##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139453_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5446553
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.56652455231777	18.0	18.0	32.0	18.0	33.0
2	29.820316813221133	32.0	27.0	33.0	25.0	33.0
3	31.07367246770572	33.0	31.0	33.0	28.0	33.0
4	32.15393662744125	33.0	32.0	33.0	31.0	33.0
5	32.55474701155024	33.0	33.0	33.0	32.0	34.0
6	37.03912547991363	38.0	37.0	38.0	36.0	38.0
7	37.02899081308857	38.0	38.0	38.0	35.0	38.0
8	37.35004304557396	38.0	38.0	38.0	37.0	38.0
9	37.46550065702105	38.0	38.0	38.0	37.0	38.0
10-11	37.43872124259141	38.0	38.0	38.0	37.0	38.0
12-13	37.53535961184991	38.0	38.0	38.0	37.5	38.0
14-15	37.5432376220336	38.0	38.0	38.0	37.5	38.0
16-17	37.57448986542498	38.0	38.0	38.0	38.0	38.0
18-19	37.574368504263155	38.0	38.0	38.0	38.0	38.0
20-21	37.5780777607272	38.0	38.0	38.0	38.0	38.0
22-23	37.584963516893716	38.0	38.0	38.0	38.0	38.0
24-25	37.59162458973496	38.0	38.0	38.0	38.0	38.0
26-27	37.56349169474794	38.0	38.0	38.0	38.0	38.0
28-29	37.54120663057035	38.0	38.0	38.0	38.0	38.0
30-31	37.53690045409529	38.0	38.0	38.0	38.0	38.0
32-33	37.44646019618058	38.0	38.0	38.0	37.0	38.0
34-35	37.49778710485933	38.0	38.0	38.0	37.5	38.0
36-37	37.48797284234011	38.0	38.0	38.0	37.5	38.0
38-39	37.4774427558642	38.0	38.0	38.0	38.0	38.0
40-41	37.46371837546039	38.0	38.0	38.0	38.0	38.0
42-43	37.4286435152555	38.0	38.0	38.0	37.0	38.0
44-45	37.39219743547136	38.0	38.0	38.0	37.0	38.0
46-47	37.363023961218765	38.0	38.0	38.0	37.0	38.0
48-49	37.24747999790926	38.0	38.0	38.0	37.0	38.0
50-51	37.23510120635311	38.0	38.0	38.0	37.0	38.0
52-53	37.240987657805704	38.0	38.0	38.0	37.0	38.0
54-55	37.22411600215358	38.0	38.0	38.0	37.0	38.0
56-57	37.168933198152075	38.0	38.0	38.0	36.5	38.0
58-59	37.15145613542353	38.0	38.0	38.0	36.0	38.0
60-61	37.16383032347548	38.0	38.0	38.0	36.0	38.0
62-63	37.166702059484024	38.0	38.0	38.0	36.0	38.0
64-65	37.1702149054649	38.0	38.0	38.0	36.0	38.0
66-67	37.10161689792918	38.0	38.0	38.0	36.0	38.0
68-69	37.12012339372323	38.0	38.0	38.0	36.0	38.0
70-71	37.13285915907577	38.0	38.0	38.0	36.0	38.0
72-73	37.14529545301963	38.0	38.0	38.0	36.0	38.0
74-75	37.12876414742471	38.0	38.0	38.0	36.0	38.0
76-77	37.09615635272705	38.0	38.0	38.0	36.0	38.0
78-79	37.095010883350554	38.0	38.0	38.0	36.0	38.0
80-81	37.09200988670078	38.0	38.0	38.0	36.0	38.0
82-83	37.03323059983495	38.0	38.0	38.0	36.0	38.0
84-85	37.028911397475795	38.0	38.0	38.0	36.0	38.0
86-87	37.01932174336408	38.0	38.0	38.0	36.0	38.0
88-89	36.99661435889293	38.0	38.0	38.0	36.0	38.0
90-91	36.96675602607755	38.0	38.0	38.0	35.5	38.0
92-93	36.950256383380434	38.0	38.0	38.0	35.0	38.0
94-95	36.942543113741934	38.0	38.0	38.0	35.0	38.0
96-97	36.91675133570868	38.0	38.0	38.0	35.0	38.0
98-99	36.93772797661196	38.0	38.0	38.0	35.0	38.0
100	35.52620677931675	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	18.0
21	52.0
22	223.0
23	712.0
24	1696.0
25	3475.0
26	6418.0
27	11009.0
28	18156.0
29	27238.0
30	36408.0
31	47161.0
32	59863.0
33	80754.0
34	124628.0
35	243567.0
36	894837.0
37	3890333.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.30645676265337	22.872080745381528	14.549863005096986	23.271599486868116
