##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139452_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3944402
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.45244881226609	18.0	18.0	32.0	18.0	33.0
2	29.676753282246587	32.0	27.0	33.0	25.0	33.0
3	31.014428803149375	33.0	30.0	33.0	28.0	33.0
4	32.14808100188571	33.0	32.0	33.0	31.0	33.0
5	32.560688793890684	33.0	33.0	33.0	32.0	34.0
6	37.03592331613258	38.0	37.0	38.0	35.0	38.0
7	37.04087058063554	38.0	38.0	38.0	35.0	38.0
8	37.34625426110219	38.0	38.0	38.0	37.0	38.0
9	37.46495058059498	38.0	38.0	38.0	37.0	38.0
10-11	37.443425517987265	38.0	38.0	38.0	37.0	38.0
12-13	37.54059931011089	38.0	38.0	38.0	37.5	38.0
14-15	37.553254587133864	38.0	38.0	38.0	37.5	38.0
16-17	37.58319372619728	38.0	38.0	38.0	38.0	38.0
18-19	37.584605727306695	38.0	38.0	38.0	38.0	38.0
20-21	37.587437137643036	38.0	38.0	38.0	38.0	38.0
22-23	37.59559344932526	38.0	38.0	38.0	38.0	38.0
24-25	37.60172946047837	38.0	38.0	38.0	38.0	38.0
26-27	37.57530086452985	38.0	38.0	38.0	38.0	38.0
28-29	37.55416016283179	38.0	38.0	38.0	38.0	38.0
30-31	37.547913224129886	38.0	38.0	38.0	38.0	38.0
32-33	37.46188712351207	38.0	38.0	38.0	38.0	38.0
34-35	37.506289963420876	38.0	38.0	38.0	38.0	38.0
36-37	37.49323221288503	38.0	38.0	38.0	38.0	38.0
38-39	37.48131456005291	38.0	38.0	38.0	38.0	38.0
40-41	37.46539908490761	38.0	38.0	38.0	38.0	38.0
42-43	37.42775657222018	38.0	38.0	38.0	37.0	38.0
44-45	37.38817913206696	38.0	38.0	38.0	37.0	38.0
46-47	37.35533741317305	38.0	38.0	38.0	37.0	38.0
48-49	37.24365659544218	38.0	38.0	38.0	37.0	38.0
50-51	37.22786321119197	38.0	38.0	38.0	37.0	38.0
52-53	37.231078009335626	38.0	38.0	38.0	37.0	38.0
54-55	37.21346293532044	38.0	38.0	38.0	37.0	38.0
56-57	37.16221089652038	38.0	38.0	38.0	36.5	38.0
58-59	37.14414990898044	38.0	38.0	38.0	36.0	38.0
60-61	37.15665322639387	38.0	38.0	38.0	36.0	38.0
62-63	37.1575427584245	38.0	38.0	38.0	36.0	38.0
64-65	37.16085747384055	38.0	38.0	38.0	36.0	38.0
66-67	37.098700722388706	38.0	38.0	38.0	36.0	38.0
68-69	37.1144324722196	38.0	38.0	38.0	36.0	38.0
70-71	37.12727000431908	38.0	38.0	38.0	36.0	38.0
72-73	37.14217390267679	38.0	38.0	38.0	36.0	38.0
74-75	37.12745677608825	38.0	38.0	38.0	36.0	38.0
76-77	37.095984559707084	38.0	38.0	38.0	36.0	38.0
78-79	37.09317843527346	38.0	38.0	38.0	36.0	38.0
80-81	37.09132547033212	38.0	38.0	38.0	36.0	38.0
82-83	37.03230713429767	38.0	38.0	38.0	36.0	38.0
84-85	37.02790402736576	38.0	38.0	38.0	36.0	38.0
86-87	37.01994034162323	38.0	38.0	38.0	36.0	38.0
88-89	36.99687050121867	38.0	38.0	38.0	36.0	38.0
90-91	36.9709325232201	38.0	38.0	38.0	35.5	38.0
92-93	36.95578983258521	38.0	38.0	38.0	35.0	38.0
94-95	36.94788652306461	38.0	38.0	38.0	35.0	38.0
96-97	36.92202646254581	38.0	38.0	38.0	35.0	38.0
98-99	36.94053863276263	38.0	38.0	38.0	35.0	38.0
100	35.530327941812914	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	11.0
21	38.0
22	167.0
23	429.0
24	1105.0
25	2368.0
26	4528.0
27	7921.0
28	13445.0
29	20190.0
30	26449.0
31	33255.0
32	42503.0
33	57552.0
34	89400.0
35	176167.0
36	663932.0
