##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139454_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3798924
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.481868286914928	18.0	18.0	32.0	18.0	33.0
2	29.6881682813344	32.0	27.0	33.0	25.0	33.0
3	30.98735457724345	33.0	30.0	33.0	28.0	33.0
4	32.12844189565256	33.0	32.0	33.0	31.0	33.0
5	32.53364584287551	33.0	33.0	33.0	32.0	34.0
6	37.024307409150595	38.0	37.0	38.0	35.0	38.0
7	37.01543516006111	38.0	38.0	38.0	35.0	38.0
8	37.33821418907038	38.0	38.0	38.0	37.0	38.0
9	37.45893152903296	38.0	38.0	38.0	37.0	38.0
10-11	37.433163837970966	38.0	38.0	38.0	37.0	38.0
12-13	37.535174433602776	38.0	38.0	38.0	37.5	38.0
14-15	37.543989034789846	38.0	38.0	38.0	37.5	38.0
16-17	37.57282601599822	38.0	38.0	38.0	38.0	38.0
18-19	37.57697929729576	38.0	38.0	38.0	38.0	38.0
20-21	37.58023824570316	38.0	38.0	38.0	38.0	38.0
22-23	37.58736031919894	38.0	38.0	38.0	38.0	38.0
24-25	37.591918738426166	38.0	38.0	38.0	38.0	38.0
26-27	37.56276125633451	38.0	38.0	38.0	38.0	38.0
28-29	37.54317053976388	38.0	38.0	38.0	38.0	38.0
30-31	37.53701790868924	38.0	38.0	38.0	38.0	38.0
32-33	37.44779064920872	38.0	38.0	38.0	37.0	38.0
34-35	37.49587372797719	38.0	38.0	38.0	37.0	38.0
36-37	37.4826909944112	38.0	38.0	38.0	37.5	38.0
38-39	37.4577220088789	38.0	38.0	38.0	37.0	38.0
40-41	37.46171635846805	38.0	38.0	38.0	37.0	38.0
42-43	37.427984732417265	38.0	38.0	38.0	37.0	38.0
44-45	37.39124571425056	38.0	38.0	38.0	37.0	38.0
46-47	37.36015899835871	38.0	38.0	38.0	37.0	38.0
48-49	37.245947154891454	38.0	38.0	38.0	37.0	38.0
50-51	37.23129126917695	38.0	38.0	38.0	37.0	38.0
52-53	37.232537324938434	38.0	38.0	38.0	37.0	38.0
54-55	37.21156655326399	38.0	38.0	38.0	37.0	38.0
56-57	37.15722795893949	38.0	38.0	38.0	36.5	38.0
58-59	37.13745239655266	38.0	38.0	38.0	36.0	38.0
60-61	37.14486365962151	38.0	38.0	38.0	36.0	38.0
62-63	37.1432792278699	38.0	38.0	38.0	36.0	38.0
64-65	37.14613959390398	38.0	38.0	38.0	36.0	38.0
66-67	37.07789507881972	38.0	38.0	38.0	36.0	38.0
68-69	37.09364209940477	38.0	38.0	38.0	36.0	38.0
70-71	37.10681967575276	38.0	38.0	38.0	36.0	38.0
72-73	37.120725807764366	38.0	38.0	38.0	36.0	38.0
74-75	37.10882192404589	38.0	38.0	38.0	36.0	38.0
76-77	37.076180119063096	38.0	38.0	38.0	36.0	38.0
78-79	37.073920865563345	38.0	38.0	38.0	36.0	38.0
80-81	37.07224283446092	38.0	38.0	38.0	36.0	38.0
82-83	37.0167647062124	38.0	38.0	38.0	36.0	38.0
84-85	37.01289185158493	38.0	38.0	38.0	36.0	38.0
86-87	37.004075524224476	38.0	38.0	38.0	36.0	38.0
88-89	36.98288209955863	38.0	38.0	38.0	36.0	38.0
90-91	36.95551643651693	38.0	38.0	38.0	35.5	38.0
92-93	36.94051658230525	38.0	38.0	38.0	35.0	38.0
94-95	36.93223636590734	38.0	38.0	38.0	35.0	38.0
96-97	36.904955968430535	38.0	38.0	38.0	35.0	38.0
98-99	36.92528721358409	38.0	38.0	38.0	35.0	38.0
100	35.416106408764165	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	14.0
21	53.0
22	157.0
23	473.0
24	1177.0
25	2411.0
26	4400.0
27	7837.0
28	12846.0
29	19093.0
30	25686.0
31	32583.0
32	42153.0
33	57108.0
34	88815.0
