FastQCFastQC Report
Fri 27 May 2016
SRR2139454_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139454_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3798924
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51750.052.5919041
GTATCAA97400.037.0965081
CTTATAC33650.035.3694041
TCAACGC118350.030.4192494
ATCAACG119200.030.345473
CAACGCA120700.029.7916535
TATCAAC123700.029.2438622
AACGCAG124000.029.033216
TATACAC47850.028.5260943
TTATACA51350.025.420822
ACGCAGA143750.025.073977
CGCAGAG147950.024.3910058
GTGGTAT25150.022.7287431
TGGTATC24900.021.9290182
ACACATC57600.021.7720416
TACACAT63800.021.6620035
GCAGAGT169200.021.3277159
ACATCTC59450.021.3097768
GAGTACT92600.019.89972312-13
CACATCT64000.019.5948357
Produced by FastQC (version 0.11.5)