2	14.571979534517506	20.557530892125815	41.432741687183075	23.437747886173607
3	18.436004156026137	25.49950436505758	30.601450569892975	25.46304090902331
4	13.170568614681615	17.968942925920302	38.45999478936494	30.40049367003314
5	13.896807760798435	37.162531972056456	35.01350303577327	13.927157231371842
6	30.47217203247632	37.874431773637376	18.277486696631794	13.3759094972545
7	27.16204175374774	32.83537312498382	22.42879120059972	17.573793920668724
8	24.505370644515896	37.297075783527674	20.48148618034195	17.716067391614477
9	25.603055730844808	17.776601090634756	21.314765503980222	35.30557767454021
10-11	24.572220264817034	27.184936968390833	28.116113071882342	20.12672969490979
12-13	25.332526829354272	24.580041725472974	28.58606167974497	21.501369765427786
14-15	22.461031775510126	26.011993273543837	26.33446328347489	25.192511667471152
16-17	21.959090455926898	29.21704792003309	27.017867998346844	21.80599362569317
18-19	21.939353201924224	28.226448911816338	28.8983968392486	20.935801047010834
20-21	22.784241068461387	27.272547840938177	28.872542773450625	21.07066831714981
22-23	22.572711940294536	27.123396505332042	28.71496328174052	21.588928272632906
24-25	22.247553267957386	27.291672347559942	28.79705627357456	21.663718110908114
26-27	22.164881289669623	27.44106125596476	28.76591410595205	21.628143348413566
28-29	22.1199755918575	27.481674373590632	28.790282230400116	21.608067804151755
30-31	22.382784010098238	27.365155499061935	28.68697200439444	21.565088486445394
32-33	22.14905276795452	27.568799179694736	28.59958817079425	21.6825598815565
34-35	22.318760602700163	27.48303914259956	28.656800932368125	21.54139932233215
36-37	22.30018517591712	27.38554404462017	28.634823452650387	21.679447326812323
38-39	22.13838094339097	27.604139245499308	28.70021872947926	21.55726108163046
40-41	22.37106307978606	27.46815541899284	28.529800827813794	21.6309806734073
42-43	22.258110828064652	27.575768380584986	28.384573169270777	21.781547622079586
44-45	22.309288185000735	27.65132357503267	28.280832307573174	21.75855593239342
46-47	22.403231006317057	27.67540285783678	28.01457037838625	21.906795757459918
48-49	22.348099313766788	27.81410626873261	27.93677107100504	21.901023346495563
50-51	22.348740237765043	27.916657824282982	27.86081938816416	21.873782549787812
52-53	22.518483542149557	27.857099367526516	27.7263083732022	21.89810871712173
54-55	22.484910330967462	27.79126784029223	27.772110744602735	21.95171108413757
56-57	22.463782112231396	27.946380302009043	27.729306944296482	21.860530641463072
58-59	22.463264983475074	27.936841418458226	27.73401524257884	21.865878355487855
60-61	22.51039576599974	27.969661349380186	27.765453834887627	21.754489049732445
62-63	22.477979736006525	28.050369012997876	27.742724841099943	21.728926409895653
64-65	22.462512418844298	28.009488382123028	27.707287519029393	21.820711680003285
66-67	22.596702825040772	28.26431108224386	27.3975101511024	21.74147594161297
68-69	22.46837273813882	27.90834740834292	27.744961035179973	21.87831881833829
70-71	22.54530394862119	27.658368209544953	27.752893588344605	22.043434253489245
72-73	22.574784443712954	27.90292886068391	27.65633731656345	21.865949379039687