35	175204.0
36	649517.0
37	2679394.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.705907251632304	23.260612741923765	14.529245649557613	23.504234356886318
2	14.31738385652184	20.487921994398462	41.90787182326957	23.28682232581012
3	18.174488355123714	25.740420182135782	31.010701977717904	25.074389485022603
4	12.972015233787252	18.283598197805485	38.471998913376524	30.27238765503074
5	13.617803356950548	37.26399896391715	35.331951889535034	13.786245789597265
6	29.953323625321275	38.207160764469094	18.370306960602527	13.4692086496071
7	26.676158828131335	32.91008190740325	22.487788647522297	17.92597061694311
8	24.14791662060099	37.51272728804261	20.728185138739285	17.61117095261711
9	25.364471624070394	17.611407861805077	21.30503268820329	35.719087825921235
10-11	24.32782282562115	27.206809085941174	28.380812566926846	20.08455552151083
12-13	25.182999186085326	24.496120480430776	28.751904486638846	21.568975846845053
14-15	22.333889806692632	25.941832476775	26.32998185802085	25.39429585851152
16-17	21.837920421677296	29.37635762126328	26.987957642743048	21.79776431431637
18-19	21.893001807880335	28.138072780608404	28.934074490566275	21.034850920944983
20-21	22.659599671482123	27.196331550351683	28.864981257633833	21.279087520532368
22-23	22.404116538018272	27.30336433846505	28.735002208635212	21.557516914881464
24-25	22.25417988704801	27.451645786098883	28.853791621165385	21.44038270568772
26-27	22.170942254810168	27.582427680281917	28.7965284850221	21.450101579885814
28-29	22.014644735253103	27.402690974382843	28.89280549494298	21.689858795421074
30-31	22.207284670868894	27.42258908426497	28.894103409659415	21.476022835206717
32-33	21.819882369939066	27.44530614217828	29.020213490081847	21.71459799780081
34-35	21.965422063953298	27.395945167759916	28.912820444932784	21.725812323354006
36-37	21.96828867652808	27.478292575134546	29.01276760398875	21.54065114434863
38-39	21.881831144627746	27.464977971301963	29.013968724072637	21.639222159997658
40-41	22.147232137143032	27.331439026239746	28.932573997099116	21.58875483951811
42-43	22.201817558948626	27.44151951045405	28.72302196235103	21.633640968246297
44-45	22.087898052185185	27.62822367898221	28.63560270101132	21.648275567821287
46-47	22.341381684209917	27.462273312299857	28.223179805990977	21.97316519749925
48-49	22.23254023597823	27.620027102927757	28.176742260253278	21.970690400840738
50-51	22.057604218673738	27.710443084860444	28.26805205580395	21.963900640661873
52-53	22.25780269365181	27.697939587016197	28.360622542626395	21.6836351767056
54-55	22.257841986978573	27.735639780482384	28.098107414514338	21.908410818024706
56-57	22.385374388584722	27.948277021372174	27.843497717430647	21.82285087261246
58-59	22.125822275862188	28.118558947302198	28.15718722637701	21.598431550458606
60-61	22.352640448980495	28.394254013368393	27.76712337536124	21.485982162289872
62-63	22.111412822293417	28.682169064671708	27.755662339966985	21.45075577306789
64-65	22.13655881073561	28.5789992053053	27.7251424216254	21.55929956233369