37	2804939.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.16829471235437	23.035304210879115	14.519767508484177	23.27663356828234
2	14.445851604225535	20.419688573989347	41.55718076171714	23.577279060067973
3	18.28389615657641	25.589924956900923	30.71186999290133	25.414308893621335
4	13.040785396620326	17.871504983518413	38.810547200817766	30.277162419043496
5	13.77927503332571	36.91079154710904	35.27404154039066	14.035891879174587
6	30.135214412729738	37.81926892847129	18.52610357666384	13.519413082135138
7	26.873072267988913	32.61962142803903	22.88012225934375	17.62718404462831
8	24.353957836954756	37.00137562043626	20.79047723837479	17.854189304234204
9	25.419493246378032	17.501689736492377	21.618181919591358	35.46063509753824
10-11	24.498593196129605	26.959574607253522	28.363095343730176	20.1787368528867
12-13	25.323534467328635	24.308792562218557	28.875188178081242	21.49248479237157
14-15	22.434947046472445	25.692525761826506	26.65979532512153	25.212731866579524
16-17	21.81472628804062	28.874072673120033	27.337109655658832	21.974091383180514
18-19	21.798754285186956	27.856871586618198	29.315064742386802	21.02930938580804
20-21	22.653733251545592	26.91753395008793	29.282634569162298	21.14609822920418
22-23	22.421138500992917	26.79424290615724	29.154494187951734	21.63012440489811
24-25	22.11502292747807	26.949716755093405	29.228480759339515	21.70677955808901
26-27	22.042090803124395	27.089378576189745	29.167266304039796	21.701264316646064
28-29	21.984817062729505	27.141485441757517	29.207569905566444	21.666127589946534
30-31	22.274765137654285	26.968977474291794	29.12163577703599	21.63462161101793
32-33	22.01877414668463	27.165979078491908	29.052972912460675	21.762273862362786
34-35	22.20263853144509	27.163845862793355	29.06487090130929	21.568644704452264
36-37	22.188774823845435	27.029852677706867	29.03144011359088	21.74993238485682
38-39	22.010562625084226	27.207256243300844	29.094761608421265	21.68741952319367
40-41	22.301945371826932	27.06956593057828	28.927712624542316	21.700776073052474
42-43	22.17574765968779	27.16317983556064	28.786655284289473	21.874417220462092
44-45	22.217443144193545	27.28878961022304	28.630208039705856	21.863559205877557
46-47	22.29166056282441	27.320877473779554	28.361025222777535	22.0264367406185
48-49	22.313461990367372	27.381992571501407	28.273808289847064	22.030737148284157
50-51	22.278426148028004	27.512983700232184	28.171045677568372	22.037544474171437
52-53	22.400992251567516	27.44853401570558	28.070647920272023	22.079825812454885
54-55	22.38861387155256	27.426259407961407	28.077283355051474	22.107843365434558
56-57	22.34699927947026	27.522744740533344	28.115255484269724	22.01500049572667
58-59	22.352322110529176	27.486368853727395	28.144432836093223	22.01687619965021
60-61	22.43171809631767	27.45549509707754	28.195255216398586	21.917531590206206
62-63	22.402541426422715	27.515501732983765	28.155727622456116	21.9262292181374
64-65	22.33515526521474	27.424677094789978	28.17369987742952	22.066467762565757
66-67	22.419575392217112	27.529889086731508	28.029903554242875	22.02063196680851
68-69	22.3224270828021	27.25029774501287	28.37136202233847	22.05591314984656