74-75	22.368339925911638	28.0274882046123	27.638570941907858	21.965600927568207
76-77	22.48239660242147	27.63551116120646	27.796317097231604	22.085775139140466
78-79	22.421069542416475	27.676692860051833	27.70737091272831	22.194866684803376
80-81	22.463112606859827	27.712498060502615	27.679635503515577	22.14475382912198
82-83	22.499547496192008	27.616633204590197	27.800920601593372	22.082898697624426
84-85	22.50792784004732	27.59618987068721	27.77981922804853	22.11606306121694
86-87	22.412293678229247	27.6480021490953	27.775389137811647	22.164315034863808
88-89	22.475271434554646	27.627842536340204	27.739640030170087	22.157245998935064
90-91	22.516356899786103	27.719461197551254	27.73863038904893	22.025551513613713
92-93	22.58671043706732	27.637707219203644	27.64916993012776	22.12641241360128
94-95	22.398940220089948	27.718718522266727	27.69710676774221	22.185234489901116
96-97	22.550920492520092	27.809818090772893	27.751953485547293	21.887307931159725
98-99	23.60891628049016	29.705963639358846	27.956235027053296	18.728885053097706
100	30.00314137002318	40.8080346271434	29.188824002833417	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1425.0
1	1290.5
2	1162.0
3	1261.5
4	1562.5
5	2055.5
6	2545.5
7	3349.0
8	4403.0
9	5185.5
10	5560.0
11	5896.0
12	6682.0
13	8370.0
14	11969.0
15	16110.0
16	19091.5
17	20812.0
18	20802.5
19	19441.0
20	17846.0
21	16605.0
22	16851.5
23	19560.0
24	24472.5
25	31756.5
26	41664.5
27	52875.5
28	63710.0
29	75784.5
30	87833.5
31	99594.0
32	113516.5
33	128008.5
34	141316.5
35	153920.0
36	167974.5
37	179798.0
38	189028.0
39	197200.0
40	205944.0
41	213644.5
42	217735.0
43	221796.0
44	225639.5
45	228446.0
46	229587.5
47	229644.5
48	228834.0
49	227164.0
50	223288.0
51	216264.0
52	206679.5
53	195096.0
54	182122.5
55	168867.5
56	153618.0
57	137638.0
58	122226.0
59	104838.5
60	87826.5
61	71113.0
62	55628.5
63	42547.5
64	31623.5
65	23626.0
66	17443.0
67	13075.0
68	9984.5
69	7376.5
70	5355.5
71	3827.5
72	2634.5
73	1700.5
74	1078.5
75	713.0
76	473.5
77	303.5
78	212.0
79	141.0
80	97.5
81	62.0
82	34.5
83	20.5
84	14.0
85	10.5
86	7.0
87	3.0
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00927191932218414
3	7.344094512621102E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.8362449598518813E-4
26-27	3.397470849240996E-4
28-29	0.002663771178014225
30-31	0.012641424209513356
32-33	0.007903224008778657
34-35	5.909912798313237E-4
36-37	5.560835259699348E-5
38-39	7.268255178002831E-4
40-41	1.875002578128545E-5
42-43	0.001650710272332294
44-45	7.126032918471532E-4
46-47	0.001599956772425406
48-49	0.0032551140062629554
50-51	0.0020442524188373384
52-53	0.0
54-55	0.0017774308934868613
56-57	0.04943756805377788
58-59	0.06881273456891981
60-61	0.047506125590300013
62-63	0.013899434331745463
64-65	0.0
66-67	0.0
68-69	2.2973871606968017E-4
70-71	0.0018651817150651465
72-73	0.006113114736146804
74-75	0.009628179644677284
76-77	0.020065609675972314
78-79	0.014217566994278577
80-81	0.006197185397181692
82-83	0.03900314073820709
84-85	0.026768366982399292
86-87	0.008416883951588626
88-89	0.0027493248116483393
90-91	0.004155467257985413
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	40.0
20-21	160.0
22-23	369.0
24-25	538.0