66-67	22.20843587231806	28.732588283079274	27.53153820423376	21.527437640368902
68-69	22.14943631077646	28.268412804169962	27.919793976700895	21.66235690835268
70-71	22.283763160710848	27.968356742449952	27.91086629073468	21.837013806104522
72-73	22.351402006041827	28.000723671840465	27.892620941956853	21.755253380160855
74-75	22.223494011225288	28.098743361573288	27.79877442510632	21.878988202095105
76-77	22.314575387808773	27.733727523716915	27.99341077753203	21.95828631094228
78-79	22.21925059714294	27.74584782530576	27.95333333121327	22.081568246338037
80-81	22.279145796206645	27.804834011372293	27.90409017645076	22.0119300159703
82-83	22.34672048082116	27.70516686981705	27.975990757834516	21.972121891527273
84-85	22.347615923572413	27.725421446026832	27.95770207866009	21.969260551740664
86-87	22.26121587379759	27.74705834849171	27.966225948490646	22.025499829220053
88-89	22.315054592142943	27.714402261590905	27.936248146408253	22.0342949998579
90-91	22.358644201295895	27.748017574980583	27.891771383273344	22.001566840450177
92-93	22.39637916389061	27.717365765518842	27.882075053369466	22.004180017221085
94-95	22.306937496749455	27.77555939179862	27.890773393859273	22.026729717592648
96-97	22.388326371163647	27.863398141389407	27.92068444496083	21.827591042486112
98-99	23.427471785454866	29.766584243039528	28.151832465691285	18.65411150581432
100	22.302208308817885	30.682893760712183	22.130413650200815	24.884484280269113
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	968.0
1	877.5
2	833.0
3	964.0
4	1224.0
5	1597.0
6	1942.5
7	2529.5
8	3328.0
9	3931.5
10	4173.0
11	4296.0
12	4775.0
13	5967.0
14	8559.0
15	11659.5
16	13967.0
17	15290.5
18	15151.5
19	13945.5
20	12624.0
21	11709.0
22	11938.5
23	13778.0
24	17137.5
25	22333.0
26	29207.0
27	36605.5
28	44346.0
29	53061.5
30	61697.0
31	70494.0
32	80331.5
33	90309.0
34	100049.5
35	109369.0
36	119264.0
37	127491.5
38	133635.0
39	139407.5
40	144623.0
41	149356.0
42	151879.0
43	154905.0
44	161821.0
45	170417.0
46	170544.5
47	165249.0
48	161405.0
49	156915.5
50	153353.5
51	148536.5
52	141892.0
53	133668.0
54	124587.5
55	115325.0
56	104849.0
57	93688.0
58	82425.0
59	71324.0
60	59806.5
61	48409.0
62	38431.0
63	29788.0
64	22442.5
65	16429.5
66	12010.0
67	9055.0
68	6880.0
69	5015.0
70	3596.5
71	2574.0
72	1790.0
73	1207.0
74	781.0
75	525.0
76	346.5
77	209.0
78	130.5
79	79.0
80	48.5
81	36.5
82	29.5
83	20.0
84	13.0
85	8.5
86	5.5
87	4.0
88	4.0
89	3.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.008555054010030209
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.237762826889432E-4
26-27	2.764608222761072E-4
28-29	0.002594322043072067
30-31	0.011915810580264386
32-33	0.007197171736006685
34-35	7.016147597505587E-4
36-37	7.972604006605569E-5
38-39	6.948435526335305E-4
40-41	0.0
42-43	0.001420624243940395
44-45	6.815594680401089E-4
46-47	0.0014298930976172893
48-49	0.0026761102391028237
50-51	0.0020406389044732203
52-53	0.0
54-55	0.0014894542384335367
56-57	0.045401863692880344
58-59	0.0633638808574457
