##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139450_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4908326
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.464161508424663	18.0	18.0	32.0	18.0	33.0
2	29.684983026799767	32.0	27.0	33.0	25.0	33.0
3	31.0144888501701	33.0	30.0	33.0	28.0	33.0
4	32.13656244511876	33.0	32.0	33.0	31.0	33.0
5	32.55355532619471	33.0	33.0	33.0	32.0	34.0
6	37.03018442540288	38.0	37.0	38.0	35.0	38.0
7	37.023422853331255	38.0	38.0	38.0	35.0	38.0
8	37.33018894832984	38.0	38.0	38.0	37.0	38.0
9	37.44833370888568	38.0	38.0	38.0	37.0	38.0
10-11	37.41788809463756	38.0	38.0	38.0	37.0	38.0
12-13	37.52248404038363	38.0	38.0	38.0	37.5	38.0
14-15	37.53239627930174	38.0	38.0	38.0	37.5	38.0
16-17	37.56336294695992	38.0	38.0	38.0	38.0	38.0
18-19	37.56458097934001	38.0	38.0	38.0	38.0	38.0
20-21	37.56750254445298	38.0	38.0	38.0	38.0	38.0
22-23	37.57356944336372	38.0	38.0	38.0	38.0	38.0
24-25	37.58138442515702	38.0	38.0	38.0	38.0	38.0
26-27	37.55253219297467	38.0	38.0	38.0	38.0	38.0
28-29	37.53008468354706	38.0	38.0	38.0	38.0	38.0
30-31	37.52630261949792	38.0	38.0	38.0	38.0	38.0
32-33	37.43886381872172	38.0	38.0	38.0	37.0	38.0
34-35	37.48431309719925	38.0	38.0	38.0	37.0	38.0
36-37	37.47167638220432	38.0	38.0	38.0	37.0	38.0
38-39	37.45750932426364	38.0	38.0	38.0	37.0	38.0
40-41	37.44808737690634	38.0	38.0	38.0	37.0	38.0
42-43	37.410698860746436	38.0	38.0	38.0	37.0	38.0
44-45	37.37162302023158	38.0	38.0	38.0	37.0	38.0
46-47	37.339485636698264	38.0	38.0	38.0	37.0	38.0
48-49	37.22332728138899	38.0	38.0	38.0	37.0	38.0
50-51	37.20700679627885	38.0	38.0	38.0	37.0	38.0
52-53	37.21299102921308	38.0	38.0	38.0	37.0	38.0
54-55	37.193298232148095	38.0	38.0	38.0	37.0	38.0
56-57	37.13903359222941	38.0	38.0	38.0	36.0	38.0
58-59	37.12067408092986	38.0	38.0	38.0	36.0	38.0
60-61	37.13364212689251	38.0	38.0	38.0	36.0	38.0
62-63	37.13578550924849	38.0	38.0	38.0	36.0	38.0
64-65	37.13891941438506	38.0	38.0	38.0	36.0	38.0
66-67	37.07318617688934	38.0	38.0	38.0	36.0	38.0
68-69	37.08865557267375	38.0	38.0	38.0	36.0	38.0
70-71	37.10080194382755	38.0	38.0	38.0	36.0	38.0
72-73	37.11202905797583	38.0	38.0	38.0	36.0	38.0
74-75	37.09772873216088	38.0	38.0	38.0	36.0	38.0
76-77	37.063648893497785	38.0	38.0	38.0	36.0	38.0
78-79	37.06142390808011	38.0	38.0	38.0	36.0	38.0
80-81	37.05837847652151	38.0	38.0	38.0	36.0	38.0
82-83	36.9987380870041	38.0	38.0	38.0	36.0	38.0
84-85	36.99213549520259	38.0	38.0	38.0	36.0	38.0
86-87	36.985603899653526	38.0	38.0	38.0	36.0	38.0
88-89	36.9609568113988	38.0	38.0	38.0	36.0	38.0
90-91	36.93412987550716	38.0	38.0	38.0	35.0	38.0
92-93	36.917455590850025	38.0	38.0	38.0	35.0	38.0
94-95	36.91023129276805	38.0	38.0	38.0	35.0	38.0
96-97	36.88278291609484	38.0	38.0	38.0	35.0	38.0
98-99	36.9032824831951	38.0	38.0	38.0	35.0	38.0
100	35.40228702579952	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	16.0
21	69.0
22	240.0
23	618.0
24	1526.0
25	3238.0
26	5905.0
27	10355.0
28	17247.0
29	25904.0
30	34474.0
31	44202.0
32	56342.0
33	75984.0