26-27	1364.0
28-29	3279.0
30-31	9865.0
32-33	12409.0
34-35	17368.0
36-37	27279.0
38-39	32658.0
40-41	32020.0
42-43	36332.0
44-45	42875.0
46-47	43039.0
48-49	40969.0
50-51	37592.0
52-53	35529.0
54-55	38509.0
56-57	39615.0
58-59	40182.0
60-61	38532.0
62-63	37327.0
64-65	38895.0
66-67	41154.0
68-69	42876.0
70-71	42853.0
72-73	39523.0
74-75	40479.0
76-77	42854.0
78-79	44617.0
80-81	44310.0
82-83	41612.0
84-85	41290.0
86-87	42469.0
88-89	44805.0
90-91	45131.0
92-93	44741.0
94-95	51774.0
96-97	165548.0
98-99	1276563.0
100-101	2747209.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.73447725970833
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.89644226826577	81.54382766701528
2	6.024018448985899	10.690762561472052
3	1.2191347211975967	3.24537846583887
4	0.4074237823705259	1.4461014540728714
5	0.19126962669177108	0.8486105170076931
6	0.08500871162272974	0.452592215301853
7	0.056520110414997826	0.351069771663508
8	0.031861627658462226	0.2261779899933688
9	0.021236796106752272	0.16959924010833044
>10	0.06541090287194885	0.8881997031580522
>50	0.001344410555354236	0.08095001848311742
>100	2.994405786049799E-4	0.04281315479138154
>500	2.9152679621256948E-5	0.01391724109358649
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.672047256310551E-5
2	0.0	5.5080708844658265E-5	0.0	0.0	3.672047256310551E-5
3	0.0	5.5080708844658265E-5	0.0	1.8360236281552755E-5	3.672047256310551E-5
4	0.0	1.2852165397086928E-4	0.0	1.8360236281552755E-5	3.672047256310551E-5
5	0.0	1.2852165397086928E-4	0.0	1.8360236281552755E-5	3.672047256310551E-5
6	1.8360236281552755E-5	1.2852165397086928E-4	0.0	3.672047256310551E-5	5.5080708844658265E-5
7	1.8360236281552755E-5	1.2852165397086928E-4	0.0	3.672047256310551E-5	5.5080708844658265E-5
8	1.8360236281552755E-5	1.2852165397086928E-4	0.0	3.672047256310551E-5	5.5080708844658265E-5
9	1.8360236281552755E-5	2.3868307166018582E-4	0.0	9.180118140776377E-5	5.5080708844658265E-5
10-11	1.8360236281552755E-5	2.3868307166018582E-4	0.0	9.180118140776377E-5	5.5080708844658265E-5
12-13	1.8360236281552755E-5	2.3868307166018582E-4	0.0	9.180118140776377E-5	5.5080708844658265E-5
14-15	1.8360236281552755E-5	3.304842530679496E-4	0.0	1.0098129954854015E-4	9.180118140776378E-5
16-17	3.672047256310551E-5	3.304842530679496E-4	0.0	1.1016141768931653E-4	1.1016141768931653E-4
18-19	3.672047256310551E-5	3.4884448934950234E-4	0.0	1.1016141768931653E-4	1.1016141768931653E-4
20-21	3.672047256310551E-5	3.8556496191260786E-4	0.0	1.1016141768931653E-4	1.1934153583009291E-4
22-23	3.672047256310551E-5	4.3146555261648975E-4	0.0	1.1934153583009291E-4	1.560620083931984E-4
24-25	3.672047256310551E-5	4.6818602517959527E-4	0.0	1.4688189025242204E-4	1.8360236281552753E-4
26-27	3.672047256310551E-5	4.957263796019244E-4	0.0	1.7442224467475117E-4	1.8360236281552753E-4
28-29	3.672047256310551E-5	4.957263796019244E-4	0.0	4.039251981941606E-4	1.8360236281552753E-4
30-31	3.672047256310551E-5	5.875275610096882E-4	0.0	0.0010740738224708363	1.8360236281552753E-4
32-33	3.672047256310551E-5	5.875275610096882E-4	0.0	0.0025153523705727272	2.019625990970803E-4
34-35	3.672047256310551E-5	7.252293331213338E-4	0.0	0.0046635000155144	2.2032283537863306E-4
