333E-4	0.0	3.027132904489505E-4	5.341999243216774E-5
28-29	1.9587330558461504E-4	5.609099205377613E-4	0.0	4.718765998174817E-4	5.341999243216774E-5
30-31	1.9587330558461504E-4	6.410399091860129E-4	0.0	0.00121975649386783	5.341999243216774E-5
32-33	2.04776637656643E-4	6.588465733300688E-4	0.0	0.003107262893137757	5.341999243216774E-5
34-35	2.225833018006989E-4	7.389765619783203E-4	0.0	0.005297482582856634	5.341999243216774E-5
36-37	2.3148663387272686E-4	8.19106550626572E-4	0.0	0.008698555434371313	5.341999243216774E-5
38-39	2.3148663387272686E-4	8.369132147706279E-4	0.0	0.014120684666236338	5.341999243216774E-5
40-41	2.3148663387272686E-4	9.437531996349634E-4	0.0	0.01977430053197409	5.341999243216774E-5
42-43	2.3148663387272686E-4	9.793665279230752E-4	0.0	0.02575733968437688	5.341999243216774E-5
44-45	2.3148663387272686E-4	0.0011040131769314665	0.0	0.03182050882542792	5.341999243216774E-5
46-47	2.3148663387272686E-4	0.0011752398335076902	0.0	0.038827431166113924	5.341999243216774E-5
48-49	2.3148663387272686E-4	0.001210853161795802	0.0	0.04553164021635096	5.341999243216774E-5
50-51	2.3148663387272686E-4	0.001255369822155942	0.0	0.05227146259487613	5.341999243216774E-5
52-53	2.3148663387272686E-4	0.0012642731542279698	0.0	0.05838805172835934	5.341999243216774E-5
54-55	2.3148663387272686E-4	0.0012909831504440538	0.0	0.06440670420905023	5.341999243216774E-5
56-57	2.3148663387272686E-4	0.0013176931466601376	0.0	0.07033632336902085	5.341999243216774E-5
58-59	2.3148663387272686E-4	0.0013265964787321657	0.0	0.07659536581565651	5.341999243216774E-5
60-61	2.6709996216083866E-4	0.0013444031428762214	0.0	0.08255169497184321	5.341999243216774E-5
62-63	2.849066263048946E-4	0.0013889198032363613	0.0	0.08824982749794111	5.341999243216774E-5
64-65	3.027132904489505E-4	0.0014957597881006966	0.0	0.09440202995971242	5.341999243216774E-5
66-67	3.2051995459300643E-4	0.0015758897767489482	0.0	0.1001624858103145	5.341999243216774E-5
68-69	3.2051995459300643E-4	0.00161150310503706	0.0	0.10571816502325995	5.341999243216774E-5
70-71	3.3832661873706236E-4	0.0016471164333251719	0.0	0.11062390099494736	5.341999243216774E-5
72-73	3.828432790972021E-4	0.0016560197653972	0.0	0.11459478709907182	5.341999243216774E-5
74-75	3.917466111692301E-4	0.0016560197653972	0.0	0.11784450330536203	5.341999243216774E-5
76-77	4.09553275313286E-4	0.0017450530861174793	0.0	0.120702472900483	5.341999243216774E-5
78-79	4.2735993945734193E-4	0.0017806664144055912	0.0	0.12287488592605783	5.341999243216774E-5
80-81	4.2735993945734193E-4	0.0017806664144055912	0.0	0.12376521913326062	5.341999243216774E-5
82-83	4.2735993945734193E-4	0.0017984730785496471	0.0	0.12428161239343824	5.341999243216774E-5
84-85	4.2735993945734193E-4	0.001816279742693703	0.0	0.12453980902352706	5.341999243216774E-5
86-87	4.3626327152936984E-4	0.001834086406837759	0.0	0.12466445567253545	5.341999243216774E-5
88	4.629732677454537E-4	0.0018696997351258708	0.0	0.1246822623366795	5.341999243216774E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7290	0.0	47.596466	1
GTATCAA	13240	0.0	36.122906	1
TCAACGC	15815	0.0	30.293392	4
ATCAACG	16040	0.0	30.027891	3
CAACGCA	16180	0.0	29.636356	5
TATCAAC	16840	0.0	29.18536	2
AACGCAG	17015	0.0	28.257118	6
ACGCAGA	19605	0.0	24.458872	7
GTGGTAT	3670	0.0	24.274935	1
CGCAGAG	20175	0.0	23.746716	8
TGGTATC	3600	0.0	23.089256	2
GCAGAGT	23495	0.0	20.336721	9
GAGTACT	12915	0.0	19.224379	12-13
CAGAGTA	22805	0.0	18.260609	10-11
TACATGG	17790	0.0	17.994585	2
GTACATG	18045	0.0	17.976519	1
AGAGTAC	21170	0.0	17.939388	10-11
GTACTTT	13865	0.0	17.83031	14-15
ACATGGG	17985	0.0	17.25328	3
AGTACTT	13810	0.0	16.48156	12-13
>>END_MODULE