34	116930.0
35	226512.0
36	827071.0
37	3461689.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.8401055675601	23.275939699196833	14.541434289409464	23.3425204438336
2	14.433988502199007	20.566699165324444	41.68080205464869	23.318510277827865
3	18.365107926860574	25.70950898921913	30.61350473196146	25.311878351958832
4	13.066654496869198	17.981344352432988	38.63478098235529	30.31722016834253
5	13.775124146195669	37.09191280285784	35.13715266671366	13.995810384232833
6	30.101077230811484	37.86191055769319	18.531756040654187	13.505256170841138
7	26.8481759361542	32.725943631290995	22.712631557072616	17.71324887548219
8	24.256681402172553	37.329223853509326	20.702577620149924	17.7115171241682
9	25.49806186467647	17.70312322368156	21.517865765232383	35.28094914640959
10-11	24.522556162732467	27.167765547765164	28.14071436982792	20.168963919674447
12-13	25.269867975354533	24.501540851198556	28.74959813182743	21.478993041619486
14-15	22.449211808669595	25.844473248109434	26.475044648623587	25.231270294597387
16-17	21.829917165241266	29.053418619708633	27.17739204771647	21.93927216733363
18-19	21.82144177057514	28.02352573973285	29.128281210335256	21.026751279356752
20-21	22.708915938948703	27.07307427918536	29.062361330865116	21.155648451000822
22-23	22.436927397590175	26.949481609282987	28.995559154161082	21.618031838965752
24-25	22.12136845970244	27.14532436958078	29.048257394504954	21.685049776211823
26-27	22.043420559938255	27.27018001472315	29.022336905438138	21.664062519900455
28-29	22.00862151117489	27.22221514436368	29.087239929042486	21.68192341541894
30-31	22.236521308716327	27.202922196000994	28.936226516300806	21.624329978981873
32-33	22.036030153038986	27.37219627374005	28.88400747438918	21.707766098831783
34-35	22.18142414765555	27.31969759740183	28.934160401605723	21.56471785333689
36-37	22.151388829079785	27.24021125934748	28.897986039304502	21.710413872268237
38-39	22.01812129650162	27.35940474952969	28.983596678292738	21.638877275675952
40-41	22.248325942977274	27.25820978188193	28.84217085194783	21.651293423192968
42-43	22.15737439510204	27.347192342648093	28.632844325123695	21.86258893712617
44-45	22.176327226741734	27.462882985632657	28.561125708797675	21.79966407882793
46-47	22.314593507872086	27.454716056358073	28.253750452029635	21.97693998374021
48-49	22.217619019268632	27.591909032619235	28.182202887104147	22.00826906100799
50-51	22.204904745423917	27.703185642308053	28.107419581922148	21.984490030345874
52-53	22.417700698030956	27.641008427330256	27.972360078577395	21.96893079606139
54-55	22.329100099416387	27.59485145510186	28.022086837657888	22.053961607823865
56-57	22.30030843779227	27.732029740528613	28.004310579837888	21.96335124184123
58-59	22.262069364592822	27.77191019425714	27.99885603450146	21.967164406648575
60-61	22.342488818661092	27.760721814927926	28.055167104319235	21.84162226209175
62-63	22.297950488503638	27.865456661901238	28.026716543575432	21.8098763060197
64-65	22.268057721296174	27.779410150198004	27.998527676001256	21.95400445250457