60-61	0.04327780511725144
62-63	0.011804696069402813
64-65	0.0
66-67	0.0
68-69	1.509104806574264E-4
70-71	0.0013128289800585858
72-73	0.005546195171071541
74-75	0.008404536703422612
76-77	0.017708980530982923
78-79	0.012877739063301877
80-81	0.005702205757750061
82-83	0.0347580397439199
84-85	0.023070322373346306
86-87	0.0077440723097787776
88-89	0.002189990539575219
90-91	0.003718361183013512
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	26.0
20-21	106.0
22-23	296.0
24-25	345.0
26-27	927.0
28-29	2361.0
30-31	6725.0
32-33	8494.0
34-35	12213.0
36-37	19842.0
38-39	23217.0
40-41	22730.0
42-43	26112.0
44-45	30840.0
46-47	31156.0
48-49	29336.0
50-51	27225.0
52-53	25485.0
54-55	27599.0
56-57	29306.0
58-59	28919.0
60-61	28898.0
62-63	28728.0
64-65	31514.0
66-67	34118.0
68-69	36338.0
70-71	41694.0
72-73	30484.0
74-75	29851.0
76-77	31095.0
78-79	32097.0
80-81	31933.0
82-83	30012.0
84-85	29739.0
86-87	30411.0
88-89	32167.0
90-91	32682.0
92-93	31672.0
94-95	36936.0
96-97	113619.0
98-99	249852.0
100-101	2501824.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.13288351910691
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.80688991296937	83.64952598296247
2	5.396897175091684	9.728758088942719
3	1.0873037144470463	2.9400545713244375
4	0.35369764786946273	1.275191555856015
5	0.1470461309165655	0.6626845894919071
6	0.07537226452252486	0.40761117232680283
7	0.04440233684906808	0.2801477458635241
8	0.02240739190178036	0.16157142754001189
9	0.01589852780809155	0.12896821395467983
>10	0.04883296756858854	0.6638898019457183
>50	0.0010168304523325003	0.0672704176666211
>100	2.3509960346993148E-4	0.03432643212512298
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.6323243107785258E-5	0.0	2.6323243107785258E-5	2.6323243107785258E-5
3	0.0	2.6323243107785258E-5	0.0	2.6323243107785258E-5	2.6323243107785258E-5
4	0.0	2.6323243107785258E-5	0.0	5.2646486215570515E-5	2.6323243107785258E-5
5	0.0	5.2646486215570515E-5	0.0	5.2646486215570515E-5	2.6323243107785258E-5
6	0.0	5.2646486215570515E-5	0.0	5.2646486215570515E-5	2.6323243107785258E-5
7	0.0	7.896972932335577E-5	0.0	5.2646486215570515E-5	2.6323243107785258E-5
8	0.0	7.896972932335577E-5	0.0	5.2646486215570515E-5	2.6323243107785258E-5
9	0.0	1.316162155389263E-4	0.0	5.2646486215570515E-5	2.6323243107785258E-5
10-11	0.0	1.316162155389263E-4	0.0	5.2646486215570515E-5	2.6323243107785258E-5
12-13	0.0	1.316162155389263E-4	0.0	5.2646486215570515E-5	2.6323243107785258E-5
14-15	0.0	1.5793945864671155E-4	0.0	5.2646486215570515E-5	2.6323243107785258E-5
16-17	0.0	1.5793945864671155E-4	0.0	5.2646486215570515E-5	2.6323243107785258E-5
18-19	0.0	1.711010802006042E-4	0.0	5.2646486215570515E-5	6.580810776946314E-5
20-21	0.0	2.1058594486228206E-4	0.0	5.2646486215570515E-5	7.896972932335577E-5
22-23	0.0	2.1058594486228206E-4	0.0	7.896972932335577E-5	9.21313508772484E-5
24-25	0.0	2.3690918797006732E-4	0.0	1.0529297243114103E-4	1.0529297243114103E-4
26-27	0.0	2.3690918797006732E-4	0.0	1.316162155389263E-4	1.0529297243114103E-4
