FastQCFastQC Report
Fri 27 May 2016
SRR2139453_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139453_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5446553
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73000.048.477171
GTATCAA133000.037.283131
TCAACGC159300.031.366394
ATCAACG161100.031.042553
CAACGCA165100.030.1865465
TATCAAC166800.030.0367552
AACGCAG170350.029.2310526
GTGGTAT37050.026.2811181
TGGTATC35100.026.0302732
ACGCAGA198300.025.110997
CGCAGAG202800.024.5537958
GCAGAGT233850.021.2569249
GAGTACT123900.019.8352512-13
CAGAGTA229400.018.63108810-11
GTACTTT135850.018.05912414-15
AGAGTAC214450.017.89993110-11
GTACATG179250.016.918681
TACATGG179000.016.9183442
AGTACTT136850.016.54792212-13
ACATGGG180100.016.4796033
Produced by FastQC (version 0.11.5)