70-71	22.46337993832874	27.152340806033	28.201899305868274	22.182379949769988
72-73	22.465453601062404	27.41691533124669	28.12108781752578	21.996543250165132
74-75	22.305894832776794	27.531222219732115	28.037306798741994	22.125576148749094
76-77	22.432074896002494	27.298544374255584	28.074281843689043	22.19509888605288
78-79	22.32731621526985	27.400608956575102	27.976149226193204	22.295925601961844
80-81	22.36759566683022	27.473011544379876	27.99717141075427	22.16222137803563
82-83	22.485608740893284	27.359817468447435	28.00873977391572	22.145834016743564
84-85	22.500749919828035	27.374577380345993	27.916780011430486	22.20789268839548
86-87	22.351798447251184	27.40473767629043	27.994201558137117	22.24926231832127
88-89	22.42779883813431	27.424570544029592	27.93333304516426	22.21429757267184
90-91	22.478118333669396	27.47244300150792	27.874571113514087	22.174867551308594
92-93	22.550432017349404	27.44137164485939	27.865601655365758	22.142594682425447
94-95	22.35044889813506	27.5336872111674	27.875307029384828	22.24055686131271
96-97	22.52794631350614	27.621660766282623	27.866771736628365	21.983621183582873
98-99	23.61159736246377	29.521298172233344	28.08762651685467	18.77947794844822
100	30.04146720019153	40.596419681215146	29.36211311859333	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	928.0
1	909.5
2	965.5
3	1146.5
4	1487.0
5	2017.5
6	2479.5
7	3095.5
8	3933.0
9	4444.5
10	4544.0
11	4733.5
12	5399.5
13	6834.0
14	9741.0
15	13232.0
16	15856.0
17	17282.0
18	17090.5
19	15674.0
20	14185.0
21	13124.5
22	13143.5
23	14819.0
24	18063.5
25	23154.5
26	30219.5
27	38346.0
28	46466.0
29	55397.0
30	64370.0
31	73008.0
32	82183.0
33	92261.5
34	102484.0
35	111616.5
36	121298.5
37	129938.5
38	136962.5
39	142633.5
40	147962.0
41	152815.0
42	155376.5
43	158460.5
44	162359.5
45	164747.5
46	165067.5
47	165808.0
48	166921.0
49	165379.5
50	161736.0
51	157575.0
52	151180.5
53	142717.5
54	133663.5
55	123567.5
56	112481.5
57	101082.0
58	89428.5
59	76729.0
60	64077.0
61	51887.0
62	40900.0
63	31465.0
64	23270.0
65	17194.0
66	12933.0
67	9911.0
68	7516.5
69	5575.0
70	4034.5
71	2882.0
72	2046.0
73	1347.5
74	892.0
75	601.5
76	362.5
77	214.5
78	138.0
79	98.0
80	73.0
81	45.0
82	27.0
83	20.5
84	17.0
85	14.0
86	9.5
87	5.5
88	2.5
89	1.0
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007276134633336054
3	5.070477096401432E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.9016921510318834E-4
26-27	1.394749301420793E-4
28-29	0.0023845648638426147
30-31	0.010753183710462848
32-33	0.006054988967453925
34-35	4.84595166010189E-4
36-37	3.8399498656145546E-5
38-39	6.050609096794683E-4
40-41	0.0
42-43	0.0011997778845985816
44-45	6.17640550020737E-4
46-47	0.001458272268182103
48-49	0.0028754479011637675
50-51	0.00173962576199654
52-53	0.0
54-55	0.001492272697091232
56-57	0.04273297823737507
58-59	0.059785701320380094
60-61	0.0404416832611859
62-63	0.011365088683782613
64-65	0.0
66-67	0.0
68-69	1.5982383345310435E-4
70-71	0.0011441117263237812
72-73	0.0044417839803507315
74-75	0.0077685335548361364
76-77	0.015969801241572875
78-79	0.011726316305656029
80-81	0.005477168285534014
