FastQCFastQC Report
Fri 27 May 2016
SRR2139451_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139451_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4261914
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53200.050.0190161
GTATCAA97900.037.362831
TCAACGC115250.031.4363864
ATCAACG116850.031.1523633
CAACGCA117800.030.8285125
TATCAAC120450.030.4745222
AACGCAG121950.029.8144826
ACGCAGA143150.025.4289447
CGCAGAG148450.024.5498898
TGGTATC25650.022.1830182
GTGGTAT28250.021.201471
GCAGAGT171900.021.1262269
GAGTACT88500.019.72000512-13
CAGAGTA167700.018.67102410-11
GTACATG134800.018.4074861
TACATGG136300.018.1107462
ACATGGG135900.017.8150523
AGAGTAC152800.017.66428810-11
GTACTTT100650.017.29720314-15
CATGGGG102500.015.6911264
Produced by FastQC (version 0.11.5)