66-67	22.367663489373236	27.903652337268493	27.827486860795048	21.901197312563227
68-69	22.252818099227955	27.644411728519923	28.103940716520455	21.99882945573167
70-71	22.379571731345465	27.429645545567492	28.094119710589784	22.09666301249726
72-73	22.365810295454853	27.66478492767785	27.982196353474254	21.987208423393042
74-75	22.220501548487327	27.776260781026107	27.927002909856352	22.07623476063021
76-77	22.336246437385476	27.528695177831374	28.006764989282885	22.128293395500265
78-79	22.269769800709867	27.557684368281045	27.896957835786797	22.275587995222292
80-81	22.285999015196886	27.634290193302185	27.90255908053709	22.177151710963834
82-83	22.401087108217233	27.526382560383123	27.959759788937045	22.112770542462602
84-85	22.411276923767755	27.518959521421767	27.952184585909084	22.117578968901398
86-87	22.28015836569064	27.5925875352524	27.969875834281094	22.15737826477587
88-89	22.35368525797708	27.59117378827754	27.895503572854285	22.15963738089109
90-91	22.39567021107272	27.622787741249798	27.91435407435551	22.06718797332197
92-93	22.455191015013195	27.5487906800839	27.874485557211642	22.121532747691266
94-95	22.2980842372479	27.633654368342548	27.90297719302592	22.16528420138363
96-97	22.48574951345157	27.74423826329156	27.844901161409	21.925111061847872
98-99	23.506636381821682	29.651839643497762	28.09623503965372	18.74528893502683
100	22.451056823431134	30.576588023758976	22.043464765883876	24.928890386926017
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1205.0
1	1123.5
2	1162.0
3	1364.5
4	1649.0
5	2189.0
6	2792.0
7	3745.0
8	4919.0
9	5723.5
10	5891.0
11	5940.5
12	6649.5
13	8301.5
14	11670.0
15	15709.0
16	18570.5
17	19756.0
18	19341.0
19	17833.5
20	15896.5
21	14468.5
22	14628.0
23	16905.0
24	21234.5
25	27824.5
26	36215.5
27	45658.5
28	55297.0
29	66423.5
30	77344.5
31	87624.0
32	100341.0
33	113135.0
34	125524.0
35	137716.5
36	150116.0
37	160998.5
38	168790.0
39	176247.0
40	184198.5
41	190208.0
42	193111.0
43	197675.0
44	202411.5
45	205576.0
46	206842.5
47	206168.5
48	205985.5
49	204247.5
50	200543.5
51	194684.5
52	187063.5
53	176960.5
54	165145.5
55	153705.0
56	140882.0
57	126422.5
58	112110.0
59	96934.5
60	80529.5
61	64950.0
62	51293.0
63	39964.5
64	30302.5
65	22237.5
66	16179.5
67	12012.5
68	9039.5
69	6591.0
70	4649.5
71	3167.5
72	2059.0
73	1320.5
74	853.0
75	543.0
76	355.0
77	214.5
78	137.5
79	90.0
80	54.5
81	36.5
82	28.5
83	18.0
84	10.5
85	8.5
86	7.5
87	6.0
88	4.5
89	2.5
90	1.0
91	0.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.008780997839181832
3	4.074708974098298E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.6488560303958265E-4
26-27	3.566148488309656E-4
28-29	0.0025683473408483312
30-31	0.01207710373915904
32-33	0.007255169947246739
34-35	5.53060014488124E-4
36-37	1.2332936247044335E-4
38-39	6.199798423887244E-4
40-41	1.039528594572933E-5
42-43	0.0016315114300971606
44-45	7.373105691152821E-4
46-47	0.0013912495289930028
48-49	0.0026554862131010553
50-51	0.0017914310242398961