82-83	0.033352247445701186
84-85	0.02255137145922282
86-87	0.006354617445690998
88-89	0.0020011340827073517
90-91	0.003026104928812904
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	28.0
20-21	122.0
22-23	324.0
24-25	405.0
26-27	1014.0
28-29	2422.0
30-31	7313.0
32-33	9248.0
34-35	12509.0
36-37	20756.0
38-39	25265.0
40-41	24459.0
42-43	27720.0
44-45	32649.0
46-47	33571.0
48-49	31535.0
50-51	28680.0
52-53	27209.0
54-55	29025.0
56-57	30454.0
58-59	30275.0
60-61	29502.0
62-63	28920.0
64-65	30009.0
66-67	31667.0
68-69	32383.0
70-71	32366.0
72-73	30180.0
74-75	30832.0
76-77	31975.0
78-79	33475.0
80-81	33000.0
82-83	31425.0
84-85	30699.0
86-87	31671.0
88-89	32876.0
90-91	33946.0
92-93	33234.0
94-95	38402.0
96-97	120137.0
98-99	917922.0
100-101	1954798.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.55312143567811
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.01502167308374	81.48217388123136
2	5.8512096137035075	10.362857509357877
3	1.2269965872444881	3.2596313337427123
4	0.4191073379254907	1.4845305195959901
5	0.19801172880804202	0.8767278333413552
6	0.0932898061114814	0.49566621175805264
7	0.0521911597290397	0.32351830737481674
8	0.036965913444343135	0.2618757617774147
9	0.023539381900759347	0.18760371695808709
>10	0.08200035368894909	1.1122987301053502
>50	0.0011315494215502436	0.06591530532387281
>100	4.940315982475358E-4	0.0681671778664322
>500	4.0863340386012025E-5	0.019033711566652057
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.535238548200716E-5	0.0
5	0.0	0.0	0.0	2.535238548200716E-5	0.0
6	2.535238548200716E-5	0.0	0.0	2.535238548200716E-5	0.0
7	2.535238548200716E-5	0.0	0.0	2.535238548200716E-5	0.0
8	2.535238548200716E-5	0.0	0.0	2.535238548200716E-5	0.0
9	2.535238548200716E-5	7.605715644602148E-5	0.0	2.535238548200716E-5	0.0
10-11	2.535238548200716E-5	7.605715644602148E-5	0.0	2.535238548200716E-5	0.0
12-13	2.535238548200716E-5	7.605715644602148E-5	0.0	2.535238548200716E-5	0.0
14-15	2.535238548200716E-5	7.605715644602148E-5	0.0	3.802857822301074E-5	2.535238548200716E-5
16-17	7.605715644602148E-5	7.605715644602148E-5	0.0	5.070477096401432E-5	2.535238548200716E-5
18-19	7.605715644602148E-5	7.605715644602148E-5	0.0	5.070477096401432E-5	2.535238548200716E-5
20-21	7.605715644602148E-5	7.605715644602148E-5	0.0	5.070477096401432E-5	2.535238548200716E-5
22-23	7.605715644602148E-5	7.605715644602148E-5	0.0	7.605715644602148E-5	2.535238548200716E-5
24-25	7.605715644602148E-5	1.1408573466903221E-4	0.0	1.0140954192802863E-4	2.535238548200716E-5
26-27	7.605715644602148E-5	1.2676192741003579E-4	0.0	1.5211431289204296E-4	2.535238548200716E-5
28-29	7.605715644602148E-5	1.2676192741003579E-4	0.0	4.0563816771211454E-4	2.535238548200716E-5
30-31	7.605715644602148E-5	1.5211431289204296E-4	0.0	0.0013183240450643722	2.535238548200716E-5
32-33	7.605715644602148E-5	1.5211431289204296E-4	0.0	0.003574686352963009	2.535238548200716E-5
34-35	7.605715644602148E-5	1.9014289111505368E-4	0.0	0.006452182105170822	2.535238548200716E-5
36-37	1.0140954192802863E-4	2.0281908385605727E-4	0.0	0.010990259106450103	2.535238548200716E-5