28-29	0.0	2.3690918797006732E-4	0.0	3.9484864661677884E-4	1.0529297243114103E-4
30-31	0.0	2.895556741856378E-4	0.0	0.0011450610751886586	1.0529297243114103E-4
32-33	0.0	2.895556741856378E-4	0.0	0.0027112940401018814	1.0529297243114103E-4
34-35	0.0	3.6852540350899355E-4	0.0	0.004843476731832487	1.0529297243114103E-4
36-37	0.0	4.738183759401346E-4	0.0	0.008186528606521215	1.0529297243114103E-4
38-39	0.0	5.001416190479199E-4	0.0	0.013135298310784842	1.316162155389263E-4
40-41	0.0	5.527881052634904E-4	0.0	0.01871582584963532	1.316162155389263E-4
42-43	0.0	5.922729699251683E-4	0.0	0.02387518149876123	1.316162155389263E-4
44-45	0.0	6.975659423563093E-4	0.0	0.02954784038848895	1.316162155389263E-4
46-47	0.0	7.370508070179872E-4	0.0	0.03544424684463285	1.447778370928189E-4
48-49	0.0	7.370508070179872E-4	0.0	0.04109058249125279	1.842627017544968E-4
50-51	0.0	7.896972932335577E-4	0.0	0.046671110030103255	1.842627017544968E-4
52-53	0.0	7.896972932335577E-4	0.0	0.05189627378699863	2.237475664161747E-4
54-55	0.0	7.896972932335577E-4	0.0	0.057713710513819176	2.632324310778526E-4
56-57	0.0	8.16020536341343E-4	0.0	0.06375489480705589	2.763940526317452E-4
58-59	0.0	8.291821578952357E-4	0.0	0.06995401855893932	2.895556741856378E-4
60-61	0.0	8.423437794491282E-4	0.0	0.07592939474440658	2.895556741856378E-4
62-63	0.0	8.555054010030208E-4	0.0	0.08161521525568818	3.158789172934231E-4
64-65	0.0	9.739599949880546E-4	0.0	0.08682721739102967	3.290405388473157E-4
66-67	0.0	9.739599949880546E-4	0.0	0.09207870439103283	3.5536378195510096E-4
68-69	0.0	9.739599949880546E-4	0.0	0.09739599949880545	3.685254035089936E-4
70-71	0.0	9.871216165419471E-4	0.0	0.10173933461159002	3.9484864661677884E-4
72-73	0.0	0.0010002832380958397	0.0	0.1054903967544494	5.001416190479199E-4
74-75	0.0	0.0010134448596497325	0.0	0.1085570545765064	5.264648621557052E-4
76-77	0.0	0.0010397681027575177	0.0	0.1115710659123478	5.264648621557052E-4
78-79	0.0	0.0010529297243114103	0.0	0.1135453091454317	5.264648621557052E-4
80-81	0.0	0.0010529297243114103	0.0	0.11422971346623412	5.264648621557052E-4
82-83	0.0	0.0010529297243114103	0.0	0.11476933994994372	5.527881052634904E-4
84-85	0.0	0.0010529297243114103	0.0	0.11488779454392875	5.527881052634904E-4
86-87	0.0	0.0010529297243114103	0.0	0.11508521886723715	5.527881052634904E-4
88	0.0	0.001131899453634766	0.0	0.11508521886723715	5.527881052634904E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5175	0.0	52.591904	1
GTATCAA	9740	0.0	37.096508	1
CTTATAC	3365	0.0	35.369404	1
TCAACGC	11835	0.0	30.419249	4
ATCAACG	11920	0.0	30.34547	3
CAACGCA	12070	0.0	29.791653	5
TATCAAC	12370	0.0	29.243862	2
AACGCAG	12400	0.0	29.03321	6
TATACAC	4785	0.0	28.526094	3
TTATACA	5135	0.0	25.42082	2
ACGCAGA	14375	0.0	25.07397	7
CGCAGAG	14795	0.0	24.391005	8
GTGGTAT	2515	0.0	22.728743	1
TGGTATC	2490	0.0	21.929018	2
ACACATC	5760	0.0	21.772041	6
TACACAT	6380	0.0	21.662003	5
GCAGAGT	16920	0.0	21.327715	9
ACATCTC	5945	0.0	21.309776	8
GAGTACT	9260	0.0	19.899723	12-13
CACATCT	6400	0.0	19.594835	7
>>END_MODULE