52-53	0.0
54-55	0.0013793956604869378
56-57	0.046565150006304665
58-59	0.06536803214087646
60-61	0.04402626777320923
62-63	0.012045856519158788
64-65	0.0
66-67	0.0
68-69	2.6645334952133976E-4
70-71	0.0014850323935845665
72-73	0.005640768263196831
74-75	0.008462459233501151
76-77	0.017735614699354314
78-79	0.012301543680115264
80-81	0.005715594933461663
82-83	0.03599570572342842
84-85	0.024678706067632127
86-87	0.007386899893034669
88-89	0.002147147717537509
90-91	0.0032987046359122143
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	36.0
20-21	146.0
22-23	289.0
24-25	427.0
26-27	1060.0
28-29	2582.0
30-31	8350.0
32-33	10386.0
34-35	14466.0
36-37	24303.0
38-39	29482.0
40-41	28314.0
42-43	32154.0
44-45	38329.0
46-47	38993.0
48-49	37024.0
50-51	34331.0
52-53	32158.0
54-55	34236.0
56-57	36052.0
58-59	36268.0
60-61	35596.0
62-63	34310.0
64-65	35392.0
66-67	37804.0
68-69	39660.0
70-71	40031.0
72-73	36234.0
74-75	36675.0
76-77	37739.0
78-79	40015.0
80-81	39761.0
82-83	37166.0
84-85	36244.0
86-87	37068.0
88-89	39584.0
90-91	40514.0
92-93	40174.0
94-95	46056.0
96-97	149436.0
98-99	330349.0
100-101	3299132.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.23816739231899
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.69553606446182	80.91046060384403
2	6.102250952415193	10.769028820182996
3	1.298203890300304	3.436533965450338
4	0.41742763256662935	1.4733219726637463
5	0.19509062259974513	0.8607219506814021
6	0.09640571836148915	0.5103998348614703
7	0.057010074180713646	0.3521325128024434
8	0.036130296162511226	0.25504568965773805
9	0.023454266117938088	0.1862605313800755
>10	0.07644088953270542	1.060983014895155
>50	0.0015419268511159155	0.0937959935186058
>100	4.742569946455089E-4	0.07303757762512132
>500	3.3409455226046764E-5	0.018277532436877027
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.074708974098298E-5	0.0	2.037354487049149E-5	0.0
3	0.0	4.074708974098298E-5	0.0	2.037354487049149E-5	0.0
4	0.0	1.0186772435245744E-4	0.0	4.074708974098298E-5	0.0
5	0.0	1.2224126922294893E-4	0.0	4.074708974098298E-5	0.0
6	0.0	1.2224126922294893E-4	0.0	6.112063461147447E-5	0.0
7	0.0	1.2224126922294893E-4	0.0	6.112063461147447E-5	0.0
8	0.0	1.2224126922294893E-4	0.0	6.112063461147447E-5	0.0
9	0.0	1.629883589639319E-4	0.0	6.112063461147447E-5	0.0
10-11	0.0	1.629883589639319E-4	0.0	8.149417948196595E-5	0.0
12-13	0.0	1.629883589639319E-4	0.0	1.1205449678770319E-4	0.0
14-15	0.0	2.546693108811436E-4	0.0	1.2224126922294893E-4	0.0
16-17	0.0	2.648560833163893E-4	0.0	1.2224126922294893E-4	0.0
18-19	0.0	3.259767179278638E-4	0.0	1.2224126922294893E-4	0.0
20-21	0.0	3.8709735253933825E-4	0.0	1.2224126922294893E-4	0.0
22-23	0.0	4.176576698450755E-4	0.0	1.2224126922294893E-4	0.0
24-25	0.0	4.685915320213042E-4	0.0	1.629883589639319E-4	0.0
26-27	0.0	5.093386217622872E-4	0.0	2.342957660106521E-4	0.0
28-29	0.0	5.093386217622872E-4	0.0	4.7877830445654994E-4	0.0
30-31	0.0	5.704592563737617E-4	0.0	0.0014159613684991584	0.0
32-33	0.0	5.704592563737617E-4	0.0	0.0034023819933720785	2.037354487049149E-5