38-39	1.0140954192802863E-4	2.2817146933806442E-4	0.0	0.018469212823642216	2.535238548200716E-5
40-41	1.0140954192802863E-4	2.5352385482007157E-4	0.0	0.02625239516661841	2.535238548200716E-5
42-43	1.5211431289204296E-4	2.6620004756107516E-4	0.0	0.0336426155346235	2.535238548200716E-5
44-45	1.5211431289204296E-4	3.2958101126609305E-4	0.0	0.042161017056577904	3.802857822301074E-5
46-47	1.5211431289204296E-4	3.8028578223010736E-4	0.0	0.050552656651122276	5.070477096401432E-5
48-49	1.5211431289204296E-4	3.8028578223010736E-4	0.0	0.05936261060611976	5.070477096401432E-5
50-51	2.0281908385605727E-4	4.81695324158136E-4	0.0	0.06856552653608836	5.070477096401432E-5
52-53	2.0281908385605727E-4	4.943715168991396E-4	0.0	0.07803464251361802	5.070477096401432E-5
54-55	2.1549527659706086E-4	5.197239023811467E-4	0.0	0.0864262821081624	5.070477096401432E-5
56-57	2.2817146933806442E-4	5.324000951221503E-4	0.0	0.09219394980531903	6.338096370501789E-5
58-59	2.2817146933806442E-4	5.450762878631539E-4	0.0	0.09816443658633171	1.0140954192802863E-4
60-61	2.2817146933806442E-4	5.577524806041575E-4	0.0	0.10433774245120046	1.0140954192802863E-4
62-63	2.5352385482007157E-4	5.577524806041575E-4	0.0	0.11020681969028512	1.0140954192802863E-4
64-65	2.9155243304308234E-4	6.591620225321861E-4	0.0	0.11602519215840576	1.0140954192802863E-4
66-67	3.0422862578408593E-4	7.098667934962005E-4	0.0	0.12161539315718833	1.0140954192802863E-4
68-69	3.0422862578408593E-4	7.352191789782076E-4	0.0	0.12666051786810778	1.0140954192802863E-4
70-71	3.0422862578408593E-4	7.478953717192111E-4	0.0	0.1320986045539983	1.0140954192802863E-4
72-73	3.2958101126609305E-4	7.732477572012183E-4	0.0	0.13626907196578847	1.2676192741003579E-4
74-75	3.2958101126609305E-4	8.112763354242291E-4	0.0	0.13962826304215442	1.2676192741003579E-4
76-77	3.2958101126609305E-4	8.619811063882434E-4	0.0	0.14218885397583714	1.2676192741003579E-4
78-79	3.2958101126609305E-4	8.873334918702505E-4	0.0	0.14406493050150568	1.2676192741003579E-4
80-81	3.2958101126609305E-4	8.873334918702505E-4	0.0	0.1448128258732249	1.2676192741003579E-4
82-83	3.2958101126609305E-4	9.000096846112541E-4	0.0	0.14538325454657003	1.2676192741003579E-4
84-85	3.2958101126609305E-4	9.126858773522577E-4	0.0	0.14573818794331816	1.2676192741003579E-4
86-87	3.4225720400709664E-4	9.126858773522577E-4	0.0	0.14586494987072818	1.2676192741003579E-4
88	3.8028578223010736E-4	9.380382628342649E-4	0.0	0.14587762606346918	1.2676192741003579E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5530	0.0	49.135036	1
GTATCAA	10120	0.0	37.218353	1
TCAACGC	12185	0.0	31.433643	4
ATCAACG	12255	0.0	31.428118	3
CAACGCA	12475	0.0	30.70292	5
TATCAAC	12715	0.0	30.59535	2
AACGCAG	12865	0.0	29.871632	6
ACGCAGA	14790	0.0	25.983673	7
CGCAGAG	15105	0.0	25.41357	8
GCAGAGT	17495	0.0	21.893051	9
TGGTATC	2705	0.0	21.13073	2
GAGTACT	10170	0.0	20.403553	12-13
GTGGTAT	3040	0.0	19.924696	1
CAGAGTA	17325	0.0	19.215141	10-11
GTACTTT	11075	0.0	18.582333	14-15
AGAGTAC	16145	0.0	17.911648	10-11
GTACATG	12545	0.0	17.341093	1
TACATGG	12845	0.0	16.902878	2
ACATGGG	12930	0.0	16.328661	3
AGTACTT	11065	0.0	16.305452	12-13
>>END_MODULE