FastQCFastQC Report
Fri 27 May 2016
SRR2139452_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139452_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3944402
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55300.049.1350361
GTATCAA101200.037.2183531
TCAACGC121850.031.4336434
ATCAACG122550.031.4281183
CAACGCA124750.030.702925
TATCAAC127150.030.595352
AACGCAG128650.029.8716326
ACGCAGA147900.025.9836737
CGCAGAG151050.025.413578
GCAGAGT174950.021.8930519
TGGTATC27050.021.130732
GAGTACT101700.020.40355312-13
GTGGTAT30400.019.9246961
CAGAGTA173250.019.21514110-11
GTACTTT110750.018.58233314-15
AGAGTAC161450.017.91164810-11
GTACATG125450.017.3410931
TACATGG128450.016.9028782
ACATGGG129300.016.3286613
AGTACTT110650.016.30545212-13
Produced by FastQC (version 0.11.5)