FastQCFastQC Report
Fri 27 May 2016
SRR2139450_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139450_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4908326
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68700.049.0370521
GTATCAA127750.038.0946161
TCAACGC152350.031.7142374
ATCAACG155200.031.3530433
TATCAAC157000.031.2433822
CAACGCA156600.030.880945
AACGCAG162750.029.6876436
ACGCAGA188850.025.5846677
CGCAGAG193200.024.9641938
GTGGTAT33050.023.2430461
TGGTATC32400.022.9146142
GCAGAGT219050.021.9790029
GAGTACT120050.020.1235512-13
CAGAGTA214900.019.20865610-11
GTACTTT131500.018.2736414-15
AGAGTAC200650.018.08143210-11
GTACATG162300.017.6103171
TACATGG164200.017.5894952
ACATGGG164200.017.0124243
AGTACTT130750.016.60608112-13
Produced by FastQC (version 0.11.5)