34-35	0.0	6.010195736794988E-4	0.0	0.005949075102183514	2.037354487049149E-5
36-37	0.0	6.315798909852361E-4	0.0	0.009809861855141652	2.037354487049149E-5
38-39	0.0	7.232608429024478E-4	0.0	0.015962672406030082	2.037354487049149E-5
40-41	0.0	7.94568249949168E-4	0.0	0.021616331107591466	2.037354487049149E-5
42-43	0.0	8.149417948196595E-4	0.0	0.027830262293091372	2.037354487049149E-5
44-45	0.0	8.862492018663797E-4	0.0	0.035103617811856835	2.037354487049149E-5
46-47	0.0	9.473698364778542E-4	0.0	0.04216305110948213	2.037354487049149E-5
48-49	0.0	9.575566089130999E-4	0.0	0.04880482673726236	2.037354487049149E-5
50-51	0.0	0.001151105285182769	0.0	0.055507722999654056	2.037354487049149E-5
52-53	0.0	0.0011612920576180147	0.0	0.06196613672359986	2.037354487049149E-5
54-55	0.0	0.0011816656024885063	0.0	0.0683430562680637	2.037354487049149E-5
56-57	0.0	0.0012020391473589978	0.0	0.07387447370040212	2.037354487049149E-5
58-59	0.0	0.0012020391473589978	0.0	0.07925308954621188	2.037354487049149E-5
60-61	0.0	0.0012224126922294894	0.0	0.08436684930870525	2.037354487049149E-5
62-63	0.0	0.0012427862370999808	0.0	0.08914444558083551	2.037354487049149E-5
64-65	0.0	0.001426148140934404	0.0	0.09408503021192968	2.037354487049149E-5
66-67	0.0	0.0014465216858048956	0.0	0.09972850214105583	2.037354487049149E-5
68-69	0.0	0.0014465216858048956	0.0	0.10450609841318609	2.037354487049149E-5
70-71	0.0	0.0014770820031106328	0.0	0.10899846505712946	2.037354487049149E-5
72-73	4.074708974098298E-5	0.00150764232041637	0.0	0.11253327509215973	2.037354487049149E-5
74-75	4.074708974098298E-5	0.001548389410157353	0.0	0.11547725232594575	2.037354487049149E-5
76-77	4.074708974098298E-5	0.0016095100447688275	0.0	0.11867589887061292	2.037354487049149E-5
78-79	4.074708974098298E-5	0.001629883589639319	0.0	0.12043821050191042	2.037354487049149E-5
80-81	4.074708974098298E-5	0.001629883589639319	0.0	0.12111053748263664	2.037354487049149E-5
82-83	4.074708974098298E-5	0.0016400703620745648	0.0	0.12144670097299975	2.037354487049149E-5
84-85	4.074708974098298E-5	0.0016502571345098104	0.0	0.12175230414605713	2.037354487049149E-5
86-87	4.074708974098298E-5	0.0016502571345098104	0.0	0.12179305123579812	2.037354487049149E-5
88	4.074708974098298E-5	0.001711377769121285	0.0	0.12179305123579812	2.037354487049149E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6870	0.0	49.037052	1
GTATCAA	12775	0.0	38.094616	1
TCAACGC	15235	0.0	31.714237	4
ATCAACG	15520	0.0	31.353043	3
TATCAAC	15700	0.0	31.243382	2
CAACGCA	15660	0.0	30.88094	5
AACGCAG	16275	0.0	29.687643	6
ACGCAGA	18885	0.0	25.584667	7
CGCAGAG	19320	0.0	24.964193	8
GTGGTAT	3305	0.0	23.243046	1
TGGTATC	3240	0.0	22.914614	2
GCAGAGT	21905	0.0	21.979002	9
GAGTACT	12005	0.0	20.12355	12-13
CAGAGTA	21490	0.0	19.208656	10-11
GTACTTT	13150	0.0	18.27364	14-15
AGAGTAC	20065	0.0	18.081432	10-11
GTACATG	16230	0.0	17.610317	1
TACATGG	16420	0.0	17.589495	2
ACATGGG	16420	0.0	17.012424	3
AGTACTT	13075	0.0	16.606081	12-13
>>END_MODULE