36-37	3.672047256310551E-5	8.445708689514267E-4	0.0	0.007940802191771566	2.3868307166018582E-4
38-39	3.672047256310551E-5	8.904714596553086E-4	0.0	0.012411519726329662	2.3868307166018582E-4
40-41	3.672047256310551E-5	9.914527592038487E-4	0.0	0.017524845530742107	2.3868307166018582E-4
42-43	3.672047256310551E-5	0.0010373533499077306	0.0	0.022546370153746782	2.3868307166018582E-4
44-45	3.672047256310551E-5	0.0012393159490048109	0.0	0.027714776667003883	2.66223426082515E-4
46-47	3.672047256310551E-5	0.0013770177211164566	0.0	0.03317694696076583	3.121240167863968E-4
48-49	3.672047256310551E-5	0.0013770177211164566	0.0	0.039235824933678234	3.121240167863968E-4
50-51	3.672047256310551E-5	0.0015514399657912078	0.0	0.0446520946367363	3.121240167863968E-4
52-53	3.672047256310551E-5	0.001578980320213537	0.0	0.05027032693889144	3.121240167863968E-4
54-55	3.672047256310551E-5	0.001578980320213537	0.0	0.05527349132561457	3.121240167863968E-4
56-57	3.672047256310551E-5	0.0015973405564950896	0.0	0.06110286634500756	3.121240167863968E-4
58-59	3.672047256310551E-5	0.0016157007927766424	0.0	0.06717092443606076	3.121240167863968E-4
60-61	4.590059070388189E-5	0.0016707815016213006	0.0	0.07330324335409938	3.121240167863968E-4
62-63	7.344094512621102E-5	0.0017442224467475117	0.0	0.0789673762469584	3.121240167863968E-4
64-65	7.344094512621102E-5	0.0019829055184076975	0.0	0.08487019221147761	3.121240167863968E-4
66-67	7.344094512621102E-5	0.0019829055184076975	0.0	0.09082808888484148	3.121240167863968E-4
68-69	7.344094512621102E-5	0.0020104458728300266	0.0	0.09620763811533643	3.121240167863968E-4
70-71	7.344094512621102E-5	0.002019625990970803	0.0	0.10094457907597704	3.121240167863968E-4
72-73	9.180118140776377E-5	0.002019625990970803	0.0	0.1045431853871614	3.4884448934950234E-4
74-75	9.180118140776377E-5	0.002074706699815461	0.0	0.10749000331035061	3.4884448934950234E-4
76-77	1.0098129954854015E-4	0.002175687999364001	0.0	0.11051026217866602	3.4884448934950234E-4
78-79	1.1016141768931653E-4	0.00223076870820866	0.0	0.11237382616124364	3.4884448934950234E-4
80-81	1.1016141768931653E-4	0.002239948826349436	0.0	0.1131633163213504	3.947450800533842E-4
82-83	1.1016141768931653E-4	0.002239948826349436	0.0	0.11356724151954456	4.406456707572661E-4
84-85	1.1016141768931653E-4	0.002239948826349436	0.0	0.11377838423678242	4.406456707572661E-4
86-87	1.1016141768931653E-4	0.002239948826349436	0.0	0.11384264506376784	4.406456707572661E-4
88	1.1016141768931653E-4	0.0022766692989125417	0.0	0.11385182518190863	4.406456707572661E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7300	0.0	48.47717	1
GTATCAA	13300	0.0	37.28313	1
TCAACGC	15930	0.0	31.36639	4
ATCAACG	16110	0.0	31.04255	3
CAACGCA	16510	0.0	30.186546	5
TATCAAC	16680	0.0	30.036755	2
AACGCAG	17035	0.0	29.231052	6
GTGGTAT	3705	0.0	26.281118	1
TGGTATC	3510	0.0	26.030273	2
ACGCAGA	19830	0.0	25.11099	7
CGCAGAG	20280	0.0	24.553795	8
GCAGAGT	23385	0.0	21.256924	9
GAGTACT	12390	0.0	19.83525	12-13
CAGAGTA	22940	0.0	18.631088	10-11
GTACTTT	13585	0.0	18.059124	14-15
AGAGTAC	21445	0.0	17.899931	10-11
GTACATG	17925	0.0	16.91868	1
TACATGG	17900	0.0	16.918344	2
AGTACTT	13685	0.0	16.547922	12-13
ACATGGG	18010	0.0	16.479603	3
>>END_MODULE